Immunostimulatory property of a synthetic peptide belonging to the soluble ATP diphosphohydro¬lase isoform (SmATPDase 2) and immunolocalisation of this protein in the Schistosoma mansoni egg
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2011 |
| Outros Autores: | , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/8953 |
Resumo: | Universidade Federal de Juiz de Fora. Instituto de Ciências Biológicas. Departamento de Bioquímica. Juiz de Fora, MG, Brasil. |
| id |
CRUZ_98511746ef73726c7e26c1c91532cb04 |
|---|---|
| oai_identifier_str |
oai:www.arca.fiocruz.br:icict/8953 |
| network_acronym_str |
CRUZ |
| network_name_str |
Repositório Institucional da FIOCRUZ (ARCA) |
| repository_id_str |
2135 |
| spelling |
Mendes, Rita Gabriela Pedrosa RibeiroGusmão, Michélia Antônia do NascimentoMaia, Ana Carolina Ribeiro GomesDetoni, Michelle de LimaPorcino, Gabriane NascimentoSoares, Thais VieiraJuliano, Maria AparecidaJuliano, LuizCoelho, Paulo Marcos ZechLenzi, Henrique LeonelPinto, Priscila de FariaVasconcelos, Eveline Gomes2014-11-24T18:01:13Z2014-11-24T18:01:13Z2011MENDES, Rita Gabriela Pedrosa Ribeiro et al. Immunostimulatory property of a synthetic peptide belonging to the soluble ATP diphosphohydro¬lase isoform (SmATPDase 2) and immunolocalisation of this protein in the Schistosoma mansoni egg. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 106, n. 7, p. 808-813, 2011.0074-0276https://www.arca.fiocruz.br/handle/icict/895310.1590/S0074-02762011000700005engFundação Oswaldo CruzImmunostimulatory property of a synthetic peptide belonging to the soluble ATP diphosphohydro¬lase isoform (SmATPDase 2) and immunolocalisation of this protein in the Schistosoma mansoni egginfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal de Juiz de Fora. Instituto de Ciências Biológicas. Departamento de Bioquímica. Juiz de Fora, MG, Brasil.Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Biofísica. São Paulo, SP, Brasil.Universidade Federal de Juiz de Fora. Instituto de Ciências Biológicas. Departamento de Bioquímica. Juiz de Fora, MG, Brasil.Universidade Federal de Juiz de Fora. Instituto de Ciências Biológicas. Departamento de Bioquímica. Juiz de Fora, MG, Brasil.Universidade Federal de Juiz de Fora. Instituto de Ciências Biológicas. Departamento de Bioquímica. Juiz de Fora, MG, Brasil.Universidade Federal de Juiz de Fora. Instituto de Ciências Biológicas. Departamento de Bioquímica. Juiz de Fora, MG, Brasil.Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Biofísica. São Paulo, SP, Brasil.Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Biofísica. São Paulo, SP, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Patologia. Rio de Janeiro, RJ, Brasil.Universidade Federal de Juiz de Fora. Instituto de Ciências Biológicas. Departamento de Bioquímica. Juiz de Fora, MG, Brasil.Universidade Federal de Juiz de Fora. Instituto de Ciências Biológicas. Departamento de Bioquímica. Juiz de Fora, MG, Brasil.A peptide (SmB2LJ; r175-194) that belongs to a conserved domain from Schistosoma mansoni SmATPDase 2 and is shared with potato apyrase, as predicted by in silico analysis as antigenic, was synthesised and its immunostimulatory property was analysed. When inoculated in BALB/c mice, this peptide induced high levels of SmB2LJ-specific IgG1 and IgG2a subtypes, as detected by enzyme linked immunosorbent assay. In addition, dot blots were found to be positive for immune sera against potato apyrase and SmB2LJ. These results suggest that the conserved domain r175-194 from the S. mansoni SmATPDase 2 is antigenic. Western blots were performed and the anti-SmB2LJ antibody recognised in adult worm (soluble worm antigen preparation) or soluble egg antigen antigenic preparations two bands of approximately 63 and 55 kDa, molecular masses similar to those predicted for adult worm SmATPDase 2. This finding strongly suggests the expression of this same isoform in S. mansoni eggs. To assess localisation of SmATPDase 2, confocal fluorescence microscopy was performed using cryostat sections of infected mouse liver and polyclonal antiserum against SmB2LJ. Positive reactions were identified on the external surface from the miracidium in von Lichtenberg's envelope and, in the outer side of the egg-shell, showing that this soluble isoform is secreted from the S. mansoni eggsSchistosoma mansoniNTPDasepotato apyrasepeptideinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/8953/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALImmunostimulatory property of a synthetic peptide belonging to the soluble ATP diphosphohydro-lase isoform.pdfImmunostimulatory property of a synthetic peptide belonging to the soluble ATP diphosphohydro-lase isoform.pdfapplication/pdf596076https://www.arca.fiocruz.br/bitstream/icict/8953/2/Immunostimulatory%20property%20of%20a%20synthetic%20peptide%20belonging%20to%20the%20soluble%20ATP%20diphosphohydro-lase%20isoform.pdf2af0dd09cfba9f8f950509783d98b10dMD52TEXTImmunostimulatory property of a synthetic peptide belonging to the soluble ATP diphosphohydro-lase isoform.pdf.txtImmunostimulatory property of a synthetic peptide belonging to the soluble ATP diphosphohydro-lase isoform.pdf.txtExtracted texttext/plain33544https://www.arca.fiocruz.br/bitstream/icict/8953/3/Immunostimulatory%20property%20of%20a%20synthetic%20peptide%20belonging%20to%20the%20soluble%20ATP%20diphosphohydro-lase%20isoform.pdf.txte9f7ab01ab00499ee790f2a7227dfc0bMD53icict/89532019-06-20 13:56:51.168oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352019-06-20T16:56:51Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.pt_BR.fl_str_mv |
Immunostimulatory property of a synthetic peptide belonging to the soluble ATP diphosphohydro¬lase isoform (SmATPDase 2) and immunolocalisation of this protein in the Schistosoma mansoni egg |
| title |
Immunostimulatory property of a synthetic peptide belonging to the soluble ATP diphosphohydro¬lase isoform (SmATPDase 2) and immunolocalisation of this protein in the Schistosoma mansoni egg |
| spellingShingle |
Immunostimulatory property of a synthetic peptide belonging to the soluble ATP diphosphohydro¬lase isoform (SmATPDase 2) and immunolocalisation of this protein in the Schistosoma mansoni egg Mendes, Rita Gabriela Pedrosa Ribeiro Schistosoma mansoni NTPDase potato apyrase peptide |
| title_short |
Immunostimulatory property of a synthetic peptide belonging to the soluble ATP diphosphohydro¬lase isoform (SmATPDase 2) and immunolocalisation of this protein in the Schistosoma mansoni egg |
| title_full |
Immunostimulatory property of a synthetic peptide belonging to the soluble ATP diphosphohydro¬lase isoform (SmATPDase 2) and immunolocalisation of this protein in the Schistosoma mansoni egg |
| title_fullStr |
Immunostimulatory property of a synthetic peptide belonging to the soluble ATP diphosphohydro¬lase isoform (SmATPDase 2) and immunolocalisation of this protein in the Schistosoma mansoni egg |
| title_full_unstemmed |
Immunostimulatory property of a synthetic peptide belonging to the soluble ATP diphosphohydro¬lase isoform (SmATPDase 2) and immunolocalisation of this protein in the Schistosoma mansoni egg |
| title_sort |
Immunostimulatory property of a synthetic peptide belonging to the soluble ATP diphosphohydro¬lase isoform (SmATPDase 2) and immunolocalisation of this protein in the Schistosoma mansoni egg |
| author |
Mendes, Rita Gabriela Pedrosa Ribeiro |
| author_facet |
Mendes, Rita Gabriela Pedrosa Ribeiro Gusmão, Michélia Antônia do Nascimento Maia, Ana Carolina Ribeiro Gomes Detoni, Michelle de Lima Porcino, Gabriane Nascimento Soares, Thais Vieira Juliano, Maria Aparecida Juliano, Luiz Coelho, Paulo Marcos Zech Lenzi, Henrique Leonel Pinto, Priscila de Faria Vasconcelos, Eveline Gomes |
| author_role |
author |
| author2 |
Gusmão, Michélia Antônia do Nascimento Maia, Ana Carolina Ribeiro Gomes Detoni, Michelle de Lima Porcino, Gabriane Nascimento Soares, Thais Vieira Juliano, Maria Aparecida Juliano, Luiz Coelho, Paulo Marcos Zech Lenzi, Henrique Leonel Pinto, Priscila de Faria Vasconcelos, Eveline Gomes |
| author2_role |
author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Mendes, Rita Gabriela Pedrosa Ribeiro Gusmão, Michélia Antônia do Nascimento Maia, Ana Carolina Ribeiro Gomes Detoni, Michelle de Lima Porcino, Gabriane Nascimento Soares, Thais Vieira Juliano, Maria Aparecida Juliano, Luiz Coelho, Paulo Marcos Zech Lenzi, Henrique Leonel Pinto, Priscila de Faria Vasconcelos, Eveline Gomes |
| dc.subject.en.pt_BR.fl_str_mv |
Schistosoma mansoni NTPDase potato apyrase peptide |
| topic |
Schistosoma mansoni NTPDase potato apyrase peptide |
| description |
Universidade Federal de Juiz de Fora. Instituto de Ciências Biológicas. Departamento de Bioquímica. Juiz de Fora, MG, Brasil. |
| publishDate |
2011 |
| dc.date.issued.fl_str_mv |
2011 |
| dc.date.accessioned.fl_str_mv |
2014-11-24T18:01:13Z |
| dc.date.available.fl_str_mv |
2014-11-24T18:01:13Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.citation.fl_str_mv |
MENDES, Rita Gabriela Pedrosa Ribeiro et al. Immunostimulatory property of a synthetic peptide belonging to the soluble ATP diphosphohydro¬lase isoform (SmATPDase 2) and immunolocalisation of this protein in the Schistosoma mansoni egg. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 106, n. 7, p. 808-813, 2011. |
| dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/8953 |
| dc.identifier.issn.none.fl_str_mv |
0074-0276 |
| dc.identifier.doi.none.fl_str_mv |
10.1590/S0074-02762011000700005 |
| identifier_str_mv |
MENDES, Rita Gabriela Pedrosa Ribeiro et al. Immunostimulatory property of a synthetic peptide belonging to the soluble ATP diphosphohydro¬lase isoform (SmATPDase 2) and immunolocalisation of this protein in the Schistosoma mansoni egg. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 106, n. 7, p. 808-813, 2011. 0074-0276 10.1590/S0074-02762011000700005 |
| url |
https://www.arca.fiocruz.br/handle/icict/8953 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.publisher.none.fl_str_mv |
Fundação Oswaldo Cruz |
| publisher.none.fl_str_mv |
Fundação Oswaldo Cruz |
| dc.source.none.fl_str_mv |
reponame:Repositório Institucional da FIOCRUZ (ARCA) instname:Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ |
| instname_str |
Fundação Oswaldo Cruz (FIOCRUZ) |
| instacron_str |
FIOCRUZ |
| institution |
FIOCRUZ |
| reponame_str |
Repositório Institucional da FIOCRUZ (ARCA) |
| collection |
Repositório Institucional da FIOCRUZ (ARCA) |
| bitstream.url.fl_str_mv |
https://www.arca.fiocruz.br/bitstream/icict/8953/1/license.txt https://www.arca.fiocruz.br/bitstream/icict/8953/2/Immunostimulatory%20property%20of%20a%20synthetic%20peptide%20belonging%20to%20the%20soluble%20ATP%20diphosphohydro-lase%20isoform.pdf https://www.arca.fiocruz.br/bitstream/icict/8953/3/Immunostimulatory%20property%20of%20a%20synthetic%20peptide%20belonging%20to%20the%20soluble%20ATP%20diphosphohydro-lase%20isoform.pdf.txt |
| bitstream.checksum.fl_str_mv |
7d48279ffeed55da8dfe2f8e81f3b81f 2af0dd09cfba9f8f950509783d98b10d e9f7ab01ab00499ee790f2a7227dfc0b |
| bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 |
| repository.name.fl_str_mv |
Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ) |
| repository.mail.fl_str_mv |
repositorio.arca@fiocruz.br |
| _version_ |
1794075778718105600 |