Sucrose breakdown within guard cells provides substrates for glycolysis and glutamine biosynthesis during light- induced stomatal opening
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | LOCUS Repositório Institucional da UFV |
Texto Completo: | https://doi.org/10.1111/tpj.13889 http://www.locus.ufv.br/handle/123456789/24494 |
Resumo: | Sucrose has long been thought to play an osmolytic role in stomatal opening. However, recent evidence supports the idea that the role of sucrose in this process is primarily energetic. Here we used a combination of stomatal aperture assays and kinetic [U- 13 C]-sucrose isotope labelling experiments to confirm that sucrose is degraded during light-induced stomatal opening and to define the fate of the C released from sucrose breakdown. We additionally show that addition of sucrose to the medium did not enhance light- induced stomatal opening. The isotope experiment showed a consistent 13 C enrichment in fructose and glu- cose, indicating that during light-induced stomatal opening sucrose is indeed degraded. We also observed a clear 13 C enrichment in glutamate and glutamine (Gln), suggesting a concerted activation of sucrose degra- dation, glycolysis and the tricarboxylic acid cycle. This is in contrast to the situation for Gln biosynthesis in leaves under light, which has been demonstrated to rely on previously stored C. Our results thus collectively allow us to redraw current models concerning the influence of sucrose during light-induced stomatal open- ing, in which, instead of being accumulated, sucrose is degraded providing C skeletons for Gln biosynthesis. |
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Medeiros, David B.Souza, Leonardo PerezAntunes, Werner C.Araújo, Wagner L.Daloso, Danilo M.Fernie, Alisdair R.2019-04-11T16:44:49Z2019-04-11T16:44:49Z2018-051365-313Xhttps://doi.org/10.1111/tpj.13889http://www.locus.ufv.br/handle/123456789/24494Sucrose has long been thought to play an osmolytic role in stomatal opening. However, recent evidence supports the idea that the role of sucrose in this process is primarily energetic. Here we used a combination of stomatal aperture assays and kinetic [U- 13 C]-sucrose isotope labelling experiments to confirm that sucrose is degraded during light-induced stomatal opening and to define the fate of the C released from sucrose breakdown. We additionally show that addition of sucrose to the medium did not enhance light- induced stomatal opening. The isotope experiment showed a consistent 13 C enrichment in fructose and glu- cose, indicating that during light-induced stomatal opening sucrose is indeed degraded. We also observed a clear 13 C enrichment in glutamate and glutamine (Gln), suggesting a concerted activation of sucrose degra- dation, glycolysis and the tricarboxylic acid cycle. This is in contrast to the situation for Gln biosynthesis in leaves under light, which has been demonstrated to rely on previously stored C. Our results thus collectively allow us to redraw current models concerning the influence of sucrose during light-induced stomatal open- ing, in which, instead of being accumulated, sucrose is degraded providing C skeletons for Gln biosynthesis.engThe Plant Journalv. 94, n. 4, p. 583-594, maio 2018Stomatal movementsSucroseGuard cell metabolismTCA cycleGlycolysisStable isotope label- ling analysisSucrose breakdown within guard cells provides substrates for glycolysis and glutamine biosynthesis during light- induced stomatal openinginfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfinfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALartigo.pdfartigo.pdfartigoapplication/pdf441974https://locus.ufv.br//bitstream/123456789/24494/1/artigo.pdf62ecda4b906a9f180b490d9db89c7bb5MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://locus.ufv.br//bitstream/123456789/24494/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52123456789/244942019-04-11 13:46:49.261oai:locus.ufv.br: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Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452019-04-11T16:46:49LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false |
dc.title.en.fl_str_mv |
Sucrose breakdown within guard cells provides substrates for glycolysis and glutamine biosynthesis during light- induced stomatal opening |
title |
Sucrose breakdown within guard cells provides substrates for glycolysis and glutamine biosynthesis during light- induced stomatal opening |
spellingShingle |
Sucrose breakdown within guard cells provides substrates for glycolysis and glutamine biosynthesis during light- induced stomatal opening Medeiros, David B. Stomatal movements Sucrose Guard cell metabolism TCA cycle Glycolysis Stable isotope label- ling analysis |
title_short |
Sucrose breakdown within guard cells provides substrates for glycolysis and glutamine biosynthesis during light- induced stomatal opening |
title_full |
Sucrose breakdown within guard cells provides substrates for glycolysis and glutamine biosynthesis during light- induced stomatal opening |
title_fullStr |
Sucrose breakdown within guard cells provides substrates for glycolysis and glutamine biosynthesis during light- induced stomatal opening |
title_full_unstemmed |
Sucrose breakdown within guard cells provides substrates for glycolysis and glutamine biosynthesis during light- induced stomatal opening |
title_sort |
Sucrose breakdown within guard cells provides substrates for glycolysis and glutamine biosynthesis during light- induced stomatal opening |
author |
Medeiros, David B. |
author_facet |
Medeiros, David B. Souza, Leonardo Perez Antunes, Werner C. Araújo, Wagner L. Daloso, Danilo M. Fernie, Alisdair R. |
author_role |
author |
author2 |
Souza, Leonardo Perez Antunes, Werner C. Araújo, Wagner L. Daloso, Danilo M. Fernie, Alisdair R. |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Medeiros, David B. Souza, Leonardo Perez Antunes, Werner C. Araújo, Wagner L. Daloso, Danilo M. Fernie, Alisdair R. |
dc.subject.pt-BR.fl_str_mv |
Stomatal movements Sucrose Guard cell metabolism TCA cycle Glycolysis Stable isotope label- ling analysis |
topic |
Stomatal movements Sucrose Guard cell metabolism TCA cycle Glycolysis Stable isotope label- ling analysis |
description |
Sucrose has long been thought to play an osmolytic role in stomatal opening. However, recent evidence supports the idea that the role of sucrose in this process is primarily energetic. Here we used a combination of stomatal aperture assays and kinetic [U- 13 C]-sucrose isotope labelling experiments to confirm that sucrose is degraded during light-induced stomatal opening and to define the fate of the C released from sucrose breakdown. We additionally show that addition of sucrose to the medium did not enhance light- induced stomatal opening. The isotope experiment showed a consistent 13 C enrichment in fructose and glu- cose, indicating that during light-induced stomatal opening sucrose is indeed degraded. We also observed a clear 13 C enrichment in glutamate and glutamine (Gln), suggesting a concerted activation of sucrose degra- dation, glycolysis and the tricarboxylic acid cycle. This is in contrast to the situation for Gln biosynthesis in leaves under light, which has been demonstrated to rely on previously stored C. Our results thus collectively allow us to redraw current models concerning the influence of sucrose during light-induced stomatal open- ing, in which, instead of being accumulated, sucrose is degraded providing C skeletons for Gln biosynthesis. |
publishDate |
2018 |
dc.date.issued.fl_str_mv |
2018-05 |
dc.date.accessioned.fl_str_mv |
2019-04-11T16:44:49Z |
dc.date.available.fl_str_mv |
2019-04-11T16:44:49Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://doi.org/10.1111/tpj.13889 http://www.locus.ufv.br/handle/123456789/24494 |
dc.identifier.issn.none.fl_str_mv |
1365-313X |
identifier_str_mv |
1365-313X |
url |
https://doi.org/10.1111/tpj.13889 http://www.locus.ufv.br/handle/123456789/24494 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartofseries.pt-BR.fl_str_mv |
v. 94, n. 4, p. 583-594, maio 2018 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
The Plant Journal |
publisher.none.fl_str_mv |
The Plant Journal |
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reponame:LOCUS Repositório Institucional da UFV instname:Universidade Federal de Viçosa (UFV) instacron:UFV |
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Universidade Federal de Viçosa (UFV) |
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UFV |
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UFV |
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LOCUS Repositório Institucional da UFV |
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LOCUS Repositório Institucional da UFV |
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https://locus.ufv.br//bitstream/123456789/24494/1/artigo.pdf https://locus.ufv.br//bitstream/123456789/24494/2/license.txt |
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