Diversidade de fungos endofíticos de folhas de soja (Glycine max) cultivada em Viçosa – MG

• Autor(a) principal: Leite, Tiago de Souza
• Data de Publicação: 2010
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: LOCUS Repositório Institucional da UFV
• Texto Completo: http://locus.ufv.br/handle/123456789/5329
• topic: Fragmentação do tecido; Cultivo por extinção; Região ITS; Tissue fragmentation; Extinction cultivation; ITS region; CNPQ::CIENCIAS BIOLOGICAS::GENETICA

Soybean is one of the most important crops used in human and animals food. Fungal endophytes are microorganisms that live inside the plant tissue at some stage of their life cycle without causing apparent disease to the host. Many studies have shown the use of fungal endophytes as agents of biological control of pests and plants diseases, and to improve the plant growth and induce systemic resistance of plants. The goal of this work was to isolate and identify the endophytic fungi from leaves of soybean cultivars Monsoy and Conquista using two method of isolation (tissue fragmentation and extinction cultivation). Furthermore, was determining the species richness and the diversity of fungi and compared between cultivars. 188 morphologically distinct isolates were obtained representing 52 taxa identified by sequencing the ITS region of rDNA. The phylum Ascomycota was the dominant represented by 99 and 96 % of the isolates to Monsoy and Conquista cultivars respectively. Species richness of cultivars Monsoy (31 species) and Conquista (37 species) was greater when both isolation method were used. Diversity of endophytic fungi was similar for both cultivars using the same isolation methods. The nucleotide sequences of the ITS region was used for phylogenetic analysis and allowed the grouping of fungal isolates according to their respective Order (when present), Class and Phylum. This is one of the first studies using the extinction culturing method for endophytic fungal isolation in soybean.