Laser phototherapy induces angiogenesis in the periodontal tissue after delayed tooth replantation in rats
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Publication Date: | 2018 |
Other Authors: | , , , , , |
Format: | Article |
Language: | eng |
Source: | Repositório Institucional da UNESP |
Download full: | http://dx.doi.org/10.4317/jced.54499 http://hdl.handle.net/11449/221051 |
Summary: | Background: Laser phototherapy (LPT) has been suggested as a new therapeutic tool to improve the repair of replanted teeth. However, its effects and mechanism of action are not yet completely understood. Objectives: This study evaluated histologically the effect of laser phototherapy (LPT) λ808 and λ660 nm on angiogenesis in the periodontal tissue of replanted teeth in rats. Material and Methods: Twenty maxillary right incisors were extracted from twenty Wistar rats and randomly assigned to four groups (n = 5): PN - teeth were stored in paper napkin for 45 min; WM - teeth were immersed in 20 ml of UHT whole cow milk for 45 min; PNL and WML - teeth received the same treatment described for PN and WM, respectively, plus LPT at λ808 and λ660 nm. All root canals were prepared and filled with calcium hydroxide paste. The animals were euthanized 15 days after tooth replantation and angiogenesis was scored by blood vessel counting in the area of periodontal ligament and alveolar bone, using the ImageJ software. Data were analyzed statistically by ANOVA and Tukey's test (α = 5%). Results: LPT at λ808 and λ660 nm caused significant increased angiogenesis on irradiated groups (PNL and WML) when compared to the non-irradiated groups (PN and WM) (p < 0.05). There was no statistically significant difference between PN and WM as well as between PNL and WML (p > 0.05). Conclusions: LPT is capable of stimulating angiogenesis in vivo in the periodontal tissue of replanted teeth. |
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Laser phototherapy induces angiogenesis in the periodontal tissue after delayed tooth replantation in ratsAngiogenesisLasersTooth avulsionTooth replantationWound healingBackground: Laser phototherapy (LPT) has been suggested as a new therapeutic tool to improve the repair of replanted teeth. However, its effects and mechanism of action are not yet completely understood. Objectives: This study evaluated histologically the effect of laser phototherapy (LPT) λ808 and λ660 nm on angiogenesis in the periodontal tissue of replanted teeth in rats. Material and Methods: Twenty maxillary right incisors were extracted from twenty Wistar rats and randomly assigned to four groups (n = 5): PN - teeth were stored in paper napkin for 45 min; WM - teeth were immersed in 20 ml of UHT whole cow milk for 45 min; PNL and WML - teeth received the same treatment described for PN and WM, respectively, plus LPT at λ808 and λ660 nm. All root canals were prepared and filled with calcium hydroxide paste. The animals were euthanized 15 days after tooth replantation and angiogenesis was scored by blood vessel counting in the area of periodontal ligament and alveolar bone, using the ImageJ software. Data were analyzed statistically by ANOVA and Tukey's test (α = 5%). Results: LPT at λ808 and λ660 nm caused significant increased angiogenesis on irradiated groups (PNL and WML) when compared to the non-irradiated groups (PN and WM) (p < 0.05). There was no statistically significant difference between PN and WM as well as between PNL and WML (p > 0.05). Conclusions: LPT is capable of stimulating angiogenesis in vivo in the periodontal tissue of replanted teeth.Department of Restorative Dentistry Endodontic Division Institute of Science and Technology São Paulo State UniversityDepartment of Dentistry Federal University of SergipeDepartment of Dentistry Tiradentes UniversityDepartment of Oral Pathology Institute of Science and Technology São Paulo State UniversityDepartment of Restorative Dentistry Endodontic Division Institute of Science and Technology São Paulo State UniversityDepartment of Oral Pathology Institute of Science and Technology São Paulo State UniversityUniversidade Estadual Paulista (UNESP)Universidade Federal de Sergipe (UFS)Tiradentes UniversityMatos, Felipe-de Souza [UNESP]Godolphim, Fernanda-de JesusAlbuquerque-Júnior, Ricardo-Luiz-CavalcantiParanhos, Luiz-RenatoRode, Sigmar-de Mello [UNESP]Carvalho, Cláudio-Antonio-Talge [UNESP]Ribeiro, Maria-Amália-Gonzaga2022-04-28T19:08:50Z2022-04-28T19:08:50Z2018-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlee335-e340http://dx.doi.org/10.4317/jced.54499Journal of Clinical and Experimental Dentistry, v. 10, n. 4, p. e335-e340, 2018.1989-5488http://hdl.handle.net/11449/22105110.4317/jced.544992-s2.0-85044729828Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Clinical and Experimental Dentistryinfo:eu-repo/semantics/openAccess2022-04-28T19:08:50Zoai:repositorio.unesp.br:11449/221051Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T18:31:46.742022Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Laser phototherapy induces angiogenesis in the periodontal tissue after delayed tooth replantation in rats |
title |
Laser phototherapy induces angiogenesis in the periodontal tissue after delayed tooth replantation in rats |
spellingShingle |
Laser phototherapy induces angiogenesis in the periodontal tissue after delayed tooth replantation in rats Matos, Felipe-de Souza [UNESP] Angiogenesis Lasers Tooth avulsion Tooth replantation Wound healing |
title_short |
Laser phototherapy induces angiogenesis in the periodontal tissue after delayed tooth replantation in rats |
title_full |
Laser phototherapy induces angiogenesis in the periodontal tissue after delayed tooth replantation in rats |
title_fullStr |
Laser phototherapy induces angiogenesis in the periodontal tissue after delayed tooth replantation in rats |
title_full_unstemmed |
Laser phototherapy induces angiogenesis in the periodontal tissue after delayed tooth replantation in rats |
title_sort |
Laser phototherapy induces angiogenesis in the periodontal tissue after delayed tooth replantation in rats |
author |
Matos, Felipe-de Souza [UNESP] |
author_facet |
Matos, Felipe-de Souza [UNESP] Godolphim, Fernanda-de Jesus Albuquerque-Júnior, Ricardo-Luiz-Cavalcanti Paranhos, Luiz-Renato Rode, Sigmar-de Mello [UNESP] Carvalho, Cláudio-Antonio-Talge [UNESP] Ribeiro, Maria-Amália-Gonzaga |
author_role |
author |
author2 |
Godolphim, Fernanda-de Jesus Albuquerque-Júnior, Ricardo-Luiz-Cavalcanti Paranhos, Luiz-Renato Rode, Sigmar-de Mello [UNESP] Carvalho, Cláudio-Antonio-Talge [UNESP] Ribeiro, Maria-Amália-Gonzaga |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Universidade Federal de Sergipe (UFS) Tiradentes University |
dc.contributor.author.fl_str_mv |
Matos, Felipe-de Souza [UNESP] Godolphim, Fernanda-de Jesus Albuquerque-Júnior, Ricardo-Luiz-Cavalcanti Paranhos, Luiz-Renato Rode, Sigmar-de Mello [UNESP] Carvalho, Cláudio-Antonio-Talge [UNESP] Ribeiro, Maria-Amália-Gonzaga |
dc.subject.por.fl_str_mv |
Angiogenesis Lasers Tooth avulsion Tooth replantation Wound healing |
topic |
Angiogenesis Lasers Tooth avulsion Tooth replantation Wound healing |
description |
Background: Laser phototherapy (LPT) has been suggested as a new therapeutic tool to improve the repair of replanted teeth. However, its effects and mechanism of action are not yet completely understood. Objectives: This study evaluated histologically the effect of laser phototherapy (LPT) λ808 and λ660 nm on angiogenesis in the periodontal tissue of replanted teeth in rats. Material and Methods: Twenty maxillary right incisors were extracted from twenty Wistar rats and randomly assigned to four groups (n = 5): PN - teeth were stored in paper napkin for 45 min; WM - teeth were immersed in 20 ml of UHT whole cow milk for 45 min; PNL and WML - teeth received the same treatment described for PN and WM, respectively, plus LPT at λ808 and λ660 nm. All root canals were prepared and filled with calcium hydroxide paste. The animals were euthanized 15 days after tooth replantation and angiogenesis was scored by blood vessel counting in the area of periodontal ligament and alveolar bone, using the ImageJ software. Data were analyzed statistically by ANOVA and Tukey's test (α = 5%). Results: LPT at λ808 and λ660 nm caused significant increased angiogenesis on irradiated groups (PNL and WML) when compared to the non-irradiated groups (PN and WM) (p < 0.05). There was no statistically significant difference between PN and WM as well as between PNL and WML (p > 0.05). Conclusions: LPT is capable of stimulating angiogenesis in vivo in the periodontal tissue of replanted teeth. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-04-01 2022-04-28T19:08:50Z 2022-04-28T19:08:50Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.4317/jced.54499 Journal of Clinical and Experimental Dentistry, v. 10, n. 4, p. e335-e340, 2018. 1989-5488 http://hdl.handle.net/11449/221051 10.4317/jced.54499 2-s2.0-85044729828 |
url |
http://dx.doi.org/10.4317/jced.54499 http://hdl.handle.net/11449/221051 |
identifier_str_mv |
Journal of Clinical and Experimental Dentistry, v. 10, n. 4, p. e335-e340, 2018. 1989-5488 10.4317/jced.54499 2-s2.0-85044729828 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Clinical and Experimental Dentistry |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
e335-e340 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808128943233957888 |