Identificação e validação de seqüências expressas em tecido muscular de suínos

• Autor(a) principal: Peixoto, Jane de Oliveira
• Data de Publicação: 2007
• Tipo de documento: Tese
• Idioma: por
• Título da fonte: LOCUS Repositório Institucional da UFV
• Texto Completo: http://locus.ufv.br/handle/123456789/1896
• topic: Expressão gênica; ESTs; Suíno; Gene expression; ESTs; Swine; CNPQ::CIENCIAS AGRARIAS::ZOOTECNIA::GENETICA E MELHORAMENTO DOS ANIMAIS DOMESTICOS

The study of expressed sequences has great importance to know and understand the genetic architecture that control quantitative traits. In this way, in this study it was produced expressed sequence tags (ESTs) in muscular tissue of pigs from Large White and Piau breeds, these ESTs were compared with public data bases and differential expressed ESTs were identified between both libraries. With this objective it was generated two cDNA libraries from semimenbranosus muscle of adult (180 days) Large White and Piau pigs. In both libraries, 4,123 ESTs have been sequenced from 5 and 3 ends. After quality analysis, 1030 sequences were considered valid for the further analysis, according to established parameters. From this total, 675 ESTs were originated from Large White library and 355 ESTs were originated from Piau library. The analyzed ESTs presented average length of 494.76 pb. Cluster analysis by CAP3 software indicates that a total of 130 sequences (11.80%) was assembled in contigs and 900 ESTs (88.20%) represented unique sequences in this collection. The sequences were compared with nucleotide and protein public data bases, resulting in 333 homologues sequences (31.54%) and 697 (68.46%) sequences that did not present homology. A total of 255 ESTs (24.30%) was submitted to genomic annotation according to Gene Ontology Consortium. In this way, the ESTs were classified according to their molecular function, process and component. Differential expressed sequences were identified between both cDNA libraries. Most of the expressed sequences represent genes not previously described in muscle tissue of pigs.