Distribution of infectious bronchitis virus strains in different organs and evidence of vertical transmission in natural infection

Detalhes bibliográficos
Autor(a) principal: Pereira, Claiton Gonçalves
Data de Publicação: 2016
Outros Autores: Saraiva, Giuliana Loreto, Vidigal, Pedro Marcus Pereira, Fietto, Juliana Lopes Rangel, Bressan, Gustavo Costa, Moreira, Maria Aparecida Scatamburlo, Almeida, Márcia Rogéria de, Silva Júnior, Abelardo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: LOCUS Repositório Institucional da UFV
Texto Completo: https://doi.org/10.1007/s00705-016-3030-5
http://www.locus.ufv.br/handle/123456789/19584
Resumo: On the basis of partial sequencing of the infectious bronchitis virus (IBV) S1 gene, this study investigated the molecular diversity of the virus in two life periods of a batch of breeding hens at the field level. The chicks were vaccinated against IBV on the second day of life with the vaccine Ma5, but at the age of 18 days, they exhibited clinical signs and macroscopic lesions compatible with avian infectious bronchitis (IB). In the clinical disease stage, the Ma5 vaccine strain was detected in the trachea, lungs, and small intestine of the chicks, while IBV variants were detected in the bursa of Fabricius and kidneys. Subsequently, new samples were collected from the same batch at the end of the production cycle. In this phase, the Ma5 vaccine strain was detected in the kidneys, small intestine, and oviduct of the hens. However, a previously unidentified IBV variant was found in the cecal tonsils. Additionally, a fragment of viral RNA with that was completely identical to the corresponding region of the Ma5 vaccine was detected in the allantoic fluid of viable embryos from the hens under study after 18 days of incubation. These findings suggest that, in addition to the Ma5 vaccine, other strains of IBV variants can coexist, seeming to establish a chronic infection in the chickens, and that they can potentially be transmitted vertically. These results may assist in immunoprophylaxis control programs against IBV
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spelling Pereira, Claiton GonçalvesSaraiva, Giuliana LoretoVidigal, Pedro Marcus PereiraFietto, Juliana Lopes RangelBressan, Gustavo CostaMoreira, Maria Aparecida ScatamburloAlmeida, Márcia Rogéria deSilva Júnior, Abelardo2018-05-16T10:46:19Z2018-05-16T10:46:19Z2016-09-0114328798https://doi.org/10.1007/s00705-016-3030-5http://www.locus.ufv.br/handle/123456789/19584On the basis of partial sequencing of the infectious bronchitis virus (IBV) S1 gene, this study investigated the molecular diversity of the virus in two life periods of a batch of breeding hens at the field level. The chicks were vaccinated against IBV on the second day of life with the vaccine Ma5, but at the age of 18 days, they exhibited clinical signs and macroscopic lesions compatible with avian infectious bronchitis (IB). In the clinical disease stage, the Ma5 vaccine strain was detected in the trachea, lungs, and small intestine of the chicks, while IBV variants were detected in the bursa of Fabricius and kidneys. Subsequently, new samples were collected from the same batch at the end of the production cycle. In this phase, the Ma5 vaccine strain was detected in the kidneys, small intestine, and oviduct of the hens. However, a previously unidentified IBV variant was found in the cecal tonsils. Additionally, a fragment of viral RNA with that was completely identical to the corresponding region of the Ma5 vaccine was detected in the allantoic fluid of viable embryos from the hens under study after 18 days of incubation. These findings suggest that, in addition to the Ma5 vaccine, other strains of IBV variants can coexist, seeming to establish a chronic infection in the chickens, and that they can potentially be transmitted vertically. These results may assist in immunoprophylaxis control programs against IBVengArchives of Virologyv. 161, Issue 12, p. 3355–3363, December 2016Springer-Verlag Wieninfo:eu-repo/semantics/openAccessVertical transmissionInfectious bronchitis virusInfectious bronchitis virus strainCecal tonsilInfectious bronchitis virus infectionDistribution of infectious bronchitis virus strains in different organs and evidence of vertical transmission in natural infectioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALartigo.pdfartigo.pdfTexto completoapplication/pdf564061https://locus.ufv.br//bitstream/123456789/19584/1/artigo.pdf869218d8f92fc70f86c9e39c4549fc55MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://locus.ufv.br//bitstream/123456789/19584/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52THUMBNAILartigo.pdf.jpgartigo.pdf.jpgIM Thumbnailimage/jpeg5161https://locus.ufv.br//bitstream/123456789/19584/3/artigo.pdf.jpg68ab6bc1f7794cce61cdb66b9f5d072fMD53123456789/195842018-05-16 23:00:45.991oai:locus.ufv.br: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Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452018-05-17T02:00:45LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false
dc.title.en.fl_str_mv Distribution of infectious bronchitis virus strains in different organs and evidence of vertical transmission in natural infection
title Distribution of infectious bronchitis virus strains in different organs and evidence of vertical transmission in natural infection
spellingShingle Distribution of infectious bronchitis virus strains in different organs and evidence of vertical transmission in natural infection
Pereira, Claiton Gonçalves
Vertical transmission
Infectious bronchitis virus
Infectious bronchitis virus strain
Cecal tonsil
Infectious bronchitis virus infection
title_short Distribution of infectious bronchitis virus strains in different organs and evidence of vertical transmission in natural infection
title_full Distribution of infectious bronchitis virus strains in different organs and evidence of vertical transmission in natural infection
title_fullStr Distribution of infectious bronchitis virus strains in different organs and evidence of vertical transmission in natural infection
title_full_unstemmed Distribution of infectious bronchitis virus strains in different organs and evidence of vertical transmission in natural infection
title_sort Distribution of infectious bronchitis virus strains in different organs and evidence of vertical transmission in natural infection
author Pereira, Claiton Gonçalves
author_facet Pereira, Claiton Gonçalves
Saraiva, Giuliana Loreto
Vidigal, Pedro Marcus Pereira
Fietto, Juliana Lopes Rangel
Bressan, Gustavo Costa
Moreira, Maria Aparecida Scatamburlo
Almeida, Márcia Rogéria de
Silva Júnior, Abelardo
author_role author
author2 Saraiva, Giuliana Loreto
Vidigal, Pedro Marcus Pereira
Fietto, Juliana Lopes Rangel
Bressan, Gustavo Costa
Moreira, Maria Aparecida Scatamburlo
Almeida, Márcia Rogéria de
Silva Júnior, Abelardo
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Pereira, Claiton Gonçalves
Saraiva, Giuliana Loreto
Vidigal, Pedro Marcus Pereira
Fietto, Juliana Lopes Rangel
Bressan, Gustavo Costa
Moreira, Maria Aparecida Scatamburlo
Almeida, Márcia Rogéria de
Silva Júnior, Abelardo
dc.subject.pt-BR.fl_str_mv Vertical transmission
Infectious bronchitis virus
Infectious bronchitis virus strain
Cecal tonsil
Infectious bronchitis virus infection
topic Vertical transmission
Infectious bronchitis virus
Infectious bronchitis virus strain
Cecal tonsil
Infectious bronchitis virus infection
description On the basis of partial sequencing of the infectious bronchitis virus (IBV) S1 gene, this study investigated the molecular diversity of the virus in two life periods of a batch of breeding hens at the field level. The chicks were vaccinated against IBV on the second day of life with the vaccine Ma5, but at the age of 18 days, they exhibited clinical signs and macroscopic lesions compatible with avian infectious bronchitis (IB). In the clinical disease stage, the Ma5 vaccine strain was detected in the trachea, lungs, and small intestine of the chicks, while IBV variants were detected in the bursa of Fabricius and kidneys. Subsequently, new samples were collected from the same batch at the end of the production cycle. In this phase, the Ma5 vaccine strain was detected in the kidneys, small intestine, and oviduct of the hens. However, a previously unidentified IBV variant was found in the cecal tonsils. Additionally, a fragment of viral RNA with that was completely identical to the corresponding region of the Ma5 vaccine was detected in the allantoic fluid of viable embryos from the hens under study after 18 days of incubation. These findings suggest that, in addition to the Ma5 vaccine, other strains of IBV variants can coexist, seeming to establish a chronic infection in the chickens, and that they can potentially be transmitted vertically. These results may assist in immunoprophylaxis control programs against IBV
publishDate 2016
dc.date.issued.fl_str_mv 2016-09-01
dc.date.accessioned.fl_str_mv 2018-05-16T10:46:19Z
dc.date.available.fl_str_mv 2018-05-16T10:46:19Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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dc.identifier.uri.fl_str_mv https://doi.org/10.1007/s00705-016-3030-5
http://www.locus.ufv.br/handle/123456789/19584
dc.identifier.issn.none.fl_str_mv 14328798
identifier_str_mv 14328798
url https://doi.org/10.1007/s00705-016-3030-5
http://www.locus.ufv.br/handle/123456789/19584
dc.language.iso.fl_str_mv eng
language eng
dc.relation.ispartofseries.pt-BR.fl_str_mv v. 161, Issue 12, p. 3355–3363, December 2016
dc.rights.driver.fl_str_mv Springer-Verlag Wien
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eu_rights_str_mv openAccess
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dc.publisher.none.fl_str_mv Archives of Virology
publisher.none.fl_str_mv Archives of Virology
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