Efeito do fluazuron sobre ovos, larvas e f?meas ingurgitadas de Rhipicephalus microplus

Detalhes bibliográficos
Autor(a) principal: Gaud?ncio, Fabr?cio Nascimento
Data de Publicação: 2016
Tipo de documento: Tese
Idioma: por
Título da fonte: Biblioteca Digital de Teses e Dissertações da UFRRJ
Texto Completo: https://tede.ufrrj.br/jspui/handle/jspui/1804
Resumo: The objective of this research was to evaluate the effect of the arthropod growth regulator fluazuron in the energetic metabolism of Rhipicephalus microplus. 6 stabled cattle divided into control and treated groups containing three animals each. A commercial formulation containing the active compound fluazuron was administered topically in the treated group. The engorged female ticks were collected from some days before (day 0) and after treatment of animals (4, 8, and 15 days). After collecting the engorged females, the following biological parameters were analyzed: weight of the engorged female, weight of the female after oviposition, laying period, laying weight, egg production rate, reproductive efficiency index, percentage of hatching and nutritional index. It was also evaluated the metabolic profile: in the hemolymph (concentration of glucose, total protein and organic acids - oxalic, pyruvic and lactic, enzymatic activity of LDH, ALT and AST); in the fat body (glycogen concentration and percentage of variation of neutral lipids); in eggs (concentration of glucose and total protein content, percentage change of neutral lipids and enzymatic activities of ALT and AST); and in the cuticle of engorged female ticks (percentage change of neutral lipids). Secondly, female immersion test was conducted to assess the activities of carboxylesterases enzymes, monooxygenases (MFO) and glutathione-S-transferase (GST) in sensitive strain of eggs and larvae (Porto Alegre, RS) compared with resistant strain (Jaguar, RS). It also assessed the detoxification enzymes in the fat body of engorged females of sensitive strain. The results showed that all the biological parameters showed lower results when compared to the control group after the exposure to fluazuron, except the weight of the female after oviposition. Although an increase was observed in the concentration of glucose in hemolymph on the 8th day and glycogen in the fat body in 15th day, there was no significant difference in the comparison of means between control and treated day-to-day. The concentration of lactic acid gradually increased in the treated group over time due to treatment, indicating increased anaerobic metabolism. There was no significant difference in the percentage change of neutral lipids in fat body, cuticle and eggs in any of the time intervals. No significant difference was observed in the activity of transaminases in the hemolymph, but it was observed in eggs after the treatment on the 8th day. The activity of MFO was higher in the comparison between the resistant and sensitive strains in eggs, but in larvae, the increase was observed in carboxylesterases in the resistant strain. In females, MFO showed high activity in the fat body as a function of fluazuron exposure, especially on day 4 in the treated group. Increases in transaminase activity and detoxification enzymes in females and eggs were associated with possible cell damage and trying to metabolism of active principle-tested. For the first time, a research about the metabolic effects of fluazuron has been conducted, focusing on different biochemical aspects, demonstrating that its effectiveness is related to a number of metabolic changes, leading to a parasite homeostasis breaking.
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spelling Silva, Jairo Pinheiro da477701710http://lattes.cnpq.br/8138213564144068Angelo, Isabele da Costa9034779718http://lattes.cnpq.br/5028095543336052Fonseca, Adivaldo Henrique daCid, Yara PelusoMonteiro, Caio M?rcio de oliveiraMelo, Raquel Moreira Pires dos Santos113.436.707-48http://lattes.cnpq.br/8138213564144068Gaud?ncio, Fabr?cio Nascimento2017-06-21T16:48:23Z2016-02-22GAUD?NCIO, Fabr?cio Nascimento. Efeito do fluazuron sobre ovos, larvas e f?meas ingurgitadas de Rhipicephalus microplus. 2016. 59 f. Tese (Doutorado em Ci?ncias Veterin?rias) - Instituto de Veterin?ria, Universidade Federal Rural do Rio de Janeiro, Serop?dica, 2016.https://tede.ufrrj.br/jspui/handle/jspui/1804The objective of this research was to evaluate the effect of the arthropod growth regulator fluazuron in the energetic metabolism of Rhipicephalus microplus. 6 stabled cattle divided into control and treated groups containing three animals each. A commercial formulation containing the active compound fluazuron was administered topically in the treated group. The engorged female ticks were collected from some days before (day 0) and after treatment of animals (4, 8, and 15 days). After collecting the engorged females, the following biological parameters were analyzed: weight of the engorged female, weight of the female after oviposition, laying period, laying weight, egg production rate, reproductive efficiency index, percentage of hatching and nutritional index. It was also evaluated the metabolic profile: in the hemolymph (concentration of glucose, total protein and organic acids - oxalic, pyruvic and lactic, enzymatic activity of LDH, ALT and AST); in the fat body (glycogen concentration and percentage of variation of neutral lipids); in eggs (concentration of glucose and total protein content, percentage change of neutral lipids and enzymatic activities of ALT and AST); and in the cuticle of engorged female ticks (percentage change of neutral lipids). Secondly, female immersion test was conducted to assess the activities of carboxylesterases enzymes, monooxygenases (MFO) and glutathione-S-transferase (GST) in sensitive strain of eggs and larvae (Porto Alegre, RS) compared with resistant strain (Jaguar, RS). It also assessed the detoxification enzymes in the fat body of engorged females of sensitive strain. The results showed that all the biological parameters showed lower results when compared to the control group after the exposure to fluazuron, except the weight of the female after oviposition. Although an increase was observed in the concentration of glucose in hemolymph on the 8th day and glycogen in the fat body in 15th day, there was no significant difference in the comparison of means between control and treated day-to-day. The concentration of lactic acid gradually increased in the treated group over time due to treatment, indicating increased anaerobic metabolism. There was no significant difference in the percentage change of neutral lipids in fat body, cuticle and eggs in any of the time intervals. No significant difference was observed in the activity of transaminases in the hemolymph, but it was observed in eggs after the treatment on the 8th day. The activity of MFO was higher in the comparison between the resistant and sensitive strains in eggs, but in larvae, the increase was observed in carboxylesterases in the resistant strain. In females, MFO showed high activity in the fat body as a function of fluazuron exposure, especially on day 4 in the treated group. Increases in transaminase activity and detoxification enzymes in females and eggs were associated with possible cell damage and trying to metabolism of active principle-tested. For the first time, a research about the metabolic effects of fluazuron has been conducted, focusing on different biochemical aspects, demonstrating that its effectiveness is related to a number of metabolic changes, leading to a parasite homeostasis breaking.O objetivo desta pesquisa foi avaliar os perfis metab?lico-energ?tico de carrapatos Rhipicephalus microplus expostos ao fluazuron. 6 bovinos foram estabulados e divididos em grupos controle e tratado, contendo 3 animais cada. Uma formula??o comercial contendo o princ?pio-ativo fluazuron foi administrada por via t?pica nos animais do grupo tratado. F?meas ingurgitadas foram coletadas em dias determinados: antes (dia 0) e ap?s o tratamento dos animais (4, 8 e 15 dias). Ap?s a coleta das f?meas ingurgitadas, foram analisados os seguintes par?metros biol?gicos: peso da f?mea ingurgitada, peso da quen?gina, per?odo de postura, peso da postura, ?ndice de produ??o de ovos, efici?ncia reprodutiva, percentual de eclos?o e ?ndice nutricional. Foi avaliado tamb?m o perfil metab?lico: na hemolinfa (concentra??o de glicose, prote?nas totais e ?cidos org?nicos - ox?lico, pir?vico e l?tico, atividade enzim?tica de LDH, ALT e AST), no corpo gorduroso (concentra??o de glicog?nio e percentual de varia??o de lip?dios neutros), nos ovos (concentra??o de glicose, prote?nas totais, percentual varia??o de lip?dios neutros e atividade enzim?tica de ALT e AST), e na cut?cula das f?meas ingurgitadas (percentual de varia??o de lip?dios neutros). Num segundo momento, realizou-se teste de imers?o de f?meas ingurgitadas para posterior avalia??o das atividades das enzimas carboxilesterases, monooxigenases (MFO) e glutationa-S-transferase (GST) em ovos e larvas de diferentes cepas: sens?vel (Porto Alegre, RS) e cepa resistente (Jaguar, RS) ao fluazuron. Avaliou-se tamb?m as enzimas de detoxifica??o no corpo gorduroso de f?meas ingurgitadas de cepa sens?vel. Na an?lise dos resultados, todos os par?metros apresentaram resultados inferiores, quando comparados ao grupo controle, em fun??o da exposi??o ao fluazuron, exceto o peso da quen?gina. Embora tenha sido observado um aumento na concentra??o de glicose hemolinf?tica no 8? dia e glicog?nio no corpo gorduroso no 15?dia, n?o houve diferen?a significativa na compara??o das m?dias entre controle e tratado dia-a-dia. A concentra??o do ?cido l?tico apresentou aumento progressivo significativo no grupo tratado ao longo do tempo em fun??o do tratamento, indicando aumento do metabolismo anaer?bio. N?o houve diferen?a significativa para as varia??es percentuais de lip?dios neutros no corpo gorduroso, cut?cula e ovos em nenhum dos dias de avalia??o. Embora n?o tenha sido observada diferen?a significativa nas atividades das transaminases na hemolinfa, observou-se aumento significativo nos ovos ap?s o tratamento no 8?dia. A atividade da MFO foi maior, na compara??o entre as cepas resistente e sens?vel, nos ovos, e com rela??o ?s larvas; o aumento observado foi nas carboxilesterases da cepa resistente. Nas f?meas, a MFO apresentou atividade elevada no corpo gorduroso em fun??o da exposi??o ao fluazuron, principalmente no 4?dia no grupo tratado. Os aumentos na atividade das transaminases e das enzimas de detoxifica??o nas f?meas ingurgitadas e nos ovos foram associados a poss?veis danos celulares e ? tentativa de metaboliza??o do princ?pio-ativo testado. Pela primeira vez, foi realizado um estudo acerca dos efeitos metab?licos do fluazuron, enfocando diferentes aspectos bioqu?micos, evidenciando que a sua efic?cia est? relacionada a diversas altera??es metab?licas, levando a uma quebra da homeostase do carrapatoSubmitted by Celso Magalhaes (celsomagalhaes@ufrrj.br) on 2017-06-21T16:48:23Z No. of bitstreams: 1 2016 - Fabr?cio Nascimento Gaud?ncio.pdf: 2705400 bytes, checksum: 84083c56a169de3a2c732060527db477 (MD5)Made available in DSpace on 2017-06-21T16:48:23Z (GMT). No. of bitstreams: 1 2016 - Fabr?cio Nascimento Gaud?ncio.pdf: 2705400 bytes, checksum: 84083c56a169de3a2c732060527db477 (MD5) Previous issue date: 2016-02-22CAPES - Coordena??o de Aperfei?oamento de Pessoal de N?vel Superiorapplication/pdfhttps://tede.ufrrj.br/retrieve/5591/2016%20-%20Fabr%c3%adcio%20Nascimento%20Gaud%c3%aancio.pdf.jpghttps://tede.ufrrj.br/retrieve/20334/2016%20-%20Fabr%c3%adcio%20Nascimento%20Gaud%c3%aancio.pdf.jpghttps://tede.ufrrj.br/retrieve/26655/2016%20-%20Fabr%c3%adcio%20Nascimento%20Gaud%c3%aancio.pdf.jpghttps://tede.ufrrj.br/retrieve/33036/2016%20-%20Fabr%c3%adcio%20Nascimento%20Gaud%c3%aancio.pdf.jpghttps://tede.ufrrj.br/retrieve/39462/2016%20-%20Fabr%c3%adcio%20Nascimento%20Gaud%c3%aancio.pdf.jpghttps://tede.ufrrj.br/retrieve/45824/2016%20-%20Fabr%c3%adcio%20Nascimento%20Gaud%c3%aancio.pdf.jpghttps://tede.ufrrj.br/retrieve/52242/2016%20-%20Fabr%c3%adcio%20Nascimento%20Gaud%c3%aancio.pdf.jpghttps://tede.ufrrj.br/retrieve/58720/2016%20-%20Fabr%c3%adcio%20Nascimento%20Gaud%c3%aancio.pdf.jpgporUniversidade Federal Rural do Rio de JaneiroPrograma de P?s-Gradua??o em Ci?ncias Veterin?riasUFRRJBrasilInstituto de Veterin?riaABBAS, R.Z.; ZAMAN, M. 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dc.title.por.fl_str_mv Efeito do fluazuron sobre ovos, larvas e f?meas ingurgitadas de Rhipicephalus microplus
dc.title.alternative.eng.fl_str_mv Efeito do fluazuron sobre ovos, larvas e f?meas ingurgitadas de Rhipicephalus microplus
title Efeito do fluazuron sobre ovos, larvas e f?meas ingurgitadas de Rhipicephalus microplus
spellingShingle Efeito do fluazuron sobre ovos, larvas e f?meas ingurgitadas de Rhipicephalus microplus
Gaud?ncio, Fabr?cio Nascimento
benzoylphenylureas
growth regulator
energetic metabolism
benzoilfenilur?ias, ,
regulador de crescimento
metabolismo energ?tico
Parasitologia
title_short Efeito do fluazuron sobre ovos, larvas e f?meas ingurgitadas de Rhipicephalus microplus
title_full Efeito do fluazuron sobre ovos, larvas e f?meas ingurgitadas de Rhipicephalus microplus
title_fullStr Efeito do fluazuron sobre ovos, larvas e f?meas ingurgitadas de Rhipicephalus microplus
title_full_unstemmed Efeito do fluazuron sobre ovos, larvas e f?meas ingurgitadas de Rhipicephalus microplus
title_sort Efeito do fluazuron sobre ovos, larvas e f?meas ingurgitadas de Rhipicephalus microplus
author Gaud?ncio, Fabr?cio Nascimento
author_facet Gaud?ncio, Fabr?cio Nascimento
author_role author
dc.contributor.advisor1.fl_str_mv Silva, Jairo Pinheiro da
dc.contributor.advisor1ID.fl_str_mv 477701710
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/8138213564144068
dc.contributor.advisor-co1.fl_str_mv Angelo, Isabele da Costa
dc.contributor.advisor-co1ID.fl_str_mv 9034779718
dc.contributor.advisor-co1Lattes.fl_str_mv http://lattes.cnpq.br/5028095543336052
dc.contributor.referee1.fl_str_mv Fonseca, Adivaldo Henrique da
dc.contributor.referee2.fl_str_mv Cid, Yara Peluso
dc.contributor.referee3.fl_str_mv Monteiro, Caio M?rcio de oliveira
dc.contributor.referee4.fl_str_mv Melo, Raquel Moreira Pires dos Santos
dc.contributor.authorID.fl_str_mv 113.436.707-48
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/8138213564144068
dc.contributor.author.fl_str_mv Gaud?ncio, Fabr?cio Nascimento
contributor_str_mv Silva, Jairo Pinheiro da
Angelo, Isabele da Costa
Fonseca, Adivaldo Henrique da
Cid, Yara Peluso
Monteiro, Caio M?rcio de oliveira
Melo, Raquel Moreira Pires dos Santos
dc.subject.eng.fl_str_mv benzoylphenylureas
growth regulator
energetic metabolism
topic benzoylphenylureas
growth regulator
energetic metabolism
benzoilfenilur?ias, ,
regulador de crescimento
metabolismo energ?tico
Parasitologia
dc.subject.por.fl_str_mv benzoilfenilur?ias, ,
regulador de crescimento
metabolismo energ?tico
dc.subject.cnpq.fl_str_mv Parasitologia
description The objective of this research was to evaluate the effect of the arthropod growth regulator fluazuron in the energetic metabolism of Rhipicephalus microplus. 6 stabled cattle divided into control and treated groups containing three animals each. A commercial formulation containing the active compound fluazuron was administered topically in the treated group. The engorged female ticks were collected from some days before (day 0) and after treatment of animals (4, 8, and 15 days). After collecting the engorged females, the following biological parameters were analyzed: weight of the engorged female, weight of the female after oviposition, laying period, laying weight, egg production rate, reproductive efficiency index, percentage of hatching and nutritional index. It was also evaluated the metabolic profile: in the hemolymph (concentration of glucose, total protein and organic acids - oxalic, pyruvic and lactic, enzymatic activity of LDH, ALT and AST); in the fat body (glycogen concentration and percentage of variation of neutral lipids); in eggs (concentration of glucose and total protein content, percentage change of neutral lipids and enzymatic activities of ALT and AST); and in the cuticle of engorged female ticks (percentage change of neutral lipids). Secondly, female immersion test was conducted to assess the activities of carboxylesterases enzymes, monooxygenases (MFO) and glutathione-S-transferase (GST) in sensitive strain of eggs and larvae (Porto Alegre, RS) compared with resistant strain (Jaguar, RS). It also assessed the detoxification enzymes in the fat body of engorged females of sensitive strain. The results showed that all the biological parameters showed lower results when compared to the control group after the exposure to fluazuron, except the weight of the female after oviposition. Although an increase was observed in the concentration of glucose in hemolymph on the 8th day and glycogen in the fat body in 15th day, there was no significant difference in the comparison of means between control and treated day-to-day. The concentration of lactic acid gradually increased in the treated group over time due to treatment, indicating increased anaerobic metabolism. There was no significant difference in the percentage change of neutral lipids in fat body, cuticle and eggs in any of the time intervals. No significant difference was observed in the activity of transaminases in the hemolymph, but it was observed in eggs after the treatment on the 8th day. The activity of MFO was higher in the comparison between the resistant and sensitive strains in eggs, but in larvae, the increase was observed in carboxylesterases in the resistant strain. In females, MFO showed high activity in the fat body as a function of fluazuron exposure, especially on day 4 in the treated group. Increases in transaminase activity and detoxification enzymes in females and eggs were associated with possible cell damage and trying to metabolism of active principle-tested. For the first time, a research about the metabolic effects of fluazuron has been conducted, focusing on different biochemical aspects, demonstrating that its effectiveness is related to a number of metabolic changes, leading to a parasite homeostasis breaking.
publishDate 2016
dc.date.issued.fl_str_mv 2016-02-22
dc.date.accessioned.fl_str_mv 2017-06-21T16:48:23Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/doctoralThesis
format doctoralThesis
status_str publishedVersion
dc.identifier.citation.fl_str_mv GAUD?NCIO, Fabr?cio Nascimento. Efeito do fluazuron sobre ovos, larvas e f?meas ingurgitadas de Rhipicephalus microplus. 2016. 59 f. Tese (Doutorado em Ci?ncias Veterin?rias) - Instituto de Veterin?ria, Universidade Federal Rural do Rio de Janeiro, Serop?dica, 2016.
dc.identifier.uri.fl_str_mv https://tede.ufrrj.br/jspui/handle/jspui/1804
identifier_str_mv GAUD?NCIO, Fabr?cio Nascimento. Efeito do fluazuron sobre ovos, larvas e f?meas ingurgitadas de Rhipicephalus microplus. 2016. 59 f. Tese (Doutorado em Ci?ncias Veterin?rias) - Instituto de Veterin?ria, Universidade Federal Rural do Rio de Janeiro, Serop?dica, 2016.
url https://tede.ufrrj.br/jspui/handle/jspui/1804
dc.language.iso.fl_str_mv por
language por
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