Evidence for the association of the human regulatory protein Ki-1/57 with the translational machinery

• Autor(a) principal: Gonçalves, Kaliandra de Almeida
• Data de Publicação: 2011
• Outros Autores: Bressan, Gustavo Costa, Saito, Ângela, Morello, Luis Gustavo, Zanchin, Nilson Ivo T., Kobarg, Jörg
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: LOCUS Repositório Institucional da UFV
• Texto Completo: https://doi.org/10.1016/j.febslet.2011.07.010; http://www.locus.ufv.br/handle/123456789/21302
• topic: Regulatory protein; Protein–protein interaction; Translation

Ki-1/57 is a cytoplasmic and nuclear protein of 57 kDa first identified in malignant cells from Hodgkin’s lymphoma. Based on yeast-two hybrid protein interaction we found out that Ki-1/57 interacts with adaptor protein RACK1 (receptor of activated kinase 1), CIRP (cold-inducible RNA-binding protein), RPL38 (ribosomal protein L38) and FXR1 (fragile X mental retardation-related protein 1). Since these proteins are involved in the regulation of translation we suspected that Ki-1/57 may have a role in it. We show by immunoprecipitation the association of Ki-1/57 with FMRP. Confocal microscopy revealed that Ki-1/57 colocalizes with FMRP/FXR1/2 to stress granules. Furthermore Ki-1/57 cosediments with free ribosomal particles and enhances translation, when tethered to a reporter mRNA, suggesting that Ki-1/57 may be involved in translational regulation.