Mapeamento de loco de resistência à Puccinia psidii em Eucalyptus sp. por meio de marcadores moleculares microssatélites

• Autor(a) principal: Tomaz, Rafael Simões
• Data de Publicação: 2008
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: LOCUS Repositório Institucional da UFV
• Texto Completo: http://locus.ufv.br/handle/123456789/4684
• topic: Eucalyptus; Mapeamento genômico; QTL; Eucalyptus; Genomic mapping; QTL; CNPQ::CIENCIAS BIOLOGICAS::GENETICA::GENETICA VEGETAL

The occurrence of the rust caused by Puccinia Psidii can represent a limiting factor for the eucalypt plantations. Planting of resistant genotypes is the most advisable control measure against the disease. This way, the study and the detection of genes that are responsible for the rust resistance, aiming the selection of resistant genotypes, became desirable. This study has the purpose of detect and the map locus responsible to the rust resistance caused by Puccinia Psidii in an interspecific cross of Eucalyptus sp., by using molecular microsatellites markers. Phenotypic information was evaluated from 115 individuals derived progeny resulting from the crossing of a feminine resistant genitor E. urophylla and a masculine hybrid genitor between E. urophylla and E. grandis, as well genotypic information about 22 microsatellites markers. QTL detection was accomplished by using ANOVA, original and modified (weighted by marker informativity) Haseman & Elston regression and Fulker & Cardon regression. Single markers methodologies detected significant association for markers EMBRA 125 and EMBRA 321, both of the linkage group three. The Haseman & Elston regression showed the values of pF = 20,6515 and H2 = 14,73% for the EMBRA 125 marker pF = 70,1694 and H2 = 40,58 for the EMBRA 321 marker. Additionally, the Fulker and Cardon regression allowed the detection of the with QTL with pF = 29,4788 and H2 = 17,58%. The QTL locus was positioned 0,01cM from EMBRA 125. The EMBRA 321 marker could not be evaluated by mean the interval methodology. The application of the markers EMBRA 125 and EMBRA 321 in procedures of assisted selection is putative and it should be investigated.