Adição da vitamina E na criopreservação do sêmen caprino

• Autor(a) principal: Penitente Filho, Jurandy Mauro
• Data de Publicação: 2010
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: LOCUS Repositório Institucional da UFV
• Texto Completo: http://locus.ufv.br/handle/123456789/5662
• topic: Criopreservação; Caprino; Vitamina E; Espermatozóides; Cryopreservation; Goat; Vitamin E; Sperm; CNPQ::CIENCIAS AGRARIAS::ZOOTECNIA::PRODUCAO ANIMAL

The objectives of this study were to examine whether vitamin E has an effect on the structural integrity of goat sperm plasma membrane, as well to investigate the potential use of this vitamin in extenders medium for cryopreservation of goat semen. Two adult Parda Alpina breed males were used, totalizing 8 semen samples for each one. After collection, the physical characteristics of the semen, the sperm morphology and the functional integrity of sperm membrane by hypoosmotic swelling test were evaluated. Then the semen was diluted as follows: BIOXCELL® (Control), BIOXCELL® + Equex, BIOXCELL® + Vitamin E (25μM), BIOXCELL® + Vitamin E (50μM) and BIOXCELL® + Vitamin E (100μM). After the final dilutions, the progressive motility, sperm vigor and hypoosmotic swelling test were performed for each treatment. The semen was packaged in 0.25 ml straws, the straws were cooled to 5 oC for 1 hour in plastic container containing ethyl alcohol. The pre-freezing was done in liquid nitrogen vapor for 15 minutes. After this period, the straws were immersed in liquid nitrogen. The samples were thawed in a water bath at 37 oC for 30 seconds, packaged in plastic tubes and homogenized for immediate analysis of sperm motility and vigor, hypoosmotic swelling test and thermoresistance test. In fresh semen, the physical and morphological characteristics remained within normal parameters. No correlation of motility with other variables were detected. The average volume correlated negatively with sperm vigor, minor defects and total defects (r = -0.55, r = -0.52 and r = -0.53, respectively) and positive correlation with the hypoosmotic swelling test (r = 0, 44). A negative correlation (r = -0.48) was found between sperm concentration and major defects. The minor defects and total defects values were markedly positive correlated (r = 0.98). The treatments did not differ (P > 0.05) for progressive motility, spermatic vigor and hypoosmotic swelling test of semen. The motility and vigor after thawing and during the TTR did not differ (P > 0.05) among treatments. No significant difference (P > 0.05) among treatments after the completion of the hypoosmotic swelling test was detected. Correlation was observed between motility and vigor in all treatments in the diluted semen (pre-cooled) and at all times of the TTR. No correlation between the vigor and the hypoosmotic swelling test in any of the treatments on 0H and 2H of TTR times was found, however a correlation at 1H time Equex treatment (r = 0.51) and the Control, Equex and Vit. E 50μM treatment at time 3H (r = 0.44, 0.69, 0.57, respectively) were found. The thawed semen samples from Equex treatment and Vit. E 100μM treatment showed correlation between motility and hypoosmotic swelling test. It is concluded that: The vitamin E did not affect any of parameters under study.