Avalia??o morfol?gica, eletromiogr?fica e por termografia infravermelha do bloqueio do plexo braquial em coelhos guiado por ultrassonografia

Detalhes bibliográficos
Autor(a) principal: Moreira, Rodrigo Mencalha
Data de Publicação: 2016
Tipo de documento: Tese
Idioma: por
Título da fonte: Biblioteca Digital de Teses e Dissertações da UFRRJ
Texto Completo: https://tede.ufrrj.br/jspui/handle/jspui/1473
Resumo: The brachial plexus block (BPB) remains one of the most intriguing topics of contemporary anesthesia because, due to the complex organization of this structure is associated with a significant number of failures. The aim of this study was to evaluate the effectiveness of ultrasound-guided associated with peripheral nerve stimulation BPB in rabbits. Initially, 80 plexus were dissected, so as to enable researchers in the gross anatomy of the region. Later, in the in vivo study, we used 40 male rabbits which were randomly divided into two groups: Group 1: Ultrasound-guided associated with peripheral nerve stimulation (US/ENP) BPB; Group 2: Peripheral nerve stimulation-guided (ENP) BPB. Under general anesthesia, axillary BPB was performed, under lidocaine 2% without vasoconstrictor injection at the maximum dose of 0,7ml.kg-1. The motor latency, motor block and volume difference between the BPB between the techniques were evaluated by recording motor action potentials compounds of the radial nerve. The measurement of skin temperature (ST), by infrared thermography, was carried out in areas of interest, previously stipulated in the forepaw, digits and forearm, in order to verify the correlation of the variation in the effectiveness of BPB. In 92.5% of the animals the nerves resulting consisted of connections between the ventral rami of the last four cervical spinal nerves (C5, C6, C7, C8) and first thoracic (T1). No significant difference was observed in time performance of US/ENP-guided BPB (4.3 ? 0.73 min) or ENP-guided BPB (6.4 ? 0.68 minutes), however, significantly less volume administration was necessary to local anesthetic in the US/ENP-guided BPB (0.61 ? 0.15 mL vs 1.22 ? 0.17; P <0.0001). Despite the lower volume used, it was observed that the US/ENP group had shorter on set time block (1.1 ? 0.45) compared to ENP group (1.95 ? 0.79; P <0.01). The US/ENP-guided BPB or ENP-guided BPB resulted in a substantial and significant increase in ST areas of interest in the radial, musculocutaneous, median and ulnar nerves (P < 0.001), however this increase was outstanding in hand and digits regions Thus it is concluded that the US/ENP-guided BPB is an effective and easy technique to reproduce in this experimental model which required lower volume of local anesthetic, provided a smaller motor latency and a higher motor blockade time when compared to the ENP-guided BPB. The increase in ST is a highly effective tool for assessing the effectiveness of BPB with predictive value, sensitivity and specificity of 100%. Furthermore, future clinical studies are needed to verify its correlation with the anaesthetized area.
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spelling Figueiredo, Marcelo Abidu986.393.587-53http://lattes.cnpq.br/0514197225370792Otero, Pablo Ezequielhttp://lattes.cnpq.br/2699593202605569Sampaio, Marco Aur?lio PereiraSouza, Diogo Benchimol deSantos, Paulo Sergio Patto dosFigueiredo, Nubia Ver?osa8438065759http://lattes.cnpq.br/5427334834841231Moreira, Rodrigo Mencalha2017-03-22T12:11:48Z2016-04-28MOREIRA, Rodrigo Mencalha. Avalia??o morfol?gica, eletromiogr?fica e por termografia infravermelha do bloqueio do plexo braquial em coelhos guiado por ultrassonografia. 2016. 79 f. Tese (Doutorado em Medicina Veterin?ria, Patologia e Ci?ncias Cl?nicas) - Instituto de Veterin?ria, Universidade Federal Rural do Rio de Janeiro, Serop?dica - RJ, 2016.https://tede.ufrrj.br/jspui/handle/jspui/1473The brachial plexus block (BPB) remains one of the most intriguing topics of contemporary anesthesia because, due to the complex organization of this structure is associated with a significant number of failures. The aim of this study was to evaluate the effectiveness of ultrasound-guided associated with peripheral nerve stimulation BPB in rabbits. Initially, 80 plexus were dissected, so as to enable researchers in the gross anatomy of the region. Later, in the in vivo study, we used 40 male rabbits which were randomly divided into two groups: Group 1: Ultrasound-guided associated with peripheral nerve stimulation (US/ENP) BPB; Group 2: Peripheral nerve stimulation-guided (ENP) BPB. Under general anesthesia, axillary BPB was performed, under lidocaine 2% without vasoconstrictor injection at the maximum dose of 0,7ml.kg-1. The motor latency, motor block and volume difference between the BPB between the techniques were evaluated by recording motor action potentials compounds of the radial nerve. The measurement of skin temperature (ST), by infrared thermography, was carried out in areas of interest, previously stipulated in the forepaw, digits and forearm, in order to verify the correlation of the variation in the effectiveness of BPB. In 92.5% of the animals the nerves resulting consisted of connections between the ventral rami of the last four cervical spinal nerves (C5, C6, C7, C8) and first thoracic (T1). No significant difference was observed in time performance of US/ENP-guided BPB (4.3 ? 0.73 min) or ENP-guided BPB (6.4 ? 0.68 minutes), however, significantly less volume administration was necessary to local anesthetic in the US/ENP-guided BPB (0.61 ? 0.15 mL vs 1.22 ? 0.17; P <0.0001). Despite the lower volume used, it was observed that the US/ENP group had shorter on set time block (1.1 ? 0.45) compared to ENP group (1.95 ? 0.79; P <0.01). The US/ENP-guided BPB or ENP-guided BPB resulted in a substantial and significant increase in ST areas of interest in the radial, musculocutaneous, median and ulnar nerves (P < 0.001), however this increase was outstanding in hand and digits regions Thus it is concluded that the US/ENP-guided BPB is an effective and easy technique to reproduce in this experimental model which required lower volume of local anesthetic, provided a smaller motor latency and a higher motor blockade time when compared to the ENP-guided BPB. The increase in ST is a highly effective tool for assessing the effectiveness of BPB with predictive value, sensitivity and specificity of 100%. Furthermore, future clinical studies are needed to verify its correlation with the anaesthetized area.O bloqueio do plexo braquial (BPB) permanece como um dos temas mais intrigantes da anestesia contempor?nea, pois, devido a complexa organiza??o desta estrutura, est? associado a um n?mero expressivo de insucessos. Com o estudo objetivou-se avaliar a efic?cia do bloqueio do BPB guiado por ultrassonografia associado a estimula??o de nervos perif?rico em coelhos. Inicialmente foram dissecados 80 plexos braquiais, de 40 cad?veres, de modo a capacitar os pesquisadores na anatomia macrosc?pica da regi?o. Posteriormente, no estudo in vivo, foram utilizados 40 coelhos do sexo masculino os quais foram aleatoriamente alocados em dois grupos experimentais: Grupo 1: BPB guiado por ultrassonografia associado a estimula??o de nervos perif?ricos (US/ENP); Grupo 2: BPB guiado por estimula??o de nervos perif?rivos (ENP). Sob anestesia geral, o BPB foi realizado, por via axilar, atrav?s da inje??o de lidoca?na 2% sem vasoconstrictor, na dose m?xima de 0,7ml.kg-1. A diferen?a entre o tempo de lat?ncia motora, tempo de bloqueio motor e do volume necess?rio para o BPB entre as t?cnicas foram avaliadas atrav?s da grava??o dos potenciais de a??o motores compostos do nervo radial. A mensura??o da temperatura cut?nea (TC), por termografia infravermelha, foi realizada em ?reas de interesse (AIEs), previamente estipuladas, nas m?os, d?gitos e antebra?os, de modo a verificar a correla??o de sua varia??o com a efic?cia do BPB. Em 92,5% dos animais os nervos resultantes foram constitu?dos das conex?es entre os ramos ventrais dos 4 ?ltimos nervos espinhais cervicais (C5, C6, C7, C8) e o primeiro tor?cico (T1). N?o houve diferen?a significativa no tempo de performance do BPB guiado por US/ENP (4,3 ? 0,73 min) ou por ENP (6,4 ? 0,68 min), no entanto, foi necess?rio a administra??o de um volume significativamente menor de anest?sico local no grupo guiado por US/ENP (0,61 ? 0,15 ml versus 1,22 ? 0,17; P < 0,0001). Apesar do menor volume utilizado, observou-se que o grupo US/ENP apresentou menor tempo para instala??o do bloqueio (1,1 ? 0,45) em compara??o ao grupo ENP (1,95 ? 0,79; P < 0,01). O BPB guiado por US/ENP ou por ENP resultou em um aumento substancial e significativo da TC nas AIEs dos nervos radial, musculocut?neo ulnar e mediano (P < 0,001), no entanto, este aumento foi mais contudente na regi?o da m?o e d?gitos. Nas AIEs dos nervos radial, mediano e ulnar na regi?o dorsolateral das m?os, foram observadas as maiores varia??es de temperatura no grupo US/ENP em compara??o ao ENP (radial 3,1 versus 2,0oC; mediano 4,5 versus 3,1 oC e ulnar 4,1 versus 3,6 oC). Dessa forma conclui-se que o BPB guiado por US/ENP ? uma t?cnica eficaz e de f?cil reprodutibilidade no modelo experimental utilizado a qual requereu menor volume de anest?sico local, proporcionou menor tempo de lat?ncia motora e maior tempo de bloqueio motor quando comparado ao bloqueio guiado por ENP. O aumento da TC ? uma ferramenta altamente eficaz na avalia??o da efic?cia do BPB com valor preditivo, sensibilidade e especificidade de 100%. Outrossim,futuros estudos cl?nicos s?o necess?rios para verificar sua correla??o com a ?rea anestesiada.Submitted by Sandra Pereira (srpereira@ufrrj.br) on 2017-03-22T12:11:48Z No. of bitstreams: 1 2016 - Rodrigo Mencalha Moreira.pdf: 28440057 bytes, checksum: 791e667a1aa1132fd32db5ed549faf27 (MD5)Made available in DSpace on 2017-03-22T12:11:48Z (GMT). No. of bitstreams: 1 2016 - Rodrigo Mencalha Moreira.pdf: 28440057 bytes, checksum: 791e667a1aa1132fd32db5ed549faf27 (MD5) Previous issue date: 2016-04-28Funda??o Carlos Chagas Filho de Amparo ? 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Bosnian journal of basic medical sciences, v. 5, n. 3, p. 79-85, 2005. WAAG, S.; STOFFEL, M.H.; SPADAVECCHIA, C.; EICHENBERGER, U.; ROHRBACH, H. Ultrasound-guided block of sciatic and femoral nerves: an anatomical study. Laboratory Animals, v. v. 48, n. 2, p. 97-104, 2014. WAKOFF, T.I.; MENCALHA, R.; SOUZA, N.S.; SOUZA, M.D.; SANTOS-SOUSA, C.A.; SCHERER, P.O. Bupivaca?na 0,25% versus ropivaca?na 0,25% no bloqueio do plexo braquial em c?es da ra?a beagle. Semina Ci?ncias Agr?rias, v. 34, p. 1259-1272, 2013. WEIL, M.; LITZKE, L.F.; FRITSCH, R. Diagnostic validity of the thermography of lameness in horses. Diagostic validity of thermography of lameness in horses. Tier?rztliche Praxis. Ausgabe G, Grosstiere/Nutztiere, v. 26, n. 6, p. 346-354, 1998. 78 WERDEHAUSEN, R.; BUCHNER, S.; HERMANNS, H.; FREYNHAGEN, R.; LIPFERT, P.; STEVENS, M.F. Uniform distribution of skin-temperature increses after different regional-anesthesia techniques of the extremity. Regional Anesthesia and Pain Medicine, v. 32, n.1, p. 73-78, 2007. WESTERMANN, S.; BUCHNER, H.H.; SCHRAMEL, J.P.; TICHY, A.; STANEK, C. Effects of infrared camera angle and distance on measurement and reproducibility of thermographically determined temperatures of the distolateral aspects of the forelimbs in horses. Journal of American Veterinary and Medical Association, v. 242, n. 3, p. 388-395, 2013. WHITTAKER, S.; LETHBRIDGE, G.; KIM, C.; KEON COHEN, Z.; NG, I. An ultrasound needle insertion guide in a porcine phantom model. Anaesthesia, v. 68, n.8, p. 826-829, 2013. WILLIAMS, S.R.; CHOUINARD, P.; ARCAND, G.; HARRIS, P.; RUEL, M.; BOUDREAULT, D.; GIRARD, F. Ultrasound guidance speeds execution and improves the quality of supraclavicular block. Anesthesia and Analgesia, v. 97, n. 5, p. 1518-1523, 2003. WRIGHT, H.M.; KORR, I.M.; THOMAS, P.E. Local and regional variations in cutaneous vasomotor tone of the human trunk. 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dc.title.por.fl_str_mv Avalia??o morfol?gica, eletromiogr?fica e por termografia infravermelha do bloqueio do plexo braquial em coelhos guiado por ultrassonografia
dc.title.alternative.eng.fl_str_mv Morphological, electromyography and infrared thermal imaging evaluation of the ultrassound-guided brachial plexus block in rabbits
title Avalia??o morfol?gica, eletromiogr?fica e por termografia infravermelha do bloqueio do plexo braquial em coelhos guiado por ultrassonografia
spellingShingle Avalia??o morfol?gica, eletromiogr?fica e por termografia infravermelha do bloqueio do plexo braquial em coelhos guiado por ultrassonografia
Moreira, Rodrigo Mencalha
Brachial plexus
Ultrasonography
Plexo braquial
Ultrassonografia
Termografia
Termography
Medicina Veterin?ria
title_short Avalia??o morfol?gica, eletromiogr?fica e por termografia infravermelha do bloqueio do plexo braquial em coelhos guiado por ultrassonografia
title_full Avalia??o morfol?gica, eletromiogr?fica e por termografia infravermelha do bloqueio do plexo braquial em coelhos guiado por ultrassonografia
title_fullStr Avalia??o morfol?gica, eletromiogr?fica e por termografia infravermelha do bloqueio do plexo braquial em coelhos guiado por ultrassonografia
title_full_unstemmed Avalia??o morfol?gica, eletromiogr?fica e por termografia infravermelha do bloqueio do plexo braquial em coelhos guiado por ultrassonografia
title_sort Avalia??o morfol?gica, eletromiogr?fica e por termografia infravermelha do bloqueio do plexo braquial em coelhos guiado por ultrassonografia
author Moreira, Rodrigo Mencalha
author_facet Moreira, Rodrigo Mencalha
author_role author
dc.contributor.advisor1.fl_str_mv Figueiredo, Marcelo Abidu
dc.contributor.advisor1ID.fl_str_mv 986.393.587-53
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/0514197225370792
dc.contributor.advisor-co1.fl_str_mv Otero, Pablo Ezequiel
dc.contributor.advisor-co1Lattes.fl_str_mv http://lattes.cnpq.br/2699593202605569
dc.contributor.referee1.fl_str_mv Sampaio, Marco Aur?lio Pereira
dc.contributor.referee2.fl_str_mv Souza, Diogo Benchimol de
dc.contributor.referee3.fl_str_mv Santos, Paulo Sergio Patto dos
dc.contributor.referee4.fl_str_mv Figueiredo, Nubia Ver?osa
dc.contributor.authorID.fl_str_mv 8438065759
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/5427334834841231
dc.contributor.author.fl_str_mv Moreira, Rodrigo Mencalha
contributor_str_mv Figueiredo, Marcelo Abidu
Otero, Pablo Ezequiel
Sampaio, Marco Aur?lio Pereira
Souza, Diogo Benchimol de
Santos, Paulo Sergio Patto dos
Figueiredo, Nubia Ver?osa
dc.subject.eng.fl_str_mv Brachial plexus
Ultrasonography
topic Brachial plexus
Ultrasonography
Plexo braquial
Ultrassonografia
Termografia
Termography
Medicina Veterin?ria
dc.subject.por.fl_str_mv Plexo braquial
Ultrassonografia
Termografia
Termography
dc.subject.cnpq.fl_str_mv Medicina Veterin?ria
description The brachial plexus block (BPB) remains one of the most intriguing topics of contemporary anesthesia because, due to the complex organization of this structure is associated with a significant number of failures. The aim of this study was to evaluate the effectiveness of ultrasound-guided associated with peripheral nerve stimulation BPB in rabbits. Initially, 80 plexus were dissected, so as to enable researchers in the gross anatomy of the region. Later, in the in vivo study, we used 40 male rabbits which were randomly divided into two groups: Group 1: Ultrasound-guided associated with peripheral nerve stimulation (US/ENP) BPB; Group 2: Peripheral nerve stimulation-guided (ENP) BPB. Under general anesthesia, axillary BPB was performed, under lidocaine 2% without vasoconstrictor injection at the maximum dose of 0,7ml.kg-1. The motor latency, motor block and volume difference between the BPB between the techniques were evaluated by recording motor action potentials compounds of the radial nerve. The measurement of skin temperature (ST), by infrared thermography, was carried out in areas of interest, previously stipulated in the forepaw, digits and forearm, in order to verify the correlation of the variation in the effectiveness of BPB. In 92.5% of the animals the nerves resulting consisted of connections between the ventral rami of the last four cervical spinal nerves (C5, C6, C7, C8) and first thoracic (T1). No significant difference was observed in time performance of US/ENP-guided BPB (4.3 ? 0.73 min) or ENP-guided BPB (6.4 ? 0.68 minutes), however, significantly less volume administration was necessary to local anesthetic in the US/ENP-guided BPB (0.61 ? 0.15 mL vs 1.22 ? 0.17; P <0.0001). Despite the lower volume used, it was observed that the US/ENP group had shorter on set time block (1.1 ? 0.45) compared to ENP group (1.95 ? 0.79; P <0.01). The US/ENP-guided BPB or ENP-guided BPB resulted in a substantial and significant increase in ST areas of interest in the radial, musculocutaneous, median and ulnar nerves (P < 0.001), however this increase was outstanding in hand and digits regions Thus it is concluded that the US/ENP-guided BPB is an effective and easy technique to reproduce in this experimental model which required lower volume of local anesthetic, provided a smaller motor latency and a higher motor blockade time when compared to the ENP-guided BPB. The increase in ST is a highly effective tool for assessing the effectiveness of BPB with predictive value, sensitivity and specificity of 100%. Furthermore, future clinical studies are needed to verify its correlation with the anaesthetized area.
publishDate 2016
dc.date.issued.fl_str_mv 2016-04-28
dc.date.accessioned.fl_str_mv 2017-03-22T12:11:48Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/doctoralThesis
format doctoralThesis
status_str publishedVersion
dc.identifier.citation.fl_str_mv MOREIRA, Rodrigo Mencalha. Avalia??o morfol?gica, eletromiogr?fica e por termografia infravermelha do bloqueio do plexo braquial em coelhos guiado por ultrassonografia. 2016. 79 f. Tese (Doutorado em Medicina Veterin?ria, Patologia e Ci?ncias Cl?nicas) - Instituto de Veterin?ria, Universidade Federal Rural do Rio de Janeiro, Serop?dica - RJ, 2016.
dc.identifier.uri.fl_str_mv https://tede.ufrrj.br/jspui/handle/jspui/1473
identifier_str_mv MOREIRA, Rodrigo Mencalha. Avalia??o morfol?gica, eletromiogr?fica e por termografia infravermelha do bloqueio do plexo braquial em coelhos guiado por ultrassonografia. 2016. 79 f. Tese (Doutorado em Medicina Veterin?ria, Patologia e Ci?ncias Cl?nicas) - Instituto de Veterin?ria, Universidade Federal Rural do Rio de Janeiro, Serop?dica - RJ, 2016.
url https://tede.ufrrj.br/jspui/handle/jspui/1473
dc.language.iso.fl_str_mv por
language por
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