Genômica de um baculovírus isolado da lagarta praga de leguminosas Urbanus proteus (Linnaeus, 1758) (Lepidoptera: Hesperiidae)
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações do UFSM |
Texto Completo: | http://repositorio.ufsm.br/handle/1/16276 |
Resumo: | Baculoviruses are insect viruses largely used as vectors for gene expression and biopesticides. These viruses can efficiently infect larvae of several agricultural pests worldwide, causing a lethal disease. In this work, a novel baculovirus isolated from the larval stage of Urbanus proteus (L.), the bean leafhopper caterpillar was found in the 1980s and the complete genome was characterized. This was an important pest of several legumes in Brazil and belongs to the butterfly family Hesperiidae, from where no baculovirus genome sequence has been described. This new virus was shown to have the smallest genome among all alphabaculoviruses sequenced to date, with 105,555 bp and 119 putative ORFs. Ten unique genes, seven bro genes and the 38 baculovirus core genes were found. UrprNPV was found to be related to the Adoxophyes-infecting baculoviruses AdorNPV and AdhoNPV, with high genetic distance and long branch length. Interestingly, few individual core gene-based phylogenies were found to support the relationship of UrprNPV to both AdorNPV and AdhoNPV. Importantly to note that the increase in the number of completely sequenced baculoviruses points to an interesting way of understanding baculoviruses and their evolution, potentially helping the use of baculovirus, both as biopesticide and expression vectors. |
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2019-04-22T13:41:20Z2019-04-22T13:41:20Z2018-07-17http://repositorio.ufsm.br/handle/1/16276Baculoviruses are insect viruses largely used as vectors for gene expression and biopesticides. These viruses can efficiently infect larvae of several agricultural pests worldwide, causing a lethal disease. In this work, a novel baculovirus isolated from the larval stage of Urbanus proteus (L.), the bean leafhopper caterpillar was found in the 1980s and the complete genome was characterized. This was an important pest of several legumes in Brazil and belongs to the butterfly family Hesperiidae, from where no baculovirus genome sequence has been described. This new virus was shown to have the smallest genome among all alphabaculoviruses sequenced to date, with 105,555 bp and 119 putative ORFs. Ten unique genes, seven bro genes and the 38 baculovirus core genes were found. UrprNPV was found to be related to the Adoxophyes-infecting baculoviruses AdorNPV and AdhoNPV, with high genetic distance and long branch length. Interestingly, few individual core gene-based phylogenies were found to support the relationship of UrprNPV to both AdorNPV and AdhoNPV. Importantly to note that the increase in the number of completely sequenced baculoviruses points to an interesting way of understanding baculoviruses and their evolution, potentially helping the use of baculovirus, both as biopesticide and expression vectors.Os baculovírus são vírus de insetos amplamente utilizados como vetores de expressão gênica e biopesticidas. Esses vírus podem infectar eficientemente larvas de várias pragas agrícolas em todo o mundo, causando uma doença letal. Neste trabalho, foi encontrado um novo baculovírus isolado do estágio larval de Urbanus proteus (L.), a lagarta desfolhadora do feijão nos anos de 1980 e foi caracterizado o seu genoma completo. Esta foi uma importante praga de várias leguminosas no Brasil e pertence à família das borboletas Hesperiidae, de onde não foi descrita nenhuma sequência genômica de baculovírus. Este novo vírus mostrou ter o menor genoma entre todos os alphabaculovírus sequenciados até o momento, com 105.555 pb e 119 ORFs. Foram encontrados dez genes únicos, sete genes bro e os 38 genes compartilhados entre todos os baculovírus. Verificou-se que UrprNPV estava relacionado com os baculovírus que infectam o gênero Adoxophyes, AdorNPV e AdhoNPV, com alta distância genética e comprimento longo do ramo. Curiosamente, poucas filogenias baseadas nos genes compartilhados individuais apoiaram a relação de UrprNPV com ambos, AdorNPV e AdhoNPV. É importante ressaltar que o aumento no número de baculovírus completamente sequenciados aponta para uma forma interessante de entender os baculovírus e a sua evolução, podendo ajudar potencialmente o uso de baculovírus tanto como biopesticida e vetores de expressão.Conselho Nacional de Pesquisa e Desenvolvimento Científico e Tecnológico - CNPqporUniversidade Federal de Santa MariaCentro de Ciências Naturais e ExatasPrograma de Pós-Graduação em Ciências Biológicas: Bioquímica ToxicológicaUFSMBrasilBioquímicaAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessGenômicaBaculovírusAlphabaculovirusUrbanus proteusEvoluçãoGenomicEvolutionCNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICAGenômica de um baculovírus isolado da lagarta praga de leguminosas Urbanus proteus (Linnaeus, 1758) (Lepidoptera: Hesperiidae)Genomics of a baculovirus isolated from a leguminous pest, Urbanus proteus (Linnaeus, 1758) (Lepidoptera: Hesperiidae)info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisAraújo, Daniel Mendes Pereira Ardisson dehttp://lattes.cnpq.br/5900778605189135Ribeiro, Bergmann Moraishttp://lattes.cnpq.br/5505277659734432Flores, Eduardo Furtadohttp://lattes.cnpq.br/0446078331070694http://lattes.cnpq.br/5341201859493503Santos, Ethiane Rozo dos200800000002600aabc4145-c1d0-44eb-9b5f-1eaf18da628614bdedd9-d10e-4b7a-8112-322058df67ea0e791dfb-9fc6-4917-a9c6-d93574b2220324a70626-00df-46f5-b9a4-eb83847574bfreponame:Biblioteca Digital de Teses e Dissertações do UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMLICENSElicense.txtlicense.txttext/plain; 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dc.title.por.fl_str_mv |
Genômica de um baculovírus isolado da lagarta praga de leguminosas Urbanus proteus (Linnaeus, 1758) (Lepidoptera: Hesperiidae) |
dc.title.alternative.eng.fl_str_mv |
Genomics of a baculovirus isolated from a leguminous pest, Urbanus proteus (Linnaeus, 1758) (Lepidoptera: Hesperiidae) |
title |
Genômica de um baculovírus isolado da lagarta praga de leguminosas Urbanus proteus (Linnaeus, 1758) (Lepidoptera: Hesperiidae) |
spellingShingle |
Genômica de um baculovírus isolado da lagarta praga de leguminosas Urbanus proteus (Linnaeus, 1758) (Lepidoptera: Hesperiidae) Santos, Ethiane Rozo dos Genômica Baculovírus Alphabaculovirus Urbanus proteus Evolução Genomic Evolution CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
title_short |
Genômica de um baculovírus isolado da lagarta praga de leguminosas Urbanus proteus (Linnaeus, 1758) (Lepidoptera: Hesperiidae) |
title_full |
Genômica de um baculovírus isolado da lagarta praga de leguminosas Urbanus proteus (Linnaeus, 1758) (Lepidoptera: Hesperiidae) |
title_fullStr |
Genômica de um baculovírus isolado da lagarta praga de leguminosas Urbanus proteus (Linnaeus, 1758) (Lepidoptera: Hesperiidae) |
title_full_unstemmed |
Genômica de um baculovírus isolado da lagarta praga de leguminosas Urbanus proteus (Linnaeus, 1758) (Lepidoptera: Hesperiidae) |
title_sort |
Genômica de um baculovírus isolado da lagarta praga de leguminosas Urbanus proteus (Linnaeus, 1758) (Lepidoptera: Hesperiidae) |
author |
Santos, Ethiane Rozo dos |
author_facet |
Santos, Ethiane Rozo dos |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Araújo, Daniel Mendes Pereira Ardisson de |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/5900778605189135 |
dc.contributor.referee1.fl_str_mv |
Ribeiro, Bergmann Morais |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/5505277659734432 |
dc.contributor.referee2.fl_str_mv |
Flores, Eduardo Furtado |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/0446078331070694 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/5341201859493503 |
dc.contributor.author.fl_str_mv |
Santos, Ethiane Rozo dos |
contributor_str_mv |
Araújo, Daniel Mendes Pereira Ardisson de Ribeiro, Bergmann Morais Flores, Eduardo Furtado |
dc.subject.por.fl_str_mv |
Genômica Baculovírus Alphabaculovirus Urbanus proteus Evolução |
topic |
Genômica Baculovírus Alphabaculovirus Urbanus proteus Evolução Genomic Evolution CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
dc.subject.eng.fl_str_mv |
Genomic Evolution |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
description |
Baculoviruses are insect viruses largely used as vectors for gene expression and biopesticides. These viruses can efficiently infect larvae of several agricultural pests worldwide, causing a lethal disease. In this work, a novel baculovirus isolated from the larval stage of Urbanus proteus (L.), the bean leafhopper caterpillar was found in the 1980s and the complete genome was characterized. This was an important pest of several legumes in Brazil and belongs to the butterfly family Hesperiidae, from where no baculovirus genome sequence has been described. This new virus was shown to have the smallest genome among all alphabaculoviruses sequenced to date, with 105,555 bp and 119 putative ORFs. Ten unique genes, seven bro genes and the 38 baculovirus core genes were found. UrprNPV was found to be related to the Adoxophyes-infecting baculoviruses AdorNPV and AdhoNPV, with high genetic distance and long branch length. Interestingly, few individual core gene-based phylogenies were found to support the relationship of UrprNPV to both AdorNPV and AdhoNPV. Importantly to note that the increase in the number of completely sequenced baculoviruses points to an interesting way of understanding baculoviruses and their evolution, potentially helping the use of baculovirus, both as biopesticide and expression vectors. |
publishDate |
2018 |
dc.date.issued.fl_str_mv |
2018-07-17 |
dc.date.accessioned.fl_str_mv |
2019-04-22T13:41:20Z |
dc.date.available.fl_str_mv |
2019-04-22T13:41:20Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
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masterThesis |
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publishedVersion |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufsm.br/handle/1/16276 |
url |
http://repositorio.ufsm.br/handle/1/16276 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.cnpq.fl_str_mv |
200800000002 |
dc.relation.confidence.fl_str_mv |
600 |
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dc.rights.driver.fl_str_mv |
Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Centro de Ciências Naturais e Exatas |
dc.publisher.program.fl_str_mv |
Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica |
dc.publisher.initials.fl_str_mv |
UFSM |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Bioquímica |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Centro de Ciências Naturais e Exatas |
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