Caracteriza??o molecular (RAPD) e an?lise das prote?nas de reserva em gr?os de variedades locais de arroz do Maranh?o
Autor(a) principal: | |
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Data de Publicação: | 2006 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da UFRRJ |
Texto Completo: | https://tede.ufrrj.br/jspui/handle/jspui/1235 |
Resumo: | Brazil is the country with the greatest plant genetic diversity in the world with the potential still underestimated what makes it necessary to characterize local genetic material. Genetic resources are studie in several stages and identify traits of agronomic value in hardy varieties is one of the roles of gene banks (BAG's). In rice, because it is a very important crop for the diet of the brazilian population, selecting genotypes with high yield, nutritional value and adapted to the most diverse environmental conditions is a continuous search for improvement programs. In this sense, the present study aimed to adapt methodologies for protein extraction reserve and DNA of rice seeds to be used in routine laboratory for characterization of germplasm banks. After optimization of methodologies, seeds of twenty local varieties of rice grown in the state of Maranh?o different times (Experiment I: Aug-2002 to Mar-2003 and Experiment II: Nov-2002 to Jun-2003) were analyzed for study of diversity genetic (DNA and protein) and seasonality effect on its accumulation of reserves and gross protein. The analysis of variance or protein content and protein fractions was highly significant (P <0.01) between genotypes and experiments. The mean protein fractions albumin+globulin and glutelin were higher in experiment I, whose average temperatures were 330C while the average gross protein (CP) was higher than in Experiment II in which the average temperature was 410C.Varieties analyzed, the Pingo d? ?gua (220019) and Jatob? (220012) showed the highest values for PB (11.32 and 11.13%) and glutelin (83.73 and 92.86 mg flour). The molecular characterization by RAPD was based on 37 polymorphic band sand phylogenetic analysis revealed a genetic diversity at 50% training with four groups and three separate varieties. Groups based on the varieties named Lageado and Zebu branco seems to have been the shape of the grains and reason C/L. |
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Fernandes, Manlio Silvestre002.180.573-34Souza, Sonia Regina deRumjanek, Norma Gouv?aMagalh?es, Jos? RonaldoXavier, Gustavo Ribeiro625.689.143-00http://lattes.cnpq.br/9812417823785807Ara?jo, Elisangela Sousa de2016-10-04T19:57:29Z2006-02-23ARA?JO, Elisangela Sousa de. Caracteriza??o molecular (RAPD) e an?lise das prote?nas de reserva em gr?os de variedades locais de arroz do Maranh?o. 2006. 44 f. Tese (Doutorado em Agronomia - Ci?ncia do Solo) - Instituto de Agronomia, Departamento de Solos, Universidade Federal Rural do Rio de Janeiro, Serop?dica - RJ, 2014.https://tede.ufrrj.br/jspui/handle/jspui/1235Brazil is the country with the greatest plant genetic diversity in the world with the potential still underestimated what makes it necessary to characterize local genetic material. Genetic resources are studie in several stages and identify traits of agronomic value in hardy varieties is one of the roles of gene banks (BAG's). In rice, because it is a very important crop for the diet of the brazilian population, selecting genotypes with high yield, nutritional value and adapted to the most diverse environmental conditions is a continuous search for improvement programs. In this sense, the present study aimed to adapt methodologies for protein extraction reserve and DNA of rice seeds to be used in routine laboratory for characterization of germplasm banks. After optimization of methodologies, seeds of twenty local varieties of rice grown in the state of Maranh?o different times (Experiment I: Aug-2002 to Mar-2003 and Experiment II: Nov-2002 to Jun-2003) were analyzed for study of diversity genetic (DNA and protein) and seasonality effect on its accumulation of reserves and gross protein. The analysis of variance or protein content and protein fractions was highly significant (P <0.01) between genotypes and experiments. The mean protein fractions albumin+globulin and glutelin were higher in experiment I, whose average temperatures were 330C while the average gross protein (CP) was higher than in Experiment II in which the average temperature was 410C.Varieties analyzed, the Pingo d? ?gua (220019) and Jatob? (220012) showed the highest values for PB (11.32 and 11.13%) and glutelin (83.73 and 92.86 mg flour). The molecular characterization by RAPD was based on 37 polymorphic band sand phylogenetic analysis revealed a genetic diversity at 50% training with four groups and three separate varieties. Groups based on the varieties named Lageado and Zebu branco seems to have been the shape of the grains and reason C/L.O Brasil ? o pa?s com a maior diversidade gen?tica vegetal do mundo com potencial ainda subestimado o que torna fundamental a caracteriza??o do material gen?tico local. Os recursos gen?ticos s?o estudados em v?rias etapas e identificar caracter?sticas de valor agron?mico em variedades r?sticas ? um dos pap?is dos bancos de germoplasma (BAG?s). Em arroz, por se tratar de uma cultura muito relevante para a dieta da popula??o brasileira, selecionar gen?tipos com alta produtividade, valor nutricional e adaptados ?s mais diversificadas condi??es ambientais ? uma busca cont?nua em programas de melhoramento. Nesse sentido, o presente trabalho teve por objetivos adaptar metodologias para extra??o de prote?na de reserva e de DNA de sementes de arroz a fim de serem empregadas na rotina de laborat?rio para caracteriza??o de bancos de germoplasma. Ap?s otimiza??o das metodologias, sementes de vinte variedades locais de arroz do Estado do Maranh?o cultivadas em ?pocas distintas (Experimento I: Ago-2002 a Mar-2003 e Experimento II: Nov-2002 Jun-2003) foram analisadas para fins de estudo de diversidade gen?tica (DNA e prote?na) e efeito da sazonalidade em seu ac?mulo de prote?na bruta e reserva. A an?lise de vari?ncia para teor de prote?na e fra??es proteicas foi altamente significativa (P<0,01) entre os gen?tipos, e os experimentos. As m?dias das fra??es proteicas albumina+globulina e glutelina foram superiores no experimento I, cujas temperaturas m?dias foram de 330C enquanto que a m?dia da prote?na bruta (PB) foi superior no Experimento II em que a m?dia de temperatura foi de 410C. Das variedades analisadas, a Pingo d??gua (220019) e Jatob? (220012) foram as que apresentaram os maiores valores para PB (11,32 e 11,13%) e glutelina (83,73 e 92,86 mg.g farinha). A caracteriza??o molecular pela RAPD foi baseada em 37 bandas polim?rficas e a an?lise filogen?tica revelou uma diversidade gen?tica em 50% com forma??o de quatro grupos e tr?s variedades isoladas. Os grupos formados pelas variedades de nome Lageado e Zebu branco parecem ter sido pela forma dos gr?os e raz?o C/L.Submitted by Jorge Silva (jorgelmsilva@ufrrj.br) on 2016-10-04T19:57:29Z No. of bitstreams: 1 2006 - Elisangela Sousa de Araujo.pdf: 1061759 bytes, checksum: 865d0167431190cf5627ccdf21cf91fb (MD5)Made available in DSpace on 2016-10-04T19:57:29Z (GMT). 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dc.title.por.fl_str_mv |
Caracteriza??o molecular (RAPD) e an?lise das prote?nas de reserva em gr?os de variedades locais de arroz do Maranh?o |
dc.title.alternative.eng.fl_str_mv |
Molecular characterization (SSR and RAPD) and analysis of storage proteins in grains of local varieties of rice of the Maranh?o |
title |
Caracteriza??o molecular (RAPD) e an?lise das prote?nas de reserva em gr?os de variedades locais de arroz do Maranh?o |
spellingShingle |
Caracteriza??o molecular (RAPD) e an?lise das prote?nas de reserva em gr?os de variedades locais de arroz do Maranh?o Ara?jo, Elisangela Sousa de DNA extaction Protein extraction Molecular markers Extra??o de DNA Extra??o de prote?nas Marcadores moleculares Agronomia |
title_short |
Caracteriza??o molecular (RAPD) e an?lise das prote?nas de reserva em gr?os de variedades locais de arroz do Maranh?o |
title_full |
Caracteriza??o molecular (RAPD) e an?lise das prote?nas de reserva em gr?os de variedades locais de arroz do Maranh?o |
title_fullStr |
Caracteriza??o molecular (RAPD) e an?lise das prote?nas de reserva em gr?os de variedades locais de arroz do Maranh?o |
title_full_unstemmed |
Caracteriza??o molecular (RAPD) e an?lise das prote?nas de reserva em gr?os de variedades locais de arroz do Maranh?o |
title_sort |
Caracteriza??o molecular (RAPD) e an?lise das prote?nas de reserva em gr?os de variedades locais de arroz do Maranh?o |
author |
Ara?jo, Elisangela Sousa de |
author_facet |
Ara?jo, Elisangela Sousa de |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Fernandes, Manlio Silvestre |
dc.contributor.advisor1ID.fl_str_mv |
002.180.573-34 |
dc.contributor.advisor-co1.fl_str_mv |
Souza, Sonia Regina de |
dc.contributor.referee1.fl_str_mv |
Rumjanek, Norma Gouv?a |
dc.contributor.referee2.fl_str_mv |
Magalh?es, Jos? Ronaldo |
dc.contributor.referee3.fl_str_mv |
Xavier, Gustavo Ribeiro |
dc.contributor.authorID.fl_str_mv |
625.689.143-00 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/9812417823785807 |
dc.contributor.author.fl_str_mv |
Ara?jo, Elisangela Sousa de |
contributor_str_mv |
Fernandes, Manlio Silvestre Souza, Sonia Regina de Rumjanek, Norma Gouv?a Magalh?es, Jos? Ronaldo Xavier, Gustavo Ribeiro |
dc.subject.eng.fl_str_mv |
DNA extaction Protein extraction Molecular markers |
topic |
DNA extaction Protein extraction Molecular markers Extra??o de DNA Extra??o de prote?nas Marcadores moleculares Agronomia |
dc.subject.por.fl_str_mv |
Extra??o de DNA Extra??o de prote?nas Marcadores moleculares |
dc.subject.cnpq.fl_str_mv |
Agronomia |
description |
Brazil is the country with the greatest plant genetic diversity in the world with the potential still underestimated what makes it necessary to characterize local genetic material. Genetic resources are studie in several stages and identify traits of agronomic value in hardy varieties is one of the roles of gene banks (BAG's). In rice, because it is a very important crop for the diet of the brazilian population, selecting genotypes with high yield, nutritional value and adapted to the most diverse environmental conditions is a continuous search for improvement programs. In this sense, the present study aimed to adapt methodologies for protein extraction reserve and DNA of rice seeds to be used in routine laboratory for characterization of germplasm banks. After optimization of methodologies, seeds of twenty local varieties of rice grown in the state of Maranh?o different times (Experiment I: Aug-2002 to Mar-2003 and Experiment II: Nov-2002 to Jun-2003) were analyzed for study of diversity genetic (DNA and protein) and seasonality effect on its accumulation of reserves and gross protein. The analysis of variance or protein content and protein fractions was highly significant (P <0.01) between genotypes and experiments. The mean protein fractions albumin+globulin and glutelin were higher in experiment I, whose average temperatures were 330C while the average gross protein (CP) was higher than in Experiment II in which the average temperature was 410C.Varieties analyzed, the Pingo d? ?gua (220019) and Jatob? (220012) showed the highest values for PB (11.32 and 11.13%) and glutelin (83.73 and 92.86 mg flour). The molecular characterization by RAPD was based on 37 polymorphic band sand phylogenetic analysis revealed a genetic diversity at 50% training with four groups and three separate varieties. Groups based on the varieties named Lageado and Zebu branco seems to have been the shape of the grains and reason C/L. |
publishDate |
2006 |
dc.date.issued.fl_str_mv |
2006-02-23 |
dc.date.accessioned.fl_str_mv |
2016-10-04T19:57:29Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
ARA?JO, Elisangela Sousa de. Caracteriza??o molecular (RAPD) e an?lise das prote?nas de reserva em gr?os de variedades locais de arroz do Maranh?o. 2006. 44 f. Tese (Doutorado em Agronomia - Ci?ncia do Solo) - Instituto de Agronomia, Departamento de Solos, Universidade Federal Rural do Rio de Janeiro, Serop?dica - RJ, 2014. |
dc.identifier.uri.fl_str_mv |
https://tede.ufrrj.br/jspui/handle/jspui/1235 |
identifier_str_mv |
ARA?JO, Elisangela Sousa de. Caracteriza??o molecular (RAPD) e an?lise das prote?nas de reserva em gr?os de variedades locais de arroz do Maranh?o. 2006. 44 f. Tese (Doutorado em Agronomia - Ci?ncia do Solo) - Instituto de Agronomia, Departamento de Solos, Universidade Federal Rural do Rio de Janeiro, Serop?dica - RJ, 2014. |
url |
https://tede.ufrrj.br/jspui/handle/jspui/1235 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal Rural do Rio de Janeiro |
dc.publisher.program.fl_str_mv |
Programa de P?s-Gradua??o em Agronomia - Ci?ncia do Solo |
dc.publisher.initials.fl_str_mv |
UFRRJ |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Instituto de Agronomia |
publisher.none.fl_str_mv |
Universidade Federal Rural do Rio de Janeiro |
dc.source.none.fl_str_mv |
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