Microbiota bacteriana em mi?ases umbilicais por larvas de Cochliomyia hominivorax Coquerel, 1858 (Diptera: Calliphoridae) em bezerros naturalmente infestados

Detalhes bibliográficos
Autor(a) principal: Oliveira, Carlos C?sar Mascarenhas da Silva de
Data de Publicação: 2006
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Biblioteca Digital de Teses e Dissertações da UFRRJ
Texto Completo: https://tede.ufrrj.br/jspui/handle/tede/788
Resumo: The objective of this study was to identify the bacterial microbiota in calf navels myiasis caused by Cochliomyia hominivorax larvae. Two places were choosed to colect the material: the Nhumirim ranch belonging to Embrapa Pantanal, Corumb?-MS and the Santo Ant?nio ranch, Miguel Pereira-RJ. There were collected 62 cotton swabs from calf navels naturally caused by C. hominivorax larvae. After that, the cotton swabs were sent to the Veterinary Bacteriology Laboratory at UFRRJ inside carriage test tubes with agar. The primary isolation used bovine blood agar and brain heart infusion (BHI) agar. After the incubation period, the isolates were evaluated by means of Gram staining, catalase testing and 30% KOH. After stained, the isolates were evaluated by their stain morphologic and color characteristics. Species of Staphylococcus spp. were streaked in selectives agar to Staphylococcus spp. for the species identication and was used coagulase test, nitrate reduction test, VP, urease production and sugar fermentation. To Streptococcus spp. was used selective agar to Streptococcus spp. species througout inoculation in metilene blue milk, esculine test and hipurate test. The specimens of the family Enterobacteriaceae used THI test, motility in tube test, indol production test, gelatine production, urease production, citrate and malonate degradation. A total of 89 colonies were isolated; 14 genera and five families were identified. The most frequent family was Enterobacteriaceae and the genera more frequent was Bacillus and Micrococcus. Pathogenic species were isolated, but no secondary infection ocurred in the calves; some antibacterial or bacteriostatic substances may be produced by larvae of C. hominivorax or bacterial microbiota of the myiasis of the navel.
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spelling Sanavria, Argemiro253.991.280-32http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4780325D8Souza, Miliane Moreira Soares de010.761.987-32http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4701812Y2079.223.607-62http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4744348Z3Oliveira, Carlos C?sar Mascarenhas da Silva de2016-04-28T20:15:36Z2006-11-102006-03-14OLIVEIRA, Carlos C?sar Mascarenhas da Silva de. Microbiota bacteriana em mi?ases umbilicais por larvas de Cochliomyia hominivorax Coquerel, 1858 (Diptera: Calliphoridae) em bezerros naturalmente infestados. 2006. 28 f. Disserta??o (Mestrado em Ci?ncias Veterin?rias) - Instituto de Veterin?ria, Universidade Federal Rural do Rio de Janeiro, Serop?dica - RJ, 2006.https://tede.ufrrj.br/jspui/handle/tede/788The objective of this study was to identify the bacterial microbiota in calf navels myiasis caused by Cochliomyia hominivorax larvae. Two places were choosed to colect the material: the Nhumirim ranch belonging to Embrapa Pantanal, Corumb?-MS and the Santo Ant?nio ranch, Miguel Pereira-RJ. There were collected 62 cotton swabs from calf navels naturally caused by C. hominivorax larvae. After that, the cotton swabs were sent to the Veterinary Bacteriology Laboratory at UFRRJ inside carriage test tubes with agar. The primary isolation used bovine blood agar and brain heart infusion (BHI) agar. After the incubation period, the isolates were evaluated by means of Gram staining, catalase testing and 30% KOH. After stained, the isolates were evaluated by their stain morphologic and color characteristics. Species of Staphylococcus spp. were streaked in selectives agar to Staphylococcus spp. for the species identication and was used coagulase test, nitrate reduction test, VP, urease production and sugar fermentation. To Streptococcus spp. was used selective agar to Streptococcus spp. species througout inoculation in metilene blue milk, esculine test and hipurate test. The specimens of the family Enterobacteriaceae used THI test, motility in tube test, indol production test, gelatine production, urease production, citrate and malonate degradation. A total of 89 colonies were isolated; 14 genera and five families were identified. The most frequent family was Enterobacteriaceae and the genera more frequent was Bacillus and Micrococcus. Pathogenic species were isolated, but no secondary infection ocurred in the calves; some antibacterial or bacteriostatic substances may be produced by larvae of C. hominivorax or bacterial microbiota of the myiasis of the navel.O objetivo deste trabalho foi identificar a microbiota bacteriana de mi?ases umbilicais de bezerros rec?m-nascidos. Dois locais foram escolhidos para coleta de material: a Fazenda Nhumirim pertencente ? Embrapa Pantanal-MS e a Fazenda Santo Ant?nio, no munic?pio de Miguel Pereira-RJ. Amostras do exsudato de les?es por larvas de Cochliomyia hominivorax foram coletados com swabs em 62 bezerros, acondicionadas em tubos de ensaio contendo meio de transporte e encaminhadas ao Laborat?rio de Bacteriologia Veterin?ria da UFRRJ. No isolamento prim?rio foi utilizado ?gar sangue ou ?gar infus?o de c?rebro e cora??o. As col?nias isoladas foram submetidas ? colora??o pelo m?todo de Gram, teste da catalase e hidr?xido de pot?ssio a 3%. De acordo com as caracter?sticas morfotintoriais e de crescimento, os isolados foram processados para melhor identifica??o. Para a identifica??o das esp?cies de Staphylococcus spp.; as col?nias isoladas foram semeadas em meios seletivos, para observa??o dos aspectos fenot?picos caracter?sticos do g?nero. A identifica??o foi efetuada por meio do procedimento padr?o: prova da coagulase livre e ligada, provas de redu??o de nitratos, VP, produ??o de urease e fermenta??o de a??cares. A identifica??o dos isolados de Streptococcus spp foi efetuada atrav?s do repique em meio seletivo e, posteriormente, atrav?s da inocula??o em leite adicionado com azul de metileno, e das provas de hidr?lise da esculina e do hipurato.Para as amostras suspeitas de enterobact?rias, foi utilizado o meio MacConkey no isolamento prim?rio e as seguintes provas de identifica??o: comportamento em ?gar tr?plice a??car-ferro, motilidade em tubo, produ??o do indol, produ??o de ?cidos a partir da glicose, fermenta??o de a??cares, redu??o do nitrato, produ??o de gelatinase, produ??o de urease, degrada??o do citrato e do malonato. Das amostras coletadas, foram isoladas 89 col?nias, representando 14 g?neros diferentes, de cinco fam?lias . A fam?lia mais freq?ente foi a Bacillaceae; sendo os g?neros mais prevalentes o Bacillus e o Micrococcus. Esp?cies patog?nicas foram isoladas, mas infec??o bacteriana n?o foi percebida nos bezerros do experimento; indicando que alguma subst?ncia bactericida ou bacteriost?tica seja produzida pelas larvas ou por alguma bact?ria da microbiota umbilical.Made available in DSpace on 2016-04-28T20:15:36Z (GMT). No. of bitstreams: 1 2006 - Carlos C?sar Mascarenhas da Silva de Oliveira.pdf: 2632878 bytes, checksum: d43615ce0a787516f24b8ef5193ca4a6 (MD5) Previous issue date: 2006-03-14Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico - CNPqapplication/pdfhttps://tede.ufrrj.br/retrieve/59763/2006%20-%20Carlos%20C%c3%a9sar%20Mascarenhas%20da%20Silva%20de%20Oliveira.pdf.jpgporUniversidade Federal Rural do Rio de JaneiroPrograma de P?s-Gradua??o em Ci?ncias Veterin?riasUFRRJBrasilInstituto de Veterin?riaCochliomyia hominivoraxmi?asebovinosmyiasiscattleParasitologiaMicrobiota bacteriana em mi?ases umbilicais por larvas de Cochliomyia hominivorax Coquerel, 1858 (Diptera: Calliphoridae) em bezerros naturalmente infestadosBacterial microbiota in calf navels myiasis by Cochliomyia hominivorax Coquerel, 1858 (Calliphoridae) larvaeinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UFRRJinstname:Universidade Federal Rural do Rio de Janeiro (UFRRJ)instacron:UFRRJTHUMBNAIL2006 - Carlos C?sar Mascarenhas da Silva de Oliveira.pdf.jpg2006 - Carlos C?sar Mascarenhas da Silva de Oliveira.pdf.jpgimage/jpeg4028http://localhost:8080/tede/bitstream/tede/788/3/2006+-+Carlos+C%C3%A9sar+Mascarenhas+da+Silva+de+Oliveira.pdf.jpg38c41878569d340a8180f52a1fa147e6MD53TEXT2006 - Carlos C?sar Mascarenhas da Silva de Oliveira.pdf.txt2006 - Carlos C?sar Mascarenhas da Silva de Oliveira.pdf.txttext/plain69270http://localhost:8080/tede/bitstream/tede/788/2/2006+-+Carlos+C%C3%A9sar+Mascarenhas+da+Silva+de+Oliveira.pdf.txt251fa64ec21cf52966dbacd5079e1081MD52ORIGINAL2006 - Carlos C?sar Mascarenhas da Silva de Oliveira.pdf2006 - Carlos C?sar Mascarenhas da Silva de Oliveira.pdfapplication/pdf2642956http://localhost:8080/tede/bitstream/tede/788/1/2006+-+Carlos+C%C3%A9sar+Mascarenhas+da+Silva+de+Oliveira.pdf1caf56469939856936d91d71b53547d7MD51tede/7882022-06-09 09:41:30.831oai:localhost:tede/788Biblioteca Digital de Teses e Dissertaçõeshttps://tede.ufrrj.br/PUBhttps://tede.ufrrj.br/oai/requestbibliot@ufrrj.br||bibliot@ufrrj.bropendoar:2022-06-09T12:41:30Biblioteca Digital de Teses e Dissertações da UFRRJ - Universidade Federal Rural do Rio de Janeiro (UFRRJ)false
dc.title.por.fl_str_mv Microbiota bacteriana em mi?ases umbilicais por larvas de Cochliomyia hominivorax Coquerel, 1858 (Diptera: Calliphoridae) em bezerros naturalmente infestados
dc.title.alternative.eng.fl_str_mv Bacterial microbiota in calf navels myiasis by Cochliomyia hominivorax Coquerel, 1858 (Calliphoridae) larvae
title Microbiota bacteriana em mi?ases umbilicais por larvas de Cochliomyia hominivorax Coquerel, 1858 (Diptera: Calliphoridae) em bezerros naturalmente infestados
spellingShingle Microbiota bacteriana em mi?ases umbilicais por larvas de Cochliomyia hominivorax Coquerel, 1858 (Diptera: Calliphoridae) em bezerros naturalmente infestados
Oliveira, Carlos C?sar Mascarenhas da Silva de
Cochliomyia hominivorax
mi?ase
bovinos
myiasis
cattle
Parasitologia
title_short Microbiota bacteriana em mi?ases umbilicais por larvas de Cochliomyia hominivorax Coquerel, 1858 (Diptera: Calliphoridae) em bezerros naturalmente infestados
title_full Microbiota bacteriana em mi?ases umbilicais por larvas de Cochliomyia hominivorax Coquerel, 1858 (Diptera: Calliphoridae) em bezerros naturalmente infestados
title_fullStr Microbiota bacteriana em mi?ases umbilicais por larvas de Cochliomyia hominivorax Coquerel, 1858 (Diptera: Calliphoridae) em bezerros naturalmente infestados
title_full_unstemmed Microbiota bacteriana em mi?ases umbilicais por larvas de Cochliomyia hominivorax Coquerel, 1858 (Diptera: Calliphoridae) em bezerros naturalmente infestados
title_sort Microbiota bacteriana em mi?ases umbilicais por larvas de Cochliomyia hominivorax Coquerel, 1858 (Diptera: Calliphoridae) em bezerros naturalmente infestados
author Oliveira, Carlos C?sar Mascarenhas da Silva de
author_facet Oliveira, Carlos C?sar Mascarenhas da Silva de
author_role author
dc.contributor.advisor1.fl_str_mv Sanavria, Argemiro
dc.contributor.advisor1ID.fl_str_mv 253.991.280-32
dc.contributor.advisor1Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4780325D8
dc.contributor.advisor-co1.fl_str_mv Souza, Miliane Moreira Soares de
dc.contributor.advisor-co1ID.fl_str_mv 010.761.987-32
dc.contributor.advisor-co1Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4701812Y2
dc.contributor.authorID.fl_str_mv 079.223.607-62
dc.contributor.authorLattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4744348Z3
dc.contributor.author.fl_str_mv Oliveira, Carlos C?sar Mascarenhas da Silva de
contributor_str_mv Sanavria, Argemiro
Souza, Miliane Moreira Soares de
dc.subject.por.fl_str_mv Cochliomyia hominivorax
mi?ase
bovinos
topic Cochliomyia hominivorax
mi?ase
bovinos
myiasis
cattle
Parasitologia
dc.subject.eng.fl_str_mv myiasis
cattle
dc.subject.cnpq.fl_str_mv Parasitologia
description The objective of this study was to identify the bacterial microbiota in calf navels myiasis caused by Cochliomyia hominivorax larvae. Two places were choosed to colect the material: the Nhumirim ranch belonging to Embrapa Pantanal, Corumb?-MS and the Santo Ant?nio ranch, Miguel Pereira-RJ. There were collected 62 cotton swabs from calf navels naturally caused by C. hominivorax larvae. After that, the cotton swabs were sent to the Veterinary Bacteriology Laboratory at UFRRJ inside carriage test tubes with agar. The primary isolation used bovine blood agar and brain heart infusion (BHI) agar. After the incubation period, the isolates were evaluated by means of Gram staining, catalase testing and 30% KOH. After stained, the isolates were evaluated by their stain morphologic and color characteristics. Species of Staphylococcus spp. were streaked in selectives agar to Staphylococcus spp. for the species identication and was used coagulase test, nitrate reduction test, VP, urease production and sugar fermentation. To Streptococcus spp. was used selective agar to Streptococcus spp. species througout inoculation in metilene blue milk, esculine test and hipurate test. The specimens of the family Enterobacteriaceae used THI test, motility in tube test, indol production test, gelatine production, urease production, citrate and malonate degradation. A total of 89 colonies were isolated; 14 genera and five families were identified. The most frequent family was Enterobacteriaceae and the genera more frequent was Bacillus and Micrococcus. Pathogenic species were isolated, but no secondary infection ocurred in the calves; some antibacterial or bacteriostatic substances may be produced by larvae of C. hominivorax or bacterial microbiota of the myiasis of the navel.
publishDate 2006
dc.date.available.fl_str_mv 2006-11-10
dc.date.issued.fl_str_mv 2006-03-14
dc.date.accessioned.fl_str_mv 2016-04-28T20:15:36Z
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dc.identifier.citation.fl_str_mv OLIVEIRA, Carlos C?sar Mascarenhas da Silva de. Microbiota bacteriana em mi?ases umbilicais por larvas de Cochliomyia hominivorax Coquerel, 1858 (Diptera: Calliphoridae) em bezerros naturalmente infestados. 2006. 28 f. Disserta??o (Mestrado em Ci?ncias Veterin?rias) - Instituto de Veterin?ria, Universidade Federal Rural do Rio de Janeiro, Serop?dica - RJ, 2006.
dc.identifier.uri.fl_str_mv https://tede.ufrrj.br/jspui/handle/tede/788
identifier_str_mv OLIVEIRA, Carlos C?sar Mascarenhas da Silva de. Microbiota bacteriana em mi?ases umbilicais por larvas de Cochliomyia hominivorax Coquerel, 1858 (Diptera: Calliphoridae) em bezerros naturalmente infestados. 2006. 28 f. Disserta??o (Mestrado em Ci?ncias Veterin?rias) - Instituto de Veterin?ria, Universidade Federal Rural do Rio de Janeiro, Serop?dica - RJ, 2006.
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