Clonagem e express?o da prote?na recombinante p27 (r-p27) do v?rus da leucemia felina e produ??o de IgY avi?ria contra a r-p27 para utiliza??o em imunodiagn?stico
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2013 |
| Tipo de documento: | Tese |
| Idioma: | por |
| Título da fonte: | Biblioteca Digital de Teses e Dissertações da UFRRJ |
| Texto Completo: | https://tede.ufrrj.br/jspui/handle/jspui/2578 |
Resumo: | Feline Leukemia Virus (FeLV) is a gammaretrovirus, which represents an important cause of morbidity and mortality among domestic cats. In Brazil, the access to feline leukemia diagnosis is still limited due to the high cost of available commercial test to detect the viral antigen p27. The production of avian IgY instead of mammal IgG has several advantages, like lower costs of the technique, easier animal maintenance, high yields of purified antibodies and better acceptance due to bioethical characteristics. The aim of this study was the development of a recombinant viral p27 protein, the r-p27, to elicit avian antibodies, aiming to enable immunodiagnosis of FeLV infections. This technology can provide access to diagnosis and better understanding of prevalence studies. For this purpose, the coding region of the FeLV p27 protein was cloned and expressed in Escherichia coli and the reactivity against anti-p27 antibody was proven before commercial immunochromatography kit. To obtain IgG and IgY anti-p27, r-p27 was inoculated into rabbits and chickens, respectively, and the antibodies produced were tested by indirect immunofluorescence (IFA). Both immunoglobulins showed reactivity against recombinant and native p27. The production of IgY against r-p27 represents a method of high yields of purified antibodies and low cost, promising for the development of immunodiagnosis |
| id |
UFRRJ-1_f0b64c2f5292ed494d8321e47bbe094e |
|---|---|
| oai_identifier_str |
oai:localhost:jspui/2578 |
| network_acronym_str |
UFRRJ-1 |
| network_name_str |
Biblioteca Digital de Teses e Dissertações da UFRRJ |
| repository_id_str |
|
| spelling |
Mazur, Carlos610.764.147-53http://lattes.cnpq.br/7059968849728033Danelli, Maria das Gra?as Miranda758.643.637-15http://lattes.cnpq.br/7414712085904286Hagiwara, Mitika KuribayashiReis, Jenner Karlisson Pimenta dosMcIntosh, DouglasSantos, D?bora Regina Lopes dos086.087.827-90http://lattes.cnpq.br/4292226842530490Almeida, Nadia Rossi de2019-01-14T11:40:16Z2013-02-20ALMEIDA, Nadia Rossi de. Clonagem e express?o da prote?na recombinante p27 (r-p27) do v?rus da leucemia felina e produ??o de IgY avi?ria contra a r-p27 para utiliza??o em imunodiagn?stico. 2013. 87 f. Tese (Doutorado em Ci?ncias Veterin?rias). Instituto de Veterin?ria, Universidade Federal Rural do Rio de Janeiro, Serop?dica, 2013https://tede.ufrrj.br/jspui/handle/jspui/2578Feline Leukemia Virus (FeLV) is a gammaretrovirus, which represents an important cause of morbidity and mortality among domestic cats. In Brazil, the access to feline leukemia diagnosis is still limited due to the high cost of available commercial test to detect the viral antigen p27. The production of avian IgY instead of mammal IgG has several advantages, like lower costs of the technique, easier animal maintenance, high yields of purified antibodies and better acceptance due to bioethical characteristics. The aim of this study was the development of a recombinant viral p27 protein, the r-p27, to elicit avian antibodies, aiming to enable immunodiagnosis of FeLV infections. This technology can provide access to diagnosis and better understanding of prevalence studies. For this purpose, the coding region of the FeLV p27 protein was cloned and expressed in Escherichia coli and the reactivity against anti-p27 antibody was proven before commercial immunochromatography kit. To obtain IgG and IgY anti-p27, r-p27 was inoculated into rabbits and chickens, respectively, and the antibodies produced were tested by indirect immunofluorescence (IFA). Both immunoglobulins showed reactivity against recombinant and native p27. The production of IgY against r-p27 represents a method of high yields of purified antibodies and low cost, promising for the development of immunodiagnosisO V?rus da Leucemia Felina (FeLV) ? um membro da fam?lia Retroviridae, g?nero Gammaretrovirus, que representa uma importante causa de morbidade e mortalidade entre os gatos dom?sticos. No Brasil, o acesso ao diagn?stico da Leucemia Viral Felina ainda ? restrito devido ao alto custo do teste comercial dispon?vel, que detecta o ant?geno viral p27. Para utiliza??o em imunodiagn?stico, a IgY avi?ria apresenta uma s?rie de vantagens sobre os anticorpos IgG de mam?feros, como o baixo custo da t?cnica e manuten??o dos animais, alto rendimento dos anticorpos purificados e car?ter bio?tico. O objetivo do presente estudo foi o desenvolvimento de uma prote?na de nucleocaps?deo viral p27 recombinante, a r-p27, para elicitar anticorpos avi?rios, visando contribuir com o imunodiagn?stico das infec??es pelo FeLV no pa?s. Esta tecnologia poder? possibilitar o acesso ao diagn?stico e a melhor compreens?o dos estudos de preval?ncia da infec??o. Para tanto, a regi?o codificante da p27 nativa do FeLV foi amplificada, clonada e expressa em Escherichia coli, e sua reatividade contra os anticorpos para p27 nativa foi verificada com o kit comercial de imunocromatografia. A r-p27 foi inoculada em coelhos e galinhas, para produ??o de IgG e IgY espec?ficas, respectivamente. Estes anticorpos foram avaliados pela t?cnica de imunofluoresc?ncia indireta (IFI) contra a p27 nativa, em esfrega?os de sangue de animais positivos e negativos para o FeLV. Ambos os tipos de imunoglobulinas apresentaram reatividade para a p27 recombinante e nativa do FeLV. A produ??o de IgY para a r-p27 representou um m?todo de alto rendimento e baixo custo, promissor para o desenvolvimento de imunodiagn?stico da Leucemia Viral FelinaSubmitted by Celso Magalhaes (celsomagalhaes@ufrrj.br) on 2019-01-14T11:40:15Z No. of bitstreams: 1 2013 - Nadia Rossi de Almeida.pdf: 2617695 bytes, checksum: eca189b4390620fb87d9fbcf17c54ce6 (MD5)Made available in DSpace on 2019-01-14T11:40:16Z (GMT). No. of bitstreams: 1 2013 - Nadia Rossi de Almeida.pdf: 2617695 bytes, checksum: eca189b4390620fb87d9fbcf17c54ce6 (MD5) Previous issue date: 2013-02-20Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPESapplication/pdfhttps://tede.ufrrj.br/retrieve/9223/2013%20-%20Nadia%20Rossi%20de%20Almeida.pdf.jpghttps://tede.ufrrj.br/retrieve/15644/2013%20-%20Nadia%20Rossi%20de%20Almeida.pdf.jpghttps://tede.ufrrj.br/retrieve/21946/2013%20-%20Nadia%20Rossi%20de%20Almeida.pdf.jpghttps://tede.ufrrj.br/retrieve/28330/2013%20-%20Nadia%20Rossi%20de%20Almeida.pdf.jpghttps://tede.ufrrj.br/retrieve/34744/2013%20-%20Nadia%20Rossi%20de%20Almeida.pdf.jpghttps://tede.ufrrj.br/retrieve/41140/2013%20-%20Nadia%20Rossi%20de%20Almeida.pdf.jpghttps://tede.ufrrj.br/retrieve/47542/2013%20-%20Nadia%20Rossi%20de%20Almeida.pdf.jpghttps://tede.ufrrj.br/retrieve/53980/2013%20-%20Nadia%20Rossi%20de%20Almeida.pdf.jpgporUniversidade Federal Rural do Rio de JaneiroPrograma de P?s-Gradua??o em Ci?ncias Veterin?riasUFRRJBrasilInstituto de Veterin?riaAKITA, E.M.; NAKAI, S. Immunoglobulins from egg yolk: isolation and purification. Journal of Food Science, v. 57, p. 629-634, 1992. AKITA, E.M.; NAKAI, S. Comparison of four purification methods for the production of immunoglobulins from eggs laid by hens immunized with an enterotoxigenic E. coli strain. Journal of Immunology Methods, v. 160, n. 2, p. 207-214, 1993. ALMEIDA, M.O. Clonagem e express?o do gene da toxina beta de Clostridium perfringens tipo B e sua aplica??o na imuniza??o de animais. 2010, 97p. Disserta??o (Mestrado em Gen?tica). Universidade Federal de Minas Gerais, Belo Horizonte, 2010. ALMEIDA, N.R.; DANELLI, M.G.M.; SILVA, L.H.P.; HAGIWARA, M.K., MAZUR, C. Prevalence of feline leukemia virus infection in domestic cats in Rio de Janeiro. Journal of Feline Medicine and Surgery, v. 14, n. 8, p. 583-586, 2012. ALMEIDA, N.R; ALVES, R.S.; CASTRO, A.M.T.; VALENTIM, L.F.; PISTELLI, A.L. Ocorr?ncia de linfomas em gatos com Leucemia Viral Felina atendidos no Hospital Veterin?rio de Pequenos Animais (HVPA) da UFRRJ. In: Anais da 9a Confer?ncia Sul-americana de Medicina Veterin?ria, Rio de janeiro, 2009. ALVES, FABIANA. Padroniza??o de um ELISA indireto para o diagn?stico da imunodefici?ncia felina a v?rus. 2010, 32p. Disserta??o (Mestrado em Ci?ncia Animal). Universidade Federal de Minas Gerais, Belo horizonte, 2010. ANAI, Y.; OCHI, H.; WATANABE, S., NAKAGAWA, S.; KAWAMURA, M.; GOJOBORI, T.; NISHIGAKI, K. Infectious Endogenous Retroviruses in Cats and Emergence of Recombinant Viruses. Journal of Virology, v. 86, n.16, p. 8634-8644, 2012. ARA?JO, A.S. Produ??o de antiveneno botr?pico em ovos de galinha. 2007, 57p. Disserta??o (Mestrado em Medicina Veterin?ria Preventiva). Universidade Federal de Minas Gerais, Belo Horizonte, 2007. ARA?JO, M.R.T. Express?o de prote?nas recombinantes de v?rus do g?nero Flavivirus: Aplica??o no desenvolvimento de kits de diagn?stico e em estrat?gias antivirais. 2009, 172p. Disserta??o (Mestrado em Biologia Celular e Molecular). Universidade Federal do Paran?, Curitiba, 2009. ARJONA, A.; ESCOLAR, E.; SOTO, I; BARQUERO, N.; MARTIN, D. Seroepidemiological Survey of Infection by Feline Leukemia Virus and immunodeficiency Virus in Madrid and Correlation with Some Clinical Aspects. Journal of Clinical Microbiology, v. 38, n. 9, p. 3448-3449, 2000. AUDIBERT, F. M.; LISE, L. D. Adjuvants: Current Status, Clinical Perspectives and Future Prospects. Immunology Today, v. 14, p. 281-284, 1993. AUDIBERT, F. Adjuvants for vaccines, a quest. International Immunopharmacology, v. 3, p. 1187-1193, 2003. 55 AUGUST, J.R. Mol?stias virais felinas. In: ETTINGER, S. J. Tratado de Medicina Interna Veterin?ria. 3.ed.S?o Paulo: Manole, 1992. cap.48. p. 340-346. BALAMURUGAN, V.; VENKATESAN, G.; SEN, A.; ANNAMALAI, L.; BHANUPRAKASH, V.; SINGH, R.K. Recombinant protein-based viral disease diagnostics in veterinary medicine. Expert Review of Molecular Diagnostics, v. 10, n. 6, p. 731-753, 2010. BANDE, F.; ARSHAD, S.S.; HASSAN, L.; ZAKARIA, Z.; SAPIAN, N.A.; RAHMAN, N.A.; AL-AZAWY, A. Prevalence and risk factors of feline leukaemia virus and feline immunodeficiency virus in peninsular Malaysia. BMC Veterinary Research, v. 8, n. 33, 2012. BANDECCHI, P.; DELL?OMODARME, M.; MAGI, M.; PALAMIDESSI, A.; PATRI, M.C. Feline leukaemia virus (FeLV) and feline immunodeficiency virus infections in cats in the Pisa district of Tuscany, and attempts to control FeLV infection in a colony of domestic cats by vaccination. The Veterinary Record, v. 158, p. 555-557, 2006. BANEYX, F.; MUJACIC, M. Recombinant protein folding and misfolding in Escherichia coli. Nature Biotechnology, v. 22, n. 11, p. 1399?1408, 2004. BANEYX, F. Recombinant protein expression in Escherichia coli. Current Opinion in Biotechnology, v. 10, n. 5, p. 411?421, 1999. BARBOSA, F.C.; CHRISTIANINE, M.O.T.; WALDEMARIN, K.C.A. Preval?ncia de leucemia felina em gatos dom?sticos de Uberl?ndia ? MG. Arquivo Brasileiro de Medicina Veterin?ria e Zootecnia, v. 59, n. 4, p. 939-942, 2007. BARR, F. Feline Leukemia virus. Journal of Small Animal Pratice, v. 39, n. 1, p. 41-43, 1998. BARTELING, S. J.; VREESWIJK, J. Developments in foot-and-mouth disease vaccines. Vaccine, v. 9, p. 75-88, 1991. BAYLOR, N. W.; EGAN, W.; RICHMAN, P. Aluminium salts in vaccines- US perspective. Vaccine, v. 20, n.18, 2002. BERGMAN, P.; HENDRICK, M.J.,MACY, D.; McGILL, L.; STARR, R.M.; VAN KAMPEN, K.R.Feline Sarcoma and Vaccination: Roundtable on the Injection-Site problem in cats. Veterinary Forum, v. 16, n. 3, p. 40-47, 1999. BERNARDO, A.R. Tecnologia IgY: produ??o de anticorpos avi?rios para Leishmania (Leishmania) amazonensis com o uso ?tico dos animais de experimenta??o. 2009, 49p. Disserta??o (Mestrado em Ci?ncias Veterin?rias). Universidade Federal Rural do Rio de Janeiro, Serop?dica, 2009. BIZANOV, G; JONAUSKIENA, I; HAU, F. A novel method based on lithium sulfate precipitation for purification of chicken egg yolk Immunoglobulin Y, applied to immunospecific antibodies against Sendi virus. Scandinavian Journal of Laboratory Animal Science, v. 31, p. 121-130, 2004. 56 BIZANOV, G.; NORMANTIENE, T.; JONAUSKIEN, I. Development of antibodies to Sendai virus in chickens and their isolation from yolk. Biologija, v. 2, n. 1, p. 68-71, 2006. BLANCO, K.; PRENDAS, J.; CORTES, R.; JIMENEZ, C.; DOLZ, G. Seroprevalence of Viral Infections in Domestics Cats in Costa Rice. Journal of Veterinary Medical Science. v. 71, n. 5, p. 661-663, 2009. BOLIN, L.L; LEVY, L.S. Viral Determinants of FeLV Infection and Pathogenesis: Lessons Learned from Analysis of a Natural Cohort. Viruses, v. 3, p. 1681-1698, 2010. BRADFORD, M.M. A rapid and sensitive method for the quantitation of microgram quantities utilizing the principle of protein dye binding. Analytical biochemistry, v. 72, p. 248-254, 1976. CAMPBELL, R.D.; DODDS, A.W.; PORT, R.R. The binding of human complement component C4 to antibody-antigen aggregates. The Biochemical Journal, v. 189, n. 1, p. 67-68, 1980. CARLANDER, D.; KOLLBERG, H.; WEJAKER, P.E.; LARSSON, A. Peroral immunotherapy with yolk antibodies for the prevention and treatment of enteric infections. Immunology Research, v. 21, n. 1, p. 1-6, 2000. CARROL, S.B.; THALLEY, B.S.; THEAKSTON, R.D.G. Comparison of the purity and efficacy of affinity purified avian antivenons with comercial equine crotalid antivenoms. Toxicon, v. 30, n. 9; p. 1017-1025, 1992. CHACANA, P.A.; TERZOLO, H.R.; CALAZADO, E.G.; SCHADE, R. Tecnologia IgY o aplicaciones de los anticuerpos de yema de huevo de gallina. Revista de Medicina Veterinaria, v. 85, n. 2, p. 179-189, 2004. CHALGHOUMI, R.; TH?WIS, A.; PORTETELLE, D.; BECKERS, Y. Production of hen egg yolk immunoglobulins simultaneously directed against Salmonella enteritidis and Salmonella typhimurium in the same egg yolk. Poultry Science, v. 87, n. 1, p. 32-40, 2008. CHALGHOUMI, R.; BECKERS, Y.; PORTETELLE, D.; TH?WIS, A. Hen egg yolk antibodies (IgY), production and use for passive immunization against bacterial enteric infections in chicken: a review. Biotechnology Agronomy Society and Environment, v. 13, n. 2, p.295-308, 2009. CHANG, H.M.; LU, T.C.; CHEN, C.C.; TU, Y.Y.; HWANG, J.Y. Isolation of immunoglobulin from egg yolk by anionic polysaccharides. Journal of Agricultural and Food Chemistry, v. 48, p. 995?999, 2000. CHENG, H.H.; ANDERSON, M.M.; OVERBAUGH, J. Feline leukemia virus T entry is dependent on both expression levels and specific interactions between cofactor and receptor. Virology, v. 359, p. 170-178, 2007. CHOI, J. H.; LEE, S. Y. Secretory and extracellular production of recombinant proteins using Escherichia coli. Applied Microbiology and Biotechnology, v. 64, n. 5, p. 625?635, 2004. 57 CHOU, C. P. Engineering cell physiology to enhance recombinant protein production in Escherichia coli. Applied Microbiology and Biotechnology, v. 76, n. 3, p. 521-532, 2007. COBAN, C., ISHII, K. J., GURSEL, M., KLINMAN, D. M., KUMAR, N. Effect of plasmid backbone modification by different human CpG motifs on the immunogenicity of DNA vaccine vectors. Journal of Leukocyte Biology, v. 78, n. 5, p. 647-655, 2005. COELHO, F.M.; MAIA, M.Q.; LUPPI, M.M.;COSTA, E.A.; LUIZ, A.P.M.F., RIBEIRO, N.A.; BONFIM, M.R.Q.; FONSECA, M.M.; RESENDE, M. Ocorr?ncia do v?rus da leucemia felina em Felis cattus em Belo Horizonte. Arquivo Brasileiro de Medicina Veterin?ria e Zootecnia, v. 63, n. 3, p. 778-783, 2011. COFFIN, J.M. Retroviridae: the viruses and their replication. In: FIELDS, B.N.; KNIPE, D.M.; HOWLEY, P.M.; CHANOCK, R.M.; NELMICK, J.L; MONATH, T.P.; ROZMAN, B.; STRAUS, S.E. (3ed). Fields Virology, Philadelphia: Lippincott-Raven, 1996, p. 1767-1847. COOPER, C.L.; DAVIS, H.L.; MORRIS, M.L.; EFLER, S.M.; ADHAMI, M.A.; KRIEG, A.M.; CAMERON, D.W.; HEATHCOTE, J. CpG 7909, an immunostimulatory TLR9 agonist 69 oligodeoxynucleotide, as adjuvant to Engerix-B HBV vaccine in healthy adults: a double-blind phase I/II study. Journal of Clinical Immunology. v. 24, n. 6, p. 693-701, 2004. CORNELIS, P. Expressing genes in different Escherichia coli compartments. Current Opinion in Biotechnology, v. 11, n. 5, p. 450?454, 2000. CRAWFORD, CYNDA. Progressos no diagnostico de infec??es retrovirais. In: AUGUST, J.R. Medicina Interna de Felinos. 6ed., Philadelphia, WB Saunders Company, 2011. cap. 6. p. 53-62. DAI, Y. C.; ZHANG, X.F.; TAN, M.; HUANG, P.; LEI, W.; FANG, H.; ZHONG, W.; JIANG, X. A dual chicken IgY against rotavirus and norovirus. Antiviral Research, v. 97, p. 293-300, 2012. DINGERMANN, T. Recombinant therapeutic proteins: production platforms and challenges. Biotechnology Journal, v.3, n. 1, p. 90-97, 2008. DONAHUE, P.R.; QUACKENBUSH,S.L.; GALLO, M.V.; DENORONHA, C.M.C.; OVERBAUGH, J.; HOOVER, E.A.; MULLINS, J. Viral genetic determinants of T-cell killing and immunodeficiency disease induction by the feline leukemia virus FeLV-FAIDS. Journal of Virology, v.65, p.4461-4469, 1991. DONAHUE, P.R.; HOOVER, E.A.; BELTZ, G.A.; RIEDEL, N.; HIRSCH, V.M.; OVERBAUGH, J.; MULLINS, J.I. Strong sequence conservation among horizontally transmissible, minimally pathogenic feline leukemia viruses. Journal of Virology, v. 62, n. 3, p. 722-731, 1988. DUNHAM, S.P.; GRAHAM, E. Retroviral Infections of Small Animals. Veterinary Clinics of North America: Small Animals Practice, v. 38, p. 879-901, 2008. EASTCOTT, J.W.; HOLMBERG, C.J.; DEWHIRST, F.E.; ESCH, T.R.; SMITH, D.J.; TAUBMAN, M.A. Oligonucleotide containing CpG motifs enhances immune response to 58 mucosally or systemically administered tetanus toxoid. Vaccine, v. 19, n. 13-14, p.1636-1642, 2011. EDELMAN R. Vaccine adjuvants. Reviews of Infectious Diseases, v. 2, p. 370-83, 1980. EL-MALK, M.; SHAOHONG, L.; KUMAGAI, T.; YABU, Y.; NOURELDIN, M.S.; SAUDY, N.; MARUYAMA, H.; OHTA, N. Protective Effect of Vaccination with Toxoplasma Lysate Antigen andCpG as an Adjuvant Against Toxoplasma gondii in Susceptible C57BL/6 Mice. Microbiology and Immunology, v. 49, p. 639-646, 2005. FIGUEIREDO, A.S.; ARA?JO Jr, J. P. V?rus da leucemia felina: an?lise da classifica??o da infec??o, das t?cnicas de diagn?stico e da efic?cia da vacina??o com o emprego de t?cnicas sens?veis de detec??o viral. Ci?ncia Rural, Santa Maria, v. 41, n. 11, p. 1952-1959, 2011. FLYNN, J.N., DUNHAM, S.P., WATSON, V., JARRETT, O.. Longitudinal analysis of feline leukemia virus-specific cytotoxic T lymphocytes: correlation with recovery from infection. Journal of Virology, v. 76, p. 2306- 2315, 2002. FRYER, J.; FIRCA, J.; LEVENTHAL, J.; BLONDIN, B.; MALCOLM, A.; IVANCIC, D.; GANDHI, R.; SHAH, A.; PAO, W.; ABECASSIS, M.; KAUFMAN, D.; STUART, F.; ANDERSON, B. IgY antiporcine endothelial cell antibodies effectively block human antiporcine xenoantibody binding. Xenotransplantation, v. 6, n. 2, p. 98?109, 1999. FU, C.H.; HUANG, H.; WANG, X.M.; LIU, Y.G.; WANG, Z.G.; CUI, S.J.; GAO, H.L.; LI, Z.; LI, J.P.; KONG, X.G. Preparation and evaluation of anti-SARS coronavirus IgY from yolks of immunized SPF chickens. Journal of Virology Methods. v. 133, n. 1, p. 112-115, 2006. FUJIBAYASHI, T.; NAKAMURA, M.; TOMINAGA, A.; SATOH, N.; KAWARAI, T.; NARISAWA, N. Effects of IgY against Candida albicans and Candida spp. Adherence and biofilm formation. Japanese Journal of Infections Diseases, v. 62, p. 337-342, 2009. GELLISSEN, G. Heterologous protein production in methylotrophic yeasts. Applied Microbiology and Biotechnology. v. 54, p. 741-750, 2000. GEORGIOU, G.; SEGATORI, L. Preparative expression of secreted proteins in bacteria: status report and future prospects. Current Opinion in Biotechnology, v. 16, n. 5, p.538?545, 2005. GIBERTONI, A. M.; GON?ALVES, M. C. M; MONTASSIER, M. F. S.; FERNANDES, C.C.; MONTASSIER, H. J. Clonagem, express?o e caracteriza??o da nucleoprote?na recombinante do V?rus da Bronquite Infecciosa em Escherichia coli e em Pichia pastoris. Arquivo do Instituto Biol?gico, S?o Paulo, v. 77, n.1, p.1-9, 2010. GLEICH, S.E.; KRIEGER, S.; HARTMANN K. Prevalence of feline immunodeficiency virus and feline leukaemia virus among client-owned cats and risk factors for infection in Germany. Journal of Feline Medicine and Surgery, v. 11, p. 985?992, 2009. G?MEZ, N.; SCARAMAL, J.; MIRA, G. Prevalencia de VIF y VILeF en 300 felinos en Argentina. Veterinaria Argentina, v. 16, n. 160, p. 786-793, 1999. 59 GOTTSTEIN, B.; HEMMELER, E. Egg yolk immunoglobulin Y as an alternative antibody in the serology of echinococcosis. Zeitschrift f?r Parasitenkunde, v. 71, p. 273?278, 1985. GREUNKE, K.; BRAREN, I.; ALPERS, I.; BLANK, S.; SODENKAMP, J.; BREDEHORST, R.; SPILLNER, E. Recombinant IgY for improvement of immunoglobulin-based analytical applications. Clinical Biochemistry, v. 41, n. 14-15, p. 1237-1244, 2008. GROSENBAUGH, D.A.; LEARD, T.; PARDO, M.C., MOTES-KREIMEYER, L., ROYSTON, M. Comparison of the safety and efficacy of a recombinant feline leukemia virus (FeLV) vaccine delivered transdermally and an inactivated FeLV vaccine delivered subcutaneously. Veterinary Therapeutics, v. 5, n. 4, p. 258?262, 2004. GUIMAR?ES, M.C.C.; AMARAL, L.G.; BORGES, F.V.; VIEIRA, H.P.L.; SHIMOYA, A.; MATTA, C.G.F.; MATTA, M.F.R. Production and use of egg-yolk antibody for detection of canine parvovirus in feces. Revista de Ci?ncias M?dicas e Biol?gicas, v. 7, n. 3, p. 241-248, 2008. GUPTA, R.; K, SIBER, G.R. Adjuvants for human vaccines--current status, problems and future prospects. Vaccine, v. 13, p. 1263-1276, 1995. HAGIWARA, M.K.; RECHE Jr., A; LUCAS, S.R.R. Estudo cl?nico da infec??o de felinos pelo v?rus da leucemia felina em S?o Paulo. Revista Brasileira de Ci?ncia Veterin?ria, v. 4, n .1, p. 35-38, 1997. HARDY, W.D. jr Oncogenic Virus of Cat: the leukemia and sarcoma virus. In: HOLZWORTH, J. Disease of the Cat : medicine and surgery. Philadelphia. WB Saunders Company, 1987. p. 246-268. HARDY, W.D. jr.; HIRSHAUT, Y.; HESS, P. Detection of the feline leukemia virus and other mammalian oncornaviruses by immunofluorescence. Bibliotheca Haematologica, v. 39, p. 778-799, 1973. HARDY, W.D. jr.; HESS, P.W.; MACEWEN, E.G.; MCCLELLAND, A.J.; ZUCKERMAN, E.E.; ESSEX, M.; COTTER, S. M; JARRETT, O. Biology of Feline Leukemia Virus in the Natural Environment. Cancer Research, n. 36, p. 582-588, 1976a. HARDY, W.D. jr.; McCLELLAND, A. J.; ZUCKERMAN, E. E.; HESS, P. W.; ESSEX, M.; COTTER, S. M.; C. ; HAYES, A. A. Prevention of the contagious spread of feline leukaemia virus and the development of leukaemia in pet cats. Nature, v. 263, p. 326-328, 1976b. HARTMANN, K. Feline Leukemia Virus Infection. In: GREENE, C. E. Infectious Diseases of the Dog and Cat. 3ed., Philadelphia, WB Saunders Company, 2006. cap.13. p. 105-130. HARTMANN, K.; GRIESSMAYR, P.; SCHULZ, B.; GREENE, C.E.; VIDYASHANKAR, A. N.; JARRETT, O. ; EGBERINK, H. F. Quality of different in-clinic test systems for feline immunodeficiency virus and feline leukaemia virus infection. Journal of Feline Medicine and Surgery, v. 9, p. 439-445, 2007. HATTA, H.; TSUDA, K.; AKACHI, S.; KIM, M.; YAMAMOTO, T. Productivity and some properties of egg yolk antibody (IgY) against human rotavirus compared with rabbit IgG. Bioscience, Biotechnology and Biochemistry, v. 57, n. 3, p. 450-454, 1993. 60 HELFER-HUNGERBUEHLER, K.; CATTORI, V.; BORETTI, F.S.; OSSENT, P., GREST, P.; REINACHER, M.; HENRICH, M.; BAUER, E., BAUER-PHAM, K.; NIEDERER, E.; HOLZNAGEL, E.; LUTZ, H.; HOFMANN-LEHMANN, R. Dominance of highly divergent feline leukemia virus A progeny variants in a cat with recurrent viremia and fatal lymphoma, Retrovirology, v. 7, n. 14, p. 1-14, 2010. HEMMI, H.; TAKEUCHI, O.; KAWAI, T.; KAISHO, T.; SATO, S.; SANJO, H.; MATSUMOTO, M.; HOSHINO, K.; WAGNER, H.; TAKEDA, K.; AKIRA, S. A Toll-like receptor recognizes bacterial DNA. Nature, v. 408, p.740?745, 2000. HERRING, E.S; TROY, G.C.; TOTH, T.E; FORRESTER, S.D.; WEIGT, L.A.; HERRING, I.P. Detection of feline leukaemia virus in blood and bone marrow of cats with varying suspicion of latent infection. Journal of Feline Medicine and Surgery, v. 3, n. 3, p. 133-141, 2001. HILLEMAN, M. R. Critical appraisal of emulsified oil adjuvants applied to viral vaccine. Progress in Medical Virology, v. 8, p. 131-182, 1966. HIRAI, K.; ARIMITSU, H.; UMEDA, K.; YOKOTA, K.; SHEN, L.; AYADA, K. Passive oral immunization by egg yolk immunoglobulin (IgY) to Vibrio cholerae effectively prevents cholera. Acta Medica Okayama. v. 64, p. 163-170, 2010. HOFMANN-LEHMANN, R.; VALENTINO CATTORI, V.; RAVI TANDON, R.; BORETTI, F.S.; MELI, M.L.; RIOND, B.; LUTZ, H. How molecular methods change our views of FeLV infection and vaccination. Veterinary Immunology and Immunopathology, v. 123, p. 119?123, 2008. HOOVER, E.A.; MULLINS, J. I. Feline leukemia virus infection and diseases. Journal of American Veterinary Medical Association, v. 199, p.1287-1297, 1991. HORIKOSHI, T.; HIRAOKA, J.; SAITO, M.; HAMADA, S. IgY antibody from hen egg yolks: purification by ethanol fractionation. Journal of Food Science, v. 58, n. 4, p. 739-742, 1993. HUOPALAHTI, R. et al. 2007. In: Bioactive Egg Compounds. Dieter Czeschlik (ed.), Springer-Verlag Berlin Heidelberg. 297 p. ISMAIL, A. A. A radical approach is needed to eliminate interference from endogenous antibodies in immunoassays. Clinical Chemistry, v. 51, n. 1, p. 25-26, 2005. JANA, S.; DEB, J. K.; Strategies for efficient production of heterologous proteins in Escherichia coli. Applied Microbiology and Biotechnology, v. 67, n. 3, p. 289-98, 2005. JARRETT, O.; LAIRD, H.M.; HAY, D. Determinants of the host range of feline leukemia viruses. Journal of General Virology, v. 20, n. 2, p. 169-175, 1973. JARRETT, O. Strategies of retrovirus survival in the cat. Veterinary Microbiology, v. 69, p. 99-107, 1999. JIN, W.; YAMADA, K.; IKAMI, M.; KAJI, N.; TOKESHI, M.; ATSUMI, Y.; MIZUTANI, M.; MURAI, A.; OKAMOTO, A.; NAMIKAWA, T.; BABA, Y.; OHTA, M. Application of 61 IgY to sandwich enzyme-linked immunosorbent assays, lateral flow devices, and immunopillar chips for detecting staphylococcal enterotoxins in milk and dairy products. Journal of Microbiological Methods, 2013. No prelo. JONASSON, P.; LILJEQVIST, S.; NYGREN, P. A.; STAHL, S. Genetic design for facilitated production and recovery of recombinant proteins in Escherichia coli. Biotechnology Applied Biochemistry, v. 35, n. 2, p.91?105, 2002. JUNQUEIRA-JORGE, J.; FERREIRA, F.; HAGIWARA, M.K. Risk factors for feline leukemia virus (FeLV) infection in cats in S?o Paulo, Brazil. Brazilian Journal Veterinary Research Animal Science, v. 48, n. 5, p. 392-398, 2011. KADIOGLU, A.; COWARD, W.; COLSTON, M.J.; HEWITT, C.R.A.; ANDREW, P.W.; CD4-T-Lymphocyte Interactions with Pneumolysin and Pneumococci Suggest a Crucial Protective Role in the Host Response to Pneumococcal Infection. Infection and Immunity, v. 72, n. 5, p. 2689-97, 2004. KANO, F.S.; VIDOTTO, O.; VIDOTTO, M.C. Vacina de DNA: aspectos gerais e sua aplica??o na medicina humana e veterin?ria. Ci?ncias Agr?rias, v. 28, n. 4, p. 709-726, 2007. KAPOOR, P.; COMPTON, M.M.; HOWARTH, B. Immunization of chickens with quail and turkey perivitelline membrane proteins: production of antibodies and their effects on fertility. Poultry Science, v. 79, n. 2, p. 245-256, 2000. KLEMPERER F. Ueber nat?rliche lmmunit?t und ihre Verwerthung f?r die Immunisirungstherapie. Archiv f?r Experimentelle Pathologie und Pharmakologie v.31, p. 356-382, 1883 apud Narat M. Production of antibodies in chickens. Food Technology Biotechnology v. 41, n.3, p. 259-267, 2003. KLINMAN, D.M.; AE-KYUNG, Y.; BEAUCAGE, S.L.; CONOVER, J.; KRIEG, A.M. CpG Motifs Present in Bacterial DNA Rapidly Induce lymphocytes to Secrete Interleukin 6, Interleukin 12, and Interferon Gamma. Proceedings of the National Academy of Sciences of the United States of America, v. 93, p. 2879-2883, 1996. KLINMAN, D.M.; BARNHART, K.M.; CONOVER, J. CpG motifs as immune adjuvants. Vaccine. v.17, p.19-25, 1999. KNOTEK, Z.; HADJOKOVA, P.; SVOBODA, M.; TOMAN, M.; RASKA, V. Epidemiology of feline leukaemia and feline immunodeficiency virus in Czech Republic. Journal of Veterinary Medicine Series, v. 26, n. 10, p. 665-671, 1999. KOLLBERG, H.; CARLANDER D.; OLESEN H.; WEJAKER, P.E.; JOHANNESSON, M.; LARSSON, A. Oral administration of specific yoIk antibodies (IgY) may prevent Pseudomonas aeruginosa infections in patients with cystic fibrosis: a phase I feasibility study. Pediatric Pulmonology, v. 35, p. 433-440, 2003. KRIEG, A.M.; AE-KYUNG, Y.; MATSON, S.; WALDSCHMIDT, T.J.; BISHOP, G.A.; TEASDALE, R.; KORETZKY, G.A.; KLINMAN, D.M. CpG Motifs in Bacterial DNA Trigger Direct B-cell Activation. Nature, v. 374, p. 546-549, 1995. 62 KURLAND, C.; GALLANT, J.; Errors of heterologous protein expression. Current Opinion in Biotechnology, v. 7, n. 5, p. 489-93, 1996. LAPPIN, M.R. Feline Leukemia Virus. In: Semin?rio Internacional de Doen?as Infecciosas, 1998 , S?o Paulo. Anais...p. 36-44. LARENTIS, A. L.; ARGONDIZZO, A.P.C.; ESTEVES, G.S.; JESSOURON, E.; GALLER, R.; MEDEIROS, M.A. Cloning and optimization of induction conditions for mature PsaA (pneumococcal surface adhesin A) expression in Escherichia coli and recombinant protein stability during long-term storage. Protein Expression and Purification, v. 78, n. 1, p. 38-47, 2011. LARSSON, A.; KARLSSON-PARRA, A.; SJOQUIST, J. Use of chicken antibodies in enzyme immunoassays to avoid interference by rheumatoid factors. Clinical Chemistry, v. 37, p. 411-414, 1991. LEE, E.N.; SUNWOO, H.H.; MENNINEN, K.; SIM, J.S. In vitro studies of chicken egg yolk antibody (IgY) against Salmonella enteritidis and Salmonella typhimurium. Poultry Science, v. 81, p. 632-641, 2002. LEE, S. Y.; CHOI, J. H.; XU, Z. Microbial cell-surface display. Trends in Biotechnology, v. 21, n. 1, p. 45?52, 2003. LEV?SQUE, S.; MARTINEZ, G.; FAIRBROTHER, J.M. Improvement of adjuvant systems to obtain a cost-effective production of high levels of specific IgY. Poultry Science, v.86, p. 630?635, 2007. LEVY, J.; CRAWFORD, P. C.; HARTMANN, K.; HOFMANN-LEHMANN, R.; LITTLE, S.; SUNDAHL, E. American Association of Feline Practitioners? feline retrovirus management guidelines. Journal of Feline and Surgery, v. 10, n .3, p.300-316, 2008. LEVY, J. K.; SCOTT, H. M.; LACHTARA, J. L.; CRAWFORD, P. C. Seroprevalence of feline leukemia virus and feline immunodeficiency virus infection among cats in North America and risk factors for seropositivity. Journal of the American Veterinary Medical Association, v. 228, n .3, p. 371-376, 2006. LIN, J.A.; CHENG, M.C.; INOSHIMA, Y; TOMONAGA, K.; MIYAZAWA, T.; TOHYA, Y.; TOH, K.; LU, Y.S.; MIKAMI, T. Seroepidemiological survey of feline retrovirus infections in cats in Taiwan in 1993 and 1994. Journal of Veterinary Medical Science, v. 57, n. 1, p. 161-163, 1995. LINDAHL, T.L.; FESTIN, R.; LARSSON, A. Studies of fibrinogen binding to platelets by flow cytometry: An improved method for detection of platelet activation. Thrombosis and Haemostasis, v. 68, p. 221-225, 1992. LINDBLAD, E. Aluminium adjuvants. In: Stewart- Tull, D. Editor. The theory and practical application of adjuvants. New York: Wiley, p. 21- 35, 1995. LINENBERGER, M.L.; ABKOWITZ, J.L. Haematological disorders associated with feline retrovirus infections. Bailli?re's clinical haematology, v. 8, n. 1, p. 73-112, 1995. 63 LIOU, J.F; CHANG, C.W.; TAILIU, J.J.; YU, C.K.; LEI, H.Y.; CHEN, L.R. Passive protection effect of chicken egg yolk immunoglobulins on enterovirus 71 infected mice. Vaccine, v. 28, p. 8189-8196, 2010. LITTLE, S.; SEARS, W.; LACHTARA, J.; BIENZLE, D. Seroprevalence of feline leukemia virus and feline immunodeficiency virus infection among cats in Canada. The Canadian Veterinary Journal, v. 50, p. 644?648, 2009. LOAR, A.S. The controversy continues. In: THE FIFTH ANNUAL VETERINARY MEDICAL FORUM, 15, 1987. Proceedings?American College of Veterinary Internal Medicine. 1987. p. 338-342. LOAR, A.S. Feline Leukemia virus: Imunization and Prevention. Veterinary Clinics of North America: Small Animal Practice, v.23, n.1, p. 193-211, 1993. LOSSO, J. N.; KUMMER, A.; LI-CHAN, E.; NAKAI, S. Development of a particle concentration fluorescence immunoassay for the quantitative determination of IgG in bovine milk. Journal of agricultural and food chemistry, v. 41, p. 682-686, 1993. LUCAS, S.R.R; HAGIWARA, M.K.; RECHE JR., A.; GERMANO, P.M.L. Ocorr?ncia de anticorpos antitoxoplasma em gatos infectados naturalmente pelo v?rus da imunodefici?ncia dos felinos. Brazilian Journal of Veterinary Research and Animal Science, v. 35, n. 1, p. 41-45, 1998. LUCHESE, M.D.; ARGONDIZZO, A.P.C.; JESSOUROUN, E.; PEREIRA, D.S.G.; ANDRADEGOES, A.C.M.; ARDENGHI, P.G.; MEDEIROS, M.A.; LARENTIS, A.L. Influ?ncia de Diferentes Condi??es na Express?o da Prote?na Recombinante Pneumolisina (rPly). Anais do XVII Simp?sio Nacional de Bioprocessos. Sinaferm, 2009. LUTZ, H; ADDIE, D; BELAK, S. et al. Feline leukaemia ABCD guidelines on prevention and management. Journal of Feline Medicine and Surgery, v.11, p. 565-74, 2009. MACIEIRA, D.B.; MENEZES, R.C..A.A.; DAMICO, C.B; ALMOSNY, N.R.P., MCLANE, H.L.; DAGGY, J.K.; MESSICK, J.B. Prevalence and risk factors for hemoplasmas in domestic cats naturally infected with feline immunodeficiency virus and/or feline leukemia virus in Rio de Janeiro - Brazil. Journal of Feline Medicine and Surgery, v. 10, p. 120-129, 2008. MACY, D.W.; HENDRICK, M.J. The potential role of inflammation in the development of postvaccinal sarcomas in cats. Veterinary Clinics of North America: Small Animal Practice, v. 26 , n. 1 , p. 103-109, 1996. MCLAREN, R.D.; PROSSER, C.G.; GRIEVE, R.C.J. The use of caprylic acid for extraction of immunoflobulin fraction from egg yolk of chickens immunized with ovine ?-lactalbumin. Journal of Immunological Methods, v. 177, n. 1, p. 175-184, 1994. MADEWELL, B.R.; JARRET, O. Recovery of feline leukaemia virus from nonviraemic cats. Veterinary Record, v. 112, n. 1, p. 90-93, 1983. MAHMOOD, M.S.; SIDDIQUE, M.; HUSSAIN, I.; KHAN, A.; MANSOOR, M.K. Protection capability of recombinant plasmid DNA vaccine containing VP2 gene of very 64 virulent infectious bursal disease virus in chickens adjuvanted with CpG oligodeoxynucleotide. Vaccine, v. 24, n. 22, p. 4838-4846, 2006. MAKRIDES, S. C. Strategies for achieving high-level expression of genes in Escherichia coli. Microbiological Reviews, v. 60, n. 3, p. 521-538, 1996. MARCIANI, D.J.; KENSIL, C.R.; BELTZ, G.A.; HUNG, C.H.; CRONIER, J.; AUBERT, A. Genetically-engineered subunit vaccine against feline leukaemia virus: protective immune response in cats. Vaccine, v. 9, 1991. MAZUR, C.; REIS, J.K.P.; LEITE, R.C.; DANELLI, M.G.M.; HAGIWARA, M.K.; G?ES, A.C.M.A.; MEDEIROS, M.A. Evaluation of a recombinant p24 antigen for the detection of Feline Immunodeficiency Virus-specific antibodies. Pesquisa Veterin?ria Brasileira, v. 30; n. 10, p. 877-880, 2010. MEHL, M.L. Feline Leukemia virus. In: LAPPIN, M.R. Feline International Medicine Secrets, Philadelphia: Hanley & Belfus. 2001. cap.76. p. 387-391. MEINERZ, A.R.M.; ANTUNES, T.A.; SOUZA, L.L.; NASCENTE, P.S.; FARIA, R.O.; CLEFF, M.B.; GOMES, F.R.; NOBRE, M. S.O; REISCHAK, D.; SCHUCH, L.F.D.; MEIRELES, M.C.A. Frequ?ncia do v?rus da leucemia felina (vlfe) em felinos dom?sticos (felis catus) semidomiciliados nos munic?pios de Pelotas e Rio Grande. Ci?ncia Animal Brasileira, v. 11, n. 1, p. 90-93, 2010. MENDOZA, R.; ANDERSON, M.M.; OVERBAUGH, J. A Putative Thiamine Transport Protein Is a Receptor for Feline Leukemia Virus Subgroup A. Journal of Virology, v. 80, p. 3378?3385, 2006. MEULENAER, B.; HUYGHEBAERT, A. Isolation and purification of chicken egg yolk immunoglobulins: a review. Food and Agricultural Immunology, v. 13, n. 1, p. 275- 288, 2001. MINE, Y.; KOVACS-NOLAN, J. Chicken egg yolk antibodies as therapeutics in enteric infectious disease: a review. Journal of Medical Food, v. 5, n. 3, p. 159-169, 2002. MIYAZAWA, T. Infections of feline leukemia virus and feline immunodeficiency virus. Front Biosci, v. 7, p. 504-518, 2002. MOORE, A.S; OGILVIE, G.K. Lymphoma in Feline Oncology: A Comprehensive Guide to Compassionate Care. Veterinary Learning Systems, p. 191-219, 2001. MOORE, G. E.; WARD, M. P.; DHARIWAL, J. Use of a primary care veterinary medical database for surveillance of syndromes and diseases in dogs and cats. Journal of Veterinary Internal Medicine, v. 18, p. 386, 2004. MOREIRA, A.S.A.S. Produ??o antiveneno botr?pico em ovos de galinha. 2007. 48 p. Disserta??o (Mestrado em Ci?ncia Animal) - Universidade Federal de Minas Gerais, Belo Horizonte, 2007. 65 MORRISON, S.L.; MOHAMMED, M.S.; WIMS, L.A.; TRINH, R.; ETCHES, R. Sequences in antibody molecules important for receptor-mediated transport into the chicken egg yolk. Molecular Immunology, v. 38, n. 8, p.619-625, 2001. MOTOI, Y.; INOUE, S.; HATTA, H.; SATO, K.; MORIMOTO, K.; YAMADA, A.; Detection of rabies-specific antigens by egg yolk antibody (IgY) to the recombinant rabies v?rus proteins produced in Escherichia coli. Japanese Journal of Infectious Diseases, v. 58, p. 115-118, 2005. NARAT M. Production of antibodies in chickens. Food Technology and Biotechnology, v. 41, n.3, p. 259-267, 2003. NORSWORTHY, G.D. Feline leukemia virus diseases. In: NORSWORTHY, G.D. Feline Practice. Philadelphia: J.B. Lippincott Company. 1993. p. 360-368. OLIVEIRA, A.P. Express?o da prote?na s1 recombinante do v?rus da bronquite infecciosa em Saccharomyces cerevisiae para aplica??o no imunodiagn?stico. 2008, 54p. Disserta??o (Mestrado em Microbiologia). Universidade Estadual Paulista, Faculdade de Ci?ncias Agr?rias e Veterin?rias, Jaboticabal, S?o Paulo, 2008. OLOVSSON, M.; LARSSON, A. Biotin labelling of chicken antibodies and their subsequent use in ELISA and immunohistochemistry. Comparative Immunology, Microbiology and Infectious Diseases, v. 16, n. 2, p. 145-152, 1993. OVERBAUGH, J. Molecular cloning of feline leukemia virus that induces fatal immunodeficiency disease in cats. Science, v. 239, n. 19, p. 906-910, 1988. OVERBAUGH, J.; BANGHAM, C.R.M. Selection forces and constraints on retroviral sequence variation. Science, v. 292, p. 1106-1109, 2001. PACITTI, A. M.; JARRETT, O. Duration of the latent state in feline leukaemia virus infections. Veterinary Record, v. 117, p. 472-474, 1985. PACITTI, A.M.; JARRETT, O.; HAY, D. Transmission of feline leukaemia virus in the milk of a non-viraemic cat. Veterinary Record, v. 118, p. 381-384, 1986. PARMA, Y.R.; CHACANA, P.A.; ROG?, A.; KAHL, A.; CANGELOSI, A.; GEOGHEGAN, P.; LUCCHESI, P.M.A.; FERN?NDEZ-MIYAKAWA, M.E. Antibodies anti-Shiga toxin 2 B subunit from chicken egg yolk: Isolation, purification and neutralization efficacy. Toxicon, v. 58 p. 380?388, 2011. PAULY, D.; DORNER, M.; ZHANG, X.; HLINAK, A.; DORNER, B.; SCHADE, R. Monitoring of laying capacity, immunoglobulin Y concentration, and antibody titer development in chickens immunized with ricin and botulinum toxins over a two-year period. Poultry Science, v.88, n.2, p. 281-290, 2009. PEDERSEN, N. C.; MERIC, S.M.; JOHNSON, L.; PLUCKER,S.; THEILEN, G.H. The clinical significance of latent feline leukemia virus infection in cats. Feline Pratice, v. 14, p. 32-48, 1984. 66 PLOTKIN, S.A., ORENSTEIN, W.A. Vaccines. 4th ed. Saunders. Philadelphia, 2004. p.69-79. POLSON, A.; VON WECHMAR, M.B.; VAN REGENMORTEL, M.H. Isolation of viral IgY antibodies from yolks of immunized hens. Immunological Communications, v. 9, n. 5, p. 475-93, 1980. POULET, H.; BRUNET, S.; BOULARAND, C.;. GUIOT, A.L.; LEROY, V.; TARTAGLIA, J.; MINKE, J.; AUDONNET, J.C.; DESMETTRE, R. Efficacy of a canarypox virus-vectored vaccine against feline leukaemia. Veterinary Record, v. 153, p. 141-145, 2003. QUIGLEY, J.G., BURNS, C.C., ANDERSON, M.M., LYNCH, E.D., SABO, K.M., OVERBAUGH, J., ABKOWITZ, J.L. Cloning of the cellular receptor for feline leukemia virus subgroup C (FeLV-C), a retrovirus that induces red cell aplasia. Blood, v. 95, n. 3, p. 1093?1099, 2000. RANGEL, M.F.N.; LEMOS, L.S.; ALMEIDA, C.M.C.; SALES, L.G.; VIEIRA-DA-MOTTA. O. Development of IgY antibodies in chickens and IgG in rabbits immunized against proteins of Pythium insidiosum isolated from horses in the state of Rio de Janeiro. Pesquisa Veterin?ria Brasileira, v. 30, n. 1, p. 87-93, 2010. RESENDE, F.C.B.; PASSOLD, J.; FERREIRA, S.I.A.C.; ZANETTI, C.R.; LIMA, H.C. Adjuvantes de vacinas: possibilidades de uso em seres humanos ou animais. Revista brasileira de alergia e imunopatologia, v. 27, n. 3, 2004. RICHARDS, J.R.; ELSTON, T.H.; FORD, R.B. The 2006 American Association of Feline Practitioners Feline Vaccine Advisory Panel report. Journal of the American Veterinary Medical Association, v. 229, n.9, p.1405?1441, 2006. ROJKO, J.L.; HARDY Jr., W.D. Feline Leukaemia virus and other retroviruses. In: SHERDING, R.G. The Cat: diseases and clinical management. New York. Churchill Livingstone, 1994, 2.ed, v. 1, p. 263-432. ROJKO, J. L.; KOCIBA, G.J. Pathogenesis of infection by the feline leukemia virus. Journal of American Veterinary Medical Association, v. 25, n. 4, p. 6-11, 1991. ROSE, M.E.; ORLANS, E.; BUTTRESS, N. Immunoglobulin classes on the hen?s egg: their segregation in yolk and white. European Journal Immunology. v. 4, p. 521?523, 1974. ROSOL, T.J; STEINMEYER, C.L.; MCCAULEY, L.K.; MERRYMAN, J.I; WERKMEISTER, J.R; GRONE, A.; WECKMANN, M.T.; SWAYNE, D.E.; CAPEN, C.C. Studies on chicken polyclonal anti-peptide antibodies specific for parathyroid hormone-related protein (I-36). Veterinary Immunology and Immunopathology, v. 35, p. 321-337, 1993. SAMBROOK, J.; RUSSEL, D.W. Molecular Cloning: A Laboratory Manual. 3. ed. New York, USA: Cold Spring Harbor Laboratory Press, 2001. 2344 p. SANGER, F.; NICKLEN, S.; COULSON, A.R. DNA sequencing with chain-terminating inhibitors, Proceedings of National Academy of Sciences of United States of America, v. 74, n.12, p. 5463-5467, 1977. 67 SCHADE, R. IgY-antibodies, future prospects. In: SCHADE, R.; BEHN, I.; ERHARD, M. HLINAK, A.; STAAK, C. Chicken egg yolk antibodies, production and application. IgY-technology. Berlim: Springer, 2001. p. 218-222. SCHADE, R.; CALZADO, E.G.; SARMIENTO, R., CHACANA, P.A.; PORANKIEWICZ-ASPLUND, J.; TERZOLO, H.T. Chicken Egg Yolk Antibodies (IgY-tecnology): A review of Progress in production and Use in Research and Human and Veterinary Medicine. Alternatives of Laboratory Animals, v. 33, p. 1-26, 2005. SCHMIDT, P., ERHARD, M.H.; SCHAMS, D.; HAFNER, A.; FOLGER, S.; LOSCH, U. Chicken egg antibodies for immunohistochemical labelling of growth-hormone and prolactin in bovine pituitary gland. The Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry, v. 41, p. 1441-1446, 1993. SCHUBACH, T.M.P.; SCHUBACH, A.O.; OKAMOTO, T.; FIGUEIREDO, F.B.; PEREIRA, S.A.; SANTOS, I.B.; REIS, R.S.; PAES, R.A.; PEREZ, M.C.A.; MARZOCH, M.C.A; FRANCESCONI-DO-VALLE, A.C.; WANKE, B. Utilidade do co?gulo sang??neo para o isolamento de Sporothrix schenckii de gatos naturalmente infectados. Brazilian Journal of Veterinary Research and Animal Science, v. 41, p. 404-408 , 2004 SHAHBAZI, P.; SHAYAN, P.; EBRAHIMZADEH, E.; RAHBARI, S. Specific Egg Yolk Antibody against Recombinant Cryptosporidium parvum P23 Protein. Iranian Journal of Parasitology. v.4, n.3, p. 15-24, 2009 SHELTON, G.H.; WALTIER, R.M.; CONNOR, S.C.; GRANT, C.K. Prevalence of immunodeficiency v?rus and leukemia virus infection in pet cats. Journal of the American Animal Hospital Association, v. 25, p. 7-12, 1989. SHIMIZU, M.; NAKAI, S.; FITZSIMMONS, R.C. Anti-E. coli immunoglobulin Y isolated from egg yolk of immunized chickens as a potential food ingredient. Journal of Food Science, v. 53, n. 5, p. 1360-1366, 1998. SHIMIZU, M.; NAGASHIMA, H.; SANO, K.; HASHIMOTO, K.; OZEKI, M.; TSUDA, K.; HATTA, H. Molecular stability of chicken and rabbit immunoglobulin G. Bioscience, Biotechnology, and Biochemistry, v. 56, n. 2, p. 270-274, 1992. SHIMIZU, M.; NAGASHIMA, H.; HASHIMOTO, K. Comparative studies in molecular stability of immunoglobulin G from different species. Comparative Biochemistry and hysiology ? Part: B: Biochemistry and Molecular Biology, v. 106, n. 2, p. 255-261, 1993. SHIMIZU, M.; NAGASHIMA, H.; HASHIMOTO, K.; SUZUKI, T. Egg yolk antibody (IgY) stability in aqueous solution with high sugar concentrations. Journal of Food Science, v. 59, n. 4, p. 763-772, 1994. SHIN, J.H.; YANG, M.; NAM, S.W.; KIM, J.T.; MYUNG, N.H.; BANG, W.G.; ROE, I.H. Use of egg yolk-derived immunoglobulin as an alternative to antibiotic treatment for control of Helicobacter pylori infection. Clinical and Diagnostic Laboratory Immunology. v. 9, n. 5, p. 1061-1066, 2002. 68 SILVA, F. R. C. Preval?ncia das infec??es pelo v?rus da leucemia viral felina e da imunodefici?ncia viral felina na cidade de Porto Alegre. 2007, 57p. Disserta??o (Mestrado em Ci?ncias Veterin?rias). Universidade do Rio Grande do Sul, Porto Alegre, 2007. SILVA, J.M. C?digo gen?tico. In: Bioqu?mica da Informa??o Gen?tica, 2006. Dispon?vel em: http://hdl.handle.net/10451/1120 SILVA, W. D.; TAMBOURGI, D.V. IgY: A promising antibody for use in immunodiagnostic and in immunotherapy. Veterinary Immunology and Immunopathology, v. 135, n. 3-4, p. 173-180, 2010. SINGH, M.; BRIONES, M.; OTT, G.; O?HAGAN, D. Cationic microparticles: a potent delivery system for DNA vaccines. Proceedings of the National Academy of Sciences of the United States of America, v. 97, p. 811?816, 2000. SINGH, M.; O?HAGAN, D. T. Recent advances in vaccine adjuvants. Pharmaceutical Research, v. 19, n. 6, p.715-728, 2002. SMITH, C.A. Are we vaccinating too much? Journal of the American Veterinary Medical Association, v. 207, n. 4, p. 421-425, 1995. SMITH, D.J; KING, W.F.; GODISKA, R. Passive transfer of immunoglobulin Y antibody to Streptococcus mutans glucan binding protein B can confer protection against experimental dental caries. Infection and Immunity, v. 69, n. 3, p.135-142, 2001. SORENSEN, H. P.; MORTENSEN, K. K. Advanced genetic strategies for recombinant protein expression in Escherichia coli. Journal of Biotechnology, v.115, p.113-128, 2005(a). SORENSEN, H. P.; MORTENSEN, K. K. Soluble expression of recombinant proteins in the cytoplasm of Escherichia coli. Microbial Cell Factories, v. 4, n. 1, p. 1-8, 2005(b). SOTIROPOULOU, G.; PAMPALAKIS , G.; PROSNIKLI, E.; EVANGELATOS, G.P.; LIVANIOU, E. Development and immunochemical evaluation of a novel chicken IgY antibodyspecific for KLK6. Chemistry Central Journal, v. 6, n. 148, 2012. SOUZA, L.L.; NOBRE, M.O.; SILVEIRA, E; DILMARA REICHAK, D.; NASCENTE, P. Esporotricose em gatos portadores do v?rus da leucemia felina. Revista Brasileira de Ci?ncia Veterin?ria. v.12, n.1/3, p.99-101, 2005. SOUZA, H.J.M.; TEIXEIRA, C.H.R.; GRA?A, R.F.S. Estudo epidemiol?gico de infec??es pelo v?rus da leucemia e/ou imunodefici?ncia felina, em gatos dom?sticos do munic?pio do Rio de Janeiro. Cl?nica Veterin?ria, n. 36, p. 14-21, 2002. SOUZA, H.J.M.; TEIXEIRA, C.H.R. In: SOUZA, H.J.M. Leucemia viral felina In: Colet?neas em medicina e cirurgia felina. Rio de Janeiro: L.F. Livros de Veterin?ria, cap.22, p. 251-271, 2003. SPARKES, A.H. Feline leukaemia virus: a review of immunity and vaccination. Journal of Small Animal Practice , v. 38, n. 5, p. 187-194, 1997. 69 SPARKES, A.H. Feline leukaemia virus and vaccination. Journal of Feline Medicine and Surgery, v. 5, p. 97-100, 2003. STAAK, C. Egg Yolk Antibodies (IgY) in Routine Diagnostic Work. Altex., v. 13, n.5, p.73-75, 1996. STEWART, H.; JARRETT, O.; HOSIE, M. J.; WILLETT, B. J. Are endogenous feline leukemia viruses really endogenous? Veterinary immunology and immunopathology, v.15, n.143 (3-4), p.325-31, 2011. SPILLNER, E.; BRAREN, I.; GREUNKE, K.; SEISMANN, H.; BLANK, S.; DU PLESSIS, D. Avian IgY antibodies and their recombinant equivalents in research, diagnostics and therapy, Biologicals, v. 40, p. 313-322, 2012. STRACHAN, T.; READ, A.P. Human Molecular Genetics. 2nd edition. New York: Wiley-Liss; 1999. Chapter 9, Instability of the human genome: mutation and DNA repair. Dispon?vel em: http://www.ncbi.nlm.nih.gov/books/NBK7566/ SUI, J.; CAO, L.; LIN, H. Antibacterial activity of egg yolk antibody (IgY) against Listeria monocytogenes and preliminary evaluation of its potential for food preservation. Journal of the Science of Food and Agriculture. v. 91, p. 1946-1950, 2011. SUNTZ, M.; FAILING, K.; HECHT, W; SCHWARTZ, D.; REINACHER, M. High prevalence of non-productive FeLV infection in necropsied cats and significant association with pathological findings. Veterinary Immunology and Immunopathology, v. 136, p. 71?80, 2010. SVENDSEN, B.L.; COWLEY, A.; OSTERGAARD, L.H. Development and comparison of purification strategies for chicken antibodies from egg yolk. Laboratory Animal Science, v.45, n.1, p.89-93, 1995. TENNANT, B. Feline injection-site fibrosarcomas: results of a BSAVA survey. Journal of Small Animal Pratice, v. 41, n. 4, p. 181-182, 2000. TERPE, K. Overview of bacterial expression systems for heterologous protein production: from molecular and biochemical fundamentals to commercial systems. Applied Microbiology and Biotechnology, v. 72, n. 2, p.211?222, 2006. TORRES, A.T; MATHIASON, C.K; HOOVER, E.A. Re-examination of feline leukemia virus: host relationships using real-time PCR. Virology, v. 332: p. 272?83, 2005. TSUKAMOTO M, HIROI S, ADACHI K, KATO H, INAI M, KONISHI I. Antibodies against swine influenza virus neutralize the pandemic influenza virus A/H1N1. Molecular Medicin Report. v.4, p.209-214, 2010. VASCONCELOS, G.A.L.B.M. Produ??o de anticorpos IgY espec?ficos para o v?rus da hepatite A purificados de gema de ovo de frangas imunizadas e sua poss?vel aplica??o em diagn?stico do v?rus no f?gado. 104p. 2010. Disserta??o (mestrado em Ci?ncias) ? Instituto Oswaldo Cruz, P?s-gradua??o em Biologia Parasit?ria, 2010. 70 VERDOLIVA, A.; BASILE, G.; FASSINA, G. Affinity purification of immunoglobulins from chicken egg yolk using a new synthetic ligand. Journal of Chromatography B: Biomedical Science and Applied, v. 749, n. 2, p. 233-242, 2000. WARR, G.W.; MAGOR, K.E.; HIGGINS, D.A. IgY: clues to the origins of modern antibodies. Immunology Today, v. 16, n. 8, p. 392-398, 1995. WEICKERT, M.J., et.al. Optimization of heterologous protein production in Escherichia coli Current Opinion of Biotechnology, v.7, p. 494-499, 1996. WEISS, A. T. H.; KLOPFLEISCH, R.; GRUBER, D.A. Prevalence of feline leukaemia provirus DNA in feline lymphomas. Journal of Feline Medicine and Surgery v. 12, p. 929-935, 2010. WILLETT, B.; HOLSIE, M.J. Feline leukaemia virus: Half a century since its discovery. The Veterinary Journal , v. 195, n. 1, p. 16-23, 2012. WOOLLEY, A.; LANDON, J. Journal of Immunological Methods, v. 178, p. 253-265, 1995. WU, F.; YUAN, X.Y.; LI, J.; CHEN, Y.H. The co-administration of CpG-ODN influenced protective activity of influenza M2e vaccine. Vaccine, v. 27, n. 32, p. 4320-4324, 2009. ZHANG, Y.; PALMER, G. H.; ABBOTT, J. R.; HOWARD, C. J.; HOPE, J. C.; BROWN, W. C. CpG ODN 2006 and IL-12 are comparable for priming Th1 lymphocyte and IgG responses in cattle immunized with a rickettsial outer membrane protein in alum. Vaccine, v. 21, n. 23, p. 3307-3318, 2003. ZHEN, Y.H.;JIN, L.J.; LI, X.Y.; GUO, J.; LI, Z.; ZHANG, B.J. FANG, R.; XU, Y.P. Efficacy of specific egg yolk immunoglobulin (IgY) to bovine mastitis caused by Staphylococcus aureus. Veterinary Microbiology, v. 133, p. 317?322, 2009.FeLVimunodiagn?sticoIgYimmunodiagnosisMedicina Veterin?riaClonagem e express?o da prote?na recombinante p27 (r-p27) do v?rus da leucemia felina e produ??o de IgY avi?ria contra a r-p27 para utiliza??o em imunodiagn?sticoCloning and expression of p27 protein (r-p27) of Feline Leukemia Virus and avian IgY production against the r-p27 to immunodiagnostic applicationinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisinfo:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UFRRJinstname:Universidade Federal Rural do Rio de Janeiro (UFRRJ)instacron:UFRRJTHUMBNAIL2013 - Nadia Rossi de Almeida.pdf.jpg2013 - Nadia Rossi de Almeida.pdf.jpgimage/jpeg2253http://localhost:8080/tede/bitstream/jspui/2578/18/2013+-+Nadia+Rossi+de+Almeida.pdf.jpg0d1e6343203c8558a1c8ea2b6050fbdeMD518TEXT2013 - Nadia Rossi de Almeida.pdf.txt2013 - Nadia Rossi de Almeida.pdf.txttext/plain208187http://localhost:8080/tede/bitstream/jspui/2578/17/2013+-+Nadia+Rossi+de+Almeida.pdf.txt1e97cdb6e3bb08d5189b991be61b0279MD517ORIGINAL2013 - Nadia Rossi de Almeida.pdf2013 - Nadia Rossi de Almeida.pdfapplication/pdf2617695http://localhost:8080/tede/bitstream/jspui/2578/2/2013+-+Nadia+Rossi+de+Almeida.pdfeca189b4390620fb87d9fbcf17c54ce6MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82089http://localhost:8080/tede/bitstream/jspui/2578/1/license.txt7b5ba3d2445355f386edab96125d42b7MD51jspui/25782022-09-20 16:16:41.334oai:localhost: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Biblioteca Digital de Teses e Dissertaçõeshttps://tede.ufrrj.br/PUBhttps://tede.ufrrj.br/oai/requestbibliot@ufrrj.br||bibliot@ufrrj.bropendoar:2022-09-20T19:16:41Biblioteca Digital de Teses e Dissertações da UFRRJ - Universidade Federal Rural do Rio de Janeiro (UFRRJ)false |
| dc.title.por.fl_str_mv |
Clonagem e express?o da prote?na recombinante p27 (r-p27) do v?rus da leucemia felina e produ??o de IgY avi?ria contra a r-p27 para utiliza??o em imunodiagn?stico |
| dc.title.alternative.eng.fl_str_mv |
Cloning and expression of p27 protein (r-p27) of Feline Leukemia Virus and avian IgY production against the r-p27 to immunodiagnostic application |
| title |
Clonagem e express?o da prote?na recombinante p27 (r-p27) do v?rus da leucemia felina e produ??o de IgY avi?ria contra a r-p27 para utiliza??o em imunodiagn?stico |
| spellingShingle |
Clonagem e express?o da prote?na recombinante p27 (r-p27) do v?rus da leucemia felina e produ??o de IgY avi?ria contra a r-p27 para utiliza??o em imunodiagn?stico Almeida, Nadia Rossi de FeLV imunodiagn?stico IgY immunodiagnosis Medicina Veterin?ria |
| title_short |
Clonagem e express?o da prote?na recombinante p27 (r-p27) do v?rus da leucemia felina e produ??o de IgY avi?ria contra a r-p27 para utiliza??o em imunodiagn?stico |
| title_full |
Clonagem e express?o da prote?na recombinante p27 (r-p27) do v?rus da leucemia felina e produ??o de IgY avi?ria contra a r-p27 para utiliza??o em imunodiagn?stico |
| title_fullStr |
Clonagem e express?o da prote?na recombinante p27 (r-p27) do v?rus da leucemia felina e produ??o de IgY avi?ria contra a r-p27 para utiliza??o em imunodiagn?stico |
| title_full_unstemmed |
Clonagem e express?o da prote?na recombinante p27 (r-p27) do v?rus da leucemia felina e produ??o de IgY avi?ria contra a r-p27 para utiliza??o em imunodiagn?stico |
| title_sort |
Clonagem e express?o da prote?na recombinante p27 (r-p27) do v?rus da leucemia felina e produ??o de IgY avi?ria contra a r-p27 para utiliza??o em imunodiagn?stico |
| author |
Almeida, Nadia Rossi de |
| author_facet |
Almeida, Nadia Rossi de |
| author_role |
author |
| dc.contributor.advisor1.fl_str_mv |
Mazur, Carlos |
| dc.contributor.advisor1ID.fl_str_mv |
610.764.147-53 |
| dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/7059968849728033 |
| dc.contributor.advisor-co1.fl_str_mv |
Danelli, Maria das Gra?as Miranda |
| dc.contributor.advisor-co1ID.fl_str_mv |
758.643.637-15 |
| dc.contributor.advisor-co1Lattes.fl_str_mv |
http://lattes.cnpq.br/7414712085904286 |
| dc.contributor.referee1.fl_str_mv |
Hagiwara, Mitika Kuribayashi |
| dc.contributor.referee2.fl_str_mv |
Reis, Jenner Karlisson Pimenta dos |
| dc.contributor.referee3.fl_str_mv |
McIntosh, Douglas |
| dc.contributor.referee4.fl_str_mv |
Santos, D?bora Regina Lopes dos |
| dc.contributor.authorID.fl_str_mv |
086.087.827-90 |
| dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/4292226842530490 |
| dc.contributor.author.fl_str_mv |
Almeida, Nadia Rossi de |
| contributor_str_mv |
Mazur, Carlos Danelli, Maria das Gra?as Miranda Hagiwara, Mitika Kuribayashi Reis, Jenner Karlisson Pimenta dos McIntosh, Douglas Santos, D?bora Regina Lopes dos |
| dc.subject.por.fl_str_mv |
FeLV imunodiagn?stico IgY |
| topic |
FeLV imunodiagn?stico IgY immunodiagnosis Medicina Veterin?ria |
| dc.subject.eng.fl_str_mv |
immunodiagnosis |
| dc.subject.cnpq.fl_str_mv |
Medicina Veterin?ria |
| description |
Feline Leukemia Virus (FeLV) is a gammaretrovirus, which represents an important cause of morbidity and mortality among domestic cats. In Brazil, the access to feline leukemia diagnosis is still limited due to the high cost of available commercial test to detect the viral antigen p27. The production of avian IgY instead of mammal IgG has several advantages, like lower costs of the technique, easier animal maintenance, high yields of purified antibodies and better acceptance due to bioethical characteristics. The aim of this study was the development of a recombinant viral p27 protein, the r-p27, to elicit avian antibodies, aiming to enable immunodiagnosis of FeLV infections. This technology can provide access to diagnosis and better understanding of prevalence studies. For this purpose, the coding region of the FeLV p27 protein was cloned and expressed in Escherichia coli and the reactivity against anti-p27 antibody was proven before commercial immunochromatography kit. To obtain IgG and IgY anti-p27, r-p27 was inoculated into rabbits and chickens, respectively, and the antibodies produced were tested by indirect immunofluorescence (IFA). Both immunoglobulins showed reactivity against recombinant and native p27. The production of IgY against r-p27 represents a method of high yields of purified antibodies and low cost, promising for the development of immunodiagnosis |
| publishDate |
2013 |
| dc.date.issued.fl_str_mv |
2013-02-20 |
| dc.date.accessioned.fl_str_mv |
2019-01-14T11:40:16Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
| format |
doctoralThesis |
| status_str |
publishedVersion |
| dc.identifier.citation.fl_str_mv |
ALMEIDA, Nadia Rossi de. Clonagem e express?o da prote?na recombinante p27 (r-p27) do v?rus da leucemia felina e produ??o de IgY avi?ria contra a r-p27 para utiliza??o em imunodiagn?stico. 2013. 87 f. Tese (Doutorado em Ci?ncias Veterin?rias). Instituto de Veterin?ria, Universidade Federal Rural do Rio de Janeiro, Serop?dica, 2013 |
| dc.identifier.uri.fl_str_mv |
https://tede.ufrrj.br/jspui/handle/jspui/2578 |
| identifier_str_mv |
ALMEIDA, Nadia Rossi de. Clonagem e express?o da prote?na recombinante p27 (r-p27) do v?rus da leucemia felina e produ??o de IgY avi?ria contra a r-p27 para utiliza??o em imunodiagn?stico. 2013. 87 f. Tese (Doutorado em Ci?ncias Veterin?rias). Instituto de Veterin?ria, Universidade Federal Rural do Rio de Janeiro, Serop?dica, 2013 |
| url |
https://tede.ufrrj.br/jspui/handle/jspui/2578 |
| dc.language.iso.fl_str_mv |
por |
| language |
por |
| dc.relation.references.por.fl_str_mv |
AKITA, E.M.; NAKAI, S. Immunoglobulins from egg yolk: isolation and purification. Journal of Food Science, v. 57, p. 629-634, 1992. AKITA, E.M.; NAKAI, S. Comparison of four purification methods for the production of immunoglobulins from eggs laid by hens immunized with an enterotoxigenic E. coli strain. Journal of Immunology Methods, v. 160, n. 2, p. 207-214, 1993. ALMEIDA, M.O. Clonagem e express?o do gene da toxina beta de Clostridium perfringens tipo B e sua aplica??o na imuniza??o de animais. 2010, 97p. Disserta??o (Mestrado em Gen?tica). Universidade Federal de Minas Gerais, Belo Horizonte, 2010. ALMEIDA, N.R.; DANELLI, M.G.M.; SILVA, L.H.P.; HAGIWARA, M.K., MAZUR, C. Prevalence of feline leukemia virus infection in domestic cats in Rio de Janeiro. Journal of Feline Medicine and Surgery, v. 14, n. 8, p. 583-586, 2012. ALMEIDA, N.R; ALVES, R.S.; CASTRO, A.M.T.; VALENTIM, L.F.; PISTELLI, A.L. Ocorr?ncia de linfomas em gatos com Leucemia Viral Felina atendidos no Hospital Veterin?rio de Pequenos Animais (HVPA) da UFRRJ. In: Anais da 9a Confer?ncia Sul-americana de Medicina Veterin?ria, Rio de janeiro, 2009. ALVES, FABIANA. Padroniza??o de um ELISA indireto para o diagn?stico da imunodefici?ncia felina a v?rus. 2010, 32p. Disserta??o (Mestrado em Ci?ncia Animal). Universidade Federal de Minas Gerais, Belo horizonte, 2010. ANAI, Y.; OCHI, H.; WATANABE, S., NAKAGAWA, S.; KAWAMURA, M.; GOJOBORI, T.; NISHIGAKI, K. Infectious Endogenous Retroviruses in Cats and Emergence of Recombinant Viruses. Journal of Virology, v. 86, n.16, p. 8634-8644, 2012. ARA?JO, A.S. Produ??o de antiveneno botr?pico em ovos de galinha. 2007, 57p. Disserta??o (Mestrado em Medicina Veterin?ria Preventiva). Universidade Federal de Minas Gerais, Belo Horizonte, 2007. ARA?JO, M.R.T. Express?o de prote?nas recombinantes de v?rus do g?nero Flavivirus: Aplica??o no desenvolvimento de kits de diagn?stico e em estrat?gias antivirais. 2009, 172p. Disserta??o (Mestrado em Biologia Celular e Molecular). Universidade Federal do Paran?, Curitiba, 2009. ARJONA, A.; ESCOLAR, E.; SOTO, I; BARQUERO, N.; MARTIN, D. Seroepidemiological Survey of Infection by Feline Leukemia Virus and immunodeficiency Virus in Madrid and Correlation with Some Clinical Aspects. Journal of Clinical Microbiology, v. 38, n. 9, p. 3448-3449, 2000. AUDIBERT, F. M.; LISE, L. D. Adjuvants: Current Status, Clinical Perspectives and Future Prospects. Immunology Today, v. 14, p. 281-284, 1993. AUDIBERT, F. Adjuvants for vaccines, a quest. International Immunopharmacology, v. 3, p. 1187-1193, 2003. 55 AUGUST, J.R. Mol?stias virais felinas. In: ETTINGER, S. J. Tratado de Medicina Interna Veterin?ria. 3.ed.S?o Paulo: Manole, 1992. cap.48. p. 340-346. BALAMURUGAN, V.; VENKATESAN, G.; SEN, A.; ANNAMALAI, L.; BHANUPRAKASH, V.; SINGH, R.K. Recombinant protein-based viral disease diagnostics in veterinary medicine. Expert Review of Molecular Diagnostics, v. 10, n. 6, p. 731-753, 2010. BANDE, F.; ARSHAD, S.S.; HASSAN, L.; ZAKARIA, Z.; SAPIAN, N.A.; RAHMAN, N.A.; AL-AZAWY, A. Prevalence and risk factors of feline leukaemia virus and feline immunodeficiency virus in peninsular Malaysia. BMC Veterinary Research, v. 8, n. 33, 2012. BANDECCHI, P.; DELL?OMODARME, M.; MAGI, M.; PALAMIDESSI, A.; PATRI, M.C. Feline leukaemia virus (FeLV) and feline immunodeficiency virus infections in cats in the Pisa district of Tuscany, and attempts to control FeLV infection in a colony of domestic cats by vaccination. The Veterinary Record, v. 158, p. 555-557, 2006. BANEYX, F.; MUJACIC, M. Recombinant protein folding and misfolding in Escherichia coli. Nature Biotechnology, v. 22, n. 11, p. 1399?1408, 2004. BANEYX, F. Recombinant protein expression in Escherichia coli. Current Opinion in Biotechnology, v. 10, n. 5, p. 411?421, 1999. BARBOSA, F.C.; CHRISTIANINE, M.O.T.; WALDEMARIN, K.C.A. Preval?ncia de leucemia felina em gatos dom?sticos de Uberl?ndia ? MG. Arquivo Brasileiro de Medicina Veterin?ria e Zootecnia, v. 59, n. 4, p. 939-942, 2007. BARR, F. Feline Leukemia virus. Journal of Small Animal Pratice, v. 39, n. 1, p. 41-43, 1998. BARTELING, S. J.; VREESWIJK, J. Developments in foot-and-mouth disease vaccines. Vaccine, v. 9, p. 75-88, 1991. BAYLOR, N. W.; EGAN, W.; RICHMAN, P. Aluminium salts in vaccines- US perspective. Vaccine, v. 20, n.18, 2002. BERGMAN, P.; HENDRICK, M.J.,MACY, D.; McGILL, L.; STARR, R.M.; VAN KAMPEN, K.R.Feline Sarcoma and Vaccination: Roundtable on the Injection-Site problem in cats. Veterinary Forum, v. 16, n. 3, p. 40-47, 1999. BERNARDO, A.R. Tecnologia IgY: produ??o de anticorpos avi?rios para Leishmania (Leishmania) amazonensis com o uso ?tico dos animais de experimenta??o. 2009, 49p. Disserta??o (Mestrado em Ci?ncias Veterin?rias). Universidade Federal Rural do Rio de Janeiro, Serop?dica, 2009. BIZANOV, G; JONAUSKIENA, I; HAU, F. A novel method based on lithium sulfate precipitation for purification of chicken egg yolk Immunoglobulin Y, applied to immunospecific antibodies against Sendi virus. Scandinavian Journal of Laboratory Animal Science, v. 31, p. 121-130, 2004. 56 BIZANOV, G.; NORMANTIENE, T.; JONAUSKIEN, I. Development of antibodies to Sendai virus in chickens and their isolation from yolk. Biologija, v. 2, n. 1, p. 68-71, 2006. BLANCO, K.; PRENDAS, J.; CORTES, R.; JIMENEZ, C.; DOLZ, G. Seroprevalence of Viral Infections in Domestics Cats in Costa Rice. Journal of Veterinary Medical Science. v. 71, n. 5, p. 661-663, 2009. BOLIN, L.L; LEVY, L.S. Viral Determinants of FeLV Infection and Pathogenesis: Lessons Learned from Analysis of a Natural Cohort. Viruses, v. 3, p. 1681-1698, 2010. BRADFORD, M.M. A rapid and sensitive method for the quantitation of microgram quantities utilizing the principle of protein dye binding. Analytical biochemistry, v. 72, p. 248-254, 1976. CAMPBELL, R.D.; DODDS, A.W.; PORT, R.R. The binding of human complement component C4 to antibody-antigen aggregates. The Biochemical Journal, v. 189, n. 1, p. 67-68, 1980. CARLANDER, D.; KOLLBERG, H.; WEJAKER, P.E.; LARSSON, A. Peroral immunotherapy with yolk antibodies for the prevention and treatment of enteric infections. Immunology Research, v. 21, n. 1, p. 1-6, 2000. CARROL, S.B.; THALLEY, B.S.; THEAKSTON, R.D.G. Comparison of the purity and efficacy of affinity purified avian antivenons with comercial equine crotalid antivenoms. Toxicon, v. 30, n. 9; p. 1017-1025, 1992. CHACANA, P.A.; TERZOLO, H.R.; CALAZADO, E.G.; SCHADE, R. Tecnologia IgY o aplicaciones de los anticuerpos de yema de huevo de gallina. Revista de Medicina Veterinaria, v. 85, n. 2, p. 179-189, 2004. CHALGHOUMI, R.; TH?WIS, A.; PORTETELLE, D.; BECKERS, Y. Production of hen egg yolk immunoglobulins simultaneously directed against Salmonella enteritidis and Salmonella typhimurium in the same egg yolk. Poultry Science, v. 87, n. 1, p. 32-40, 2008. CHALGHOUMI, R.; BECKERS, Y.; PORTETELLE, D.; TH?WIS, A. Hen egg yolk antibodies (IgY), production and use for passive immunization against bacterial enteric infections in chicken: a review. Biotechnology Agronomy Society and Environment, v. 13, n. 2, p.295-308, 2009. CHANG, H.M.; LU, T.C.; CHEN, C.C.; TU, Y.Y.; HWANG, J.Y. Isolation of immunoglobulin from egg yolk by anionic polysaccharides. Journal of Agricultural and Food Chemistry, v. 48, p. 995?999, 2000. CHENG, H.H.; ANDERSON, M.M.; OVERBAUGH, J. Feline leukemia virus T entry is dependent on both expression levels and specific interactions between cofactor and receptor. Virology, v. 359, p. 170-178, 2007. CHOI, J. H.; LEE, S. Y. Secretory and extracellular production of recombinant proteins using Escherichia coli. Applied Microbiology and Biotechnology, v. 64, n. 5, p. 625?635, 2004. 57 CHOU, C. P. Engineering cell physiology to enhance recombinant protein production in Escherichia coli. Applied Microbiology and Biotechnology, v. 76, n. 3, p. 521-532, 2007. COBAN, C., ISHII, K. J., GURSEL, M., KLINMAN, D. M., KUMAR, N. Effect of plasmid backbone modification by different human CpG motifs on the immunogenicity of DNA vaccine vectors. Journal of Leukocyte Biology, v. 78, n. 5, p. 647-655, 2005. COELHO, F.M.; MAIA, M.Q.; LUPPI, M.M.;COSTA, E.A.; LUIZ, A.P.M.F., RIBEIRO, N.A.; BONFIM, M.R.Q.; FONSECA, M.M.; RESENDE, M. Ocorr?ncia do v?rus da leucemia felina em Felis cattus em Belo Horizonte. Arquivo Brasileiro de Medicina Veterin?ria e Zootecnia, v. 63, n. 3, p. 778-783, 2011. COFFIN, J.M. Retroviridae: the viruses and their replication. In: FIELDS, B.N.; KNIPE, D.M.; HOWLEY, P.M.; CHANOCK, R.M.; NELMICK, J.L; MONATH, T.P.; ROZMAN, B.; STRAUS, S.E. (3ed). Fields Virology, Philadelphia: Lippincott-Raven, 1996, p. 1767-1847. COOPER, C.L.; DAVIS, H.L.; MORRIS, M.L.; EFLER, S.M.; ADHAMI, M.A.; KRIEG, A.M.; CAMERON, D.W.; HEATHCOTE, J. CpG 7909, an immunostimulatory TLR9 agonist 69 oligodeoxynucleotide, as adjuvant to Engerix-B HBV vaccine in healthy adults: a double-blind phase I/II study. Journal of Clinical Immunology. v. 24, n. 6, p. 693-701, 2004. CORNELIS, P. Expressing genes in different Escherichia coli compartments. Current Opinion in Biotechnology, v. 11, n. 5, p. 450?454, 2000. CRAWFORD, CYNDA. Progressos no diagnostico de infec??es retrovirais. In: AUGUST, J.R. Medicina Interna de Felinos. 6ed., Philadelphia, WB Saunders Company, 2011. cap. 6. p. 53-62. DAI, Y. C.; ZHANG, X.F.; TAN, M.; HUANG, P.; LEI, W.; FANG, H.; ZHONG, W.; JIANG, X. A dual chicken IgY against rotavirus and norovirus. Antiviral Research, v. 97, p. 293-300, 2012. DINGERMANN, T. Recombinant therapeutic proteins: production platforms and challenges. Biotechnology Journal, v.3, n. 1, p. 90-97, 2008. DONAHUE, P.R.; QUACKENBUSH,S.L.; GALLO, M.V.; DENORONHA, C.M.C.; OVERBAUGH, J.; HOOVER, E.A.; MULLINS, J. Viral genetic determinants of T-cell killing and immunodeficiency disease induction by the feline leukemia virus FeLV-FAIDS. Journal of Virology, v.65, p.4461-4469, 1991. DONAHUE, P.R.; HOOVER, E.A.; BELTZ, G.A.; RIEDEL, N.; HIRSCH, V.M.; OVERBAUGH, J.; MULLINS, J.I. Strong sequence conservation among horizontally transmissible, minimally pathogenic feline leukemia viruses. Journal of Virology, v. 62, n. 3, p. 722-731, 1988. DUNHAM, S.P.; GRAHAM, E. Retroviral Infections of Small Animals. Veterinary Clinics of North America: Small Animals Practice, v. 38, p. 879-901, 2008. EASTCOTT, J.W.; HOLMBERG, C.J.; DEWHIRST, F.E.; ESCH, T.R.; SMITH, D.J.; TAUBMAN, M.A. Oligonucleotide containing CpG motifs enhances immune response to 58 mucosally or systemically administered tetanus toxoid. Vaccine, v. 19, n. 13-14, p.1636-1642, 2011. EDELMAN R. Vaccine adjuvants. Reviews of Infectious Diseases, v. 2, p. 370-83, 1980. EL-MALK, M.; SHAOHONG, L.; KUMAGAI, T.; YABU, Y.; NOURELDIN, M.S.; SAUDY, N.; MARUYAMA, H.; OHTA, N. Protective Effect of Vaccination with Toxoplasma Lysate Antigen andCpG as an Adjuvant Against Toxoplasma gondii in Susceptible C57BL/6 Mice. Microbiology and Immunology, v. 49, p. 639-646, 2005. FIGUEIREDO, A.S.; ARA?JO Jr, J. P. V?rus da leucemia felina: an?lise da classifica??o da infec??o, das t?cnicas de diagn?stico e da efic?cia da vacina??o com o emprego de t?cnicas sens?veis de detec??o viral. Ci?ncia Rural, Santa Maria, v. 41, n. 11, p. 1952-1959, 2011. FLYNN, J.N., DUNHAM, S.P., WATSON, V., JARRETT, O.. Longitudinal analysis of feline leukemia virus-specific cytotoxic T lymphocytes: correlation with recovery from infection. Journal of Virology, v. 76, p. 2306- 2315, 2002. FRYER, J.; FIRCA, J.; LEVENTHAL, J.; BLONDIN, B.; MALCOLM, A.; IVANCIC, D.; GANDHI, R.; SHAH, A.; PAO, W.; ABECASSIS, M.; KAUFMAN, D.; STUART, F.; ANDERSON, B. IgY antiporcine endothelial cell antibodies effectively block human antiporcine xenoantibody binding. Xenotransplantation, v. 6, n. 2, p. 98?109, 1999. FU, C.H.; HUANG, H.; WANG, X.M.; LIU, Y.G.; WANG, Z.G.; CUI, S.J.; GAO, H.L.; LI, Z.; LI, J.P.; KONG, X.G. Preparation and evaluation of anti-SARS coronavirus IgY from yolks of immunized SPF chickens. Journal of Virology Methods. v. 133, n. 1, p. 112-115, 2006. FUJIBAYASHI, T.; NAKAMURA, M.; TOMINAGA, A.; SATOH, N.; KAWARAI, T.; NARISAWA, N. Effects of IgY against Candida albicans and Candida spp. Adherence and biofilm formation. Japanese Journal of Infections Diseases, v. 62, p. 337-342, 2009. GELLISSEN, G. Heterologous protein production in methylotrophic yeasts. Applied Microbiology and Biotechnology. v. 54, p. 741-750, 2000. GEORGIOU, G.; SEGATORI, L. Preparative expression of secreted proteins in bacteria: status report and future prospects. Current Opinion in Biotechnology, v. 16, n. 5, p.538?545, 2005. GIBERTONI, A. M.; GON?ALVES, M. C. M; MONTASSIER, M. F. S.; FERNANDES, C.C.; MONTASSIER, H. J. Clonagem, express?o e caracteriza??o da nucleoprote?na recombinante do V?rus da Bronquite Infecciosa em Escherichia coli e em Pichia pastoris. Arquivo do Instituto Biol?gico, S?o Paulo, v. 77, n.1, p.1-9, 2010. GLEICH, S.E.; KRIEGER, S.; HARTMANN K. Prevalence of feline immunodeficiency virus and feline leukaemia virus among client-owned cats and risk factors for infection in Germany. Journal of Feline Medicine and Surgery, v. 11, p. 985?992, 2009. G?MEZ, N.; SCARAMAL, J.; MIRA, G. Prevalencia de VIF y VILeF en 300 felinos en Argentina. Veterinaria Argentina, v. 16, n. 160, p. 786-793, 1999. 59 GOTTSTEIN, B.; HEMMELER, E. Egg yolk immunoglobulin Y as an alternative antibody in the serology of echinococcosis. Zeitschrift f?r Parasitenkunde, v. 71, p. 273?278, 1985. GREUNKE, K.; BRAREN, I.; ALPERS, I.; BLANK, S.; SODENKAMP, J.; BREDEHORST, R.; SPILLNER, E. Recombinant IgY for improvement of immunoglobulin-based analytical applications. Clinical Biochemistry, v. 41, n. 14-15, p. 1237-1244, 2008. GROSENBAUGH, D.A.; LEARD, T.; PARDO, M.C., MOTES-KREIMEYER, L., ROYSTON, M. Comparison of the safety and efficacy of a recombinant feline leukemia virus (FeLV) vaccine delivered transdermally and an inactivated FeLV vaccine delivered subcutaneously. Veterinary Therapeutics, v. 5, n. 4, p. 258?262, 2004. GUIMAR?ES, M.C.C.; AMARAL, L.G.; BORGES, F.V.; VIEIRA, H.P.L.; SHIMOYA, A.; MATTA, C.G.F.; MATTA, M.F.R. Production and use of egg-yolk antibody for detection of canine parvovirus in feces. Revista de Ci?ncias M?dicas e Biol?gicas, v. 7, n. 3, p. 241-248, 2008. GUPTA, R.; K, SIBER, G.R. Adjuvants for human vaccines--current status, problems and future prospects. Vaccine, v. 13, p. 1263-1276, 1995. HAGIWARA, M.K.; RECHE Jr., A; LUCAS, S.R.R. Estudo cl?nico da infec??o de felinos pelo v?rus da leucemia felina em S?o Paulo. Revista Brasileira de Ci?ncia Veterin?ria, v. 4, n .1, p. 35-38, 1997. HARDY, W.D. jr Oncogenic Virus of Cat: the leukemia and sarcoma virus. In: HOLZWORTH, J. Disease of the Cat : medicine and surgery. Philadelphia. WB Saunders Company, 1987. p. 246-268. HARDY, W.D. jr.; HIRSHAUT, Y.; HESS, P. Detection of the feline leukemia virus and other mammalian oncornaviruses by immunofluorescence. Bibliotheca Haematologica, v. 39, p. 778-799, 1973. HARDY, W.D. jr.; HESS, P.W.; MACEWEN, E.G.; MCCLELLAND, A.J.; ZUCKERMAN, E.E.; ESSEX, M.; COTTER, S. M; JARRETT, O. Biology of Feline Leukemia Virus in the Natural Environment. Cancer Research, n. 36, p. 582-588, 1976a. HARDY, W.D. jr.; McCLELLAND, A. J.; ZUCKERMAN, E. E.; HESS, P. W.; ESSEX, M.; COTTER, S. M.; C. ; HAYES, A. A. Prevention of the contagious spread of feline leukaemia virus and the development of leukaemia in pet cats. Nature, v. 263, p. 326-328, 1976b. HARTMANN, K. Feline Leukemia Virus Infection. In: GREENE, C. E. Infectious Diseases of the Dog and Cat. 3ed., Philadelphia, WB Saunders Company, 2006. cap.13. p. 105-130. HARTMANN, K.; GRIESSMAYR, P.; SCHULZ, B.; GREENE, C.E.; VIDYASHANKAR, A. N.; JARRETT, O. ; EGBERINK, H. F. Quality of different in-clinic test systems for feline immunodeficiency virus and feline leukaemia virus infection. Journal of Feline Medicine and Surgery, v. 9, p. 439-445, 2007. HATTA, H.; TSUDA, K.; AKACHI, S.; KIM, M.; YAMAMOTO, T. Productivity and some properties of egg yolk antibody (IgY) against human rotavirus compared with rabbit IgG. Bioscience, Biotechnology and Biochemistry, v. 57, n. 3, p. 450-454, 1993. 60 HELFER-HUNGERBUEHLER, K.; CATTORI, V.; BORETTI, F.S.; OSSENT, P., GREST, P.; REINACHER, M.; HENRICH, M.; BAUER, E., BAUER-PHAM, K.; NIEDERER, E.; HOLZNAGEL, E.; LUTZ, H.; HOFMANN-LEHMANN, R. Dominance of highly divergent feline leukemia virus A progeny variants in a cat with recurrent viremia and fatal lymphoma, Retrovirology, v. 7, n. 14, p. 1-14, 2010. HEMMI, H.; TAKEUCHI, O.; KAWAI, T.; KAISHO, T.; SATO, S.; SANJO, H.; MATSUMOTO, M.; HOSHINO, K.; WAGNER, H.; TAKEDA, K.; AKIRA, S. A Toll-like receptor recognizes bacterial DNA. Nature, v. 408, p.740?745, 2000. HERRING, E.S; TROY, G.C.; TOTH, T.E; FORRESTER, S.D.; WEIGT, L.A.; HERRING, I.P. Detection of feline leukaemia virus in blood and bone marrow of cats with varying suspicion of latent infection. Journal of Feline Medicine and Surgery, v. 3, n. 3, p. 133-141, 2001. HILLEMAN, M. R. Critical appraisal of emulsified oil adjuvants applied to viral vaccine. Progress in Medical Virology, v. 8, p. 131-182, 1966. HIRAI, K.; ARIMITSU, H.; UMEDA, K.; YOKOTA, K.; SHEN, L.; AYADA, K. Passive oral immunization by egg yolk immunoglobulin (IgY) to Vibrio cholerae effectively prevents cholera. Acta Medica Okayama. v. 64, p. 163-170, 2010. HOFMANN-LEHMANN, R.; VALENTINO CATTORI, V.; RAVI TANDON, R.; BORETTI, F.S.; MELI, M.L.; RIOND, B.; LUTZ, H. How molecular methods change our views of FeLV infection and vaccination. Veterinary Immunology and Immunopathology, v. 123, p. 119?123, 2008. HOOVER, E.A.; MULLINS, J. I. Feline leukemia virus infection and diseases. Journal of American Veterinary Medical Association, v. 199, p.1287-1297, 1991. HORIKOSHI, T.; HIRAOKA, J.; SAITO, M.; HAMADA, S. IgY antibody from hen egg yolks: purification by ethanol fractionation. Journal of Food Science, v. 58, n. 4, p. 739-742, 1993. HUOPALAHTI, R. et al. 2007. In: Bioactive Egg Compounds. Dieter Czeschlik (ed.), Springer-Verlag Berlin Heidelberg. 297 p. ISMAIL, A. A. A radical approach is needed to eliminate interference from endogenous antibodies in immunoassays. Clinical Chemistry, v. 51, n. 1, p. 25-26, 2005. JANA, S.; DEB, J. K.; Strategies for efficient production of heterologous proteins in Escherichia coli. Applied Microbiology and Biotechnology, v. 67, n. 3, p. 289-98, 2005. JARRETT, O.; LAIRD, H.M.; HAY, D. Determinants of the host range of feline leukemia viruses. Journal of General Virology, v. 20, n. 2, p. 169-175, 1973. JARRETT, O. Strategies of retrovirus survival in the cat. Veterinary Microbiology, v. 69, p. 99-107, 1999. JIN, W.; YAMADA, K.; IKAMI, M.; KAJI, N.; TOKESHI, M.; ATSUMI, Y.; MIZUTANI, M.; MURAI, A.; OKAMOTO, A.; NAMIKAWA, T.; BABA, Y.; OHTA, M. Application of 61 IgY to sandwich enzyme-linked immunosorbent assays, lateral flow devices, and immunopillar chips for detecting staphylococcal enterotoxins in milk and dairy products. Journal of Microbiological Methods, 2013. No prelo. JONASSON, P.; LILJEQVIST, S.; NYGREN, P. A.; STAHL, S. Genetic design for facilitated production and recovery of recombinant proteins in Escherichia coli. Biotechnology Applied Biochemistry, v. 35, n. 2, p.91?105, 2002. JUNQUEIRA-JORGE, J.; FERREIRA, F.; HAGIWARA, M.K. Risk factors for feline leukemia virus (FeLV) infection in cats in S?o Paulo, Brazil. Brazilian Journal Veterinary Research Animal Science, v. 48, n. 5, p. 392-398, 2011. KADIOGLU, A.; COWARD, W.; COLSTON, M.J.; HEWITT, C.R.A.; ANDREW, P.W.; CD4-T-Lymphocyte Interactions with Pneumolysin and Pneumococci Suggest a Crucial Protective Role in the Host Response to Pneumococcal Infection. Infection and Immunity, v. 72, n. 5, p. 2689-97, 2004. KANO, F.S.; VIDOTTO, O.; VIDOTTO, M.C. Vacina de DNA: aspectos gerais e sua aplica??o na medicina humana e veterin?ria. Ci?ncias Agr?rias, v. 28, n. 4, p. 709-726, 2007. KAPOOR, P.; COMPTON, M.M.; HOWARTH, B. Immunization of chickens with quail and turkey perivitelline membrane proteins: production of antibodies and their effects on fertility. Poultry Science, v. 79, n. 2, p. 245-256, 2000. KLEMPERER F. Ueber nat?rliche lmmunit?t und ihre Verwerthung f?r die Immunisirungstherapie. Archiv f?r Experimentelle Pathologie und Pharmakologie v.31, p. 356-382, 1883 apud Narat M. Production of antibodies in chickens. Food Technology Biotechnology v. 41, n.3, p. 259-267, 2003. KLINMAN, D.M.; AE-KYUNG, Y.; BEAUCAGE, S.L.; CONOVER, J.; KRIEG, A.M. CpG Motifs Present in Bacterial DNA Rapidly Induce lymphocytes to Secrete Interleukin 6, Interleukin 12, and Interferon Gamma. Proceedings of the National Academy of Sciences of the United States of America, v. 93, p. 2879-2883, 1996. KLINMAN, D.M.; BARNHART, K.M.; CONOVER, J. CpG motifs as immune adjuvants. Vaccine. v.17, p.19-25, 1999. KNOTEK, Z.; HADJOKOVA, P.; SVOBODA, M.; TOMAN, M.; RASKA, V. Epidemiology of feline leukaemia and feline immunodeficiency virus in Czech Republic. Journal of Veterinary Medicine Series, v. 26, n. 10, p. 665-671, 1999. KOLLBERG, H.; CARLANDER D.; OLESEN H.; WEJAKER, P.E.; JOHANNESSON, M.; LARSSON, A. Oral administration of specific yoIk antibodies (IgY) may prevent Pseudomonas aeruginosa infections in patients with cystic fibrosis: a phase I feasibility study. Pediatric Pulmonology, v. 35, p. 433-440, 2003. KRIEG, A.M.; AE-KYUNG, Y.; MATSON, S.; WALDSCHMIDT, T.J.; BISHOP, G.A.; TEASDALE, R.; KORETZKY, G.A.; KLINMAN, D.M. CpG Motifs in Bacterial DNA Trigger Direct B-cell Activation. Nature, v. 374, p. 546-549, 1995. 62 KURLAND, C.; GALLANT, J.; Errors of heterologous protein expression. Current Opinion in Biotechnology, v. 7, n. 5, p. 489-93, 1996. LAPPIN, M.R. Feline Leukemia Virus. In: Semin?rio Internacional de Doen?as Infecciosas, 1998 , S?o Paulo. Anais...p. 36-44. LARENTIS, A. L.; ARGONDIZZO, A.P.C.; ESTEVES, G.S.; JESSOURON, E.; GALLER, R.; MEDEIROS, M.A. Cloning and optimization of induction conditions for mature PsaA (pneumococcal surface adhesin A) expression in Escherichia coli and recombinant protein stability during long-term storage. Protein Expression and Purification, v. 78, n. 1, p. 38-47, 2011. LARSSON, A.; KARLSSON-PARRA, A.; SJOQUIST, J. Use of chicken antibodies in enzyme immunoassays to avoid interference by rheumatoid factors. Clinical Chemistry, v. 37, p. 411-414, 1991. LEE, E.N.; SUNWOO, H.H.; MENNINEN, K.; SIM, J.S. In vitro studies of chicken egg yolk antibody (IgY) against Salmonella enteritidis and Salmonella typhimurium. Poultry Science, v. 81, p. 632-641, 2002. LEE, S. Y.; CHOI, J. H.; XU, Z. Microbial cell-surface display. Trends in Biotechnology, v. 21, n. 1, p. 45?52, 2003. LEV?SQUE, S.; MARTINEZ, G.; FAIRBROTHER, J.M. Improvement of adjuvant systems to obtain a cost-effective production of high levels of specific IgY. Poultry Science, v.86, p. 630?635, 2007. LEVY, J.; CRAWFORD, P. C.; HARTMANN, K.; HOFMANN-LEHMANN, R.; LITTLE, S.; SUNDAHL, E. American Association of Feline Practitioners? feline retrovirus management guidelines. Journal of Feline and Surgery, v. 10, n .3, p.300-316, 2008. LEVY, J. K.; SCOTT, H. M.; LACHTARA, J. L.; CRAWFORD, P. C. Seroprevalence of feline leukemia virus and feline immunodeficiency virus infection among cats in North America and risk factors for seropositivity. Journal of the American Veterinary Medical Association, v. 228, n .3, p. 371-376, 2006. LIN, J.A.; CHENG, M.C.; INOSHIMA, Y; TOMONAGA, K.; MIYAZAWA, T.; TOHYA, Y.; TOH, K.; LU, Y.S.; MIKAMI, T. Seroepidemiological survey of feline retrovirus infections in cats in Taiwan in 1993 and 1994. Journal of Veterinary Medical Science, v. 57, n. 1, p. 161-163, 1995. LINDAHL, T.L.; FESTIN, R.; LARSSON, A. Studies of fibrinogen binding to platelets by flow cytometry: An improved method for detection of platelet activation. Thrombosis and Haemostasis, v. 68, p. 221-225, 1992. LINDBLAD, E. Aluminium adjuvants. In: Stewart- Tull, D. Editor. The theory and practical application of adjuvants. New York: Wiley, p. 21- 35, 1995. LINENBERGER, M.L.; ABKOWITZ, J.L. Haematological disorders associated with feline retrovirus infections. Bailli?re's clinical haematology, v. 8, n. 1, p. 73-112, 1995. 63 LIOU, J.F; CHANG, C.W.; TAILIU, J.J.; YU, C.K.; LEI, H.Y.; CHEN, L.R. Passive protection effect of chicken egg yolk immunoglobulins on enterovirus 71 infected mice. Vaccine, v. 28, p. 8189-8196, 2010. LITTLE, S.; SEARS, W.; LACHTARA, J.; BIENZLE, D. Seroprevalence of feline leukemia virus and feline immunodeficiency virus infection among cats in Canada. The Canadian Veterinary Journal, v. 50, p. 644?648, 2009. LOAR, A.S. The controversy continues. In: THE FIFTH ANNUAL VETERINARY MEDICAL FORUM, 15, 1987. Proceedings?American College of Veterinary Internal Medicine. 1987. p. 338-342. LOAR, A.S. Feline Leukemia virus: Imunization and Prevention. Veterinary Clinics of North America: Small Animal Practice, v.23, n.1, p. 193-211, 1993. LOSSO, J. N.; KUMMER, A.; LI-CHAN, E.; NAKAI, S. Development of a particle concentration fluorescence immunoassay for the quantitative determination of IgG in bovine milk. Journal of agricultural and food chemistry, v. 41, p. 682-686, 1993. LUCAS, S.R.R; HAGIWARA, M.K.; RECHE JR., A.; GERMANO, P.M.L. Ocorr?ncia de anticorpos antitoxoplasma em gatos infectados naturalmente pelo v?rus da imunodefici?ncia dos felinos. Brazilian Journal of Veterinary Research and Animal Science, v. 35, n. 1, p. 41-45, 1998. LUCHESE, M.D.; ARGONDIZZO, A.P.C.; JESSOUROUN, E.; PEREIRA, D.S.G.; ANDRADEGOES, A.C.M.; ARDENGHI, P.G.; MEDEIROS, M.A.; LARENTIS, A.L. Influ?ncia de Diferentes Condi??es na Express?o da Prote?na Recombinante Pneumolisina (rPly). Anais do XVII Simp?sio Nacional de Bioprocessos. Sinaferm, 2009. LUTZ, H; ADDIE, D; BELAK, S. et al. Feline leukaemia ABCD guidelines on prevention and management. Journal of Feline Medicine and Surgery, v.11, p. 565-74, 2009. MACIEIRA, D.B.; MENEZES, R.C..A.A.; DAMICO, C.B; ALMOSNY, N.R.P., MCLANE, H.L.; DAGGY, J.K.; MESSICK, J.B. Prevalence and risk factors for hemoplasmas in domestic cats naturally infected with feline immunodeficiency virus and/or feline leukemia virus in Rio de Janeiro - Brazil. Journal of Feline Medicine and Surgery, v. 10, p. 120-129, 2008. MACY, D.W.; HENDRICK, M.J. The potential role of inflammation in the development of postvaccinal sarcomas in cats. Veterinary Clinics of North America: Small Animal Practice, v. 26 , n. 1 , p. 103-109, 1996. MCLAREN, R.D.; PROSSER, C.G.; GRIEVE, R.C.J. The use of caprylic acid for extraction of immunoflobulin fraction from egg yolk of chickens immunized with ovine ?-lactalbumin. Journal of Immunological Methods, v. 177, n. 1, p. 175-184, 1994. MADEWELL, B.R.; JARRET, O. Recovery of feline leukaemia virus from nonviraemic cats. Veterinary Record, v. 112, n. 1, p. 90-93, 1983. MAHMOOD, M.S.; SIDDIQUE, M.; HUSSAIN, I.; KHAN, A.; MANSOOR, M.K. Protection capability of recombinant plasmid DNA vaccine containing VP2 gene of very 64 virulent infectious bursal disease virus in chickens adjuvanted with CpG oligodeoxynucleotide. Vaccine, v. 24, n. 22, p. 4838-4846, 2006. MAKRIDES, S. C. Strategies for achieving high-level expression of genes in Escherichia coli. Microbiological Reviews, v. 60, n. 3, p. 521-538, 1996. MARCIANI, D.J.; KENSIL, C.R.; BELTZ, G.A.; HUNG, C.H.; CRONIER, J.; AUBERT, A. Genetically-engineered subunit vaccine against feline leukaemia virus: protective immune response in cats. Vaccine, v. 9, 1991. MAZUR, C.; REIS, J.K.P.; LEITE, R.C.; DANELLI, M.G.M.; HAGIWARA, M.K.; G?ES, A.C.M.A.; MEDEIROS, M.A. Evaluation of a recombinant p24 antigen for the detection of Feline Immunodeficiency Virus-specific antibodies. Pesquisa Veterin?ria Brasileira, v. 30; n. 10, p. 877-880, 2010. MEHL, M.L. Feline Leukemia virus. In: LAPPIN, M.R. Feline International Medicine Secrets, Philadelphia: Hanley & Belfus. 2001. cap.76. p. 387-391. MEINERZ, A.R.M.; ANTUNES, T.A.; SOUZA, L.L.; NASCENTE, P.S.; FARIA, R.O.; CLEFF, M.B.; GOMES, F.R.; NOBRE, M. S.O; REISCHAK, D.; SCHUCH, L.F.D.; MEIRELES, M.C.A. Frequ?ncia do v?rus da leucemia felina (vlfe) em felinos dom?sticos (felis catus) semidomiciliados nos munic?pios de Pelotas e Rio Grande. Ci?ncia Animal Brasileira, v. 11, n. 1, p. 90-93, 2010. MENDOZA, R.; ANDERSON, M.M.; OVERBAUGH, J. A Putative Thiamine Transport Protein Is a Receptor for Feline Leukemia Virus Subgroup A. Journal of Virology, v. 80, p. 3378?3385, 2006. MEULENAER, B.; HUYGHEBAERT, A. Isolation and purification of chicken egg yolk immunoglobulins: a review. Food and Agricultural Immunology, v. 13, n. 1, p. 275- 288, 2001. MINE, Y.; KOVACS-NOLAN, J. Chicken egg yolk antibodies as therapeutics in enteric infectious disease: a review. Journal of Medical Food, v. 5, n. 3, p. 159-169, 2002. MIYAZAWA, T. Infections of feline leukemia virus and feline immunodeficiency virus. Front Biosci, v. 7, p. 504-518, 2002. MOORE, A.S; OGILVIE, G.K. Lymphoma in Feline Oncology: A Comprehensive Guide to Compassionate Care. Veterinary Learning Systems, p. 191-219, 2001. MOORE, G. E.; WARD, M. P.; DHARIWAL, J. Use of a primary care veterinary medical database for surveillance of syndromes and diseases in dogs and cats. Journal of Veterinary Internal Medicine, v. 18, p. 386, 2004. MOREIRA, A.S.A.S. Produ??o antiveneno botr?pico em ovos de galinha. 2007. 48 p. Disserta??o (Mestrado em Ci?ncia Animal) - Universidade Federal de Minas Gerais, Belo Horizonte, 2007. 65 MORRISON, S.L.; MOHAMMED, M.S.; WIMS, L.A.; TRINH, R.; ETCHES, R. Sequences in antibody molecules important for receptor-mediated transport into the chicken egg yolk. Molecular Immunology, v. 38, n. 8, p.619-625, 2001. MOTOI, Y.; INOUE, S.; HATTA, H.; SATO, K.; MORIMOTO, K.; YAMADA, A.; Detection of rabies-specific antigens by egg yolk antibody (IgY) to the recombinant rabies v?rus proteins produced in Escherichia coli. Japanese Journal of Infectious Diseases, v. 58, p. 115-118, 2005. NARAT M. Production of antibodies in chickens. Food Technology and Biotechnology, v. 41, n.3, p. 259-267, 2003 |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
application/pdf |
| dc.publisher.none.fl_str_mv |
Universidade Federal Rural do Rio de Janeiro |
| dc.publisher.program.fl_str_mv |
Programa de P?s-Gradua??o em Ci?ncias Veterin?rias |
| dc.publisher.initials.fl_str_mv |
UFRRJ |
| dc.publisher.country.fl_str_mv |
Brasil |
| dc.publisher.department.fl_str_mv |
Instituto de Veterin?ria |
| publisher.none.fl_str_mv |
Universidade Federal Rural do Rio de Janeiro |
| dc.source.none.fl_str_mv |
reponame:Biblioteca Digital de Teses e Dissertações da UFRRJ instname:Universidade Federal Rural do Rio de Janeiro (UFRRJ) instacron:UFRRJ |
| instname_str |
Universidade Federal Rural do Rio de Janeiro (UFRRJ) |
| instacron_str |
UFRRJ |
| institution |
UFRRJ |
| reponame_str |
Biblioteca Digital de Teses e Dissertações da UFRRJ |
| collection |
Biblioteca Digital de Teses e Dissertações da UFRRJ |
| bitstream.url.fl_str_mv |
http://localhost:8080/tede/bitstream/jspui/2578/18/2013+-+Nadia+Rossi+de+Almeida.pdf.jpg http://localhost:8080/tede/bitstream/jspui/2578/17/2013+-+Nadia+Rossi+de+Almeida.pdf.txt http://localhost:8080/tede/bitstream/jspui/2578/2/2013+-+Nadia+Rossi+de+Almeida.pdf http://localhost:8080/tede/bitstream/jspui/2578/1/license.txt |
| bitstream.checksum.fl_str_mv |
0d1e6343203c8558a1c8ea2b6050fbde 1e97cdb6e3bb08d5189b991be61b0279 eca189b4390620fb87d9fbcf17c54ce6 7b5ba3d2445355f386edab96125d42b7 |
| bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 MD5 |
| repository.name.fl_str_mv |
Biblioteca Digital de Teses e Dissertações da UFRRJ - Universidade Federal Rural do Rio de Janeiro (UFRRJ) |
| repository.mail.fl_str_mv |
bibliot@ufrrj.br||bibliot@ufrrj.br |
| _version_ |
1791083879104249856 |