Estrutura e ultra-estrutura dos espermatozoides de Diaphorina citri (Hemiptera, Sternorryncha, Liviidae)

• Autor(a) principal: Barcellos, Marcelo Silva
• Data de Publicação: 2014
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: LOCUS Repositório Institucional da UFV
• Texto Completo: http://locus.ufv.br/handle/123456789/2374
• topic: Diaphorina citri; Cigarra - Espermatozoides; Cigarra- Doenças e pragas; Diaphorina citri; Cicada - Sperm; Cicada - Diseases and pests; CNPQ::CIENCIAS BIOLOGICAS::BIOLOGIA GERAL

The Hemiptera comprises a diverse group of insects with approximately 89,000 described species and distributed throughout the world. Currently, four suborders of Hemiptera are recognized: Heteroptera, Sternorryncha, Coleorryncha and Auchenorryncha. The suborder Sternorryncha form a monophyletic group with approximately 16,000 described species, distributed in four superfamilies: Aleyrodoidea, Aphidoidea, Coccoidea and Psyloidea. Some species of this suborder are among the major pests of agriculture worldwide. One example is Diaphorina citri Kuwayama (Liviidae), popularly known as the leafhopper, which today is one of the most important and devastating citrus pest worldwide. Considering knowledge about the reproductive biology as well as systematic may contribute to the management of pest, in this paper we describe the structure and ultrastructure of spermatozoa of D. citri. For this, were used light, transmission and scanning electronic microscopy. In this species we observed that the sperm measure about 525 μm in length, are free in the seminal vesicle and do not show polymorphism. As observed in other Psyloidea, the sperm when manipulated opens into two filaments, one in which its anterior end is attached to the nuclei and the other free. Through the flagellum there are lateral projections with about 2 μm in length, this characteristic is reported for another Psyloidea and Aleyrodoidea. Furthermore, at the end of the flagellum three appendages are observed with approximately 7 μm, which were observed so far only in D. citri. The head region is formed by nuclei with compact chromatin and a structure comprising by a matherial with median electron density and it extends extends about 25 μm in length in front of the nuclei. The flagellum consists of an axoneme (9 + 9 + 2), two mitochondrial derivatives and two accessory bodies. The presence of lateral projections is a characteristic shared by Psyloidea and Aleirodoidea, already the sperm opening in two filaments when manipulated occurs only in Psyloidea, and the presence of the three appendices at the end of the flagellum distinguishes D. citri from the other Psyloidea species studied.