Avalia??o hematol?gica, bioqu?mica, cl?nica e diagn?stico molecular de agentes micoplasmas hemotr?ficos em c?es dom?sticos oriundos de abrigos da regi?o metropolitana do Rio de Janeiro

• Autor(a) principal: Silva, Aline Tonussi da
• Data de Publicação: 2016
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Biblioteca Digital de Teses e Dissertações da UFRRJ
• Texto Completo: https://tede.ufrrj.br/jspui/handle/jspui/1430
• topic: Domestic dogs; Hemoplasmas; Shelters; C?es dom?sticos; Hemoplasmas; Abrigos.; Medicina Veterin?ria

The hemotropic hemoparasites mycoplasmas are gram-negative bacteria, which must attach to the wall of erythrocytes, causing deformation in the membrane of the infected erythrocytes. The goal of this study was to detect molecularly hemoplasmas infection in sheltered dogs in Metropolitan region of Rio de Janeiro, linking it to hematological, biochemical and clinical data changes. Of 222 dogs sampled, 20,27% (45/222) were positive by the Polymerase Chain Reaction Real Time (qPCR) for Mycoplasma spp.. The frequency of hemoplasma species infection by PCR using species-specific oligonucleotides, was 13,06% (29/222), 0% (0/222) and 2,25% (5/222) respectively for M. haemocanis (Mhc), ?Candidatus M. turicensis? (Cmt) and ?Candidatus Mycoplasma haematoparvum / haemominutum? (Cmhp / m). There for e M. haemocanis presented the highest incidence in the dogs population studied. Coinfections were identified between Cmhp/m and Mhc 1,35% (3/222). Regarding hematological alterations associated with infection by Mycoplasma spp., hyperproteinemia on plasma was detected (p = 0,0010). It was not observed an association between hemoplasmas infection and the occurrence of anemia and leukocyte changes. Changes in serum biochemistry showed a significant difference in serum protein (p = 0,0291), globulin (p = 0,0253), creatinine (p = 0,0279) and alkaline phosphatase (p = 0,0242). Additionally the azotemia was statistically (p = 0,0023) associated with the infection. The results obtained in the evaluation of Mhc positive dogs demonstrated no significant difference in plasma total protein (p = 0,0012) and hyperproteinaemia was associated with infection (p = 0,0238). There were significant differences in alkaline phosphatase parameter (p = 0,0173) and urea (p = 0,0401), with azotemia is associated shall infection (p = 0,0061). The results observed in the evaluation of positive dogs for (Cmhp / m) show that ALT (alanine aminotransferase) was associated with infection (p = 0,0301). As for factors associated with infection by Mycoplasma spp., only gender (p = 0,0062) and age (p = 0,0041) were associated with infec??o.N?o are statistically associated with the historical agent of fights, access to street, castration, history fleas, ticks history, presence of fleas and ticks. The frequency of the main findings of anamanse and clinical examination of the dogs positive for Mycoplasma spp . were lymphadenopathy (37,8% ) , skin lesions compatible with scabies ( 28,9%) , TPC > 2 ( 31,1% ) , oral affections ( 15,5%) of hist?rico ehrlichiosis ( 13,3%) . Consequently, knowledge of the disease and the factors related to their occurrence are necessary for the adoption of preventive measures aimed at animal health.