Molecular detection ofLeishmaniaspp. in road-killed wild mammals in the Central Western area of the State of São Paulo, Brazil
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | The Journal of venomous animals and toxins including tropical diseases (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200331 |
Resumo: | Background Road-killed wild animals have been classified as sentinels for detecting such zoonotic pathogens asLeishmaniaspp., offering new opportunities for epidemiological studies of this infection.Methods This study aimed to evaluate the presence ofLeishmaniaspp. andLeishmania chagasiDNA by PCR in tissue samples (lung, liver, spleen, kidney, heart, mesenteric lymph node and adrenal gland) from 70 road-killed wild animals.Results DNA was detected in tissues of oneCavia aperea(Brazilian guinea pig), fiveCerdocyon thous(crab-eating fox), oneDasypus septemcinctus(seven-banded armadillo), twoDidelphis albiventris(white-eared opossum), oneHydrochoerus hydrochoeris(capybara), twoMyrmecophaga tridactyla(giant anteater), oneProcyon cancrivorus(crab-eating raccoon), twoSphiggurus spinosus(porcupine) and oneTamandua tetradactyla(lesser anteater) from different locations in the Central Western part of São Paulo state. TheLeishmania chagasiDNA were confirmed in mesenteric lymph node of oneCerdocyon thous. Results indicated common infection in wild animals.Conclusions The approach employed herein proved useful for detecting the environmental occurrence ofLeishmaniaspp. andL. chagasi, as well as determining natural wild reservoirs and contributing to understand the host-parasite interaction. |
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The Journal of venomous animals and toxins including tropical diseases (Online) |
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Molecular detection ofLeishmaniaspp. in road-killed wild mammals in the Central Western area of the State of São Paulo, BrazilRoad-killed animalLeishmaniasppLeishmania chagasiPCRZoonosisBackground Road-killed wild animals have been classified as sentinels for detecting such zoonotic pathogens asLeishmaniaspp., offering new opportunities for epidemiological studies of this infection.Methods This study aimed to evaluate the presence ofLeishmaniaspp. andLeishmania chagasiDNA by PCR in tissue samples (lung, liver, spleen, kidney, heart, mesenteric lymph node and adrenal gland) from 70 road-killed wild animals.Results DNA was detected in tissues of oneCavia aperea(Brazilian guinea pig), fiveCerdocyon thous(crab-eating fox), oneDasypus septemcinctus(seven-banded armadillo), twoDidelphis albiventris(white-eared opossum), oneHydrochoerus hydrochoeris(capybara), twoMyrmecophaga tridactyla(giant anteater), oneProcyon cancrivorus(crab-eating raccoon), twoSphiggurus spinosus(porcupine) and oneTamandua tetradactyla(lesser anteater) from different locations in the Central Western part of São Paulo state. TheLeishmania chagasiDNA were confirmed in mesenteric lymph node of oneCerdocyon thous. Results indicated common infection in wild animals.Conclusions The approach employed herein proved useful for detecting the environmental occurrence ofLeishmaniaspp. andL. chagasi, as well as determining natural wild reservoirs and contributing to understand the host-parasite interaction.Centro de Estudos de Venenos e Animais Peçonhentos (CEVAP/UNESP)2014-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200331Journal of Venomous Animals and Toxins including Tropical Diseases v.20 2014reponame:The Journal of venomous animals and toxins including tropical diseases (Online)instname:Universidade Estadual Paulista (UNESP)instacron:UNESP10.1186/1678-9199-20-27info:eu-repo/semantics/openAccessRichini-Pereira,Virginia BodelãoMarson,Pamela MerloHayasaka,Enio YoshinoriVictoria,CassianoSilva,Rodrigo Costa daLangoni,Hélioeng2018-08-17T00:00:00Zoai:scielo:S1678-91992014000200331Revistahttp://www.scielo.br/jvatitdPUBhttps://old.scielo.br/oai/scielo-oai.php||editorial@jvat.org.br1678-91991678-9180opendoar:2018-08-17T00:00The Journal of venomous animals and toxins including tropical diseases (Online) - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Molecular detection ofLeishmaniaspp. in road-killed wild mammals in the Central Western area of the State of São Paulo, Brazil |
title |
Molecular detection ofLeishmaniaspp. in road-killed wild mammals in the Central Western area of the State of São Paulo, Brazil |
spellingShingle |
Molecular detection ofLeishmaniaspp. in road-killed wild mammals in the Central Western area of the State of São Paulo, Brazil Richini-Pereira,Virginia Bodelão Road-killed animal Leishmaniaspp Leishmania chagasi PCR Zoonosis |
title_short |
Molecular detection ofLeishmaniaspp. in road-killed wild mammals in the Central Western area of the State of São Paulo, Brazil |
title_full |
Molecular detection ofLeishmaniaspp. in road-killed wild mammals in the Central Western area of the State of São Paulo, Brazil |
title_fullStr |
Molecular detection ofLeishmaniaspp. in road-killed wild mammals in the Central Western area of the State of São Paulo, Brazil |
title_full_unstemmed |
Molecular detection ofLeishmaniaspp. in road-killed wild mammals in the Central Western area of the State of São Paulo, Brazil |
title_sort |
Molecular detection ofLeishmaniaspp. in road-killed wild mammals in the Central Western area of the State of São Paulo, Brazil |
author |
Richini-Pereira,Virginia Bodelão |
author_facet |
Richini-Pereira,Virginia Bodelão Marson,Pamela Merlo Hayasaka,Enio Yoshinori Victoria,Cassiano Silva,Rodrigo Costa da Langoni,Hélio |
author_role |
author |
author2 |
Marson,Pamela Merlo Hayasaka,Enio Yoshinori Victoria,Cassiano Silva,Rodrigo Costa da Langoni,Hélio |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Richini-Pereira,Virginia Bodelão Marson,Pamela Merlo Hayasaka,Enio Yoshinori Victoria,Cassiano Silva,Rodrigo Costa da Langoni,Hélio |
dc.subject.por.fl_str_mv |
Road-killed animal Leishmaniaspp Leishmania chagasi PCR Zoonosis |
topic |
Road-killed animal Leishmaniaspp Leishmania chagasi PCR Zoonosis |
description |
Background Road-killed wild animals have been classified as sentinels for detecting such zoonotic pathogens asLeishmaniaspp., offering new opportunities for epidemiological studies of this infection.Methods This study aimed to evaluate the presence ofLeishmaniaspp. andLeishmania chagasiDNA by PCR in tissue samples (lung, liver, spleen, kidney, heart, mesenteric lymph node and adrenal gland) from 70 road-killed wild animals.Results DNA was detected in tissues of oneCavia aperea(Brazilian guinea pig), fiveCerdocyon thous(crab-eating fox), oneDasypus septemcinctus(seven-banded armadillo), twoDidelphis albiventris(white-eared opossum), oneHydrochoerus hydrochoeris(capybara), twoMyrmecophaga tridactyla(giant anteater), oneProcyon cancrivorus(crab-eating raccoon), twoSphiggurus spinosus(porcupine) and oneTamandua tetradactyla(lesser anteater) from different locations in the Central Western part of São Paulo state. TheLeishmania chagasiDNA were confirmed in mesenteric lymph node of oneCerdocyon thous. Results indicated common infection in wild animals.Conclusions The approach employed herein proved useful for detecting the environmental occurrence ofLeishmaniaspp. andL. chagasi, as well as determining natural wild reservoirs and contributing to understand the host-parasite interaction. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200331 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200331 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1186/1678-9199-20-27 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Centro de Estudos de Venenos e Animais Peçonhentos (CEVAP/UNESP) |
publisher.none.fl_str_mv |
Centro de Estudos de Venenos e Animais Peçonhentos (CEVAP/UNESP) |
dc.source.none.fl_str_mv |
Journal of Venomous Animals and Toxins including Tropical Diseases v.20 2014 reponame:The Journal of venomous animals and toxins including tropical diseases (Online) instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
The Journal of venomous animals and toxins including tropical diseases (Online) |
collection |
The Journal of venomous animals and toxins including tropical diseases (Online) |
repository.name.fl_str_mv |
The Journal of venomous animals and toxins including tropical diseases (Online) - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
||editorial@jvat.org.br |
_version_ |
1748958539626315776 |