Avalia??o dos Perfis Prot?ico e Lip?dico na Resposta de Rhipicephalus Microplus ? Infec??o com Fungos.

Detalhes bibliográficos
Autor(a) principal: Angelo, Isabele da Costa
Data de Publicação: 2011
Tipo de documento: Tese
Idioma: por
Título da fonte: Biblioteca Digital de Teses e Dissertações da UFRRJ
Texto Completo: https://tede.ufrrj.br/jspui/handle/jspui/1073
Resumo: The study evaluated the protein and lipid profiles of Rhipicephalus microplus engorged females after infection by Metarhizium anisopliae, Beauveria bassiana and Fusarium oxysporum. The treatments were immersion or inoculation of conidial suspension in R. microplus. The hemolymph was collected 24 and 48 hours after treatment. The cell-free hemolymph was separated of hemocytes by centrifugation and hemocytes resuspended in phosphate buffer pH 7.2. The amount of total protein was determined in both fractions of hemolymph and hemocytes were quantified. The cell-free hemolymph was filtered through a 100 kDa and 10 kDa membranes, and analyzed by electrophoresis and liquid chromatography (HPLC). The proteome of cell-free hemolymph (treatment by injection) was evaluated by 2DPAGE. Changes were observed in amount total protein and the amount of hemocytes, but no difference was observed in the electrophoretic profile (1D-PAGE) of the cell-free hemolymph. In haemocytes, the entomopathogens reduced the amount of serpins, while F. oxysporum caused increased. In 2D-PAGE variations were observed in both expression and presence/absence of protein between the groups. The cell-free hemolymph antimicrobial activity was tested against Escherichia coli and Staphylococcus aureus and against the fungus used in the treatment of engorged ticks. The hemolymph collected 48 hours after the immersion treatment with B. bassiana apresented activity anti-B. bassiana with 48 hours of evaluation. This hemolymph was subjected to Superose column to HPLC and peak was collected and analyzed on the analytical column C18. The fractions were collected from the C18 and its apresented activity anti-B. bassiana, but showed no activity against Candida albicans. These fractions were analyzed by Maldi-Tof and most of them had in common an ion with m/z 1,119.5; however, other ions may be involved with this activity antimicrobial. The lipids present in cell-free hemolymph, in the hemocyte and fat body were extracted and analyzed by thin layer chromatography (TLC) or HPTLC for neutral lipids and phospholipids. The classes of neutral lipids in the cell-free hemolymph were cholesterol ester, cholesterol (CHO) and fatty acids (FA), which have varied depending on the fungus used, type of treatment and observation time. Phospholipids found were phosphatidylcholine and phosphatidylethanolamine, its were not significantly altered after the fungal infection. In hemocytes, the same classes of lipids were found and B. bassiana modified phospholipids, while M. anisopliae s.l. altered FA and CHO. The fat body showed, in addition to these classes of neutral lipids, the triglycerides, which increased significantly 48 hours after inoculation with M. anisopliae s.l. The lipase activity in fat body was measured and it was demonstrated that increased activity 48 hours after inoculation, mainly in the group inoculated with Metarhizium. Therefore, the results showed alterations related to the proteins expression in the hemocytes and the cell-free hemolymph after inoculation with fungi, immunosuppression of hemocytes and antimicrobial peptides induction after infection with B. bassiana, besides changes in the lipid profile of R. microplus after infection. However, further studies are necessary to understand these changes.
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spelling Bittencourt, V?nia Rita Elias Pinheiro50519867653http://lattes.cnpq.br/3888832724995864Chagas, Evelize Folly dasChagas, Evelize Folly dasFernandes, Everton Kort KampAtella, Georgia CorreaSilva, M?rcia Regina Soares daOliveira, Pedro Lagerblad de9034779718http://lattes.cnpq.br/5028095543336052Angelo, Isabele da Costa2016-06-09T16:27:32Z2011-03-03Angelo, Isabele da Costa. Avalia??o dos Perfis Prot?ico e Lip?dico na Resposta de Rhipicephalus Microplus ? Infec??o com Fungos.. 2011. [143 folhas]. Tese( PROGRAMA DE P?S-GRADUA??O EM ADMINISTRA??O) - Universidade Federal Rural do Rio de Janeiro, [Serop?dica/RJ] .https://tede.ufrrj.br/jspui/handle/jspui/1073The study evaluated the protein and lipid profiles of Rhipicephalus microplus engorged females after infection by Metarhizium anisopliae, Beauveria bassiana and Fusarium oxysporum. The treatments were immersion or inoculation of conidial suspension in R. microplus. The hemolymph was collected 24 and 48 hours after treatment. The cell-free hemolymph was separated of hemocytes by centrifugation and hemocytes resuspended in phosphate buffer pH 7.2. The amount of total protein was determined in both fractions of hemolymph and hemocytes were quantified. The cell-free hemolymph was filtered through a 100 kDa and 10 kDa membranes, and analyzed by electrophoresis and liquid chromatography (HPLC). The proteome of cell-free hemolymph (treatment by injection) was evaluated by 2DPAGE. Changes were observed in amount total protein and the amount of hemocytes, but no difference was observed in the electrophoretic profile (1D-PAGE) of the cell-free hemolymph. In haemocytes, the entomopathogens reduced the amount of serpins, while F. oxysporum caused increased. In 2D-PAGE variations were observed in both expression and presence/absence of protein between the groups. The cell-free hemolymph antimicrobial activity was tested against Escherichia coli and Staphylococcus aureus and against the fungus used in the treatment of engorged ticks. The hemolymph collected 48 hours after the immersion treatment with B. bassiana apresented activity anti-B. bassiana with 48 hours of evaluation. This hemolymph was subjected to Superose column to HPLC and peak was collected and analyzed on the analytical column C18. The fractions were collected from the C18 and its apresented activity anti-B. bassiana, but showed no activity against Candida albicans. These fractions were analyzed by Maldi-Tof and most of them had in common an ion with m/z 1,119.5; however, other ions may be involved with this activity antimicrobial. The lipids present in cell-free hemolymph, in the hemocyte and fat body were extracted and analyzed by thin layer chromatography (TLC) or HPTLC for neutral lipids and phospholipids. The classes of neutral lipids in the cell-free hemolymph were cholesterol ester, cholesterol (CHO) and fatty acids (FA), which have varied depending on the fungus used, type of treatment and observation time. Phospholipids found were phosphatidylcholine and phosphatidylethanolamine, its were not significantly altered after the fungal infection. In hemocytes, the same classes of lipids were found and B. bassiana modified phospholipids, while M. anisopliae s.l. altered FA and CHO. The fat body showed, in addition to these classes of neutral lipids, the triglycerides, which increased significantly 48 hours after inoculation with M. anisopliae s.l. The lipase activity in fat body was measured and it was demonstrated that increased activity 48 hours after inoculation, mainly in the group inoculated with Metarhizium. Therefore, the results showed alterations related to the proteins expression in the hemocytes and the cell-free hemolymph after inoculation with fungi, immunosuppression of hemocytes and antimicrobial peptides induction after infection with B. bassiana, besides changes in the lipid profile of R. microplus after infection. However, further studies are necessary to understand these changes.O trabalho avaliou os perfis prot?ico e lip?dico de f?meas ingurgitadas de Rhipicephalus microplus ap?s infec??o com Metarhizium anisopliae s.l., Beauveria bassiana ou Fusarium oxysporum. Os tratamentos foram imers?o ou inocula??o da suspens?o conidial em R. microplus. A hemolinfa foi coletada 24 e 48 horas ap?s os tratamentos. O plasma foi separado dos hem?citos por centrifuga??o e os hem?citos resuspensos em tamp?o fosfato pH 7,2. A concentra??o de prote?na total foi determinada em ambas as fra??es da hemolinfa e os hem?citos quantificados. O plasma da hemolinfa foi filtrado em membrana de 100 kDa e 10 kDa, sendo analisados por eletroforese e cromatografia l?quida de alta efici?ncia (CLAE). O proteoma do plasma da hemolinfa (tratamento por inocula??o) foi avaliado por gel 2D. Foram observadas varia??es na quantidade de prote?na total de ambas as fra??es da hemolinfa, na quantidade de hem?citos bem como na intensidade de prote?nas/pept?deos expressos no plasma da hemolinfa. Nos hem?citos, os entomopat?genos reduziram a quantidade de serpinas, enquanto F. oxysporum causou aumento. No gel 2D foram observadas varia??es na express?o bem como na aus?ncia/presen?a de prote?nas entre os grupos. O plasma da hemolinfa teve sua atividade antimicrobiana testada contra Escherichia coli e Staphylococcus aureus e contra o fungo utilizado no tratamento das f?meas ingurgitadas. A hemolinfa coletada 48 horas ap?s o tratamento por imers?o com B. bassiana apresentou atividade anti-B. bassiana com 48 horas de avalia??o. Esta hemolinfa foi submetida ? coluna Superose de CLAE e o pico coletado analisado na coluna anal?tica C18. As fra??es coletadas da C18 apresentaram atividade anti-B. bassiana, por?m n?o apresentaram atividade contra Candida albicans. Essas fra??es foram analisadas por Maldi-Tof e a maioria delas apresentou um ?on com raz?o m/z 1.119,5; no entanto, outros ?ons podem estar envolvidos com essa atividade antimicrobiana. Os lip?deos presentes no plasma da hemolinfa, nos hem?citos e no corpo gorduroso foram extra?dos e analisados por cromatografia em camada delgada (CCD) ou CCD de alta performance para lip?deos neutros e fosfolip?deos. As classes de lip?deos neutros encontradas no plasma da hemolinfa foram colesterol-?ster, colesterol (CHO) e ?cidos graxos (AG), que sofreram altera??es em fun??o do fungo utilizado, tipo de tratamento e tempo de observa??o. Os fosfolip?deos encontrados foram fosfatidilcolina e fosfatidiletanolamina, que n?o foram significativamente alterados ap?s a infec??o f?ngica. Nos hem?citos, as mesmas classes de lip?deos foram encontradas e B. bassiana alterou os fosfolip?deos, enquanto M. anisopliae s.l. alterou os AG e CHO. O corpo gorduroso apresentou, al?m destas classes de lip?deos neutros, o triacilglicerol, que aumentou significativamente 48 horas ap?s a inocula??o com M. anisopliae s.l. A atividade lipase no corpo gorduroso foi mensurada, sendo evidenciado um aumento 48 horas ap?s a inocula??o, principalmente no grupo inoculado com Metarhizium. Portanto, os resultados demonstraram altera??es na express?o de prote?nas no plasma da hemolinfa e nos hem?citos ap?s inocula??o com os fungos, imunossupress?o dos hem?citos, indu??o de pept?deos com atividade antimicrobiana ap?s infec??o com B. bassiana, al?m de altera??es no perfil lip?dico de R. microplus ap?s infec??o. No entanto, maiores estudos s?o necess?rios para o entendimento dessas altera??es.Submitted by Sandra Pereira (srpereira@ufrrj.br) on 2016-06-09T16:27:04Z No. of bitstreams: 1 ISABELE DA COSTA ANGELO.pdf: 4140152 bytes, checksum: bbd35489ea24b040be77df36cd797987 (MD5)Made available in DSpace on 2016-06-09T16:27:32Z (GMT). No. of bitstreams: 1 ISABELE DA COSTA ANGELO.pdf: 4140152 bytes, checksum: bbd35489ea24b040be77df36cd797987 (MD5) Previous issue date: 2011-03-03Coordena??o de Aperfei?oamento de Pessoal de N?vel Superiorapplication/pdfhttps://tede.ufrrj.br/retrieve/4599/ISABELE%20DA%20COSTA%20ANGELO.pdf.jpgporUniversidade Federal Rural do Rio de JaneiroPrograma de P?s-Gradua??o em Ci?ncias Veterin?riasUFRRJBrasilInstituto de Veterin?riaALVES, S.B. Controle Microbiano de Insetos. 2? ed. Piracicaba: FEALQ, 1998. 1163p. ANGELO, I.C. Avalia??o in vitro dos efeitos de Isaria farinosa, I. fumosorosea, Paecilomyces lilacinus e Lecanicillium lecanii sobre Boophilus microplus. Disserta??o de Mestrado. 37p, 2007. ANGELO, I.C.; FERNANDES, E.K.K.; BAHIENSE, T.C.; PERINOTTO, W.M.S.; MORAES, A.P.R.; TERRA, A.L.M.; BITTENCOURT, V.R.E.P. Efficiency of Lecanicillium lecanii to control the tick Rhipicephalus microplus. Veterinary Parasitology, v.172, p.317- 322, 2010. ARMSTRONG, P.B. The contribution of proteinase inhibitors to immune defense. Trends in Immunology, v.22, p.47-52, 2001. ARRESE, E.L.; HOWARD, A.D.; PATEL, R.T.; RIMOLDI, O.J.; SOULAGES, J.L. Mobilization of lipid stores in Manduca sexta: cDNA cloning and developmental expression of fat body triglyceride lipase, TGL. Insect Biochemistry and Molecular Biology, v.40, p.91-99, 2010. ARRESE, E.L.; SOULAGES, J.L. Insect fat body: energy, metabolism, and regulation. Annual Review of Entomology, v.55, p.207-225, 2010. ASO, Y.; YAMASHITA, T.; MENO, K.; MURAKAMI, M. Inhibition of prophenoloxidaseactivating enzyme from Bombyx mori by endogenous chymotrypsin inhibitors. Biochemistry and Molecular Biology International, v.33, p.751?758, 1994. ATHENSTAEDT, K.; DAUM, G. The life cycle of neutral lipids: synthesis, storage and degradation. Cellular and Molecular Life Sciences, v.63, p.1355-1369, 2006 AUERSWALD, L.; SIEGERT, K.J.; G?DE, G. Activation of triacylglycerol lipase in the fat body of a beetle by adipokinetic hormone. Insect Biochemistry and Molecular Biology, v.35, p.461-470, 2005. AZZOLINI, S.S.; SANTOS, J.M.C.; SOUZA, A.F.; TORQUATO, R.J.S.; HIRATA, I.Y.; ANDREOTTI, R.; TANAKA, A.S. Purification, characterization, and cloning of a serine proteinase inhibitor from the ectoparasite Haematobia irritans irritans (Diptera: Muscidae). Experimental Parasitology, v.106, n.3-4, p.103-109, 2004. BACH?RE, E.; GUEGUEN, Y.; GONZALEZ, M.; LORGERIL, J.; GARNIER, J.; ROMESTAND, B. Insights into the anti-microbial defense of marine invertebrates: the penaeid shrimps and the oyster Crassostrea gigas. Immunological Reviews, v.198, p.149? 168, 2004. BAHIENSE, T. C.; FERNANDES, ?. K. K.; ANGELO, I. C.; PERINOTTO, W. M. S. and BITTENCOURT, V. R. E. P. Performance of Metarhizium anisopliae and its combination with deltamethrin against a pyrethroid-resistant strain of Boophilus microplus in a stall test. Annals of the New York Academy of Sciences, v.1149, p.242-245, 2008. BALASHOV, Yu.S. 1983. An Atlas of Ixodid Tick Ultrastructure. In: Raikhel, A.S., Hoogstral, I.L. (Ed.), Springer, Lanham. 93 BANDSTRA, S.R.; FRIED, B.; SHERMA, J. High-performance thin-layer chromatographic analysis of neutral lipids and phospholipids in Biomphalaria glabrata patently infected with Echinostoma caproni. Parasitology Research, v.99, p.414?418, 2006. BARBAULT, F.; LANDON, C.; GUENNEUGUES, M.; MEYER, J.P.; SCHOTT, V.; DIMARCQ, J.L.; VOVELLE, F. Solution structure of Alo-3: a new knottin-type antifungal peptide from the insect Acrocinus longimanus. Biochemistry, v.42, p.14434-14442, 2003. BARROS, T.A.M.; EVANS, D.E. A??o de gram?neas forrageiras em larvas infectantes do carrapato dos bovinos Boophilus microplus. Pesquisa Veterin?ria Brasileira, v.9, p.17-21, 1989. BEENAKKERS, A.M.T.; VAN DER HORST, D.J.; VANMARREWIJK, W.J.A. Insect flight metabolism. Insect Biochemistry, v.14, p.243-260, 1984. BEISSON, F.; TISS, A.; RIVI?RE, C.; VERGER, R. Methods for lipase detection and assay: a critical review. European Journal of Lipid Science and Technology, v.2, 133-153, 2000. BIDOCHKA, M.J.; SMALL, C.L.; SPIRONELLO, M. Recombination within sympatric cryptic species of the insect pathogenic fungus Metarhizium anisopliae. Environmental Microbiology, v.7, p.1361-1368, 2005. BINNINGTON, K.C; OBENCHAIN, F.D. Structure and function of the circulatory, nervous, and neuroendocrine systems of ticks. In: Obenchain, F.D. and Galun R. (Eds) Physiology of Ticks. Pergamon Press, Oxford, 1982. BISCHOFF, J.F.; REHNER, S.A.; HUMBER, R.A. Metarhizium frigidum sp. nov.: a cryptic species of M. anisopliae and a member of the M. flavoviride complex. Mycologia, v.98, p.737-745, 2006. BISCHOFF, J.F.; REHNER, S.A.; HUMBER, R.A. A multilocus phylogeny of the Metarhizium anisopliae lineage. Mycologia, v.101, p.512-530, 2009. BITTENCOURT, V.R.E.P.; MASCARENHAS, A.G.; FACCINI, J.L.H. Mecanismo de infec??o do fungo Metarhizium anisopliae no carrapato Boophilus microplus em condi??es experimentais. Ci?ncia Rural, Santa Maria, v.29, n.2, p.351-354, 1999. BITTENCOURT, V.R.E.P.; MASSARD, C.L.; LIMA, A.F. Din?mica da infec??o do fungo Metarhizium anisopliae (Metschinikoff, 1879) Sorokin, 1883, sobre o carrapato Boophilus microplus (Canestrini, 1887). Revista Universidade Rural ? S?rie Ci?ncias da Vida, v.17, p.83-88, 1995. BRASAEMLE, D.L. Thematic review series: adipocyte biology. The perilipin family of structural lipid droplet proteins: stabilization of lipid droplets and control of lipolysis. Journal of Lipid Research, v.48, p.2547-2559, 2007. BRAZ, G.R.C.; COELHO, H.S.L.; MASUDA, H.; OLIVEIRA, P.L. A missing metabolic pathway in the cattle tick Boophilus microplus. Current Biology, v.9, n.13, p.703-706, 1999. BROWNBRIDGE, M.; COSTA, S.; JARONSKI, S.T. Effects of in vitro passage of Beauveria bassiana on virulence to Bemisia argentifolii. Journal of Invertebrate Pathology, v.77, p.280-283, 2001. BULET, P.; CHARLET, M.; HETRU, C. In Innate Immunity, (Ezekowitz, R.A.B. and Hoffmann, J.A., Eds.) pp. 89-107. Humana Press, Totowa, NJ. 2003. BULET, P.; HETRU, C.; DIMARQ, J.L.; HOFFMANN, D. Antimicrobial peptides in insects; structure and function. Developmental and Comparative Immunology, v.23, p.329?344, 1999. 94 BULET, P.; STOCKLIN, R. Insect antimicrobial peptides: structure, properties and gene regulation. Protein and Peptide Letters, v.12, p.3-11, 2005. CABRAL, G.A. Lipids as bioeffectors in the immune system. Life Sciences, v.77, p.1699? 1710, 2005. CANAVOSO, L. E.; JOUNI, Z.E.; KARNAS, K.J. PENNINGTON, J.E.; WELLS, M.A. Fat metabolism in insects. Annual Review of Nutrition, v.21, p.23-46, 2001. CASTRILLO, L.A.; ROBERTS, D.W.; VANDENBERG, J.D. The fungal past, present, and future: germination, ramification, and reproduction. Journal of Invertebrate Pathology, v.89, p.46-56, 2005. CASTRO-OCHOA, L.D.; RODRIGUEZ-G?MEZ, C.; VALERIO-ALFARO, G.; ROS, R.O. Screening, purification and characterization of the thermoalkalophilic lipase produced by Bacillus thermoleovorans CCR11. Enzyme and Microbial Technology, v.37, p.648-654, 2005. CERAUL, S.M.; DREHER-LESNICK, S.M.; GILLESPIE, J.J.; RAHMAN, M.S.; AZAD, A.F. New tick defensin isoform and antimicrobial gene expression in response to Rickettsia montanensis challenge. Infection and Immunity, v.75, p.1973?1983, 2007. CHANDLER, D.; DAVIDSON, G.; PELL, J.K.; BALL, B.V.; SHAW, K.; SUNDERLAND, K.D. Fungal biocontrol of acari. Biocontrol Science and Technology, v.10, p.357-384, 2000. CHENG, T.C.; RODRICH, G.E.; FOLEY, D.A.; KOEHLER, S.A. Release of lysozyme from hemolymph cells of Mercenaria mercenaria during phagocytosis. Journal of Invertebrate Pathology, v.25, p.261-265, 1979. CHEON, H.M.; SHIN, S.W.; BIAN, G.; PARK, J.H.; RAIKHEL, A.S. Regulation of lipid metabolism genes, lipid carrier protein lipophorin, and its receptor during immune challenge in the mosquito Aedes aegypti. Journal of Biological Chemistry, v.281, p.8426-8435, 2006. CHINZEI, Y.; CHINO, H.; TAKAHASHI, K. Purification and properties of vitellogenin and vitellin from a tick, Ornithodoros moubata. Journal of Comparative Physiology, v.152B, p.13-21, 1983. CHOI, S.; HWANG, J.M.; KIM, S.I. A Colorimetric Microplate Assay Method for High Throughput Analysis of Lipase Activity. Journal of Biochemistry and Molecular Biology, v.36,p.417-420, 2003. CLARKSON, J.M.; CHARNLEY, A.K. New insights into mechanisms of fungal pathogenesis in insects. Trends Microbiology, v.4, n.5, p.197-203, 1996. COCIANCICH, S.; BULET, P.; HETRU, C.; HOFFMAN, J.A. The inducible antibacterial peptides of insects. Parasitology Today, v.10, p.132-138, 1994. COOP, R.L.; HOLMES, P.H. Nutrition and parasite interactions. International Journal for Parasitology, v.26, p.951-962, 1996. CRUZ, C.E.; FOGA?A, A.C.; NAKAYASU, E.S.; ANGELI, C.B.; BELMONTE, R.; ALMEIDA, I.C.; MIRANDA, A.; MIRANDA, M.T.M.; TANAKA, A.S.; BRAZ, G.R.; CRAIK, C.S.; SCHNEIDER, E.; CAFFREY, C.R.; DAFFRE, S. Characterization of proteinases from the midgut of Rhipicephalus (Boophilus) microplus involved in the generation of antimicrobial peptides. Parasites and Vectors, v.3, n.63, 2010. DA-SILVA VAZ, I.; MARTINEZ, R.H.M.; OLIVEIRA, A.; HECK, A.; LOGULLO, C.; GONZALES, J.C.; DEWES, H.; MASUDA A. Functional bovine immunoglobulins in Boophilus microplus hemolymph. Veterinary Parasitology, 62, 155-160, 1996. 95 DAVIS, B.J. Disc electrophoresis. II. Method and application to human serum proteins Annals of the New York Academy of Sciences, v.121, p.404-27, 1964. DE FARIA, M.R.; WRAIGHT, S.P. Mycoinsecticides and mycoacaricides: a comprehensive list with worldwide coverage and international classification of formulation types. Biological Control, v.43, p.237-256, 2007. DE GREGORIO, E.; SPELLMAN, P.T.; RUBIN, G.M.; LEMAITRE, B. Genome-wide analysis of the Drosophila immune response by using oligonucleotide microarrays. Proceedings of the National Academy of Sciences. USA, v.98, p.12590-12595, 2001. DENARDI, S.E., BECHARA, G.H., CAMARGO-MATHIAS, M.I. New morphological data on fat bodies of semi-engorged females of Amblyomma cajennense (Acari: Ixodidae). Micron, v.36, n.7, p.875-883, 2008. DENARDI, S.E.; BECHARA, G.H.; CAMARGO-MATHIAS, M.I. Fat body cells of Amblyomma cajennense partially engorged females (Acari: Ixodidae) and their role on vitellogenesis process. Experimental Parasitology, v.121, p.213?218, 2009. DENARDI, S.E.; BECHARA, G.H.; OLIVEIRA, P.R.; NUNES, E.T.; SAITO, K.C.; CAMARGO-MATHIAS, M.I. Morphological characterization of the ovary and vitellogenesis dynamics in the tick Amblyomma cajennense (Acari: Ixodidae). Veterinary Parasitology, v.125, p.379?395, 2004. DUMAS, C.; MATHA, V.; QUIOT, J.M.; VEY, A. Effects of destruxins, cyclic depsipeptide mycotoxins, on calcium balance and phosphorylation of intracellular proteins in lepidopteran cell lines. Comparative Biochemistry and Physiology ? Part C: Toxicology and Pharmacology, v.114, p.213?219, 1996. EGGENBERGER, L.R.; LAMORREAUX, W.J.; COONS, L.B. Hemocytic encapsulation of implants in the tick, Dermacentor variabilis. Experimental and Applied Acarology, v.9, p.279-287, 1990. ELEFTHERIANOS, I.; MAROKHAZI, J.; MILLICHAP, P.J.; HODGKINSON, A.J.; SRIBOONLERT, A.; FFRENCH-CONSTANT, R.H.; REYNOLDS, S.E. Prior infection of Manduca sexta with non-pathogenic Escherichia coli elicits immunity to pathogenic Photorhabdus luminescens: roles of immune-related proteins shown by RNA interference. Insect Biochemistry and Molecular Biology, v.36, n.6, p.517?525, 2006. ESTEVES, E.; FOGA?A, A.C.; MALDONADO, R.; SILVA, F.D; MANSO, P.P.; PELAJOMACHADO, M.; VALLE, D.; DAFFRE, S. Antimicrobial activity in the tick Rhipicephalus (Boophilus) microplus eggs: Cellular localization and temporal expression of microplusin during oogenesis and embryogenesis. Development and Comparative Immunology, v.33, n.8, p.913-919, 2009. FAHMY, A.S.; ABDEL-GANY, S.S.; MOHAMED, T.M.; MOHAMED, S.A. Esterase and lipase in camel tick Hyalomma dromedarii (Acari: Ixodidae) during embryogenesis Comparative Biochemistry and Physiology Part B, v.137, p.159?168, 2004. FARGUES, J.; GOETEL, M.S.; SMITS, N.; OUEDRAOGO, A.; VIDAL, C.; LACEY, L.A.; LOMER, C.J.; ROUGIER, M. Variability in susceptibility to simulated sunlight of conidia among isolates of entomopathogenic Hyphomycetes. Mycopathologia, v.135, p.171?181, 1996. FARGUES, J.F.; ROBERT, P.H. Effects of passing through scarabeid hosts on virulence and host specificity of two strains of the entomopathogenic hyphomycete Metarhizium anisopliae. Canadian Journal of Microbiology, v.29, p.576-583, 1983. 96 FEHLBAUM, P.; BULET, P.; MICHAUT, L.; LAGUEUX, M.; BROEKAERT, W. F.; HETRU, C.; HOFFMANNH, J.A. Insect Immunity: Septic injury of Drosophila induces the synthesis of a potent antifungal peptide with sequence homology to plant antifungal peptides. The Journal of Biological Chemistry, v.269, n.52, p.3359-63, 1994. FERNANDES, E.K.K.; BITTENCOURT, V.R.E.P. Entomopathogenic fungi against South American tick species. Experimental and Applied Acarology, v.46. p.71-93, 2008. FERNANDES, ?.K.K.; COSTA, G.L.; MORAES, A.M.L.; BITTENCOURT, V.R.E.P. Entomopathogenic potential of Metarhizium anisopliae isolated from engorged females and tested in eggs and larvae of Boophilus microplus. Journal of Basic Microbiology, v.44, p.270-274, 2004. FERNANDES, ?.K.K.; KEYSER, C.A.; CHONG, J.P.; RANGEL, D.E.N.; MILLER, M.P.; ROBERTS, D.W. Characterization of Metarhizium species and varieties based on molecular analysis, heat tolerance and cold activity. Journal of Applied Microbiology, v.108, p.115-128, 2010. FERRON, P.; FARGUES,J.; RIBA, G. Fungi as microbial insecticides against pests. In: Adora D.K., Ajello L. e Mujerjii K.G. Handbook of Applied Mycology. New York: Marcel Dekker. 1991. FOGA?A, A.C.; ALMEIDA, I.C.; EBERLIN, M.N.; TANAKA, A.S.; BULET, P.; DAFFRE. Ixodidin, a novel antimicrobial peptide from the hemocytes of the catle tick Boophilus microplus with inhibitory activity against serine proteinases. Peptides, v.27, p.667-674, 2006. FOGA?A, A.C.; LORENZINI, D.M.; KAKU, L.M.; ESTEVES, E.; BULET, P.; DAFFRE, S. Cysteine-rich antimicrobial peptides of the cattle tick Boophilus microplus: isolation, structural characterization and tissue expression profile. Developmental and Comparative Immunology, v.28, p.191?200, 2004. FOGA?A, A.C.; SILVA JR, P.I.; MIRANDA, M.T.M.; BIANCHI, A.G.; MIRANDA, A.; RIBOLLA, P.E.M.; DAFFRE, S. Antimicrobial Activity of a Bovine Hemoglobin Fragment in the Tick Boophilus microplus. The Journal of Biological Chemistry, v.274, n.36, 1999. FRAZZON, A.P.G.; JUNIOR, I.S.V.; MASUDA, A.; SCHRANK, A.; VAINSTEIN, M.H. In vitro assessment of Metarhizium anisopliae isolates to control the cattle tick Boophilus microplus. Veterinary Parasitology, v.94, p.117-125, 2000. FURLONG, J. Controle do carrapato dos bovinos na regi?o Sudeste do Brasil. Caderno T?cnico da Escola Veterin?ria UFMG, n.8, p.49-61, 1993. FURLONG, J.; MARTINS, J.R.S.; PRATA, M.C.A. Controle estrat?gico do carrapato dos bovinos. A Hora Veterin?ria, ano 23, n.137, 2004. G?DE, G.; AUERSWALD, L.; MARCO, H.G. Flight fuel and neuropeptidergic control of fuel mobilisation in the twig wilter, Holopterna alata (Hemiptera, Coreidae). Journal of Insect Physiology, v.52, p.1171-81, 2006. GAMS, W.; ROZSYPAL,J. Metarhizium flavoviride n. sp. isolated from insects and soil. Acta Botanica Neerlandica, v.2, p.518-521, 1973. GILBERT, L.I.; CHINO, H. Transport of lipids in insects. Journal of Lipid Research, v.15, p.439-456, 1974. GILLESPIE, J.P.; KANOST, M.R.; TRENCZEK, T. Biological mediators of insect immunity. Annual Review of Entomology, v.42, p.611-643, 1997. 97 GINDIN, G.; SAMISH, M.; ALEKSEEV, E.; GLAZER, I. The Susceptibility of Boophilus annulatus (Ixodidae) Ticks to Entomopathogenic Fungi. Biocontrol Science and Technology, v.11, p.111-118, 2001. GOBERT, V.; GOTTAR, M.; MATSKEVICH, A.A.; RUTSCHMANN, S.; ROYET, J.; BELVIN, M.; HOFFMANN, J.A.; FERRANDON, D. Dual activation of the Drosophila Toll pathway by two pattern recognition receptors. Science, v.302, p.2126-2130, 2003. GOLODNE, D.M.; MONTEIRO, R.Q.; GRA?A-SOUZA, A.V.; SILVA-NETO, M.A.C.; ATELLA, G.C. Lysophosphatidylcholine acts as na anti-hemostatic molecule in the saliva of the blood-sucking bug Rhodnius prolixus. Journal of Biological Chemistry, v.278, n.30, p.2766-2771, 2003. GONDIM, K.C.; OLIVEIRA, P.L.; COELHO, H.S.L.; MASUDA, H. Lipophorin from Rhodnius prolixus: purification and partial characterization. Insect Biochemistry, v.19, p.153-161, 1989. GONZALES, J. C. O controle do carrapato dos bovinos. Porto Alegre: Sulina, 1975. 103p. GONZALES, J.C. O carrapato do boi: vida, resist?ncia e controle. S?o Paulo: Mestre Jou, 1974. 101p. GONZ?LES, J.C. O carrapato dos bovinos Boophilus microplus (Can. 1887) (Revis?o hist?rica e conceitual). A Hora Veterin?ria, ano 21, n.125, p.23-28, 2002 GORMAN, M.J.; PASKEWITZ, S.M. Serine proteases as mediators of mosquito immune responses. Insect Biochemistry and Molecular Biology, v.31, p.257?62, 2001. GOTTAR, M.; GOBERT, V.; MATSKEVICH, A.; REICHHART, J.M.,; WANG, C.; BUTT, T.M.; BELVIN, M.; HOFFMANN, J.A.; FERRANDON, D. Dual detection of fungal infections in Drosophila by recognition of glucans and sensing of virulence factors. Cell, v.127, p.1425-1437, 2006. GRISI, L.; MASSARD, C.L.; BORJA, G.E.M.; PEREIRA, J.B. Impacto econ?mico das principais ectoparasitoses em bovinos no Brasil. A Hora Veterin?ria, ano 21, n.125, p.23-28, 2002. GRUBHOFFER, L.; VERES, J.; DUSBABEK, F. Lectins as the molecular factors of recognition and defense reactions of ticks. In: Dusbabek, F., Bukva, V. (Eds.), Modern Acarology, 2. Academia, Prag, p.381-388, 1991. GUDDERRA, N.P.; NEESE, P.A.; SONENSHINE, D.E.; APPERSON, C.S.; ROE, R.M. Developmental profile, isolation, and biochemical characterization of a novel lipoglycohemecarrier protein from the American dog tick, Dermacentor variabilis (Acari:Ixodidae) and observations on a similar protein in the soft tick, Ornithodoros parkeri (Acari:Argasidae). Insect Biochemistry and Molecular Biology, v.31, p.299-311, 2001. GUDDERRA, N.P.; SONENSHINE, D.E.; APPERSON, C.S.; ROE, R.M. Hemolymph proteins in ticks. Journal of Insect Physiology, v.48, p.269-278, 2002a. GUDDERRA, N.P.; SONENSHINE, D.E.; APPERSON, C.S.; ROE, R.M. Tissue distribuition and characterization of predominant hemolymph carrier proteins from Dermacentor variabilis and Ornithodoros parkeri. Journal of Insect Physiology, v.48, p.19- 29, 2002b. GUIMAR?ES, A.M.; LIMA, J.D.; RIBEIRO, M.F.B. Sporogony and experimental transmission of Babesia equi by Boophilus microplus. Parasitology Research, v.84, p.323- 327, 1998 98 HANCOCK, R.E.W.; BROWN, K.L.; MOOKHERJEE, N. Host defence peptides from invertebrates ? emerging antimicrobial strategies. Immunobiology, v.211, p.315-322, 2006. HANCOCK, R.E.W.; LEHRER, R. Cationic peptides: a new source of antibiotics. Trends in Biotechnology, v.16, p.82?88, 1998. HANCOCK, R.E.W.; SCOTT, M.G. The role of antimicrobial peptides in animal defenses. Proceedings of the National Academy of Sciences, v.97, p.8856-8861, 2000. HAWKSWORTH, D.L. Micologist?s handbook. 2?ed. England, Kew Surrey: CAB Press, 1977. 231p HAWKSWORTH, D.L.; KIRK, P.M.; SUTTON, B.C.; PEGLER, D.N. ( Ainsworth and Bisby?s) Dictionary of the Fungi, 8?ed. , CAB International, Wallingford, UK, 616p., 1995. HETRU, C.; HOFFMAN, D.; BULET, P. Antimicrobial peptides from insects. In: Brey, P.T., Hultmark, D. (Eds.), Molecular mechanisms of immune responses in insects. Chapman and Hall, New York, 1998. HEUCHERT, C.M.; DE, G.V.JR.; DE ATHAIDE, D.F.; BOSE, R.; FRIEDHOFF, K.T. Seroepidemiologic studies on Babesia equi and Babesia caballi infections in Brazil. Veterinary Parasitology, v.85, p.1-11, 1999. HOFFMANN, J.A. The immune response of Drosophila. Nature, v.426, p.33-38, 2003. HOFFMANN, J.A.; KATATOS, F.C.; JANEWAY, C.A.JR.; EZEKOWITZ, R.A.B. Phylogenetic perspectives in innate immunity. Science, v.284, p.1313-1318, 1999. HOFFMANN, J.A.; REICHHART, J.M. Drosophila innate immunity: an evolutionary perspective. Nature Immunology, v.3, n.2, 2002. HORN, S.C.; ARTECHE, C.C.P. Situa??o parasit?ria da pecu?ria no Brasil. A Hora Veterin?ria, v.4, p.12-32, 1985. HORWITZ, J.; PERLMAN, R.L. Measurement of inositol phospholipid-metabolism in PC12 pheochromocytoma cells. Methods in Enzymology, v.141, p.169-175, 1987. HU, Q.B.; REN, S.X.; WU, J.H.; CHANG, J.M.; MUSA, P.D. Investigation of destruxin A and B from 80 Metarhizium strains in China, and the optimization of cultural conditions for the strain MaQ10. Toxicon, v.48, p.491-498, 2006. HUNT, D.W.A. Absence of fatty acid germination inhibitors for conidia of Beauveria bassiana on the integument of the bark beetle Dendroctonus ponderosae (Coleoptera: Scolytidae). The Canadian Entomologist, v.118, p.837-838, 1986. IWANAGA S. The molecular basis of innate immunity in the horseshoe crab. Current Opinion in Immunology, v.14, p.87?95, 2002. IWANAGA, S.; LEE, B.L. Recent advances in the innate immunity of invertebrates animals. Insect Biochemistry and Molecular Biology, v.38, p.128-150, 2005. JAEGER, K.E.; EGGERT, T. Lipases for biotechnology. Current Opinion in Biotechnology, v.13, p.390?397, 2002. JAMES, A.M.; OLIVER, J.H. Vitellogenesis and its hormonal regulation in Ixodida: preliminary results. In: Needhan, G.R., Mitchell, R., Horn, D.J., Welbourn, W.C. (Eds), Acarology IX, v.2. Ohio Biological Survey, Columbus, Ohio, 1999. JIANG, H.; KANOST, M.R. The clip-domain family of serine proteinases in arthropods. Insect Biochemistry and Molecular Biology, v.30, p.95-105, 2000. 99 JOHNS, R.; OHNISHI, J.; BROADWATER, A.; SONENSHINE, D.E.; DE SILVA, A.M.; HYNES, W.L. Contrasts in tick innate immune responses to Borrelia burgdorferi challenge: immunotolerance in Ixodes scapularis versus immunocompetence in Dermacentor variabilis (Acari: Ixodidae). Journal of Medical Entomology 38:99?107, 2001a. JOHNS, R.; SONENSHINE, D. E.; HYNES, W. L Control of bacterial infections in the hard tick Dermacentor variabilis (Acari: Ixodidae): evidence for the existence of antimicrobial proteins in tick hemolymph. Journal of Medical Entomology, v. 35, n.4, p. 458-464, 1998. JOHNS, R.; SONENSHINE, D.E.; HYNES, W.L. Identification of a defensin from the hemolymph of the American dog tick, Dermacentor variabilis. Insect Biochemistry and Moecular Biology, v.31, p.857?865, 2001b. JURETIC, D.; VUKICEVIC, D.; ILIC, N.; ANTCHEVA, N.; TOSSI, A. Computational design of highly selective antimicrobial peptides. Journal of Chemical Information and Modeling, v.49, p.2873?2882, 2009. KABARA, J.J.; SWIECZKOWSKI, D.M.; CONLEY, A.J.; TRUANT, J.P. Fatty acids and derivatives as antimicrobial agents. Antimicrobial Agents and Chemotherapy, v.2, n.1, p.23-28, 1972. KADOTA, K.; SATOH, E.; OCHIAI, M.; INOUE, N.; TSUJI, N.; IGARASHI, I.; NAGASAWA, N.; MIKAMI, T.; CLAVERIA, F.G.; FUJISAKI, K. Existence of phenol oxidase in the argasid tick Ornithodoros moubata. Parasitology Research, v.88, p.78-784, 2002. KANOST, M.R. Serine proteinase inhibitors in arthropod immunity. Developmental and Comparative Immunology, v.23, p.291-301, 1999. KANOST, M.R.; JIANG, H.; Yu, X.Q. Innate immune responses of a lepidopteran insect, Manduca sexta. Immunological Reviews, v.198, p.97?105, 2004. KAUFMAN, W.R.; PHILLIPS, J.E. Ion and water balance in the ixodid tick, Dermacetor andersoni. I. Routes of ion and water excretion. Journal of Experimental Biology, 58, p.523-536, 1973. KAWOOYA, J. K.; LAW, J.H. Role of lipophorin in lipid transport to insect egg. The Journal of Biological Chemistry, v.263, p.8748-8753, 1988. KEELEY, L.L. Comprehensive Insect Physiology, Biochemistry and Pharmacology, Physiology and biochemistry of the fat body. In: Kerkut, G.A. and Gilbert, L.I. (Eds) 1985 v.3. Integument, Respiration and Circulation. Pergamon Press, Oxford. 1985. KLAUDINY, J.; ALBERT, S.; BACHANOVA, K.; KOPERNICKY, J.; SIMUTH, J. Two structurally different defensin genes, one of them encoding a novel defensin isoform, are expressed in honeybee Apis mellifera. Insect Biochemistry and Molecular Biology, v.35, p.11-22, 2005. KONGSUWAN, K.; JOSH, P.; ZHU, Y.; PEARSON, R.; GOUGH, J.; COLGRAVE, M.L. Exploring the midgut proteome of partially fed female cattle tick (Rhipicephalus (Boophilus) microplus). Journal of Insect Physiology, v.56, p.212-226, 2010. KOPACEK, P.; WEISE, C.; SARAVANAN, T.; VITOVA, K.; GRUBHOFFER, L. Characterization of an -macroglobulin-like glycoprotein isolated from the plasma of the soft tick Ornithodoros moubata. European Journal of Biochemistry, v.267, p.465-475, 2000. 100 KOVAR, V.; KOPACEK, P.; GRUBHOFFER, L. Characterization of an -macroglobulinlike glycoprotein isolated from the plasma of the soft tick Ornithodoros moubata. European Journal of Biochemistry, v.267, p.465-475, 2000. KUHN, K.H.; HAUG, T. Ultrastructure, cytochemical and immunocytochemical characterization of hemocytes of the hard tich Ixodes ricinus (Acari: Chelicerata). Cell and Tissue Research, v.277, p.493-504, 1994. KUROOKA, S.; OKAMOTO, S.; HASHIMOTO, M. A novel and simple colorimetric assay for human serum lipase. The Journal of Biochemistry, v.81, p.361-369, 1977. LAEMMLI, U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, v.227, p.680?685, 1970. LAMBERTY, M.; ZACHARY, D.; LANOT, R.; BORDEREAU, C.; ROBERT, A.; HOFFMANN, J.A.; BULET, P. Constitutive expression of a cysteine-rich antifungal and a linear antibacterial peptide in a termite insect. Journal Biological Chemistry, v.276, p.4085- 4092, 2001. LAMPROU, I.; MAMALI, I.; DALLAS, K.; FERTAKIS, V.; LAMPROPOULOU, M.; MARMARAS, V.J. Distinct signalling pathways promote phagocytosis of bacteria, latex beads and lipopolysaccharide in medfly haemocytes. Immunology, v.121, n.3, p.314-327, 2007. LAVINE, M.D.; STRAND, M.R. Haemocytes from Pseudomonas includens express multiple and integrin subunits. Insect Molecular Biology, v.12, n.5, p.441-452, 2003. LEE, H.; KWON, H.M.; PARK, J.W.; KUROKAWA, K.; LEE, B.L. N-terminal GNBP homology domain of Gram-negative binding protein 3 functions as a beta-1,3-glucan binding motif in Tenebrio molitor. Biochemistry and Molecular Biology Reports, v.42, p.506-510, 2009. LEMAITRE, B. The road to Toll. Nature Reviews Immunology, v.4, p.521?527, 2004. LEMAITRE, B.; HOFFMANN, J. The host defense of Drosophila melanogaster Annual Review of Immunology, v.25, p.697?743, 2007. LEMAITRE, B.; REICHHART, J.; HOFFMANN, J. Drosophila host defense: differential induction of antimicrobial peptide genes after infection by various classes of microorganisms. Proceedings of the National Academy of Sciences. USA, v.94, p.14614-14619, 1997. LEVASHINA, E.A. Innate immune response of Anopheles gambiae. Insect Biochemistry and Molecular Biology, v.34, p.673-678, 2004. LEVASHINA, E.A.; LANGLEY, E.; GREEN, C.; GUBB, D.; ASHBURNER, M.; HOFFMANN, J.A.; REICHHART, J.M. Constitutive activation of toll mediated antifungal defense in serpin-deficient Drosophila. Science, v.285, p.1917-1919, 1999. LEVY, F.; BULET, P.; EHRET-SABATIER, L. Proteomic analysis of the systemic immune response of Drosophila. Molecular and Cellular Proteomics, v.3, p.156-166, 2004. LIMA, C.A.; TORQUATO, R.J.S.; SASAKI, S.D.; JUSTO, G.Z.; TANAKA, A.S. Biochemical characterization of a Kunitz type inhibitor similar to dendrotoxins produced by Rhipicephalus (Boophilus) microplus (Acari: Ixodidae) hemocytes. Veterinary Parasitology, v.167, p.279-287, 2010. LOHNER, K. The role of membrane lipid composition in cell targeting of antimicrobial peptides. Development of novel antimicrobial agents: emerging strategies. Lohner, K. (ed), Horizon Scientific Press, England, p.149-165, 2001. 101 LOMER, C.J.; BATEMAN, R.P.; JOHNSON, D.L.; LANGEWALD, J.; THOMAS, M. Biological control of locusts and grasshoppers. Annual Review of Entomology, v.46, p.667- 702, 2001. LOPES, R.B.; ALVES, S.B.; PADULLA, L.F.L.; P?REZ, C.A. Efici?ncia de formula??es de Beauveria bassiana and Metarhizium anisopliae para o controle de ninfas de Amblyomma cajennense (FABRICIUS, 1787). Revista Brasileira de Parasitologia Veterin?ria, v.16, p.27?31, 2007. LOWENBERGER, C.; BULET, P.; CHARLET, M.; HETRU, C.; HODGEMAN, B.; CHRISTENSEN, B.M.; HOFFMAN, J.A. Insect immunity: isolation of three novel inducible antimicrobial defensins from the vector mosquito, Aedes aegypt. Insect Biochemistry and Molecular Biology, v.25, p.867-873, 1995. LU, Y.; LU, F.; WANG, X.; BIE, X.; SUN, H.; WUYUNDALAI,; LU, Z. Identification of bacteria producing a thermophilic lipase with positional non-specificity and characterization of the lipase. Annals of Microbiology, v.59, n.3, p.565-571, 2009. LUBECK, I.; ARRUDA, W.; SOUZA, B.K.; STANIS?UASKI, F.; CARLINI, C.R.; SCHRANK, A.; VAINSTEIN, M.H. Evaluation of Metarhizium anisopliae strains as potential biocontrol agents of the tick Rhipicephalus (Boophilus) microplus and the cotton stainer Dysdercus peruvianus. Fungal Ecology, v.1, p.78-88, 2008. LUO, T.; ZHANG, X.; SHAO, Z.; XU, X. PmAV, a novel gene involved in virus resistance of shrimp Penaeus monodon. FEBS Letters, v.551, p.53-57, 2003. LUZ, C.; TIGANO, M.S.; SILVA, I.G.; CORDEIRO, C.M.T.; ALJANABI, S.M. Selection of Beauveria bassiana and Metarhizium anisopliae isolates to control Triatoma infestans. Mem?rias do Instituto Oswaldo Cruz, v.93, n.6, p.839-846, 1998. MANZANO-ROMAN, R.; ALMAZ?N, C.; NARANJO, V.; BLOUIN, E.F.; KOCAN, K.M.; DE LA FUENTE, J. Expression of perilipin in human promyelocytic cells in response to Anaplasma phagocytophilum infection results in modified lipid metabolism. Journal of Medical Microbiology, v.57,p.159-163, 2008. MARKWELL, M.A.K.; HAAS, S.M.; BIEBER, L.L.; TOLBERT, N.E. A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samples. Analitical Biochemistry, v.87, p.206?210, 1978. MARMARAS. V.J.; LAMPROPOULOU, M. Regulators and signalling in insect haemocyte immunity. Cellular Signalling, v.21, p.186-195, 2009. MARTIN, H.S.; TIMOTHY, S.; MANICKAM, S.; KENNETH, S.; JOHN, H.L. Proenzyme of Manduca sexta phenoloxidase: purification, activation, substrate specificity of the active enzyme, and molecular cloning. Proceeding of the National Academy of Science, USA, v.92, p.7764-7768, 1995. MAYA-MONTEIRO, C.M.; DAFFRE, S.; LOGULLO, C.; LARA, F.A.; ALVES, E.W.; CAPURRO, M.L.; ZINGALI, R.; ALMEIDA, I.C.; OLIVEIRA, P.L. HeLp, a Heme Lipoprotein from the hemolymph of the catlle tick, Boophilus microplus. The Journal of Biological Chemistry, v.275, n.47, p.36584-36589, 2000. MEDZHITOV, R.; JANEWAY, J.C.A. Decoding the patterns of self and nonself by the innate immune system. Science, v.296, p.298-300, 2002. METCHNIKOFF, E. Diseases of the larva of the grain weevil. Insects harmful to agriculture. Odessa, UA: Odessa Zemstvo Office (in Russian), 1879. 102 MICHEL, K.; KAFATOS, F.C. Mosquito immunity against Plasmodium. Insect Biochemistry and Molecular Biology, v.35, p.677?689, 2005. MICHEL, T.; REICHHART, J.M.; HOFFMANN, J.A.; ROYET, J. Drosophila Toll is activated by Gram-positive bacteria through a circulating peptidoglycan recognition protein. Nature, v.414, p.756-759, 2001. MONTEIRO, S.G.; BAHIENSE, T.C.; BITTENCOURT, V.R.E.P. A??o do fungo Beauveria bassiana (Balsamo) Vuillemin, 1912 sobre a fase parasit?ria do carrapato Anocentor nitens (Neumann, 1897) Schulze, 1937 (Acari: Ixodidae). Ci?ncia Rural, Santa Maria, v.33, p.559- 563, 2003. MOOKHERJEE, N.; HANCOCK, R.E. Cationic host defence peptides: innate immune regulatory peptides as a novel approach for treating infections. Cellular and Molecular Life Sciences, v.64, n.7-8, p.922-933, 2007. MORET, Y.; SCHMIDT-HEMPEL, P. Survival for immunity: the price of immune system activation for bumblebee workers. Science, v.290, p.1166-1198, 2000. MULLER, E.E.; FRIED, D.; SHERMA, J. HPTLC analysis of neutral lipids in Biomphalaria glabrata snails infected with Schistosoma mansoni (Trematoda). Journal of Planar Chromatography - Modern TLC, v.13, p.228?231, 2000. MULLER, U.; VOGEL, P.; ALBER, G.; SCHAUB, G. The Innate Immune System of Mammals and Insects. Egesten A, Schmidt A, Herwald H (eds): Trends in Innate Immunity. Contrib Microbiol. Basel, Karger, v.15, p.21-44, 2008. NAKAJIMA, Y.; VAN DER GOES VAN NATERS-YASUI, A.; TAYLOR, D.; YAMAKAWA, M. Two isoforms of a member of the arthropod defensin family from the soft tick, Ornithodoros moubata (Acari:Argasidae). Insect Biochemistry and Molecular Biology, v.31, p.747?751, 2001. NAKAJIMA, Y.; VAN DER GOES VAN NATERS-YASUI, A.; TAYLOR, D.; YAMAKAWA, M. Antibacterial peptide defensin is involved in midgut immunity of the soft tick, Ornithodoros moubata. Insect Molecular Biology, v.11, p.611?618, 2002. NAPPI, A.J.; OTTAVIANI, E. Cytoxicity and cytotoxic molecules in invertebrates. Bioessays, v.22, p. 469?480, 2000. NIRMALA, X.; KODRIK D.; ZUROVEC, M.; SEHNAL, F. Insect silk contains both a Kunitz-type and a unique Kazal-type proteinase inhibitor. European Journal of Biochemistry, v.268, p.2064-2073, 2001. O?HAGAN, J.E. Boophilus microplus: Digestion of hemoglobins by the engorged females tick. Experimental Parasitology, v.35, p.110-118, 1974. OBENCHAIN, F.D.; OLIVER, J.H., Jr. A qualitative analysis of the form, function and interrelationships of the fat body and associated tissues in adult ticks (Acari: Ixodoidea). Journal of Experimental Biology, v.186, p.217-236, 1973. PAL, S.; ST LEGER, R.J.; WU, L.P. Fungal peptide destruxin A plays a specific role in suppressing the innate immune response in Drosophila melanogaster. Journal of Biological Chemistry, v. 282, p.8969-8977, 2007. PARK, B.H. Inactive but dimeric form of lipoprotein lipase in human plasma. J. Biochemistry and Molecular Biology, v.34, p.329-333, 2001. PENNA, V.M. Boophilus microplus: A resist?ncia gen?tica do hospedeiro como forma de controle. Cadernos T?cnicos da Escola de Veterin?ria da UFMG, v.4, p.3-65, 1990. 103 PEREIRA, L.S.; OLIVEIRA, P.L.; BARJA-FIDALGO, C.; DAFFRE, S. Production of reactive oxygen species by hemocytes from the cattle tick Boophilus microplus. Experimental Parasitology, v.99, p.66-72, 2001. PLASCENCIA, Y.G.; ALBORES, V.F.; CIAPARA, H.I. Penaeid shrimp hemolymph lipoproteins. Aquaculture, v. 191, p.177-189, 2000. RACHINSKY, A.; GUERRERO, F.D.; SCOLES, G.A. Proteomic profiling of Rhipicephalus (Boophilus) microplus midgut responses to infection with Babesia bovis. Veterinary Parasitology, v.152, p.294-313, 2008. RANGEL, D.E.N.; BRAGA, G.U.L.; FLINT, S.D.; ANDERSON, A.J.; ROBERTS, D.W. Variations in UV-B tolerance and germination speed of Metarhizium anisopliae conidia produced on insects and artificial substrates. Journal of Invertebrate Pathology, v. 87, p.77?83, 2004. RAVICHANDRAN, S.; WAHIDULLA, S.; D?SOUZA, L.; RAMESHKUMAR, G. Antimicrobial lipids from the hemolymph of Brachyuran crabs. Applied Biochemistry and Biotechnology, v.162, n.4, p.1039-1051, 2009. REIS, P.; HOLMBERG, K.; WATZKE, H.; LESER, M.E.; MILLER, R. Lipases at interfaces: A review. Advances in Colloid and Interface Science, v.147-148, p.237?250, 2009. RIVALIER, E. & SEYDEL, S. Nouveau procede de culture sur lames g?los?s appliqu? a l??tude microscopique de champignos deteignes. Annals of Parasitology, v.10, n.5, p.444- 452, 1932. RODRIGUES, J.; BRAYNER, F.A.; ALVES, L.C; DIXIT, R.; BARILLAS-MURY, C. Hemocyte differentiation mediates innate immune memory in Anopheles gambiae mosquitoes. Science, v.329, p.1353-1355, 2010. ROE, R.M.; DONOHUE, K.V.; KHALIL, S.M.; SONENSHINE, D.E. Hormonal regulation of metamorphosis and reproduction in ticks. Frontiers in Bioscience, v.1, n.13, p.7250-7268, 2008. ROHLFS, M.; CHURCHILL, A.C. Fungal secondary metabolites as modulators of interactions with insects and other arthropods. Fungal Genetics and Biology, v.48, n.1, p.23- 34, 2011. ROMBACH, M.C.; HUMBER, R.A.; EVANS, H.C. Metarhizium album a fungal pathogen of leaf- and plant hoppers of rice. Transactions of the British Mycological Society, v.37, p.37- 45, 1987. ROSELL, R.; COONS, L.B. Determination of vitellogenin titer in the hemolymph of Dermacentor variabilis (Acarina: Ixodidae) using an indirect enzyme-linked immunosorbent assay. Journal of Medical Entomology, v.28, p.41-45, 1991. ROTH, O.; JOOP, G.; EGGERT, H.; HILBERT, J.; DANIEL, J.; SCHMID-HEMPEL, P.; KURTZ, J. Paternally derived immune priming for offspring in the red flour beetle, Tribolium castaneum. Journal of Animal Ecology, v.79, p.403?413, 2010. ROXSTROM-LINDQUIST, K.; TERENIUS, O.; FAYE, I. Parasite-specific immune response in adult Drosophila melanogaster: a genomic study. European Molecular Biology Organization, v.5, n.2, 2004. RUDENKO, N.; GOLOVCHENKO, M.; GRUBHOVER, L. Gene organization of a novel defensin of Ixodes ricinus: first annotation of an intron/exon structure in a hard tick defensin 104 gene and first evidence of the occurrence of two isoforms of one member of the arthropod defensin family. Insect Molecular Biology, v.16, p.501?507, 2007. RUIZ, J.I.; OCHOA, B. Quantification in the subnanomolar range of phospholipids and neutral lipids by monodimensional thin-layer chromatography and image analysis. Journal of Lipid Research, v. 38, p.1482?1489, 1997. RUSSO, J.; BREH?LIN, M.; CARTON, Y.; Haemocyte changes in reistant and susceptible strains of D. melanogaster caused by virulent and avirulent strains of the parasitic wasp Leptopilina boulardi. Journal of Insect Physiology, v.47, p.167-172, 2001. RYAN, R.O., VAN DER HORST, D.J. Lipid transport biochemistry and its role in enzyme production. Annual Review of Entomology, v.45, p.233?260, 2000. SAMISH, M.; REHACEK, J. Pathogens and predators of ticks and their potential in biological control. Annual Review of Entomology, v.44, p. 159-182, 1999 SANDS, J. A.; AUPERIN, D. D.; LANDIN, P.D.; REINHARDT, A.; CADDEN, S. P. Antiviral effects of fatty acids and derivatives: lipid-containing bacteriophages as a model system. In J. J. Kabara (Ed.), Symposium on the pharmacological effect of lipid. The American Oil Chemists Society: ChPAMaign, IL, USA, 1978. SAXENA, R.K.; SHEORAN, A.; GIRI, B.; DAVIDSON, W.S. Purification strategies for microbial lipases. Journal of Microbiological Methods, v52, p.1?18, 2003. SCHERFER, C.; KARLSSON, C.; LOSEVA, O.; BIDLA, G.; GOTO, A.; HAVEMANN, J.; DUSHAY, M.S.; THEOPOLD, U. Isolation and characterization of hemolymph clotting factors in Drosophila melanogaster by a pullout method. Current Biology, v.14, p.625-629, 2004. SCHMID-HEMPEL, P. Evolutionary ecology of insect immune defenses. Annual Review of Entomology, v.50, p.529-551, 2005. SCHRANK, A.; VAINSTEIN, M.H. Metarhizium anisopliae enzymes and toxins Toxicon, v.56, p.1267-1274, 2010. SCHRIEFER, M.E. Vitellogenesis in Hyalomma dromedarii (Acari:Ixodidae): a model for analysis of endocrine regulation in ixodid ticks. PhD. Dissertation. Old Dominion University, Norfolk, Virginia, USA, 1991. SCHUHMANN, B.; SEITZ, V.; VILCINSKAS, A.; PODSIADLOWSKI, L. Cloning and expression of Gallerimycin, an antifungal peptide expressed in immune response of greater wax moth larvae, Galleria mellonella. Archives of Insect Biochemistry and Physiology, v.53, p.125-133, 2003. SEVALA, V.; SHU, S.; RAMASWAMY, S.B.; SCHAL, C. Lipophorin of female Blatella germanica (L.): characterization and relation to hemolymph titers of juvenile hormone and hydrocarbons. Journal of Insect Physiology, v. 45, p.431-441, 1999. SHAI, Y. Mechanism of the binding, insertion and destabilization of phospholipid bilayer membranes by alpha-helical antimicrobial and cell non-selective membrane-lytic peptides. Biochimica et Biophysica Acta, v.1462, p.55?70, 1999. SILVA JR, P.I.; DAFFRE, S.; BULET, P. Isolation and Characterization of Gomesin, an 18- Residue Cysteine-rich Defense Peptide from the Spider Acanthoscurria gomesiana Hemocytes with Sequence Similarities to Horseshoe Crab Antimicrobial Peptides of the Tachyplesin Family . The Journal of Biological Chemistry, v.275, n.43, p.33464?3347, 2000. 105 SILVA, C.; GARY, B.D.; RAU, M.E. Interaction of hemocytes and prophenoloxidase system of fifth instar nymphs of Acheta domesticus with bacteria. Developmental and Comparative Immunology, v.24, p.367-379, 2000. SILVA, S.B.; BITTENCOURT, V.R.E.P. Avalia??o da resposta celular de f?meas ingurgitadas de Boophilus microplus (CANESTRINI, 1887) inoculadas com Metarhizium anisopliae, Beauveria bassiana, Penicillium corylophilum ou Fusarium oxysporum. Revista Brasileira de Parasitologia Veterin?ria, v.15, p.151-156, 2006a. SILVA, S.B.; SAVASTANO, G.; BITTENCOURT, V.R.E.P. Tipos celulares envolvidos na resposta imune de f?meas ingurgitadas de Boophilus mcroplus inoculados com Metarhizium anisopliae e Penicillium sp. Revista Brasileira de Parasitologia Veterin?ria, v.15, n.3, p.129-131, 2006b. SILVA, W.O.B.; MITIDIERI, S.; SCHRANK, A.; VAINSTEIN, M.H. Production and extraction of an extracellular lipase from the entomopathogenic fungus Metarhizium anisopliae. Process Biochemistry, v.40, n.1, p.321-326, 2005. SILVA, W.O.B.; SANTI, L.; BERGER, M.; PINTO, A.F.M.; GUIMAR?ES, J.A.; SCHRANK, A.; VAINSTEIN, M.H. Characterization of a spore surface lipase from the biocontrol agent Metarhizium anisopliae. Process Biochemistry, v.44, p.829?834, 2009. SONENSHINE, D.E. Biology of ticks 1. New York, Oxford University Press, 1991. 447p. SONENSHINE, D.E.; HYNES, W.L. Molecular characterization and related aspects of the innate immune response in ticks. Frontiers in Bioscience, v.13, p.7046?7063, 2008. SONENSHINE, D.E.; HYNES, W.L.; CERAUL, S.M.; MITCHELL, R.; BENZINE, T. Host blood proteins and peptides in the midgut of the tick Dermacentor variabilis contribute to bacterial control. Experimental and Applied Acarology, v36, p.207-223, 2005. SOROKIN, N. Plant parasites of man and animals as causes of infectious diseases. Journal of Military Medical, v.2, p.268-291, 1883. SREE, K.S.; PADMAJA, V.; MURTHY, Y.L. Insecticidal activity of destruxin, a mycotoxin from Metarhizium anisopliae (Hypocreales), against Spodoptera litura (Lepidoptera: Noctuidae) larval stages. Pest Management Science, v.64, p.119-125, 2008. ST. LEGER, R.J.; MAY, B.; ALLEE, L.L.; FRANK, D.C.; STAPLES, R.C.; ROBERTS, D.W. Genetic differences in allozymes and in formation of infection structures among isolates of the entomopathogenic fungus Metarhizium anisopliae. Journal of Invertebrate Pathology, v.60, p.89-101, 1992. STANLEY, D.; MILLER, J.; TUNAZ, H. Eicosanoid Actions in Insect Immunity. Journal of Innate Immunity, v.1, n.4, 2009. STAUBLI, F.; JORGENSEN, T.J.; CAZZAMALI, G.; WILLIAMSON, M.; LENZ, C.; SONDERGAARD, L.; ROEPSTORFF, P.; GRIMMELIKHUIJZEN, C.J. Molecular identification of the insect adipokinetic hormone receptors. Proceedings of the National Academy of Sciences U.S.A., v.99, p.3446-3451, 2002. STEELE, J. H. Control of metabolic processes. In: Kerkut, G.A.; Gilbert, L.I., Editors, 1985. Comprehensive Insect Biochemistry, Physiology and Pharmacology, Pergamon, Oxford, p. 99-146, 1985. STEHR, F.; FELK, A.; G?CSER, A.; KRETSCHMAR, M.; MAHNB, B.; NEUBER, K., HUBE, B.; SCH?FER, W. Expression analysis of the Candida albicans lipase gene family 106 during experimental infections and in patient sPAMles. FEMS Yeast Research, v.4, p.401? 408, 2004. STOPFORTH, E.; NEITZ, A.W.H.; GASPAR, A.R.M. A proteomics approach for the analysis of hemolymph proteins involved in the immediate defense response of the soft tick, Ornithodoros savignyi, when challenged with Candida albicans. Experimental and Applied Acarology, v.51, p.309-325, 2010. STRAND, M.R.; PECH, L.L. Immunological basis for compatibility in parasitoid-host relationships. Annual Review Entomology, v.40, p.31-56, 1995. STUART, J.A., GILLIS, T.E.; BALLANTYNE, J.S. Compositional correlates of metabolic depression in the mitochondrial membranes of estivating snails. American Journal of Physiology -Regulatory, Integrative and Comparative Physiology, v.44, n.6, p.1977-1982, 1998. SUN, J.; HIRAOKA, T.; DITTMER, N.T.; CHO, K.; RAIKHEL, A.S. Lipophorin as a yolk precursor in the mosquito, Aedes aegypti. Insect Biochemistry and Molecular Biology, v. 30, p. 1161-1171, 2000. TANG, H.; KAMBRIS, Z.; LEMAITRE, B.; HASHIMOTO, C.Two proteases defining a melanization cascade in the immune system of Drosophila. Journal of Biological Chemistry, v.281, p.28097-28104, 2006. TAYLOR, D. Innate immunity in ticks: a review. Journal of the Acarological Society of Japan v.15, p.109?127, 2006. THOMAS, M.B.; READ, A.F. Fungal bioinsecticide with a sting. Nature Biotechnology, v.25, p.1367-1368, 2007. THOMPSON, D.M.; KHALIL, S.M.S.; JEFFERS, L.A.; SONENSHINE, D.E.; MITCHELL, R.D.; OSGOOD, C.J.; ROE, R.M. Sequence and the developmental and tissue-specific regulation of the first complete vitellogenin messenger RNA from ticks responsible for heme sequestration. Insect Biochemistry and Molecular Biology, v.37, p.363-374, 2007. THOMPSON, S.N. Effect of Schistosoma mansoni on the gross lipids composition of its vector Biomphalaria glabrata. Comparative Biochemistry and Physiology, v.87B, p.357- 360, 1987. TZOU, P.; REICHHART, J.M.; LEMAITRE, B. Constitutive expression of a single antimicrobial peptide can restore wild-type resistance to infection in immuno-deficient Drosophila mutants. Proceedings of the National Academy of Sciences. USA, v.99, p.2152- 2157, 2002. UNTALAN, P.M.; GUERRERO, F.D.; HAINES, L.R.; PEARSON, T.W. Proteome analysis of abundantly expressed proteins from unfed larvae of the cattle tick, Boophilus microplus. Insect Biochemistry and Molecular Biology, v.35, p.141-151, 2005. VANCE, D.E.; VANCE, J.E. Biochemistry of lipids, lipoproteins and membranes. 4th ed., Vance, D.E.; Vance, J.E. (Eds.), Elsevier Science B.V., 2002. VEY, A.; HOAGLAND, R.E.; BUTT, T.M. Toxic metabolites of fungal control agents. In: Butt, T.M., Jackson, C., Magan, N. (Eds.), Fungi as Biocontrol Agents. CAB International, New York, pp. 311?346, 2001. VIERSTRAETE, E.; VERLEYEN, P.; SAS, F.; VAN DEN BERGH, G.; DE LOOF, A.; ARCKENS, L.; SCHOOFS, L. The instantly released Drosophila immune proteome is 107 infection-specific. Biochemical and Biophysical Research Communications, v.317, p.1052-1060, 2004. VILCINSKAS, A.; MATHA, V.; GOTZ, P. Inhibition of phagocytic activity of plasmatocytes isolated from Galleria mellonella by entomogenous fungi and their secondary metabolites. Journal of Insect Physiology, v.43, p.475-483, 1977. WANG, C.; SKROBEK, A.; BUTT, T.M. Investigations on the destruxin production of the entomopathogenic fungus Metarhizium anisopliae. Journal of Invertebrate Pathology, v.85, n.3, p.168-174, 2004. WANG, H.; NUTTALL, P.A. Comparison of the proteins in salivary glands, saliva and hemolymph of Rhipicephalus appendiculatus female ticks during feeding. Parasitology, v.109, p.517-523, 1994. WEBER, A.N.; TAUSZIG-DELAMASURE, S.; HOFFMANN, J.A.; LELIEVRE, E.; GASCAN, H.; RAY, K.P.; MORSE, M.A.; IMLER, J.L.; GAY, N.J. Binding of the Drosophila cytokine Spatzle to Toll is direct and establishes signaling. Nature Immunology, v.4, p.794-800, 2003. WEERS, P.M.M.; RYAN, R.O. Apolipophorin III: role model apolipoprotein. Insect Biochemistry and Molecular Biology, v.36, p.231?240, 2006. WHARTON, R.H. Acaricide resistance and cattle tick control. Australian Veterinary Journal, v.43, p.394-399, 1967. WHARTON, R.H. Ticks with special emphasis on Boophilus microplus. In: Control of arthropods of medical and veterinary importance. London: Pal, R. & R.H.Wharton (ed.). Plenum Press, 1974. WIGGLESWORTH, V.B. 1967. The principles of insect physiology. Methuen, London, 742p. WINKLER, U.K.; STUCKMANN, M. Glycogen, hyaluronate, and some other polysaccharides greatly enhance the formation of exolipase by Serratia marcescens. Journal of Bacteriology. v.138, p. 663-670, 1979. WOLINS, N.E.; BRASAEMLE, D.L.; BICKEL, P.E. A proposed model of fat packaging by exchangeable lipid droplet proteins. FEBS Letters, v.580, p.5484-5491, 2006. WOOLLEY, T.A. Acarology. Mites and Human Welfare. John Wiley, New York, 1988. 484p. WU, X.; SUN, J.; ZHANG, G.; WANG, H.; NG, T.B. An antifungal defensin from Phaseolus vulgaris cv. ?CloudBean?. Phytomedicine, v.15, n.18, p.104-9, 2011. YASSINE, H.; OSTA, M.A. Anopheles gambiae innate immunity. Cellular Microbiology, v.12, n.1, p.1?9, 2010. YE, J.; ZHAO, H.; WANG, H.; BIAN, J.; ZHENG, R. A defensin antimicrobial peptide from the venoms of Nasonia vitripennis. Toxicon, v.56, p.101-106, 2010. ZHIOUA, E. R.; LEBRUN, A.; JOHNSON, P.W.; GINSBERG, H.S. Ultrastructure of the haemocytes of Ixodes scapularis (Acari: Ixodidae). Acarologia, v.37, p.173-179, 1996. ZHIOUA, E.;. YEH, M.T; LEBRUN, R.A. Assay for pheonloxidase activity in Amblyomma americanum, Dermacentor variabilis, and Ixodes scapularis. Journal of Parasitology, v.83, p.553-554, 1997. 108 ZHU, Y.; THANGAMANI, S.; HO, B.; DING, J.L. The ancient origin of the complement system. The EMBO Journal, v.24, p.382-394, 2005.Pept?deos antimicrobianos; Controle biol?gico; metabolismo lip?dicoCi?ncias Agr?riasAvalia??o dos Perfis Prot?ico e Lip?dico na Resposta de Rhipicephalus Microplus ? 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dc.title.por.fl_str_mv Avalia??o dos Perfis Prot?ico e Lip?dico na Resposta de Rhipicephalus Microplus ? Infec??o com Fungos.
dc.title.alternative.por.fl_str_mv Evaluation of protein and lipid profile in response of Rhipicephalus microplus to infection by fungi.
title Avalia??o dos Perfis Prot?ico e Lip?dico na Resposta de Rhipicephalus Microplus ? Infec??o com Fungos.
spellingShingle Avalia??o dos Perfis Prot?ico e Lip?dico na Resposta de Rhipicephalus Microplus ? Infec??o com Fungos.
Angelo, Isabele da Costa
Pept?deos antimicrobianos; Controle biol?gico; metabolismo lip?dico
Ci?ncias Agr?rias
title_short Avalia??o dos Perfis Prot?ico e Lip?dico na Resposta de Rhipicephalus Microplus ? Infec??o com Fungos.
title_full Avalia??o dos Perfis Prot?ico e Lip?dico na Resposta de Rhipicephalus Microplus ? Infec??o com Fungos.
title_fullStr Avalia??o dos Perfis Prot?ico e Lip?dico na Resposta de Rhipicephalus Microplus ? Infec??o com Fungos.
title_full_unstemmed Avalia??o dos Perfis Prot?ico e Lip?dico na Resposta de Rhipicephalus Microplus ? Infec??o com Fungos.
title_sort Avalia??o dos Perfis Prot?ico e Lip?dico na Resposta de Rhipicephalus Microplus ? Infec??o com Fungos.
author Angelo, Isabele da Costa
author_facet Angelo, Isabele da Costa
author_role author
dc.contributor.advisor1.fl_str_mv Bittencourt, V?nia Rita Elias Pinheiro
dc.contributor.advisor1ID.fl_str_mv 50519867653
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/3888832724995864
dc.contributor.advisor2.fl_str_mv Chagas, Evelize Folly das
dc.contributor.advisor-co1.fl_str_mv Chagas, Evelize Folly das
dc.contributor.referee1.fl_str_mv Fernandes, Everton Kort Kamp
dc.contributor.referee2.fl_str_mv Atella, Georgia Correa
dc.contributor.referee3.fl_str_mv Silva, M?rcia Regina Soares da
dc.contributor.referee4.fl_str_mv Oliveira, Pedro Lagerblad de
dc.contributor.authorID.fl_str_mv 9034779718
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/5028095543336052
dc.contributor.author.fl_str_mv Angelo, Isabele da Costa
contributor_str_mv Bittencourt, V?nia Rita Elias Pinheiro
Chagas, Evelize Folly das
Chagas, Evelize Folly das
Fernandes, Everton Kort Kamp
Atella, Georgia Correa
Silva, M?rcia Regina Soares da
Oliveira, Pedro Lagerblad de
dc.subject.por.fl_str_mv Pept?deos antimicrobianos; Controle biol?gico; metabolismo lip?dico
topic Pept?deos antimicrobianos; Controle biol?gico; metabolismo lip?dico
Ci?ncias Agr?rias
dc.subject.cnpq.fl_str_mv Ci?ncias Agr?rias
description The study evaluated the protein and lipid profiles of Rhipicephalus microplus engorged females after infection by Metarhizium anisopliae, Beauveria bassiana and Fusarium oxysporum. The treatments were immersion or inoculation of conidial suspension in R. microplus. The hemolymph was collected 24 and 48 hours after treatment. The cell-free hemolymph was separated of hemocytes by centrifugation and hemocytes resuspended in phosphate buffer pH 7.2. The amount of total protein was determined in both fractions of hemolymph and hemocytes were quantified. The cell-free hemolymph was filtered through a 100 kDa and 10 kDa membranes, and analyzed by electrophoresis and liquid chromatography (HPLC). The proteome of cell-free hemolymph (treatment by injection) was evaluated by 2DPAGE. Changes were observed in amount total protein and the amount of hemocytes, but no difference was observed in the electrophoretic profile (1D-PAGE) of the cell-free hemolymph. In haemocytes, the entomopathogens reduced the amount of serpins, while F. oxysporum caused increased. In 2D-PAGE variations were observed in both expression and presence/absence of protein between the groups. The cell-free hemolymph antimicrobial activity was tested against Escherichia coli and Staphylococcus aureus and against the fungus used in the treatment of engorged ticks. The hemolymph collected 48 hours after the immersion treatment with B. bassiana apresented activity anti-B. bassiana with 48 hours of evaluation. This hemolymph was subjected to Superose column to HPLC and peak was collected and analyzed on the analytical column C18. The fractions were collected from the C18 and its apresented activity anti-B. bassiana, but showed no activity against Candida albicans. These fractions were analyzed by Maldi-Tof and most of them had in common an ion with m/z 1,119.5; however, other ions may be involved with this activity antimicrobial. The lipids present in cell-free hemolymph, in the hemocyte and fat body were extracted and analyzed by thin layer chromatography (TLC) or HPTLC for neutral lipids and phospholipids. The classes of neutral lipids in the cell-free hemolymph were cholesterol ester, cholesterol (CHO) and fatty acids (FA), which have varied depending on the fungus used, type of treatment and observation time. Phospholipids found were phosphatidylcholine and phosphatidylethanolamine, its were not significantly altered after the fungal infection. In hemocytes, the same classes of lipids were found and B. bassiana modified phospholipids, while M. anisopliae s.l. altered FA and CHO. The fat body showed, in addition to these classes of neutral lipids, the triglycerides, which increased significantly 48 hours after inoculation with M. anisopliae s.l. The lipase activity in fat body was measured and it was demonstrated that increased activity 48 hours after inoculation, mainly in the group inoculated with Metarhizium. Therefore, the results showed alterations related to the proteins expression in the hemocytes and the cell-free hemolymph after inoculation with fungi, immunosuppression of hemocytes and antimicrobial peptides induction after infection with B. bassiana, besides changes in the lipid profile of R. microplus after infection. However, further studies are necessary to understand these changes.
publishDate 2011
dc.date.issued.fl_str_mv 2011-03-03
dc.date.accessioned.fl_str_mv 2016-06-09T16:27:32Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/doctoralThesis
format doctoralThesis
status_str publishedVersion
dc.identifier.citation.fl_str_mv Angelo, Isabele da Costa. Avalia??o dos Perfis Prot?ico e Lip?dico na Resposta de Rhipicephalus Microplus ? Infec??o com Fungos.. 2011. [143 folhas]. Tese( PROGRAMA DE P?S-GRADUA??O EM ADMINISTRA??O) - Universidade Federal Rural do Rio de Janeiro, [Serop?dica/RJ] .
dc.identifier.uri.fl_str_mv https://tede.ufrrj.br/jspui/handle/jspui/1073
identifier_str_mv Angelo, Isabele da Costa. Avalia??o dos Perfis Prot?ico e Lip?dico na Resposta de Rhipicephalus Microplus ? Infec??o com Fungos.. 2011. [143 folhas]. Tese( PROGRAMA DE P?S-GRADUA??O EM ADMINISTRA??O) - Universidade Federal Rural do Rio de Janeiro, [Serop?dica/RJ] .
url https://tede.ufrrj.br/jspui/handle/jspui/1073
dc.language.iso.fl_str_mv por
language por
dc.relation.references.por.fl_str_mv ALVES, S.B. Controle Microbiano de Insetos. 2? ed. Piracicaba: FEALQ, 1998. 1163p. ANGELO, I.C. Avalia??o in vitro dos efeitos de Isaria farinosa, I. fumosorosea, Paecilomyces lilacinus e Lecanicillium lecanii sobre Boophilus microplus. Disserta??o de Mestrado. 37p, 2007. ANGELO, I.C.; FERNANDES, E.K.K.; BAHIENSE, T.C.; PERINOTTO, W.M.S.; MORAES, A.P.R.; TERRA, A.L.M.; BITTENCOURT, V.R.E.P. Efficiency of Lecanicillium lecanii to control the tick Rhipicephalus microplus. Veterinary Parasitology, v.172, p.317- 322, 2010. ARMSTRONG, P.B. The contribution of proteinase inhibitors to immune defense. Trends in Immunology, v.22, p.47-52, 2001. ARRESE, E.L.; HOWARD, A.D.; PATEL, R.T.; RIMOLDI, O.J.; SOULAGES, J.L. Mobilization of lipid stores in Manduca sexta: cDNA cloning and developmental expression of fat body triglyceride lipase, TGL. Insect Biochemistry and Molecular Biology, v.40, p.91-99, 2010. ARRESE, E.L.; SOULAGES, J.L. Insect fat body: energy, metabolism, and regulation. Annual Review of Entomology, v.55, p.207-225, 2010. ASO, Y.; YAMASHITA, T.; MENO, K.; MURAKAMI, M. Inhibition of prophenoloxidaseactivating enzyme from Bombyx mori by endogenous chymotrypsin inhibitors. Biochemistry and Molecular Biology International, v.33, p.751?758, 1994. ATHENSTAEDT, K.; DAUM, G. The life cycle of neutral lipids: synthesis, storage and degradation. Cellular and Molecular Life Sciences, v.63, p.1355-1369, 2006 AUERSWALD, L.; SIEGERT, K.J.; G?DE, G. Activation of triacylglycerol lipase in the fat body of a beetle by adipokinetic hormone. Insect Biochemistry and Molecular Biology, v.35, p.461-470, 2005. AZZOLINI, S.S.; SANTOS, J.M.C.; SOUZA, A.F.; TORQUATO, R.J.S.; HIRATA, I.Y.; ANDREOTTI, R.; TANAKA, A.S. Purification, characterization, and cloning of a serine proteinase inhibitor from the ectoparasite Haematobia irritans irritans (Diptera: Muscidae). Experimental Parasitology, v.106, n.3-4, p.103-109, 2004. BACH?RE, E.; GUEGUEN, Y.; GONZALEZ, M.; LORGERIL, J.; GARNIER, J.; ROMESTAND, B. Insights into the anti-microbial defense of marine invertebrates: the penaeid shrimps and the oyster Crassostrea gigas. Immunological Reviews, v.198, p.149? 168, 2004. BAHIENSE, T. C.; FERNANDES, ?. K. K.; ANGELO, I. C.; PERINOTTO, W. M. S. and BITTENCOURT, V. R. E. P. Performance of Metarhizium anisopliae and its combination with deltamethrin against a pyrethroid-resistant strain of Boophilus microplus in a stall test. Annals of the New York Academy of Sciences, v.1149, p.242-245, 2008. BALASHOV, Yu.S. 1983. An Atlas of Ixodid Tick Ultrastructure. In: Raikhel, A.S., Hoogstral, I.L. (Ed.), Springer, Lanham. 93 BANDSTRA, S.R.; FRIED, B.; SHERMA, J. High-performance thin-layer chromatographic analysis of neutral lipids and phospholipids in Biomphalaria glabrata patently infected with Echinostoma caproni. Parasitology Research, v.99, p.414?418, 2006. BARBAULT, F.; LANDON, C.; GUENNEUGUES, M.; MEYER, J.P.; SCHOTT, V.; DIMARCQ, J.L.; VOVELLE, F. Solution structure of Alo-3: a new knottin-type antifungal peptide from the insect Acrocinus longimanus. Biochemistry, v.42, p.14434-14442, 2003. BARROS, T.A.M.; EVANS, D.E. A??o de gram?neas forrageiras em larvas infectantes do carrapato dos bovinos Boophilus microplus. Pesquisa Veterin?ria Brasileira, v.9, p.17-21, 1989. BEENAKKERS, A.M.T.; VAN DER HORST, D.J.; VANMARREWIJK, W.J.A. Insect flight metabolism. Insect Biochemistry, v.14, p.243-260, 1984. BEISSON, F.; TISS, A.; RIVI?RE, C.; VERGER, R. Methods for lipase detection and assay: a critical review. European Journal of Lipid Science and Technology, v.2, 133-153, 2000. BIDOCHKA, M.J.; SMALL, C.L.; SPIRONELLO, M. Recombination within sympatric cryptic species of the insect pathogenic fungus Metarhizium anisopliae. Environmental Microbiology, v.7, p.1361-1368, 2005. BINNINGTON, K.C; OBENCHAIN, F.D. Structure and function of the circulatory, nervous, and neuroendocrine systems of ticks. In: Obenchain, F.D. and Galun R. (Eds) Physiology of Ticks. Pergamon Press, Oxford, 1982. BISCHOFF, J.F.; REHNER, S.A.; HUMBER, R.A. Metarhizium frigidum sp. nov.: a cryptic species of M. anisopliae and a member of the M. flavoviride complex. Mycologia, v.98, p.737-745, 2006. BISCHOFF, J.F.; REHNER, S.A.; HUMBER, R.A. A multilocus phylogeny of the Metarhizium anisopliae lineage. Mycologia, v.101, p.512-530, 2009. BITTENCOURT, V.R.E.P.; MASCARENHAS, A.G.; FACCINI, J.L.H. Mecanismo de infec??o do fungo Metarhizium anisopliae no carrapato Boophilus microplus em condi??es experimentais. Ci?ncia Rural, Santa Maria, v.29, n.2, p.351-354, 1999. BITTENCOURT, V.R.E.P.; MASSARD, C.L.; LIMA, A.F. Din?mica da infec??o do fungo Metarhizium anisopliae (Metschinikoff, 1879) Sorokin, 1883, sobre o carrapato Boophilus microplus (Canestrini, 1887). Revista Universidade Rural ? S?rie Ci?ncias da Vida, v.17, p.83-88, 1995. BRASAEMLE, D.L. Thematic review series: adipocyte biology. The perilipin family of structural lipid droplet proteins: stabilization of lipid droplets and control of lipolysis. Journal of Lipid Research, v.48, p.2547-2559, 2007. BRAZ, G.R.C.; COELHO, H.S.L.; MASUDA, H.; OLIVEIRA, P.L. A missing metabolic pathway in the cattle tick Boophilus microplus. Current Biology, v.9, n.13, p.703-706, 1999. BROWNBRIDGE, M.; COSTA, S.; JARONSKI, S.T. Effects of in vitro passage of Beauveria bassiana on virulence to Bemisia argentifolii. Journal of Invertebrate Pathology, v.77, p.280-283, 2001. BULET, P.; CHARLET, M.; HETRU, C. In Innate Immunity, (Ezekowitz, R.A.B. and Hoffmann, J.A., Eds.) pp. 89-107. Humana Press, Totowa, NJ. 2003. BULET, P.; HETRU, C.; DIMARQ, J.L.; HOFFMANN, D. Antimicrobial peptides in insects; structure and function. Developmental and Comparative Immunology, v.23, p.329?344, 1999. 94 BULET, P.; STOCKLIN, R. Insect antimicrobial peptides: structure, properties and gene regulation. Protein and Peptide Letters, v.12, p.3-11, 2005. CABRAL, G.A. Lipids as bioeffectors in the immune system. Life Sciences, v.77, p.1699? 1710, 2005. CANAVOSO, L. E.; JOUNI, Z.E.; KARNAS, K.J. PENNINGTON, J.E.; WELLS, M.A. Fat metabolism in insects. Annual Review of Nutrition, v.21, p.23-46, 2001. CASTRILLO, L.A.; ROBERTS, D.W.; VANDENBERG, J.D. The fungal past, present, and future: germination, ramification, and reproduction. Journal of Invertebrate Pathology, v.89, p.46-56, 2005. CASTRO-OCHOA, L.D.; RODRIGUEZ-G?MEZ, C.; VALERIO-ALFARO, G.; ROS, R.O. Screening, purification and characterization of the thermoalkalophilic lipase produced by Bacillus thermoleovorans CCR11. Enzyme and Microbial Technology, v.37, p.648-654, 2005. CERAUL, S.M.; DREHER-LESNICK, S.M.; GILLESPIE, J.J.; RAHMAN, M.S.; AZAD, A.F. New tick defensin isoform and antimicrobial gene expression in response to Rickettsia montanensis challenge. Infection and Immunity, v.75, p.1973?1983, 2007. CHANDLER, D.; DAVIDSON, G.; PELL, J.K.; BALL, B.V.; SHAW, K.; SUNDERLAND, K.D. Fungal biocontrol of acari. Biocontrol Science and Technology, v.10, p.357-384, 2000. CHENG, T.C.; RODRICH, G.E.; FOLEY, D.A.; KOEHLER, S.A. Release of lysozyme from hemolymph cells of Mercenaria mercenaria during phagocytosis. Journal of Invertebrate Pathology, v.25, p.261-265, 1979. CHEON, H.M.; SHIN, S.W.; BIAN, G.; PARK, J.H.; RAIKHEL, A.S. Regulation of lipid metabolism genes, lipid carrier protein lipophorin, and its receptor during immune challenge in the mosquito Aedes aegypti. Journal of Biological Chemistry, v.281, p.8426-8435, 2006. CHINZEI, Y.; CHINO, H.; TAKAHASHI, K. Purification and properties of vitellogenin and vitellin from a tick, Ornithodoros moubata. Journal of Comparative Physiology, v.152B, p.13-21, 1983. CHOI, S.; HWANG, J.M.; KIM, S.I. A Colorimetric Microplate Assay Method for High Throughput Analysis of Lipase Activity. Journal of Biochemistry and Molecular Biology, v.36,p.417-420, 2003. CLARKSON, J.M.; CHARNLEY, A.K. New insights into mechanisms of fungal pathogenesis in insects. Trends Microbiology, v.4, n.5, p.197-203, 1996. COCIANCICH, S.; BULET, P.; HETRU, C.; HOFFMAN, J.A. The inducible antibacterial peptides of insects. Parasitology Today, v.10, p.132-138, 1994. COOP, R.L.; HOLMES, P.H. Nutrition and parasite interactions. International Journal for Parasitology, v.26, p.951-962, 1996. CRUZ, C.E.; FOGA?A, A.C.; NAKAYASU, E.S.; ANGELI, C.B.; BELMONTE, R.; ALMEIDA, I.C.; MIRANDA, A.; MIRANDA, M.T.M.; TANAKA, A.S.; BRAZ, G.R.; CRAIK, C.S.; SCHNEIDER, E.; CAFFREY, C.R.; DAFFRE, S. Characterization of proteinases from the midgut of Rhipicephalus (Boophilus) microplus involved in the generation of antimicrobial peptides. Parasites and Vectors, v.3, n.63, 2010. DA-SILVA VAZ, I.; MARTINEZ, R.H.M.; OLIVEIRA, A.; HECK, A.; LOGULLO, C.; GONZALES, J.C.; DEWES, H.; MASUDA A. Functional bovine immunoglobulins in Boophilus microplus hemolymph. Veterinary Parasitology, 62, 155-160, 1996. 95 DAVIS, B.J. Disc electrophoresis. II. Method and application to human serum proteins Annals of the New York Academy of Sciences, v.121, p.404-27, 1964. DE FARIA, M.R.; WRAIGHT, S.P. Mycoinsecticides and mycoacaricides: a comprehensive list with worldwide coverage and international classification of formulation types. Biological Control, v.43, p.237-256, 2007. DE GREGORIO, E.; SPELLMAN, P.T.; RUBIN, G.M.; LEMAITRE, B. Genome-wide analysis of the Drosophila immune response by using oligonucleotide microarrays. Proceedings of the National Academy of Sciences. USA, v.98, p.12590-12595, 2001. DENARDI, S.E., BECHARA, G.H., CAMARGO-MATHIAS, M.I. New morphological data on fat bodies of semi-engorged females of Amblyomma cajennense (Acari: Ixodidae). Micron, v.36, n.7, p.875-883, 2008. DENARDI, S.E.; BECHARA, G.H.; CAMARGO-MATHIAS, M.I. Fat body cells of Amblyomma cajennense partially engorged females (Acari: Ixodidae) and their role on vitellogenesis process. Experimental Parasitology, v.121, p.213?218, 2009. DENARDI, S.E.; BECHARA, G.H.; OLIVEIRA, P.R.; NUNES, E.T.; SAITO, K.C.; CAMARGO-MATHIAS, M.I. Morphological characterization of the ovary and vitellogenesis dynamics in the tick Amblyomma cajennense (Acari: Ixodidae). Veterinary Parasitology, v.125, p.379?395, 2004. DUMAS, C.; MATHA, V.; QUIOT, J.M.; VEY, A. Effects of destruxins, cyclic depsipeptide mycotoxins, on calcium balance and phosphorylation of intracellular proteins in lepidopteran cell lines. Comparative Biochemistry and Physiology ? Part C: Toxicology and Pharmacology, v.114, p.213?219, 1996. EGGENBERGER, L.R.; LAMORREAUX, W.J.; COONS, L.B. Hemocytic encapsulation of implants in the tick, Dermacentor variabilis. Experimental and Applied Acarology, v.9, p.279-287, 1990. ELEFTHERIANOS, I.; MAROKHAZI, J.; MILLICHAP, P.J.; HODGKINSON, A.J.; SRIBOONLERT, A.; FFRENCH-CONSTANT, R.H.; REYNOLDS, S.E. Prior infection of Manduca sexta with non-pathogenic Escherichia coli elicits immunity to pathogenic Photorhabdus luminescens: roles of immune-related proteins shown by RNA interference. Insect Biochemistry and Molecular Biology, v.36, n.6, p.517?525, 2006. ESTEVES, E.; FOGA?A, A.C.; MALDONADO, R.; SILVA, F.D; MANSO, P.P.; PELAJOMACHADO, M.; VALLE, D.; DAFFRE, S. Antimicrobial activity in the tick Rhipicephalus (Boophilus) microplus eggs: Cellular localization and temporal expression of microplusin during oogenesis and embryogenesis. Development and Comparative Immunology, v.33, n.8, p.913-919, 2009. FAHMY, A.S.; ABDEL-GANY, S.S.; MOHAMED, T.M.; MOHAMED, S.A. Esterase and lipase in camel tick Hyalomma dromedarii (Acari: Ixodidae) during embryogenesis Comparative Biochemistry and Physiology Part B, v.137, p.159?168, 2004. FARGUES, J.; GOETEL, M.S.; SMITS, N.; OUEDRAOGO, A.; VIDAL, C.; LACEY, L.A.; LOMER, C.J.; ROUGIER, M. Variability in susceptibility to simulated sunlight of conidia among isolates of entomopathogenic Hyphomycetes. Mycopathologia, v.135, p.171?181, 1996. FARGUES, J.F.; ROBERT, P.H. Effects of passing through scarabeid hosts on virulence and host specificity of two strains of the entomopathogenic hyphomycete Metarhizium anisopliae. Canadian Journal of Microbiology, v.29, p.576-583, 1983. 96 FEHLBAUM, P.; BULET, P.; MICHAUT, L.; LAGUEUX, M.; BROEKAERT, W. F.; HETRU, C.; HOFFMANNH, J.A. Insect Immunity: Septic injury of Drosophila induces the synthesis of a potent antifungal peptide with sequence homology to plant antifungal peptides. The Journal of Biological Chemistry, v.269, n.52, p.3359-63, 1994. FERNANDES, E.K.K.; BITTENCOURT, V.R.E.P. Entomopathogenic fungi against South American tick species. Experimental and Applied Acarology, v.46. p.71-93, 2008. FERNANDES, ?.K.K.; COSTA, G.L.; MORAES, A.M.L.; BITTENCOURT, V.R.E.P. Entomopathogenic potential of Metarhizium anisopliae isolated from engorged females and tested in eggs and larvae of Boophilus microplus. Journal of Basic Microbiology, v.44, p.270-274, 2004. FERNANDES, ?.K.K.; KEYSER, C.A.; CHONG, J.P.; RANGEL, D.E.N.; MILLER, M.P.; ROBERTS, D.W. Characterization of Metarhizium species and varieties based on molecular analysis, heat tolerance and cold activity. Journal of Applied Microbiology, v.108, p.115-128, 2010. FERRON, P.; FARGUES,J.; RIBA, G. Fungi as microbial insecticides against pests. In: Adora D.K., Ajello L. e Mujerjii K.G. Handbook of Applied Mycology. New York: Marcel Dekker. 1991. FOGA?A, A.C.; ALMEIDA, I.C.; EBERLIN, M.N.; TANAKA, A.S.; BULET, P.; DAFFRE. Ixodidin, a novel antimicrobial peptide from the hemocytes of the catle tick Boophilus microplus with inhibitory activity against serine proteinases. Peptides, v.27, p.667-674, 2006. FOGA?A, A.C.; LORENZINI, D.M.; KAKU, L.M.; ESTEVES, E.; BULET, P.; DAFFRE, S. Cysteine-rich antimicrobial peptides of the cattle tick Boophilus microplus: isolation, structural characterization and tissue expression profile. Developmental and Comparative Immunology, v.28, p.191?200, 2004. FOGA?A, A.C.; SILVA JR, P.I.; MIRANDA, M.T.M.; BIANCHI, A.G.; MIRANDA, A.; RIBOLLA, P.E.M.; DAFFRE, S. Antimicrobial Activity of a Bovine Hemoglobin Fragment in the Tick Boophilus microplus. The Journal of Biological Chemistry, v.274, n.36, 1999. FRAZZON, A.P.G.; JUNIOR, I.S.V.; MASUDA, A.; SCHRANK, A.; VAINSTEIN, M.H. In vitro assessment of Metarhizium anisopliae isolates to control the cattle tick Boophilus microplus. Veterinary Parasitology, v.94, p.117-125, 2000. FURLONG, J. Controle do carrapato dos bovinos na regi?o Sudeste do Brasil. Caderno T?cnico da Escola Veterin?ria UFMG, n.8, p.49-61, 1993. FURLONG, J.; MARTINS, J.R.S.; PRATA, M.C.A. Controle estrat?gico do carrapato dos bovinos. A Hora Veterin?ria, ano 23, n.137, 2004. G?DE, G.; AUERSWALD, L.; MARCO, H.G. Flight fuel and neuropeptidergic control of fuel mobilisation in the twig wilter, Holopterna alata (Hemiptera, Coreidae). Journal of Insect Physiology, v.52, p.1171-81, 2006. GAMS, W.; ROZSYPAL,J. Metarhizium flavoviride n. sp. isolated from insects and soil. Acta Botanica Neerlandica, v.2, p.518-521, 1973. GILBERT, L.I.; CHINO, H. Transport of lipids in insects. Journal of Lipid Research, v.15, p.439-456, 1974. GILLESPIE, J.P.; KANOST, M.R.; TRENCZEK, T. Biological mediators of insect immunity. Annual Review of Entomology, v.42, p.611-643, 1997. 97 GINDIN, G.; SAMISH, M.; ALEKSEEV, E.; GLAZER, I. The Susceptibility of Boophilus annulatus (Ixodidae) Ticks to Entomopathogenic Fungi. Biocontrol Science and Technology, v.11, p.111-118, 2001. GOBERT, V.; GOTTAR, M.; MATSKEVICH, A.A.; RUTSCHMANN, S.; ROYET, J.; BELVIN, M.; HOFFMANN, J.A.; FERRANDON, D. Dual activation of the Drosophila Toll pathway by two pattern recognition receptors. Science, v.302, p.2126-2130, 2003. GOLODNE, D.M.; MONTEIRO, R.Q.; GRA?A-SOUZA, A.V.; SILVA-NETO, M.A.C.; ATELLA, G.C. Lysophosphatidylcholine acts as na anti-hemostatic molecule in the saliva of the blood-sucking bug Rhodnius prolixus. Journal of Biological Chemistry, v.278, n.30, p.2766-2771, 2003. GONDIM, K.C.; OLIVEIRA, P.L.; COELHO, H.S.L.; MASUDA, H. Lipophorin from Rhodnius prolixus: purification and partial characterization. Insect Biochemistry, v.19, p.153-161, 1989. GONZALES, J. C. O controle do carrapato dos bovinos. Porto Alegre: Sulina, 1975. 103p. GONZALES, J.C. O carrapato do boi: vida, resist?ncia e controle. S?o Paulo: Mestre Jou, 1974. 101p. GONZ?LES, J.C. O carrapato dos bovinos Boophilus microplus (Can. 1887) (Revis?o hist?rica e conceitual). A Hora Veterin?ria, ano 21, n.125, p.23-28, 2002 GORMAN, M.J.; PASKEWITZ, S.M. Serine proteases as mediators of mosquito immune responses. Insect Biochemistry and Molecular Biology, v.31, p.257?62, 2001. GOTTAR, M.; GOBERT, V.; MATSKEVICH, A.; REICHHART, J.M.,; WANG, C.; BUTT, T.M.; BELVIN, M.; HOFFMANN, J.A.; FERRANDON, D. Dual detection of fungal infections in Drosophila by recognition of glucans and sensing of virulence factors. Cell, v.127, p.1425-1437, 2006. GRISI, L.; MASSARD, C.L.; BORJA, G.E.M.; PEREIRA, J.B. Impacto econ?mico das principais ectoparasitoses em bovinos no Brasil. A Hora Veterin?ria, ano 21, n.125, p.23-28, 2002. GRUBHOFFER, L.; VERES, J.; DUSBABEK, F. Lectins as the molecular factors of recognition and defense reactions of ticks. In: Dusbabek, F., Bukva, V. (Eds.), Modern Acarology, 2. Academia, Prag, p.381-388, 1991. GUDDERRA, N.P.; NEESE, P.A.; SONENSHINE, D.E.; APPERSON, C.S.; ROE, R.M. Developmental profile, isolation, and biochemical characterization of a novel lipoglycohemecarrier protein from the American dog tick, Dermacentor variabilis (Acari:Ixodidae) and observations on a similar protein in the soft tick, Ornithodoros parkeri (Acari:Argasidae). Insect Biochemistry and Molecular Biology, v.31, p.299-311, 2001. GUDDERRA, N.P.; SONENSHINE, D.E.; APPERSON, C.S.; ROE, R.M. Hemolymph proteins in ticks. Journal of Insect Physiology, v.48, p.269-278, 2002a. GUDDERRA, N.P.; SONENSHINE, D.E.; APPERSON, C.S.; ROE, R.M. Tissue distribuition and characterization of predominant hemolymph carrier proteins from Dermacentor variabilis and Ornithodoros parkeri. Journal of Insect Physiology, v.48, p.19- 29, 2002b. GUIMAR?ES, A.M.; LIMA, J.D.; RIBEIRO, M.F.B. Sporogony and experimental transmission of Babesia equi by Boophilus microplus. Parasitology Research, v.84, p.323- 327, 1998 98 HANCOCK, R.E.W.; BROWN, K.L.; MOOKHERJEE, N. Host defence peptides from invertebrates ? emerging antimicrobial strategies. Immunobiology, v.211, p.315-322, 2006. HANCOCK, R.E.W.; LEHRER, R. Cationic peptides: a new source of antibiotics. Trends in Biotechnology, v.16, p.82?88, 1998. HANCOCK, R.E.W.; SCOTT, M.G. The role of antimicrobial peptides in animal defenses. Proceedings of the National Academy of Sciences, v.97, p.8856-8861, 2000. HAWKSWORTH, D.L. Micologist?s handbook. 2?ed. England, Kew Surrey: CAB Press, 1977. 231p HAWKSWORTH, D.L.; KIRK, P.M.; SUTTON, B.C.; PEGLER, D.N. ( Ainsworth and Bisby?s) Dictionary of the Fungi, 8?ed. , CAB International, Wallingford, UK, 616p., 1995. HETRU, C.; HOFFMAN, D.; BULET, P. Antimicrobial peptides from insects. In: Brey, P.T., Hultmark, D. (Eds.), Molecular mechanisms of immune responses in insects. Chapman and Hall, New York, 1998. HEUCHERT, C.M.; DE, G.V.JR.; DE ATHAIDE, D.F.; BOSE, R.; FRIEDHOFF, K.T. Seroepidemiologic studies on Babesia equi and Babesia caballi infections in Brazil. Veterinary Parasitology, v.85, p.1-11, 1999. HOFFMANN, J.A. The immune response of Drosophila. Nature, v.426, p.33-38, 2003. HOFFMANN, J.A.; KATATOS, F.C.; JANEWAY, C.A.JR.; EZEKOWITZ, R.A.B. Phylogenetic perspectives in innate immunity. Science, v.284, p.1313-1318, 1999. HOFFMANN, J.A.; REICHHART, J.M. Drosophila innate immunity: an evolutionary perspective. Nature Immunology, v.3, n.2, 2002. HORN, S.C.; ARTECHE, C.C.P. Situa??o parasit?ria da pecu?ria no Brasil. A Hora Veterin?ria, v.4, p.12-32, 1985. HORWITZ, J.; PERLMAN, R.L. Measurement of inositol phospholipid-metabolism in PC12 pheochromocytoma cells. Methods in Enzymology, v.141, p.169-175, 1987. HU, Q.B.; REN, S.X.; WU, J.H.; CHANG, J.M.; MUSA, P.D. Investigation of destruxin A and B from 80 Metarhizium strains in China, and the optimization of cultural conditions for the strain MaQ10. Toxicon, v.48, p.491-498, 2006. HUNT, D.W.A. Absence of fatty acid germination inhibitors for conidia of Beauveria bassiana on the integument of the bark beetle Dendroctonus ponderosae (Coleoptera: Scolytidae). The Canadian Entomologist, v.118, p.837-838, 1986. IWANAGA S. The molecular basis of innate immunity in the horseshoe crab. Current Opinion in Immunology, v.14, p.87?95, 2002. IWANAGA, S.; LEE, B.L. Recent advances in the innate immunity of invertebrates animals. Insect Biochemistry and Molecular Biology, v.38, p.128-150, 2005. JAEGER, K.E.; EGGERT, T. Lipases for biotechnology. Current Opinion in Biotechnology, v.13, p.390?397, 2002. JAMES, A.M.; OLIVER, J.H. Vitellogenesis and its hormonal regulation in Ixodida: preliminary results. In: Needhan, G.R., Mitchell, R., Horn, D.J., Welbourn, W.C. (Eds), Acarology IX, v.2. Ohio Biological Survey, Columbus, Ohio, 1999. JIANG, H.; KANOST, M.R. The clip-domain family of serine proteinases in arthropods. Insect Biochemistry and Molecular Biology, v.30, p.95-105, 2000. 99 JOHNS, R.; OHNISHI, J.; BROADWATER, A.; SONENSHINE, D.E.; DE SILVA, A.M.; HYNES, W.L. Contrasts in tick innate immune responses to Borrelia burgdorferi challenge: immunotolerance in Ixodes scapularis versus immunocompetence in Dermacentor variabilis (Acari: Ixodidae). Journal of Medical Entomology 38:99?107, 2001a. JOHNS, R.; SONENSHINE, D. E.; HYNES, W. L Control of bacterial infections in the hard tick Dermacentor variabilis (Acari: Ixodidae): evidence for the existence of antimicrobial proteins in tick hemolymph. Journal of Medical Entomology, v. 35, n.4, p. 458-464, 1998. JOHNS, R.; SONENSHINE, D.E.; HYNES, W.L. Identification of a defensin from the hemolymph of the American dog tick, Dermacentor variabilis. Insect Biochemistry and Moecular Biology, v.31, p.857?865, 2001b. JURETIC, D.; VUKICEVIC, D.; ILIC, N.; ANTCHEVA, N.; TOSSI, A. Computational design of highly selective antimicrobial peptides. Journal of Chemical Information and Modeling, v.49, p.2873?2882, 2009. KABARA, J.J.; SWIECZKOWSKI, D.M.; CONLEY, A.J.; TRUANT, J.P. Fatty acids and derivatives as antimicrobial agents. Antimicrobial Agents and Chemotherapy, v.2, n.1, p.23-28, 1972. KADOTA, K.; SATOH, E.; OCHIAI, M.; INOUE, N.; TSUJI, N.; IGARASHI, I.; NAGASAWA, N.; MIKAMI, T.; CLAVERIA, F.G.; FUJISAKI, K. Existence of phenol oxidase in the argasid tick Ornithodoros moubata. Parasitology Research, v.88, p.78-784, 2002. KANOST, M.R. Serine proteinase inhibitors in arthropod immunity. Developmental and Comparative Immunology, v.23, p.291-301, 1999. KANOST, M.R.; JIANG, H.; Yu, X.Q. Innate immune responses of a lepidopteran insect, Manduca sexta. Immunological Reviews, v.198, p.97?105, 2004. KAUFMAN, W.R.; PHILLIPS, J.E. Ion and water balance in the ixodid tick, Dermacetor andersoni. I. Routes of ion and water excretion. Journal of Experimental Biology, 58, p.523-536, 1973. KAWOOYA, J. K.; LAW, J.H. Role of lipophorin in lipid transport to insect egg. The Journal of Biological Chemistry, v.263, p.8748-8753, 1988. KEELEY, L.L. Comprehensive Insect Physiology, Biochemistry and Pharmacology, Physiology and biochemistry of the fat body. In: Kerkut, G.A. and Gilbert, L.I. (Eds) 1985 v.3. Integument, Respiration and Circulation. Pergamon Press, Oxford. 1985. KLAUDINY, J.; ALBERT, S.; BACHANOVA, K.; KOPERNICKY, J.; SIMUTH, J. Two structurally different defensin genes, one of them encoding a novel defensin isoform, are expressed in honeybee Apis mellifera. Insect Biochemistry and Molecular Biology, v.35, p.11-22, 2005. KONGSUWAN, K.; JOSH, P.; ZHU, Y.; PEARSON, R.; GOUGH, J.; COLGRAVE, M.L. Exploring the midgut proteome of partially fed female cattle tick (Rhipicephalus (Boophilus) microplus). Journal of Insect Physiology, v.56, p.212-226, 2010. KOPACEK, P.; WEISE, C.; SARAVANAN, T.; VITOVA, K.; GRUBHOFFER, L. Characterization of an -macroglobulin-like glycoprotein isolated from the plasma of the soft tick Ornithodoros moubata. European Journal of Biochemistry, v.267, p.465-475, 2000. 100 KOVAR, V.; KOPACEK, P.; GRUBHOFFER, L. Characterization of an -macroglobulinlike glycoprotein isolated from the plasma of the soft tick Ornithodoros moubata. European Journal of Biochemistry, v.267, p.465-475, 2000. KUHN, K.H.; HAUG, T. Ultrastructure, cytochemical and immunocytochemical characterization of hemocytes of the hard tich Ixodes ricinus (Acari: Chelicerata). Cell and Tissue Research, v.277, p.493-504, 1994. KUROOKA, S.; OKAMOTO, S.; HASHIMOTO, M. A novel and simple colorimetric assay for human serum lipase. The Journal of Biochemistry, v.81, p.361-369, 1977. LAEMMLI, U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, v.227, p.680?685, 1970. LAMBERTY, M.; ZACHARY, D.; LANOT, R.; BORDEREAU, C.; ROBERT, A.; HOFFMANN, J.A.; BULET, P. Constitutive expression of a cysteine-rich antifungal and a linear antibacterial peptide in a termite insect. Journal Biological Chemistry, v.276, p.4085- 4092, 2001. LAMPROU, I.; MAMALI, I.; DALLAS, K.; FERTAKIS, V.; LAMPROPOULOU, M.; MARMARAS, V.J. Distinct signalling pathways promote phagocytosis of bacteria, latex beads and lipopolysaccharide in medfly haemocytes. Immunology, v.121, n.3, p.314-327, 2007. LAVINE, M.D.; STRAND, M.R. Haemocytes from Pseudomonas includens express multiple and integrin subunits. Insect Molecular Biology, v.12, n.5, p.441-452, 2003. LEE, H.; KWON, H.M.; PARK, J.W.; KUROKAWA, K.; LEE, B.L. N-terminal GNBP homology domain of Gram-negative binding protein 3 functions as a beta-1,3-glucan binding motif in Tenebrio molitor. Biochemistry and Molecular Biology Reports, v.42, p.506-510, 2009. LEMAITRE, B. The road to Toll. Nature Reviews Immunology, v.4, p.521?527, 2004. LEMAITRE, B.; HOFFMANN, J. The host defense of Drosophila melanogaster Annual Review of Immunology, v.25, p.697?743, 2007. LEMAITRE, B.; REICHHART, J.; HOFFMANN, J. Drosophila host defense: differential induction of antimicrobial peptide genes after infection by various classes of microorganisms. Proceedings of the National Academy of Sciences. USA, v.94, p.14614-14619, 1997. LEVASHINA, E.A. Innate immune response of Anopheles gambiae. Insect Biochemistry and Molecular Biology, v.34, p.673-678, 2004. LEVASHINA, E.A.; LANGLEY, E.; GREEN, C.; GUBB, D.; ASHBURNER, M.; HOFFMANN, J.A.; REICHHART, J.M. Constitutive activation of toll mediated antifungal defense in serpin-deficient Drosophila. Science, v.285, p.1917-1919, 1999. LEVY, F.; BULET, P.; EHRET-SABATIER, L. Proteomic analysis of the systemic immune response of Drosophila. Molecular and Cellular Proteomics, v.3, p.156-166, 2004. LIMA, C.A.; TORQUATO, R.J.S.; SASAKI, S.D.; JUSTO, G.Z.; TANAKA, A.S. Biochemical characterization of a Kunitz type inhibitor similar to dendrotoxins produced by Rhipicephalus (Boophilus) microplus (Acari: Ixodidae) hemocytes. Veterinary Parasitology, v.167, p.279-287, 2010. LOHNER, K. The role of membrane lipid composition in cell targeting of antimicrobial peptides. Development of novel antimicrobial agents: emerging strategies. Lohner, K. (ed), Horizon Scientific Press, England, p.149-165, 2001. 101 LOMER, C.J.; BATEMAN, R.P.; JOHNSON, D.L.; LANGEWALD, J.; THOMAS, M. Biological control of locusts and grasshoppers. Annual Review of Entomology, v.46, p.667- 702, 2001. LOPES, R.B.; ALVES, S.B.; PADULLA, L.F.L.; P?REZ, C.A. Efici?ncia de formula??es de Beauveria bassiana and Metarhizium anisopliae para o controle de ninfas de Amblyomma cajennense (FABRICIUS, 1787). Revista Brasileira de Parasitologia Veterin?ria, v.16, p.27?31, 2007. LOWENBERGER, C.; BULET, P.; CHARLET, M.; HETRU, C.; HODGEMAN, B.; CHRISTENSEN, B.M.; HOFFMAN, J.A. Insect immunity: isolation of three novel inducible antimicrobial defensins from the vector mosquito, Aedes aegypt. Insect Biochemistry and Molecular Biology, v.25, p.867-873, 1995. LU, Y.; LU, F.; WANG, X.; BIE, X.; SUN, H.; WUYUNDALAI,; LU, Z. Identification of bacteria producing a thermophilic lipase with positional non-specificity and characterization of the lipase. Annals of Microbiology, v.59, n.3, p.565-571, 2009. LUBECK, I.; ARRUDA, W.; SOUZA, B.K.; STANIS?UASKI, F.; CARLINI, C.R.; SCHRANK, A.; VAINSTEIN, M.H. Evaluation of Metarhizium anisopliae strains as potential biocontrol agents of the tick Rhipicephalus (Boophilus) microplus and the cotton stainer Dysdercus peruvianus. Fungal Ecology, v.1, p.78-88, 2008. LUO, T.; ZHANG, X.; SHAO, Z.; XU, X. PmAV, a novel gene involved in virus resistance of shrimp Penaeus monodon. FEBS Letters, v.551, p.53-57, 2003. LUZ, C.; TIGANO, M.S.; SILVA, I.G.; CORDEIRO, C.M.T.; ALJANABI, S.M. Selection of Beauveria bassiana and Metarhizium anisopliae isolates to control Triatoma infestans. Mem?rias do Instituto Oswaldo Cruz, v.93, n.6, p.839-846, 1998. MANZANO-ROMAN, R.; ALMAZ?N, C.; NARANJO, V.; BLOUIN, E.F.; KOCAN, K.M.; DE LA FUENTE, J. Expression of perilipin in human promyelocytic cells in response to Anaplasma phagocytophilum infection results in modified lipid metabolism. Journal of Medical Microbiology, v.57,p.159-163, 2008. MARKWELL, M.A.K.; HAAS, S.M.; BIEBER, L.L.; TOLBERT, N.E. A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samples. Analitical Biochemistry, v.87, p.206?210, 1978. MARMARAS. V.J.; LAMPROPOULOU, M. Regulators and signalling in insect haemocyte immunity. Cellular Signalling, v.21, p.186-195, 2009. MARTIN, H.S.; TIMOTHY, S.; MANICKAM, S.; KENNETH, S.; JOHN, H.L. Proenzyme of Manduca sexta phenoloxidase: purification, activation, substrate specificity of the active enzyme, and molecular cloning. Proceeding of the National Academy of Science, USA, v.92, p.7764-7768, 1995. MAYA-MONTEIRO, C.M.; DAFFRE, S.; LOGULLO, C.; LARA, F.A.; ALVES, E.W.; CAPURRO, M.L.; ZINGALI, R.; ALMEIDA, I.C.; OLIVEIRA, P.L. HeLp, a Heme Lipoprotein from the hemolymph of the catlle tick, Boophilus microplus. The Journal of Biological Chemistry, v.275, n.47, p.36584-36589, 2000. MEDZHITOV, R
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