Potencial do latex da fruta pão (Artocarpus altilis) como agente coagulante do leite

Detalhes bibliográficos
Autor(a) principal: Soares,Elisângela França
Data de Publicação: 2015
Outros Autores: Silva,Anna Carolina da, Queiroz,Alana Emilia Soares de França, Gomes,José Erick Galindo, Herculano,Polyanna Nunes, Moreira,Keila Aparecida
Tipo de documento: Artigo
Idioma: por
Título da fonte: Ciência Rural
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015000100149
Resumo: Breadfruit is an exotic tree in Brazil, very well acclimatized. Despite of being a laticifer plant, the knowledge about its latex is scarce. However, it is known that proteolytic enzymes represents over 50% of the latex composition in laticifers plants. The aim of this study was to evaluate the potential of breadfruit (Artocarpus altilis var. Apyrena) latex as a source of milk clotting proteases and partially characterize it. The proteolytic activity of the fraction of crude extract was assessed using azocasein and quantitation of total protein, the bicinchoninic acid (BCA). Milk-clotting activity was analyzed using skim milk at 12%. The enzyme activity was analyzed under different temperature conditions (35 to 80°C) and pH (5.8 to 10.7) presenting optimum activity in alkaline pH (8.5) and 50°C; being stable to the two variables at 120 minutes during the test. The clotting activity was directly proportional to the temperature at the better concentration of CaCl2 (10mmol L-1). The results indicate that enzyme is a possible replacement for calf rennet
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spelling Potencial do latex da fruta pão (Artocarpus altilis) como agente coagulante do leiteenzimas coagulantes do leiteatividade proteolíticacoagulante vegetalfruta-pãoBreadfruit is an exotic tree in Brazil, very well acclimatized. Despite of being a laticifer plant, the knowledge about its latex is scarce. However, it is known that proteolytic enzymes represents over 50% of the latex composition in laticifers plants. The aim of this study was to evaluate the potential of breadfruit (Artocarpus altilis var. Apyrena) latex as a source of milk clotting proteases and partially characterize it. The proteolytic activity of the fraction of crude extract was assessed using azocasein and quantitation of total protein, the bicinchoninic acid (BCA). Milk-clotting activity was analyzed using skim milk at 12%. The enzyme activity was analyzed under different temperature conditions (35 to 80°C) and pH (5.8 to 10.7) presenting optimum activity in alkaline pH (8.5) and 50°C; being stable to the two variables at 120 minutes during the test. The clotting activity was directly proportional to the temperature at the better concentration of CaCl2 (10mmol L-1). The results indicate that enzyme is a possible replacement for calf rennetUniversidade Federal de Santa Maria2015-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015000100149Ciência Rural v.45 n.1 2015reponame:Ciência Ruralinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM10.1590/0103-8478cr20140310info:eu-repo/semantics/openAccessSoares,Elisângela FrançaSilva,Anna Carolina daQueiroz,Alana Emilia Soares de FrançaGomes,José Erick GalindoHerculano,Polyanna NunesMoreira,Keila Aparecidapor2016-05-12T00:00:00ZRevista
dc.title.none.fl_str_mv Potencial do latex da fruta pão (Artocarpus altilis) como agente coagulante do leite
title Potencial do latex da fruta pão (Artocarpus altilis) como agente coagulante do leite
spellingShingle Potencial do latex da fruta pão (Artocarpus altilis) como agente coagulante do leite
Soares,Elisângela França
enzimas coagulantes do leite
atividade proteolítica
coagulante vegetal
fruta-pão
title_short Potencial do latex da fruta pão (Artocarpus altilis) como agente coagulante do leite
title_full Potencial do latex da fruta pão (Artocarpus altilis) como agente coagulante do leite
title_fullStr Potencial do latex da fruta pão (Artocarpus altilis) como agente coagulante do leite
title_full_unstemmed Potencial do latex da fruta pão (Artocarpus altilis) como agente coagulante do leite
title_sort Potencial do latex da fruta pão (Artocarpus altilis) como agente coagulante do leite
author Soares,Elisângela França
author_facet Soares,Elisângela França
Silva,Anna Carolina da
Queiroz,Alana Emilia Soares de França
Gomes,José Erick Galindo
Herculano,Polyanna Nunes
Moreira,Keila Aparecida
author_role author
author2 Silva,Anna Carolina da
Queiroz,Alana Emilia Soares de França
Gomes,José Erick Galindo
Herculano,Polyanna Nunes
Moreira,Keila Aparecida
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Soares,Elisângela França
Silva,Anna Carolina da
Queiroz,Alana Emilia Soares de França
Gomes,José Erick Galindo
Herculano,Polyanna Nunes
Moreira,Keila Aparecida
dc.subject.por.fl_str_mv enzimas coagulantes do leite
atividade proteolítica
coagulante vegetal
fruta-pão
topic enzimas coagulantes do leite
atividade proteolítica
coagulante vegetal
fruta-pão
description Breadfruit is an exotic tree in Brazil, very well acclimatized. Despite of being a laticifer plant, the knowledge about its latex is scarce. However, it is known that proteolytic enzymes represents over 50% of the latex composition in laticifers plants. The aim of this study was to evaluate the potential of breadfruit (Artocarpus altilis var. Apyrena) latex as a source of milk clotting proteases and partially characterize it. The proteolytic activity of the fraction of crude extract was assessed using azocasein and quantitation of total protein, the bicinchoninic acid (BCA). Milk-clotting activity was analyzed using skim milk at 12%. The enzyme activity was analyzed under different temperature conditions (35 to 80°C) and pH (5.8 to 10.7) presenting optimum activity in alkaline pH (8.5) and 50°C; being stable to the two variables at 120 minutes during the test. The clotting activity was directly proportional to the temperature at the better concentration of CaCl2 (10mmol L-1). The results indicate that enzyme is a possible replacement for calf rennet
publishDate 2015
dc.date.none.fl_str_mv 2015-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015000100149
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015000100149
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv 10.1590/0103-8478cr20140310
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Universidade Federal de Santa Maria
publisher.none.fl_str_mv Universidade Federal de Santa Maria
dc.source.none.fl_str_mv Ciência Rural v.45 n.1 2015
reponame:Ciência Rural
instname:Universidade Federal de Santa Maria (UFSM)
instacron:UFSM
instname_str Universidade Federal de Santa Maria (UFSM)
instacron_str UFSM
institution UFSM
reponame_str Ciência Rural
collection Ciência Rural
repository.name.fl_str_mv
repository.mail.fl_str_mv
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