Culture supernatant of Shiga toxin-producing Escherichia coli strains provoke fluid accumulation in rabbit ileal loops

Detalhes bibliográficos
Autor(a) principal: Ferreira, Antonio José Piantino
Data de Publicação: 1997
Outros Autores: Elias Junior, Waldir Pereira [UNIFESP], Pelayo, Jacinta Sanchez, Giraldi, Regina [UNIFESP], Pedroso, Margareth Zabeu [UNIFESP], Scaletsky, Isabel Cristina Affonso [UNIFESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://repositorio.unifesp.br/handle/11600/25814
http://dx.doi.org/10.1111/j.1574-695X.1997.tb01098.x
Resumo: Production of Shiga toxin (Stx) in Escherichia coli strains belonging to serogroups O26, O111, and O157 was evaluated in the rabbit ileal loop assay and results were compared to those using tissue culture assays and DNA hybridization with specific probes for Stx1 and Stx2. All 14 Shiga toxin-producing E. coli strains tested provoked fluid accumulation in the rabbit intestinal loop. Eleven strains hybridized with Stx1 probe, one strain with Stx2 and two strains with both probes. Filtered culture supernatants of all E. coli strains presented cytotoxic effects in both HeLa and Vero cells, in this study, we found a strong association between the production of Stx and its effect in an animal model. This is the first description of high-level Stx-producing E. coli O111ac isolated in Brazil. (C) 1998 Published by Elsevier Science B.V. All rights reserved.
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spelling Ferreira, Antonio José PiantinoElias Junior, Waldir Pereira [UNIFESP]Pelayo, Jacinta SanchezGiraldi, Regina [UNIFESP]Pedroso, Margareth Zabeu [UNIFESP]Scaletsky, Isabel Cristina Affonso [UNIFESP]Universidade Federal de São Paulo (UNIFESP)Universidade de São Paulo (USP)Universidade Estadual de Londrina (UEL)2016-01-24T12:30:29Z2016-01-24T12:30:29Z1997-12-01Fems Immunology and Medical Microbiology. Hoboken: Wiley-Blackwell, v. 19, n. 4, p. 285-288, 1997.0928-8244http://repositorio.unifesp.br/handle/11600/25814http://dx.doi.org/10.1111/j.1574-695X.1997.tb01098.x10.1111/j.1574-695X.1997.tb01098.xWOS:000071594600004Production of Shiga toxin (Stx) in Escherichia coli strains belonging to serogroups O26, O111, and O157 was evaluated in the rabbit ileal loop assay and results were compared to those using tissue culture assays and DNA hybridization with specific probes for Stx1 and Stx2. All 14 Shiga toxin-producing E. coli strains tested provoked fluid accumulation in the rabbit intestinal loop. Eleven strains hybridized with Stx1 probe, one strain with Stx2 and two strains with both probes. Filtered culture supernatants of all E. coli strains presented cytotoxic effects in both HeLa and Vero cells, in this study, we found a strong association between the production of Stx and its effect in an animal model. This is the first description of high-level Stx-producing E. coli O111ac isolated in Brazil. (C) 1998 Published by Elsevier Science B.V. All rights reserved.Universidade Federal de São Paulo, Dept Microbiol Immunol & Parasitol, BR-04023062 São Paulo, BrazilUniv São Paulo, Fac Med Vet & Zootecn, Dept Patol, São Paulo, BrazilUniv Estadual Londrina, Dept Patol Geral, Londrina, BrazilUniversidade Federal de São Paulo, Dept Microbiol Immunol & Parasitol, BR-04023062 São Paulo, BrazilWeb of Science285-288engWiley-BlackwellFems Immunology and Medical Microbiologyhttp://olabout.wiley.com/WileyCDA/Section/id-406071.htmlinfo:eu-repo/semantics/openAccessEscherichia colishiga-like toxinrabbit ileal loop assayCulture supernatant of Shiga toxin-producing Escherichia coli strains provoke fluid accumulation in rabbit ileal loopsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlereponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP11600/258142022-09-27 09:48:38.381metadata only accessoai:repositorio.unifesp.br:11600/25814Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652023-05-25T12:23:46.271899Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.en.fl_str_mv Culture supernatant of Shiga toxin-producing Escherichia coli strains provoke fluid accumulation in rabbit ileal loops
title Culture supernatant of Shiga toxin-producing Escherichia coli strains provoke fluid accumulation in rabbit ileal loops
spellingShingle Culture supernatant of Shiga toxin-producing Escherichia coli strains provoke fluid accumulation in rabbit ileal loops
Ferreira, Antonio José Piantino
Escherichia coli
shiga-like toxin
rabbit ileal loop assay
title_short Culture supernatant of Shiga toxin-producing Escherichia coli strains provoke fluid accumulation in rabbit ileal loops
title_full Culture supernatant of Shiga toxin-producing Escherichia coli strains provoke fluid accumulation in rabbit ileal loops
title_fullStr Culture supernatant of Shiga toxin-producing Escherichia coli strains provoke fluid accumulation in rabbit ileal loops
title_full_unstemmed Culture supernatant of Shiga toxin-producing Escherichia coli strains provoke fluid accumulation in rabbit ileal loops
title_sort Culture supernatant of Shiga toxin-producing Escherichia coli strains provoke fluid accumulation in rabbit ileal loops
author Ferreira, Antonio José Piantino
author_facet Ferreira, Antonio José Piantino
Elias Junior, Waldir Pereira [UNIFESP]
Pelayo, Jacinta Sanchez
Giraldi, Regina [UNIFESP]
Pedroso, Margareth Zabeu [UNIFESP]
Scaletsky, Isabel Cristina Affonso [UNIFESP]
author_role author
author2 Elias Junior, Waldir Pereira [UNIFESP]
Pelayo, Jacinta Sanchez
Giraldi, Regina [UNIFESP]
Pedroso, Margareth Zabeu [UNIFESP]
Scaletsky, Isabel Cristina Affonso [UNIFESP]
author2_role author
author
author
author
author
dc.contributor.institution.none.fl_str_mv Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
Universidade Estadual de Londrina (UEL)
dc.contributor.author.fl_str_mv Ferreira, Antonio José Piantino
Elias Junior, Waldir Pereira [UNIFESP]
Pelayo, Jacinta Sanchez
Giraldi, Regina [UNIFESP]
Pedroso, Margareth Zabeu [UNIFESP]
Scaletsky, Isabel Cristina Affonso [UNIFESP]
dc.subject.eng.fl_str_mv Escherichia coli
shiga-like toxin
rabbit ileal loop assay
topic Escherichia coli
shiga-like toxin
rabbit ileal loop assay
description Production of Shiga toxin (Stx) in Escherichia coli strains belonging to serogroups O26, O111, and O157 was evaluated in the rabbit ileal loop assay and results were compared to those using tissue culture assays and DNA hybridization with specific probes for Stx1 and Stx2. All 14 Shiga toxin-producing E. coli strains tested provoked fluid accumulation in the rabbit intestinal loop. Eleven strains hybridized with Stx1 probe, one strain with Stx2 and two strains with both probes. Filtered culture supernatants of all E. coli strains presented cytotoxic effects in both HeLa and Vero cells, in this study, we found a strong association between the production of Stx and its effect in an animal model. This is the first description of high-level Stx-producing E. coli O111ac isolated in Brazil. (C) 1998 Published by Elsevier Science B.V. All rights reserved.
publishDate 1997
dc.date.issued.fl_str_mv 1997-12-01
dc.date.accessioned.fl_str_mv 2016-01-24T12:30:29Z
dc.date.available.fl_str_mv 2016-01-24T12:30:29Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv Fems Immunology and Medical Microbiology. Hoboken: Wiley-Blackwell, v. 19, n. 4, p. 285-288, 1997.
dc.identifier.uri.fl_str_mv http://repositorio.unifesp.br/handle/11600/25814
http://dx.doi.org/10.1111/j.1574-695X.1997.tb01098.x
dc.identifier.issn.none.fl_str_mv 0928-8244
dc.identifier.doi.none.fl_str_mv 10.1111/j.1574-695X.1997.tb01098.x
dc.identifier.wos.none.fl_str_mv WOS:000071594600004
identifier_str_mv Fems Immunology and Medical Microbiology. Hoboken: Wiley-Blackwell, v. 19, n. 4, p. 285-288, 1997.
0928-8244
10.1111/j.1574-695X.1997.tb01098.x
WOS:000071594600004
url http://repositorio.unifesp.br/handle/11600/25814
http://dx.doi.org/10.1111/j.1574-695X.1997.tb01098.x
dc.language.iso.fl_str_mv eng
language eng
dc.relation.ispartof.none.fl_str_mv Fems Immunology and Medical Microbiology
dc.rights.driver.fl_str_mv http://olabout.wiley.com/WileyCDA/Section/id-406071.html
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://olabout.wiley.com/WileyCDA/Section/id-406071.html
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 285-288
dc.publisher.none.fl_str_mv Wiley-Blackwell
publisher.none.fl_str_mv Wiley-Blackwell
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv
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