Características reprodutivas de garanhões da raça Mangalarga Marchador em diferentes faixas etárias
Autor(a) principal: | |
---|---|
Data de Publicação: | 2014 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | LOCUS Repositório Institucional da UFV |
Texto Completo: | http://locus.ufv.br/handle/123456789/1889 |
Resumo: | The Breeders Association of Mangalarga Machador race is the largest in Latin America; however, the reproductive characteristics are not complete known for stallions of different ages. The aim was to evaluate the main reproductive characteristics of Mangalarga Marchador stallions as follows: pre-breeding behavior, testicular biometry, characteristics of accessory glands, seminal quality and semen resistance to cooling and freezing. Experiment 1: 110 stallions were evaluated to measure the reproductive behavior (time to react, latency time, breeding time, duration of semen collection and number of Flehmen reflex) during two consecutive semen collections (C1 and C2) with one hour-interval. The stallions were grouped according to four age intervals: G1: 2-3 years old, G2: 4-6 years, G3: 7-14 years, and G4: older than 15 years. The averaged time for semen collection was 162 sec for C1 and 171.9 sec for C2. No difference was detected for reproductive behavior characteristics among the four age categories. Experiment 2: testicular biometry was evaluated in 101 stallions. The averaged scrotal diameter was reduced in G1 stallions (88.7 mm) compared to G2 (94.5 mm), and was also reduced in G2 stallions compared to G3 (104.1 mm) and G4 (108.6 mm; P<0.05). An age effect was also observed for some accessory glands. During testicular evaluation was detected hydrocele and testicular rotation in 10 and 12 % of stallions, respectively. Experiment 3: two consecutives semen collections were done in 107 stallions to evaluate the semen characteristics (volume with and without gel, total motility, progressive motility, vigor, sperm concentration, total number of sperm, morphologic alterations, and total number of motile and normal sperm (TMNS). The TMNS was affected by the age categories in C1; TMNS was lower in the G1 (2.8 billions) and G2 stallions (3.6 billions) than in the G3 (5.4 billions). Experiment 4: semen collections of 94 stallions were used for evaluation of semen resistance to cooling in two different temperatures (15oC/12 hours, R12 ; and 5oC/24 hours, R24) and to freezing. Cooled semen was evaluated by a subjective analysis to measure the total (TM) and progressive motility (MP) and vigor of sperm. The plasma membrane integrity (PMI) was also evaluated by the supravital test. Frozen-thawed semen was submitted to a computerized analysis to evaluated motility parameters. No difference was detected among the stallions age categories (P>0.05). The averaged TM, PM, vigor and PMI were respectively: 64.4%, 56.1%, 3.2 and 70.4% for R12 (n=71), and 59.3%, 52.5%, 2.8 and 67.2% for R24 (n=77). The PM was superior to 35% in 89% after cooling to 15oC/12 hours (R12). For the frozen-thawed semen, the averaged values of TM, PM and VAP were respectively: 60.9%, 43.8% and 77.6 um/sec in C1 (n=89), and 61.4%, 44.5% and 79.1 um/sec in C2 (n=78). The PM was superior to 35% in 73% frozen-thawed semen. In conclusion, the reproductive characteristics of stallions of Mangalarga Marchador breed are compatible in the normal breeding behavior and semen quality aspects reported for horses. Stallion s age may affect some characteristics. The high percentage of stallions with high resistance of semen to cooling and freezing procedures indicates that these techniques may be useful in large scale in the routine and among breeders of Mangalarga Marchador breed. |
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Oliveira, Renan Reis dehttp://lattes.cnpq.br/6418065105158213Guimarães, José Domingoshttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4782270U6Torres, Ciro Alexandre Alveshttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4787213D4Carvalho, Giovanni Ribeiro dehttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4723068Z6Costa, Eduardo Paulino dahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4787237D6Santos, Giancarlo Magalhães doshttp://lattes.cnpq.br/33650203924121702015-03-26T12:55:01Z2015-01-162015-03-26T12:55:01Z2014-07-14OLIVEIRA, Renan Reis de. Relationship between reproductive characteristics and age of Mangalarga Marchador breed stallion. 2014. 99 f. Tese (Doutorado em Genética e Melhoramento de Animais Domésticos; Nutrição e Alimentação Animal; Pastagens e Forragicul) - Universidade Federal de Viçosa, Viçosa, 2014.http://locus.ufv.br/handle/123456789/1889The Breeders Association of Mangalarga Machador race is the largest in Latin America; however, the reproductive characteristics are not complete known for stallions of different ages. The aim was to evaluate the main reproductive characteristics of Mangalarga Marchador stallions as follows: pre-breeding behavior, testicular biometry, characteristics of accessory glands, seminal quality and semen resistance to cooling and freezing. Experiment 1: 110 stallions were evaluated to measure the reproductive behavior (time to react, latency time, breeding time, duration of semen collection and number of Flehmen reflex) during two consecutive semen collections (C1 and C2) with one hour-interval. The stallions were grouped according to four age intervals: G1: 2-3 years old, G2: 4-6 years, G3: 7-14 years, and G4: older than 15 years. The averaged time for semen collection was 162 sec for C1 and 171.9 sec for C2. No difference was detected for reproductive behavior characteristics among the four age categories. Experiment 2: testicular biometry was evaluated in 101 stallions. The averaged scrotal diameter was reduced in G1 stallions (88.7 mm) compared to G2 (94.5 mm), and was also reduced in G2 stallions compared to G3 (104.1 mm) and G4 (108.6 mm; P<0.05). An age effect was also observed for some accessory glands. During testicular evaluation was detected hydrocele and testicular rotation in 10 and 12 % of stallions, respectively. Experiment 3: two consecutives semen collections were done in 107 stallions to evaluate the semen characteristics (volume with and without gel, total motility, progressive motility, vigor, sperm concentration, total number of sperm, morphologic alterations, and total number of motile and normal sperm (TMNS). The TMNS was affected by the age categories in C1; TMNS was lower in the G1 (2.8 billions) and G2 stallions (3.6 billions) than in the G3 (5.4 billions). Experiment 4: semen collections of 94 stallions were used for evaluation of semen resistance to cooling in two different temperatures (15oC/12 hours, R12 ; and 5oC/24 hours, R24) and to freezing. Cooled semen was evaluated by a subjective analysis to measure the total (TM) and progressive motility (MP) and vigor of sperm. The plasma membrane integrity (PMI) was also evaluated by the supravital test. Frozen-thawed semen was submitted to a computerized analysis to evaluated motility parameters. No difference was detected among the stallions age categories (P>0.05). The averaged TM, PM, vigor and PMI were respectively: 64.4%, 56.1%, 3.2 and 70.4% for R12 (n=71), and 59.3%, 52.5%, 2.8 and 67.2% for R24 (n=77). The PM was superior to 35% in 89% after cooling to 15oC/12 hours (R12). For the frozen-thawed semen, the averaged values of TM, PM and VAP were respectively: 60.9%, 43.8% and 77.6 um/sec in C1 (n=89), and 61.4%, 44.5% and 79.1 um/sec in C2 (n=78). The PM was superior to 35% in 73% frozen-thawed semen. In conclusion, the reproductive characteristics of stallions of Mangalarga Marchador breed are compatible in the normal breeding behavior and semen quality aspects reported for horses. Stallion s age may affect some characteristics. The high percentage of stallions with high resistance of semen to cooling and freezing procedures indicates that these techniques may be useful in large scale in the routine and among breeders of Mangalarga Marchador breed.A Associação de Criadores da raça Mangalarga Machador é a maior da America Latina, no entanto, pouco se sabe a respeitos dos aspectos reprodutivos de características seus garanhões. reprodutivas de Objetivou-se garanhões da avaliar raça algumas Mangalarga Marchador, dentre elas: comportamento pré cópula, biometria testicular e das glândulas acessórias, parâmetros seminais, resistência ao refrigeramento e congelamento; assim como verificar efeito de diferentes faixas etárias nestas características. Experimento 1: 110 garanhões tiveram características comportamentais (tempo de reação, tempo de latência, tempo de monta, duração total da colheita e número de reflexos de flehmen) analisadas durante procedimento de duas colheitas de sêmen (C1 e C2) intervaladas de uma hora. Os animais foram agrupados nas seguintes faixas etárias: G1: 2-3 anos, G2: 4-6 anos, G3: 7-14 anos e G4: mais de 15 anos. O tempo médio de C1 foi de 162 s. e de C2 de 171,9 s. Não foi observado diferenças nas características comportamentais para as diferentes faixas etárias. Experimento 2: 101 garanhões tiveram aferidas medidas relativas: à biometria testicular (comprimento, largura e altura dos testículos direito e esquerdo, e largura testicular total), às glândulas acessórias (bulbo uretral, próstata e vesicular) e ampolas do ducto deferente. As médias do largura escrotal total de G1 (88,7 mm) foram inferiores a G2 (94,5 mm) e as de G2 inferiores a de G3 (104,1 mm) e G4 (108,6 mm) (P<0,05) que foram iguais (P>0,05). Foi observado efeito de idade para as mensurações de algumas glândulas acessórias, como, bulbo uretrais, largura do lobo prostático e altura de ampola do ducto deferente (P<0,05) dentre as faixas etárias analisadas. Observou-se incidência de hidrocele testicular e torção testicular em 10 e 12%, respectivamente, dos animais avaliados. Experimento 3: 107 garanhões foram submetidos a duas colheitas de sêmen com intervalo de uma hora para avaliação dos seguintes parâmetros: volume de gel, volume sem gel, motilidade total, motilidade progressiva, vigor, concentração, número total de espermatozoides, defeitos morfológicos e número total de espermatozoides móveis e normais (TSMN). O TSMN no ejaculado apresentou efeito das diferentes faixas etárias para C1, G1 (2,8 bilhões), G2 (3,6 bilhões) apresentaram valores inferiores a G3 (5,4 bilhões) (P<0,05). Não houve diferença entre G4 (2,7 bilhões) e os demais grupos (P>0,05). Experimento 4: 94 garanhões tiveram o sêmen colheitado e processado pra refrigeramento (R12 15 oC/12 horas e R24 5 oC/24 horas) e congelamento (duas colheitas com intervalo de uma hora: C1 e C2). O sêmen refrigerado foi avaliado quanto aos parâmetros físicos (análise subjetiva: MT- motilidade total, MP - motilidade progressiva e vigor) e integridade da membrana plasmática (IMP). O sêmen congelado foi submetido à avaliação computadorizada do movimento espermático. Não foi observado efeito das diferentes faixas etárias para os parâmetros avaliados (P>0,05). Em R12 (n=71) e R24 (n=77) obteve-se MT, MP, vigor e IMP valores médios de: 64,4%, 56,1%, respectivamente. 3,2, Ainda, 70,4%, 89% 59,3%, 52,5%, 2,8 (R12) e 87% (R24) e 67,2%, dos animais apresentaram acima de 35% de MP após respectivos períodos de refrigeramento. C1 (n=89) e C2 (n=78) apresentaram valores médios de MT, MP e VAP para C1 e C2, respectivamente de: 60,9, 43,8, 77,6, 61,4, 44,5 e 79,1. Dos 94 animais avaliados 73% apresentaram motilidade progressiva superior a 35%. Os parâmetros reprodutivos de garanhões da raça Mangalarga Marchador encontram-se dentro dos parâmetros descritos para espécie, sendo observados alguns efeitos de idade para estes parâmetros. A resistência ao refrigeramento e congelamento se mostraram satisfatórias podendo tais ferramentas serem aplicadas na rotina de profissionais que trabalham com esta raça.Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorapplication/pdfporUniversidade Federal de ViçosaDoutorado em ZootecniaUFVBRGenética e Melhoramento de Animais Domésticos; Nutrição e Alimentação Animal; Pastagens e ForragiculMangalarga (cavalo)Cavalo - ReproduçãoAndrologiaSêmen - CriopreservaçãoMangalarga (horse)Horse - ReproductionAndrologySemen - CryopreservationCNPQ::CIENCIAS AGRARIAS::ZOOTECNIACaracterísticas reprodutivas de garanhões da raça Mangalarga Marchador em diferentes faixas etáriasRelationship between reproductive characteristics and age of Mangalarga Marchador breed stallioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisinfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALtexto completo.pdfapplication/pdf836185https://locus.ufv.br//bitstream/123456789/1889/1/texto%20completo.pdf42d517b3c26df705c654aeb00a8e2f73MD51TEXTtexto completo.pdf.txttexto completo.pdf.txtExtracted texttext/plain177703https://locus.ufv.br//bitstream/123456789/1889/2/texto%20completo.pdf.txt2e9d83f07a9a244142c8e400dc987ff6MD52THUMBNAILtexto completo.pdf.jpgtexto completo.pdf.jpgIM Thumbnailimage/jpeg3525https://locus.ufv.br//bitstream/123456789/1889/3/texto%20completo.pdf.jpg65718db31d79c6e9ae2c5a3681a713a9MD53123456789/18892016-04-07 23:17:19.348oai:locus.ufv.br:123456789/1889Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452016-04-08T02:17:19LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false |
dc.title.por.fl_str_mv |
Características reprodutivas de garanhões da raça Mangalarga Marchador em diferentes faixas etárias |
dc.title.alternative.eng.fl_str_mv |
Relationship between reproductive characteristics and age of Mangalarga Marchador breed stallion |
title |
Características reprodutivas de garanhões da raça Mangalarga Marchador em diferentes faixas etárias |
spellingShingle |
Características reprodutivas de garanhões da raça Mangalarga Marchador em diferentes faixas etárias Oliveira, Renan Reis de Mangalarga (cavalo) Cavalo - Reprodução Andrologia Sêmen - Criopreservação Mangalarga (horse) Horse - Reproduction Andrology Semen - Cryopreservation CNPQ::CIENCIAS AGRARIAS::ZOOTECNIA |
title_short |
Características reprodutivas de garanhões da raça Mangalarga Marchador em diferentes faixas etárias |
title_full |
Características reprodutivas de garanhões da raça Mangalarga Marchador em diferentes faixas etárias |
title_fullStr |
Características reprodutivas de garanhões da raça Mangalarga Marchador em diferentes faixas etárias |
title_full_unstemmed |
Características reprodutivas de garanhões da raça Mangalarga Marchador em diferentes faixas etárias |
title_sort |
Características reprodutivas de garanhões da raça Mangalarga Marchador em diferentes faixas etárias |
author |
Oliveira, Renan Reis de |
author_facet |
Oliveira, Renan Reis de |
author_role |
author |
dc.contributor.authorLattes.por.fl_str_mv |
http://lattes.cnpq.br/6418065105158213 |
dc.contributor.author.fl_str_mv |
Oliveira, Renan Reis de |
dc.contributor.advisor-co1.fl_str_mv |
Guimarães, José Domingos |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4782270U6 |
dc.contributor.advisor-co2.fl_str_mv |
Torres, Ciro Alexandre Alves |
dc.contributor.advisor-co2Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4787213D4 |
dc.contributor.advisor1.fl_str_mv |
Carvalho, Giovanni Ribeiro de |
dc.contributor.advisor1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4723068Z6 |
dc.contributor.referee1.fl_str_mv |
Costa, Eduardo Paulino da |
dc.contributor.referee1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4787237D6 |
dc.contributor.referee2.fl_str_mv |
Santos, Giancarlo Magalhães dos |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/3365020392412170 |
contributor_str_mv |
Guimarães, José Domingos Torres, Ciro Alexandre Alves Carvalho, Giovanni Ribeiro de Costa, Eduardo Paulino da Santos, Giancarlo Magalhães dos |
dc.subject.por.fl_str_mv |
Mangalarga (cavalo) Cavalo - Reprodução Andrologia Sêmen - Criopreservação |
topic |
Mangalarga (cavalo) Cavalo - Reprodução Andrologia Sêmen - Criopreservação Mangalarga (horse) Horse - Reproduction Andrology Semen - Cryopreservation CNPQ::CIENCIAS AGRARIAS::ZOOTECNIA |
dc.subject.eng.fl_str_mv |
Mangalarga (horse) Horse - Reproduction Andrology Semen - Cryopreservation |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS AGRARIAS::ZOOTECNIA |
description |
The Breeders Association of Mangalarga Machador race is the largest in Latin America; however, the reproductive characteristics are not complete known for stallions of different ages. The aim was to evaluate the main reproductive characteristics of Mangalarga Marchador stallions as follows: pre-breeding behavior, testicular biometry, characteristics of accessory glands, seminal quality and semen resistance to cooling and freezing. Experiment 1: 110 stallions were evaluated to measure the reproductive behavior (time to react, latency time, breeding time, duration of semen collection and number of Flehmen reflex) during two consecutive semen collections (C1 and C2) with one hour-interval. The stallions were grouped according to four age intervals: G1: 2-3 years old, G2: 4-6 years, G3: 7-14 years, and G4: older than 15 years. The averaged time for semen collection was 162 sec for C1 and 171.9 sec for C2. No difference was detected for reproductive behavior characteristics among the four age categories. Experiment 2: testicular biometry was evaluated in 101 stallions. The averaged scrotal diameter was reduced in G1 stallions (88.7 mm) compared to G2 (94.5 mm), and was also reduced in G2 stallions compared to G3 (104.1 mm) and G4 (108.6 mm; P<0.05). An age effect was also observed for some accessory glands. During testicular evaluation was detected hydrocele and testicular rotation in 10 and 12 % of stallions, respectively. Experiment 3: two consecutives semen collections were done in 107 stallions to evaluate the semen characteristics (volume with and without gel, total motility, progressive motility, vigor, sperm concentration, total number of sperm, morphologic alterations, and total number of motile and normal sperm (TMNS). The TMNS was affected by the age categories in C1; TMNS was lower in the G1 (2.8 billions) and G2 stallions (3.6 billions) than in the G3 (5.4 billions). Experiment 4: semen collections of 94 stallions were used for evaluation of semen resistance to cooling in two different temperatures (15oC/12 hours, R12 ; and 5oC/24 hours, R24) and to freezing. Cooled semen was evaluated by a subjective analysis to measure the total (TM) and progressive motility (MP) and vigor of sperm. The plasma membrane integrity (PMI) was also evaluated by the supravital test. Frozen-thawed semen was submitted to a computerized analysis to evaluated motility parameters. No difference was detected among the stallions age categories (P>0.05). The averaged TM, PM, vigor and PMI were respectively: 64.4%, 56.1%, 3.2 and 70.4% for R12 (n=71), and 59.3%, 52.5%, 2.8 and 67.2% for R24 (n=77). The PM was superior to 35% in 89% after cooling to 15oC/12 hours (R12). For the frozen-thawed semen, the averaged values of TM, PM and VAP were respectively: 60.9%, 43.8% and 77.6 um/sec in C1 (n=89), and 61.4%, 44.5% and 79.1 um/sec in C2 (n=78). The PM was superior to 35% in 73% frozen-thawed semen. In conclusion, the reproductive characteristics of stallions of Mangalarga Marchador breed are compatible in the normal breeding behavior and semen quality aspects reported for horses. Stallion s age may affect some characteristics. The high percentage of stallions with high resistance of semen to cooling and freezing procedures indicates that these techniques may be useful in large scale in the routine and among breeders of Mangalarga Marchador breed. |
publishDate |
2014 |
dc.date.issued.fl_str_mv |
2014-07-14 |
dc.date.accessioned.fl_str_mv |
2015-03-26T12:55:01Z |
dc.date.available.fl_str_mv |
2015-01-16 2015-03-26T12:55:01Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
OLIVEIRA, Renan Reis de. Relationship between reproductive characteristics and age of Mangalarga Marchador breed stallion. 2014. 99 f. Tese (Doutorado em Genética e Melhoramento de Animais Domésticos; Nutrição e Alimentação Animal; Pastagens e Forragicul) - Universidade Federal de Viçosa, Viçosa, 2014. |
dc.identifier.uri.fl_str_mv |
http://locus.ufv.br/handle/123456789/1889 |
identifier_str_mv |
OLIVEIRA, Renan Reis de. Relationship between reproductive characteristics and age of Mangalarga Marchador breed stallion. 2014. 99 f. Tese (Doutorado em Genética e Melhoramento de Animais Domésticos; Nutrição e Alimentação Animal; Pastagens e Forragicul) - Universidade Federal de Viçosa, Viçosa, 2014. |
url |
http://locus.ufv.br/handle/123456789/1889 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
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openAccess |
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Universidade Federal de Viçosa |
dc.publisher.program.fl_str_mv |
Doutorado em Zootecnia |
dc.publisher.initials.fl_str_mv |
UFV |
dc.publisher.country.fl_str_mv |
BR |
dc.publisher.department.fl_str_mv |
Genética e Melhoramento de Animais Domésticos; Nutrição e Alimentação Animal; Pastagens e Forragicul |
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Universidade Federal de Viçosa |
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