Efic?cia e seguran?a cl?nica comparativa de duas formula??es de aplica??o t?pica contendo 10% de fipronil no controle de ectoparasitos em c?es e gatos.

The objectives of the present study were to evaluate the efficacy and clinical safety of a novel topical 10% fipronil on the control of Ctenocephalides felis felis on dogs and cats and Rhipicephalus sanguineus on dogs; residual efficacy of the fipronil formulation on dog s coat on the control of egg...

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Access type:openAccess
Publication Date:2009
Main Author: Tancredi, Michelle Goldan de Freitas lattes
Advisor: Scott, Fabio Barbour lattes
Document type: Doctoral thesis
Language:por
Published: Universidade Federal Rural do Rio de Janeiro
Program: Curso de P?s-Gradua??o em Ci?ncias Veterin?rias
Department: Parasitologia Veterin?ria
Portuguese subjects:
English subjects:
Knowledgement areas:
Online Access:https://tede.ufrrj.br/jspui/handle/tede/836
Citation:TANCREDI, Michelle Goldan de Freitas. Comparative efficacy and clinical safety of two topical formulations containing 10% fipronil on the control of ectoparasites on dogs and cats.. 2009. 76 f. Tese (Doutorado em Parasitologia Veterin?ria) - Universidade Federal Rural do Rio de Janeiro, Serop?dica, 2009.
Portuguese abstract:Os objetivos do presente estudo foram avaliar: a efic?cia e seguran?a cl?nica de uma formula??o t?pica de fipronil a 10% no controle de Ctenocephalides felis felis em c?es e gatos e Rhipicephalus sanguineus em c?es; a efic?cia residual de uma nova formula??o em teste de fipronil em pelo de c?es no controle de ovos, larvas e adultos de C. felis felis; o efeito da dispers?o da formula??o teste em pelo de c?es tratados sobre adultos de C. felis felis; e influ?ncia do banho sobre a efic?cia pulicida e carrapaticida em c?es da nova formula??o. Os animais utilizados pertenciam ao Laborat?rio de Quimioterapia Experimental em Parasitologia Veterin?ria (LQEPV), do Departamento de Parasitologia Animal, Instituto de Veterin?ria, da Universidade Federal Rural do Rio de Janeiro ou domiciliados. Pulgas e carrapatos eram provenientes de col?nias mantidas no LQEPV. As metodologias foram baseadas em normas ou literatura. A efic?cia pulicida em gatos foi: 99,4; 100; 100; 95,1; 86,8 e 70% para os dias +2 +7, +14, +21; +28, +35 e +42, respectivamente. A efic?cia carrapaticida em c?es foi de 100; 100; 95,4; 90,2; 83,4; 62,6% para os dias +2 +7, +14, +21; +28 e +35, respectivamente. A efic?cia pulicida em c?es foi: 100; 100; 100; 99,5; 99,8; 91,6% para os dias +2 +7, +14, +21; +28 e +35, respectivamente. Foi constatado que a formula??o em teste obteve satisfat?rios n?veis de efic?cia e esperado per?odo residual contra reinfesta??es. Em rela??o ? influ?ncia do banho na atividade pulicida, a efic?cia foi de 100% para os dias +3, +7, +14, +21 e +28, e 98,4; 95 e 70,6% para os dias 35, +42 e +49, respectivamente no grupo com banho ?nico. Para o grupo com banho semanal, a efic?cia foi de: 98,8; 100; 100; 100; 86,9 e 44,2%, para os dias 3, +7, +14, +21,+28 e +35, respectivamente. A efic?cia carrapaticida no grupo com banho ?nico foi de: 99,5; 100; 100; 91,8; 91,6; 70,9 e 63,2% para os dias +3, + 7, +14, +21, +28, +35 e +42, respectivamente. No grupo banho semanal as efic?cias foram de 100; 100; 99,1; 81,1; 58,9 e 41,3 para os dias +3, +7, +14, +21, +28 e +35, respectivamente. O banho ?nico demonstrou n?o provocar queda da efic?cia do produto. Banhos semanais, apesar de apresentarem efic?cia satisfat?ria, demonstraram menor per?odo residual. Para os experimentos in vitro avaliando atividade residual sobre pulgas pode concluir-se que a efic?cia: adulticida variou de mediana ? alta at? dia +14; a larvicida foi irregular, apresentando curta atividade residual; e a ovicida foi elevada, apresentando longo per?odo residual. Nos ensaios in vitro avaliando a velocidade de morte a formula??o teste foi 100% eficaz em at? 16h at? o dia +21 e 24h nos dias +28 e +35, demonstrando prolongada atividade residual. Portanto, a formula??o teste de aplica??o t?pica de fipronil 10% alcan?ou, em geral, satisfat?rios n?veis de efic?cia e atividade residual sobre C. felis felis em c?es e gatos e R. sanguineus em c?es, compar?veis ? formula??o refer?ncia existente no mercado, al?m de apresentar seguran?a cl?nica desejada.
English abstract:The objectives of the present study were to evaluate the efficacy and clinical safety of a novel topical 10% fipronil on the control of Ctenocephalides felis felis on dogs and cats and Rhipicephalus sanguineus on dogs; residual efficacy of the fipronil formulation on dog s coat on the control of eggs, larvae and adults of C. felis felis; effect of dispersion of the tested formulation on adults of C. felis felis; and the influence of bathe on the efficacy of the novel formulation against fleas and ticks on dogs. Utilized animals were belong to the the Laboratory of Experimental Chemotherapy in Veterinary Parasitology (LECVP) from the Department of Animal Parasitology of the Institute of Veterinary of the Universidade Federal Rural do Rio de Janeiro or domiciliated ones. Fleas and ticks were gathered from colonies maintained in the LECVP. Methodologies were based on guidelines and the literature. Pulicide efficacy on cats was: 99.4; 100; 100; 95.1; 86.8 and 70% on days +2 +7, +14, +21; +28, +35 e +42, respectively. The efficacy against ticks on dogs was: 100; 100; 95.4; 90.2; 83.4 and 62.6% on days +2 +7, +14, +21; +28 e +35, respectively. Pulicide efficacy on dogs was: 100; 100; 100; 99.5; 99.8 and 91.6% on days +2 +7, +14, +21; +28 e +35, respectively. Those essays demonstrated satisfactory efficacy levels obtained by the formulation and expected residual period against reinfestations. Regarding the influence of shampooing on pulicide activity, the efficacy was 100% on days +3, +7, +14, +21 and +28; 98.4; 95 and 70.6% on days +35, +42 e +49, respectively for the group with only one bathe. The weekly shampooing group presented efficacy of 98.8; 100; 100; 100; 86.9 and 44.2% on days +3, + 7, +14, +21, +28, +35 and +42, respectively. Efficacy against ticks for the group shampooed once was 99.5; 100; 100; 91.8; 91.6; 70.9 and 63.2% on days +3, + 7, +14, +21, +28, +35 and +42, respectively. The weekly shampooing group presented efficacy of 100; 100; 99.1; 81.1; 58.9 e 41.% on days +2 +7, +14, +21; +28 and +35, respectively. Shampooing once did not affect the product s efficacy. Weekly bathes, despite showed satisfactory efficacy, presented shorter residual period. Regarding the in vitro trials to evaluate the residual activity against fleas, the following can be concluded: the adulticide efficacy varied from average to high until day +14; larvicide efficacy was irregular and presented short residual activity; efficacy against eggs was high and presented long residual activity. Regarding the in vitro essays to evaluate speed of kill the formulation was 100% effective within 16h until day +21 and, 24h on days +28 and +35, demonstrating long residual activity. Therefore, the topical 10% fipronil formulation presented, in general, satisfactory efficacy levels and residual activity on C. felis felis on cats and dogs and, R. sanguineus on dogs, comparable to the reference formulation found commercially, besides have presented the desirable clinical safety.