Beauveria bassiana no controle biol?gico de est?gios imaturos de Stomoxys calcitrans

Detalhes bibliográficos
Autor(a) principal: Badini, Ana Paula Rodrigues Moraes
Data de Publicação: 2011
Tipo de documento: Tese
Idioma: por
Título da fonte: Biblioteca Digital de Teses e Dissertações da UFRRJ
Texto Completo: https://tede.ufrrj.br/jspui/handle/jspui/2680
Resumo: Abstract: Stomoxys calcitrans (Linnaeus, 1758) population imbalance has caused significant economic losses in animal productivity. Currently concern about environmental safety has fostered the research and development for alternative control methods. The present study evaluated the entomopathogenic potential of Beauveria bassiana CG 138, CG 228 and ESALQ 986 isolates using three different in vitro methods to treat S. calcitrans eggs, larvae and pupae. S. calcitrans larvae exposed to CG 138 isolate and control group have been examined by scanning electron microscopy to verify the fungus adhesion and development on cuticle and natural openings. The in vitro antifungal activity was evaluated by diffusion test on media solidified using the larvae homogenate and surface mucus of control and treated groups. The bacterium present in the larvae homogenate and surface mucus was identified by PCR method. The antifungal activity of this bacterium was evaluated using the three B. bassiana isolates. The protein, enzymatic and chromatography profiles of these samples were evaluated by electrophoresis in a sodium dodecyl sulfate-polyacrylamide, zymography and high-performance liquid chromatography respectively. The results observed in the present study showed that the S. calcitrans immature stages are able to develop when are exposed to the three fungi isolates under experimental conditions. The three different fungi exposure methods used did not reduce the flies emergence of S. calcitrans adults. There was antifungal activity when the CG 138 isolate was exposed to the homogenate of larvae treated with this fungus. A reduced development of CG 138 on larvae cuticle was observed by scanning electron microscopy. There were differences between the chromatographic, protein and enzymatic profiles from control and treated groups, indicating antifungal reaction. The bacterium was identified as Stenotrophomonas maltophilia and presented antifungal activity against the three isolates. The results suggested the CG 138, 228, and ESALQ 986 isolates presented saprophytic effect since did not change significantly the development of S. calcitrans immature stages, and therefore, are not indicated to be used as stable fly biocontrol agent.
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spelling Bittencourt, Avelino Jos?647.137.097-00http://lattes.cnpq.br/7167155312867247Bittencourt, V?nia Rita Elias PinheiroSalles, Cristiane Martins CardosoMoraes, Aurea Maria Lage deCosta, Gisela Lara daMello, Rubens Pinto de077.184.757-27http://lattes.cnpq.br/9689712645766039Badini, Ana Paula Rodrigues Moraes2019-05-07T14:11:43Z2011-03-02BADINI, Ana Paula Rodrigues Moraes. Beauveria bassiana no controle biol?gico de est?gios imaturos de Stomoxys calcitrans. 2011. 97 f. Tese (Doutorado em Ci?ncias Veterin?rias) - Instituto de Veterin?ria, Universidade Federal Rural do Rio de Janeiro, Serop?dica, 2011.https://tede.ufrrj.br/jspui/handle/jspui/2680Abstract: Stomoxys calcitrans (Linnaeus, 1758) population imbalance has caused significant economic losses in animal productivity. Currently concern about environmental safety has fostered the research and development for alternative control methods. The present study evaluated the entomopathogenic potential of Beauveria bassiana CG 138, CG 228 and ESALQ 986 isolates using three different in vitro methods to treat S. calcitrans eggs, larvae and pupae. S. calcitrans larvae exposed to CG 138 isolate and control group have been examined by scanning electron microscopy to verify the fungus adhesion and development on cuticle and natural openings. The in vitro antifungal activity was evaluated by diffusion test on media solidified using the larvae homogenate and surface mucus of control and treated groups. The bacterium present in the larvae homogenate and surface mucus was identified by PCR method. The antifungal activity of this bacterium was evaluated using the three B. bassiana isolates. The protein, enzymatic and chromatography profiles of these samples were evaluated by electrophoresis in a sodium dodecyl sulfate-polyacrylamide, zymography and high-performance liquid chromatography respectively. The results observed in the present study showed that the S. calcitrans immature stages are able to develop when are exposed to the three fungi isolates under experimental conditions. The three different fungi exposure methods used did not reduce the flies emergence of S. calcitrans adults. There was antifungal activity when the CG 138 isolate was exposed to the homogenate of larvae treated with this fungus. A reduced development of CG 138 on larvae cuticle was observed by scanning electron microscopy. There were differences between the chromatographic, protein and enzymatic profiles from control and treated groups, indicating antifungal reaction. The bacterium was identified as Stenotrophomonas maltophilia and presented antifungal activity against the three isolates. The results suggested the CG 138, 228, and ESALQ 986 isolates presented saprophytic effect since did not change significantly the development of S. calcitrans immature stages, and therefore, are not indicated to be used as stable fly biocontrol agent.O desequil?brio populacional de Stomoxys calcitrans (Linnaeus, 1758) tem ocasionado perdas significativas na produ??o animal. Atualmente, pesquisas t?m sido direcionadas para m?todos de controle que preservem o meio ambiente. Com o presente estudo verificou-se o potencial entomopatog?nico dos isolados CG 138, CG 228 e ESALQ 986 de Beauveria bassiana sobre ovos, larvas e pupas de S. calcitrans onde se utilizou tr?s m?todos de exposi??o f?ngica. Larvas de S. calcitrans expostas ao isolado CG 138 e do grupo controle foram submetidas ? Microscopia Eletr?nica de Varredura para verificar a capacidade do fungo em aderir e se desenvolver sobre a cut?cula e aberturas naturais. Macerado e muco de larvas expostas aos tr?s isolados e as do grupo controle foram utilizados para verificar a capacidade de inibi??o f?ngica atrav?s do teste de difus?o em meio s?lido. Atrav?s do m?todo de PCR fez-se a identifica??o de uma bact?ria, presente no macerado e muco das larvas e foi avaliada a atividade antif?ngica para os tr?s isolados. Ainda foram avaliados os perfis cromatogr?ficos, prot?icos e proteol?ticos destas amostras em cromatografia l?quida de alta efici?ncia, eletroforese prot?ica e enzim?tica, respectivamente. Foi observado que os est?gios imaturos de S. calcitrans se desenvolveram em condi??es experimentais, onde foram expostos a diferentes concentra??es dos tr?s isolados f?ngicos. Os tr?s isolados n?o reduziram a emerg?ncia de moscas provenientes das larvas expostas nos tr?s m?todos utilizados. Inibi??o f?ngica foi observada quando o isolado CG 138 foi exposto ao macerado de larvas sensibilizadas ao mesmo. Atrav?s da microscopia eletr?nica de varredura, foi observado reduzido desenvolvimento do isolado CG 138 sobre a cut?cula das larvas. Verificaram-se diferen?as entre os perfis cromatogr?ficos, prot?icos e proteol?ticos de larvas expostas aos isolados f?ngicos e as do grupo controle, indicando rea??o antif?ngica. A bact?ria identificada como Stenotrophomonas maltophilia apresentou atividade antif?ngica frente aos tr?s isolados. Os resultados sugerem que os isolados CG 138, CG 228 e ESALQ 986 apresentaram efeito saprof?tico, pois n?o afetaram significativamente o desenvolvimento dos est?gios imaturos da mosca dos est?bulos, comprometendo desta forma a sua utiliza??o como agente entomopatog?nico no controle de S. calcitrans.Submitted by Sandra Pereira (srpereira@ufrrj.br) on 2019-05-07T14:11:43Z No. of bitstreams: 1 2011 - Ana Paula Rodrigues Moraes Badini.pdf: 2098313 bytes, checksum: 2a4352d355473a30e12b600f29da697c (MD5)Made available in DSpace on 2019-05-07T14:11:43Z (GMT). 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dc.title.por.fl_str_mv Beauveria bassiana no controle biol?gico de est?gios imaturos de Stomoxys calcitrans
title Beauveria bassiana no controle biol?gico de est?gios imaturos de Stomoxys calcitrans
spellingShingle Beauveria bassiana no controle biol?gico de est?gios imaturos de Stomoxys calcitrans
Badini, Ana Paula Rodrigues Moraes
Stomoxys calcitrans
Beauveria bassiana
controle biol?gico
Stomoxys calcitrans
Beauveria bassiana
biologic control
Medicina Veterin?ria
title_short Beauveria bassiana no controle biol?gico de est?gios imaturos de Stomoxys calcitrans
title_full Beauveria bassiana no controle biol?gico de est?gios imaturos de Stomoxys calcitrans
title_fullStr Beauveria bassiana no controle biol?gico de est?gios imaturos de Stomoxys calcitrans
title_full_unstemmed Beauveria bassiana no controle biol?gico de est?gios imaturos de Stomoxys calcitrans
title_sort Beauveria bassiana no controle biol?gico de est?gios imaturos de Stomoxys calcitrans
author Badini, Ana Paula Rodrigues Moraes
author_facet Badini, Ana Paula Rodrigues Moraes
author_role author
dc.contributor.advisor1.fl_str_mv Bittencourt, Avelino Jos?
dc.contributor.advisor1ID.fl_str_mv 647.137.097-00
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/7167155312867247
dc.contributor.advisor-co1.fl_str_mv Bittencourt, V?nia Rita Elias Pinheiro
dc.contributor.referee1.fl_str_mv Salles, Cristiane Martins Cardoso
dc.contributor.referee2.fl_str_mv Moraes, Aurea Maria Lage de
dc.contributor.referee3.fl_str_mv Costa, Gisela Lara da
dc.contributor.referee4.fl_str_mv Mello, Rubens Pinto de
dc.contributor.authorID.fl_str_mv 077.184.757-27
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/9689712645766039
dc.contributor.author.fl_str_mv Badini, Ana Paula Rodrigues Moraes
contributor_str_mv Bittencourt, Avelino Jos?
Bittencourt, V?nia Rita Elias Pinheiro
Salles, Cristiane Martins Cardoso
Moraes, Aurea Maria Lage de
Costa, Gisela Lara da
Mello, Rubens Pinto de
dc.subject.por.fl_str_mv Stomoxys calcitrans
Beauveria bassiana
controle biol?gico
topic Stomoxys calcitrans
Beauveria bassiana
controle biol?gico
Stomoxys calcitrans
Beauveria bassiana
biologic control
Medicina Veterin?ria
dc.subject.eng.fl_str_mv Stomoxys calcitrans
Beauveria bassiana
biologic control
dc.subject.cnpq.fl_str_mv Medicina Veterin?ria
description Abstract: Stomoxys calcitrans (Linnaeus, 1758) population imbalance has caused significant economic losses in animal productivity. Currently concern about environmental safety has fostered the research and development for alternative control methods. The present study evaluated the entomopathogenic potential of Beauveria bassiana CG 138, CG 228 and ESALQ 986 isolates using three different in vitro methods to treat S. calcitrans eggs, larvae and pupae. S. calcitrans larvae exposed to CG 138 isolate and control group have been examined by scanning electron microscopy to verify the fungus adhesion and development on cuticle and natural openings. The in vitro antifungal activity was evaluated by diffusion test on media solidified using the larvae homogenate and surface mucus of control and treated groups. The bacterium present in the larvae homogenate and surface mucus was identified by PCR method. The antifungal activity of this bacterium was evaluated using the three B. bassiana isolates. The protein, enzymatic and chromatography profiles of these samples were evaluated by electrophoresis in a sodium dodecyl sulfate-polyacrylamide, zymography and high-performance liquid chromatography respectively. The results observed in the present study showed that the S. calcitrans immature stages are able to develop when are exposed to the three fungi isolates under experimental conditions. The three different fungi exposure methods used did not reduce the flies emergence of S. calcitrans adults. There was antifungal activity when the CG 138 isolate was exposed to the homogenate of larvae treated with this fungus. A reduced development of CG 138 on larvae cuticle was observed by scanning electron microscopy. There were differences between the chromatographic, protein and enzymatic profiles from control and treated groups, indicating antifungal reaction. The bacterium was identified as Stenotrophomonas maltophilia and presented antifungal activity against the three isolates. The results suggested the CG 138, 228, and ESALQ 986 isolates presented saprophytic effect since did not change significantly the development of S. calcitrans immature stages, and therefore, are not indicated to be used as stable fly biocontrol agent.
publishDate 2011
dc.date.issued.fl_str_mv 2011-03-02
dc.date.accessioned.fl_str_mv 2019-05-07T14:11:43Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/doctoralThesis
format doctoralThesis
status_str publishedVersion
dc.identifier.citation.fl_str_mv BADINI, Ana Paula Rodrigues Moraes. Beauveria bassiana no controle biol?gico de est?gios imaturos de Stomoxys calcitrans. 2011. 97 f. Tese (Doutorado em Ci?ncias Veterin?rias) - Instituto de Veterin?ria, Universidade Federal Rural do Rio de Janeiro, Serop?dica, 2011.
dc.identifier.uri.fl_str_mv https://tede.ufrrj.br/jspui/handle/jspui/2680
identifier_str_mv BADINI, Ana Paula Rodrigues Moraes. Beauveria bassiana no controle biol?gico de est?gios imaturos de Stomoxys calcitrans. 2011. 97 f. Tese (Doutorado em Ci?ncias Veterin?rias) - Instituto de Veterin?ria, Universidade Federal Rural do Rio de Janeiro, Serop?dica, 2011.
url https://tede.ufrrj.br/jspui/handle/jspui/2680
dc.language.iso.fl_str_mv por
language por
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