Antibody responses in mice after immunization with inactivated bovine respiratory syncytial virus using different adjuvants
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Ciência Rural |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782010001100013 |
Resumo: | Bovine respiratory syncytial virus (BRSV) causes pneumonia in young cattle. Modified-live-virus (MLV) and inactivated vaccines are currently used for the control of clinical effects of BRSV infections in cattle. On the present research, the stimulation of specific anti-BRSV immunoglobulin isotypes was investigated, through the use of different commercially available adjuvants (Water-in-oil emulsion, Quil A, Aluminum-hydroxide) in inbred mice (Balb/C and C57BL/6). BRSV antibodies were measured using an enzyme-linked immunosorbent assay (ELISA) and the results were compared to the antibody levels induced by immunization of animals using live-BRSV-virus. Water-in-oil emulsion and alum- adjuvant preparations induced higher levels of IgG1 immunoglobulins, whereas Quil A favored the production of IgG2 antibodies, this last being a more appropriate response profile for the specific case of BRSV. Not using adjuvants resulted in poor levels of specific antibodies. The isotype profile of specific antibodies obtained varied greatly depending on the adjuvants used. This information may be useful for the formulation of more effective BRSV inactivated vaccines; however, these findings have to be confirmed in cattle. |
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Antibody responses in mice after immunization with inactivated bovine respiratory syncytial virus using different adjuvantsbovine respiratory syncytial virusBRSVadjuvantsmurine modelinactivated vaccinesBovine respiratory syncytial virus (BRSV) causes pneumonia in young cattle. Modified-live-virus (MLV) and inactivated vaccines are currently used for the control of clinical effects of BRSV infections in cattle. On the present research, the stimulation of specific anti-BRSV immunoglobulin isotypes was investigated, through the use of different commercially available adjuvants (Water-in-oil emulsion, Quil A, Aluminum-hydroxide) in inbred mice (Balb/C and C57BL/6). BRSV antibodies were measured using an enzyme-linked immunosorbent assay (ELISA) and the results were compared to the antibody levels induced by immunization of animals using live-BRSV-virus. Water-in-oil emulsion and alum- adjuvant preparations induced higher levels of IgG1 immunoglobulins, whereas Quil A favored the production of IgG2 antibodies, this last being a more appropriate response profile for the specific case of BRSV. Not using adjuvants resulted in poor levels of specific antibodies. The isotype profile of specific antibodies obtained varied greatly depending on the adjuvants used. This information may be useful for the formulation of more effective BRSV inactivated vaccines; however, these findings have to be confirmed in cattle.Universidade Federal de Santa Maria2010-11-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782010001100013Ciência Rural v.40 n.11 2010reponame:Ciência Ruralinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM10.1590/S0103-84782010005000188info:eu-repo/semantics/openAccessSpilki,Fernando RosadoAlmeida,Renata Servan deArns,Clarice Weiseng2011-03-21T00:00:00ZRevista |
dc.title.none.fl_str_mv |
Antibody responses in mice after immunization with inactivated bovine respiratory syncytial virus using different adjuvants |
title |
Antibody responses in mice after immunization with inactivated bovine respiratory syncytial virus using different adjuvants |
spellingShingle |
Antibody responses in mice after immunization with inactivated bovine respiratory syncytial virus using different adjuvants Spilki,Fernando Rosado bovine respiratory syncytial virus BRSV adjuvants murine model inactivated vaccines |
title_short |
Antibody responses in mice after immunization with inactivated bovine respiratory syncytial virus using different adjuvants |
title_full |
Antibody responses in mice after immunization with inactivated bovine respiratory syncytial virus using different adjuvants |
title_fullStr |
Antibody responses in mice after immunization with inactivated bovine respiratory syncytial virus using different adjuvants |
title_full_unstemmed |
Antibody responses in mice after immunization with inactivated bovine respiratory syncytial virus using different adjuvants |
title_sort |
Antibody responses in mice after immunization with inactivated bovine respiratory syncytial virus using different adjuvants |
author |
Spilki,Fernando Rosado |
author_facet |
Spilki,Fernando Rosado Almeida,Renata Servan de Arns,Clarice Weis |
author_role |
author |
author2 |
Almeida,Renata Servan de Arns,Clarice Weis |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Spilki,Fernando Rosado Almeida,Renata Servan de Arns,Clarice Weis |
dc.subject.por.fl_str_mv |
bovine respiratory syncytial virus BRSV adjuvants murine model inactivated vaccines |
topic |
bovine respiratory syncytial virus BRSV adjuvants murine model inactivated vaccines |
description |
Bovine respiratory syncytial virus (BRSV) causes pneumonia in young cattle. Modified-live-virus (MLV) and inactivated vaccines are currently used for the control of clinical effects of BRSV infections in cattle. On the present research, the stimulation of specific anti-BRSV immunoglobulin isotypes was investigated, through the use of different commercially available adjuvants (Water-in-oil emulsion, Quil A, Aluminum-hydroxide) in inbred mice (Balb/C and C57BL/6). BRSV antibodies were measured using an enzyme-linked immunosorbent assay (ELISA) and the results were compared to the antibody levels induced by immunization of animals using live-BRSV-virus. Water-in-oil emulsion and alum- adjuvant preparations induced higher levels of IgG1 immunoglobulins, whereas Quil A favored the production of IgG2 antibodies, this last being a more appropriate response profile for the specific case of BRSV. Not using adjuvants resulted in poor levels of specific antibodies. The isotype profile of specific antibodies obtained varied greatly depending on the adjuvants used. This information may be useful for the formulation of more effective BRSV inactivated vaccines; however, these findings have to be confirmed in cattle. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-11-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782010001100013 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782010001100013 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0103-84782010005000188 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria |
dc.source.none.fl_str_mv |
Ciência Rural v.40 n.11 2010 reponame:Ciência Rural instname:Universidade Federal de Santa Maria (UFSM) instacron:UFSM |
instname_str |
Universidade Federal de Santa Maria (UFSM) |
instacron_str |
UFSM |
institution |
UFSM |
reponame_str |
Ciência Rural |
collection |
Ciência Rural |
repository.name.fl_str_mv |
|
repository.mail.fl_str_mv |
|
_version_ |
1749140537838927872 |