Hidrólise extracelular de ATP e ADP pela enzima NTPDase1 em linfócitos de pacientes com leucemia linfocítica crônica-B
Autor(a) principal: | |
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Data de Publicação: | 2006 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações do UFSM |
Texto Completo: | http://repositorio.ufsm.br/handle/1/11149 |
Resumo: | The NTPDase1 enzymatic activity (E.C.3.6.1.5, Apyrase, ecto-ATP-diphosphohydrolase, CD39) which hydrolyses ATP and ADP, was analyzed in peripheral lymphocytes from patients with B chronic lymphocytic leukemia (B-CLL) which the main characteristic is the accumulation of monoclonal mature B-lymphocyte. The sample was composed by 23 patients with B-CLL whose were diagnosed according to the clinical and laboratorial criteria and also by the immunophenotypic characteristics. The patients were divided, according to the Binet staging system, in 4 groups: stage A (early), stage B (intermediate), stage C (advanced) and control group. The results demonstrated a significant increase in ATP hydrolysis (F(29,3)=26.79 p<0.001) for all the stages of the disease and related to the control as a function of length of disease advance with higher activity in stage C. ADP hydrolysis (F(29,3)=23.76 p<0.001) was also enhanced according to ATP results. Besides this it was found a great correlation between the activity of NTPDase-1 and the total absolute white cells count in peripheral blood. The alteration in NTPDase-1activity in lymphocytes from patients with B-CLL at the different stages, contributes to confirm the ectonucleotidases role in regulate the ATP and ADP levels, which actuates like signalling molecules in many blood cells like the lymphocytes. Therefore more studies need to be done so that we can elucidate such enzymatic system in the nucleotides and its role in the B-CLL. |
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2017-04-262017-04-262006-02-23ZANIN, Rafael Fernandes. Extracellular ATP and ADP hydrolyzed by the NTPDase1 enzyme in lymphocytes from B-chronic lymphocytic leukemia patients. 2006. 73 f. Dissertação (Mestrado em Bioquímica) - Universidade Federal de Santa Maria, Santa Maria, 2006.http://repositorio.ufsm.br/handle/1/11149The NTPDase1 enzymatic activity (E.C.3.6.1.5, Apyrase, ecto-ATP-diphosphohydrolase, CD39) which hydrolyses ATP and ADP, was analyzed in peripheral lymphocytes from patients with B chronic lymphocytic leukemia (B-CLL) which the main characteristic is the accumulation of monoclonal mature B-lymphocyte. The sample was composed by 23 patients with B-CLL whose were diagnosed according to the clinical and laboratorial criteria and also by the immunophenotypic characteristics. The patients were divided, according to the Binet staging system, in 4 groups: stage A (early), stage B (intermediate), stage C (advanced) and control group. The results demonstrated a significant increase in ATP hydrolysis (F(29,3)=26.79 p<0.001) for all the stages of the disease and related to the control as a function of length of disease advance with higher activity in stage C. ADP hydrolysis (F(29,3)=23.76 p<0.001) was also enhanced according to ATP results. Besides this it was found a great correlation between the activity of NTPDase-1 and the total absolute white cells count in peripheral blood. The alteration in NTPDase-1activity in lymphocytes from patients with B-CLL at the different stages, contributes to confirm the ectonucleotidases role in regulate the ATP and ADP levels, which actuates like signalling molecules in many blood cells like the lymphocytes. Therefore more studies need to be done so that we can elucidate such enzymatic system in the nucleotides and its role in the B-CLL.A atividade da enzima NTPDase1 (E.C.3.6.1.5, Apyrase, ecto-ATP-difosfoidrolase,CD39) que hidrolisa ATP e ADP, foi analisada em linfócitos periféricos de pacientes com leucemia linfocítica crônica (LLC-B), doença que possui como característica principal o acúmulo de linfócitos B maduros. A amostra foi composta por 23 pacientes com LLC-B, diagnosticados de acordo com critérios clínicos, laboratoriais e resultados de imunofenotipagem. Os pacientes foram divididos, conforme o sistema de classificação de Binet, em 4 grupos: estágio A (inicial), estágio B (intermediário), estágio C (avançado) e um grupo controle. Os resultados demonstraram que a hidrólise do ATP foi significativamente aumentada (F(29,3)=26.79 p<0.001) em todos os estágios da doença e em relação ao controle e esse aumento é compatível com o avanço da doença mostrando a maior hidrólise no estágio C. A hidrólise do ADP acompanhou os resultados do ATP e também estava aumentada (F(29,3)= 23.76 p<0.001). Além disso, verificou-se uma forte correlação entre a atividade da enzima NTPDase1 e a contagem absoluta de glóbulos brancos do sangue periférico. A alteração na atividade da enzima NTPDase1 em linfócitos de pacientes com LLC-B em seus diferentes estágios, vem confirmar o importante papel das ecto-nucleotidases na regulação dos níveis de ATP e ADP, os quais atuam como moléculas sinalizadores em várias células sangüíneas como os linfócitos. Entretanto, mais estudos são necessários para melhor elucidar as funções desse sistema enzimático no controle de nucleotídeos e o seu papel na LLC-B.application/pdfporUniversidade Federal de Santa MariaPrograma de Pós-Graduação em Ciências Biológicas: Bioquímica ToxicológicaUFSMBRBioquímicaATPADPNTPDase1LinfócitosLLC-BLymphocytesCNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICAHidrólise extracelular de ATP e ADP pela enzima NTPDase1 em linfócitos de pacientes com leucemia linfocítica crônica-BExtracellular ATP and ADP hydrolyzed by the NTPDase1 enzyme in lymphocytes from B-chronic lymphocytic leukemia patientsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisSchetinger, Maria Rosa ChitolinaGraça, Dominguita Luhershttp://lattes.cnpq.br/7761878749916936Soares, Félix Alexandre Antuneshttp://lattes.cnpq.br/8752453650114092http://lattes.cnpq.br/7751771425381246Zanin, Rafael Fernandes200800000002400500300500500f2c5f39c-089e-4825-bfba-cc66b9098981d04efd90-bd9c-4c96-92c6-3d135ef59b375b1f94f9-23f7-49df-a620-c35a152c9039161311d0-e9c6-4e08-a436-85b4822199b4info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações do UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMORIGINALRAFAEL.pdfapplication/pdf350067http://repositorio.ufsm.br/bitstream/1/11149/1/RAFAEL.pdff70d4025cd2dff624dc74ec8af0c91a0MD511/111492022-08-25 16:41:06.044oai:repositorio.ufsm.br:1/11149Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufsm.br/ONGhttps://repositorio.ufsm.br/oai/requestatendimento.sib@ufsm.br||tedebc@gmail.comopendoar:2022-08-25T19:41:06Biblioteca Digital de Teses e Dissertações do UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.por.fl_str_mv |
Hidrólise extracelular de ATP e ADP pela enzima NTPDase1 em linfócitos de pacientes com leucemia linfocítica crônica-B |
dc.title.alternative.eng.fl_str_mv |
Extracellular ATP and ADP hydrolyzed by the NTPDase1 enzyme in lymphocytes from B-chronic lymphocytic leukemia patients |
title |
Hidrólise extracelular de ATP e ADP pela enzima NTPDase1 em linfócitos de pacientes com leucemia linfocítica crônica-B |
spellingShingle |
Hidrólise extracelular de ATP e ADP pela enzima NTPDase1 em linfócitos de pacientes com leucemia linfocítica crônica-B Zanin, Rafael Fernandes ATP ADP NTPDase1 Linfócitos LLC-B Lymphocytes CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
title_short |
Hidrólise extracelular de ATP e ADP pela enzima NTPDase1 em linfócitos de pacientes com leucemia linfocítica crônica-B |
title_full |
Hidrólise extracelular de ATP e ADP pela enzima NTPDase1 em linfócitos de pacientes com leucemia linfocítica crônica-B |
title_fullStr |
Hidrólise extracelular de ATP e ADP pela enzima NTPDase1 em linfócitos de pacientes com leucemia linfocítica crônica-B |
title_full_unstemmed |
Hidrólise extracelular de ATP e ADP pela enzima NTPDase1 em linfócitos de pacientes com leucemia linfocítica crônica-B |
title_sort |
Hidrólise extracelular de ATP e ADP pela enzima NTPDase1 em linfócitos de pacientes com leucemia linfocítica crônica-B |
author |
Zanin, Rafael Fernandes |
author_facet |
Zanin, Rafael Fernandes |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Schetinger, Maria Rosa Chitolina |
dc.contributor.referee1.fl_str_mv |
Graça, Dominguita Luhers |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/7761878749916936 |
dc.contributor.referee2.fl_str_mv |
Soares, Félix Alexandre Antunes |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/8752453650114092 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/7751771425381246 |
dc.contributor.author.fl_str_mv |
Zanin, Rafael Fernandes |
contributor_str_mv |
Schetinger, Maria Rosa Chitolina Graça, Dominguita Luhers Soares, Félix Alexandre Antunes |
dc.subject.por.fl_str_mv |
ATP ADP NTPDase1 Linfócitos LLC-B |
topic |
ATP ADP NTPDase1 Linfócitos LLC-B Lymphocytes CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
dc.subject.eng.fl_str_mv |
Lymphocytes |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
description |
The NTPDase1 enzymatic activity (E.C.3.6.1.5, Apyrase, ecto-ATP-diphosphohydrolase, CD39) which hydrolyses ATP and ADP, was analyzed in peripheral lymphocytes from patients with B chronic lymphocytic leukemia (B-CLL) which the main characteristic is the accumulation of monoclonal mature B-lymphocyte. The sample was composed by 23 patients with B-CLL whose were diagnosed according to the clinical and laboratorial criteria and also by the immunophenotypic characteristics. The patients were divided, according to the Binet staging system, in 4 groups: stage A (early), stage B (intermediate), stage C (advanced) and control group. The results demonstrated a significant increase in ATP hydrolysis (F(29,3)=26.79 p<0.001) for all the stages of the disease and related to the control as a function of length of disease advance with higher activity in stage C. ADP hydrolysis (F(29,3)=23.76 p<0.001) was also enhanced according to ATP results. Besides this it was found a great correlation between the activity of NTPDase-1 and the total absolute white cells count in peripheral blood. The alteration in NTPDase-1activity in lymphocytes from patients with B-CLL at the different stages, contributes to confirm the ectonucleotidases role in regulate the ATP and ADP levels, which actuates like signalling molecules in many blood cells like the lymphocytes. Therefore more studies need to be done so that we can elucidate such enzymatic system in the nucleotides and its role in the B-CLL. |
publishDate |
2006 |
dc.date.issued.fl_str_mv |
2006-02-23 |
dc.date.accessioned.fl_str_mv |
2017-04-26 |
dc.date.available.fl_str_mv |
2017-04-26 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
ZANIN, Rafael Fernandes. Extracellular ATP and ADP hydrolyzed by the NTPDase1 enzyme in lymphocytes from B-chronic lymphocytic leukemia patients. 2006. 73 f. Dissertação (Mestrado em Bioquímica) - Universidade Federal de Santa Maria, Santa Maria, 2006. |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufsm.br/handle/1/11149 |
identifier_str_mv |
ZANIN, Rafael Fernandes. Extracellular ATP and ADP hydrolyzed by the NTPDase1 enzyme in lymphocytes from B-chronic lymphocytic leukemia patients. 2006. 73 f. Dissertação (Mestrado em Bioquímica) - Universidade Federal de Santa Maria, Santa Maria, 2006. |
url |
http://repositorio.ufsm.br/handle/1/11149 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.cnpq.fl_str_mv |
200800000002 |
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400 500 300 500 500 |
dc.relation.authority.fl_str_mv |
f2c5f39c-089e-4825-bfba-cc66b9098981 d04efd90-bd9c-4c96-92c6-3d135ef59b37 5b1f94f9-23f7-49df-a620-c35a152c9039 161311d0-e9c6-4e08-a436-85b4822199b4 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria |
dc.publisher.program.fl_str_mv |
Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica |
dc.publisher.initials.fl_str_mv |
UFSM |
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BR |
dc.publisher.department.fl_str_mv |
Bioquímica |
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Universidade Federal de Santa Maria |
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Biblioteca Digital de Teses e Dissertações do UFSM |
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Biblioteca Digital de Teses e Dissertações do UFSM |
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Biblioteca Digital de Teses e Dissertações do UFSM - Universidade Federal de Santa Maria (UFSM) |
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