Fertilidade e morfofisiologia epididimária de ratos wistar submetidos a ingestão de arsenato e arsenito de sódio

• Autor(a) principal: Lima, Graziela Domingues de Almeida
• Data de Publicação: 2013
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: LOCUS Repositório Institucional da UFV
• Texto Completo: http://locus.ufv.br/handle/123456789/2363
• topic: Arsênio; Epidídimo; Histomorfometria; Trânsito epididimário; Espermatozoides; Taxa de gestação; Arsenic; Epididymis; Histomorfometria; Epididymal transit; Sperm; Pregnancy rate; CNPQ::CIENCIAS BIOLOGICAS::BIOLOGIA GERAL

The aim of this study was to compare the effect of exposure to different concentrations of arsenic in the pentavalent (arsenate, As+5) and trivalent forms (arsenite, As+3) on reproductive parameters. Were used 30 Wistar rats randomly divided into five experimental groups (n = 6 animals/group). The control animals received saline, while the treated animals were exposed orally to sodium arsenate and arsenite, testing for each chemical form with concentrations of 0.01mg/L and 10mg/L, daily for 56 days. Animals exposed to 10mg/L of arsenite had reduced daily sperm production, the number of spermatids in the testis and per gram of testis, the number of sperm in the epididymal regions and the percentage of sperm with intact membranes (P < 0.05). Already 0.01mg/L arsenite caused a reduction in the number of sperm in the caput/corpus regions of the epididymis and the percentage of sperm with intact membranes (P < 0.05). In addition, there was increase in the percentage of blood vessels in the initial segment of the epididymis to the animals exposed to arsenite (0.01 and 10mg/L) and arsenate (0.01mg/L). Sodium arsenate at the concentration to 10mg/L, only changed the percentage of sperm with intact membranes and percentage of lumen without sperm in the cauda of the epididymis (P < 0.05). Regarding the fertility parameters, there was obtained 81.82% of pregnant females to males control, 50% of males exposed with arsenate 0.01 mg/L and arsenite 10 mg/L and 63.64% of males exposed to 10mg/L of arsenate. 81.8% of the females conceived from males, 50% when the males were treated with 0.01 mg/L of arsenate and 10 mg/L of arsenite and 63.6% when 10 mg/L of arsenate were used. There was no difference between females groups for number of corpora lutea and implantations (P > 0.05), and the fertility potential (%) for the males exposed with arsenate 0.01mg/L (40.6 ± 12.8) and arsenite 10mg/L (40.6 ±11.7) was lower than the control males (71.0 ± 21.4). The pre-implantation loss was 59% for males exposed to arsenite 10mg/L and arsenate 0.01mg/L and 29% for control animals. In conclusion, the arsenite was more damaging than sodium arsenate some parameters for testicular and epididymal sperm. Arsenic present in drinking water at 0.01mg/L altered fertility parameters in the form of sodium arsenate