Laser phototherapy induces angiogenesis in the periodontal tissue after delayed tooth replantation in rats

Bibliographic Details
Main Author: Matos, Felipe-de Souza [UNESP]
Publication Date: 2018
Other Authors: Godolphim, Fernanda-de Jesus, Albuquerque-Júnior, Ricardo-Luiz-Cavalcanti, Paranhos, Luiz-Renato, Rode, Sigmar-de Mello [UNESP], Carvalho, Cláudio-Antonio-Talge [UNESP], Ribeiro, Maria-Amália-Gonzaga
Format: Article
Language: eng
Source: Repositório Institucional da UNESP
Download full: http://dx.doi.org/10.4317/jced.54499
http://hdl.handle.net/11449/221051
Summary: Background: Laser phototherapy (LPT) has been suggested as a new therapeutic tool to improve the repair of replanted teeth. However, its effects and mechanism of action are not yet completely understood. Objectives: This study evaluated histologically the effect of laser phototherapy (LPT) λ808 and λ660 nm on angiogenesis in the periodontal tissue of replanted teeth in rats. Material and Methods: Twenty maxillary right incisors were extracted from twenty Wistar rats and randomly assigned to four groups (n = 5): PN - teeth were stored in paper napkin for 45 min; WM - teeth were immersed in 20 ml of UHT whole cow milk for 45 min; PNL and WML - teeth received the same treatment described for PN and WM, respectively, plus LPT at λ808 and λ660 nm. All root canals were prepared and filled with calcium hydroxide paste. The animals were euthanized 15 days after tooth replantation and angiogenesis was scored by blood vessel counting in the area of periodontal ligament and alveolar bone, using the ImageJ software. Data were analyzed statistically by ANOVA and Tukey's test (α = 5%). Results: LPT at λ808 and λ660 nm caused significant increased angiogenesis on irradiated groups (PNL and WML) when compared to the non-irradiated groups (PN and WM) (p < 0.05). There was no statistically significant difference between PN and WM as well as between PNL and WML (p > 0.05). Conclusions: LPT is capable of stimulating angiogenesis in vivo in the periodontal tissue of replanted teeth.
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spelling Laser phototherapy induces angiogenesis in the periodontal tissue after delayed tooth replantation in ratsAngiogenesisLasersTooth avulsionTooth replantationWound healingBackground: Laser phototherapy (LPT) has been suggested as a new therapeutic tool to improve the repair of replanted teeth. However, its effects and mechanism of action are not yet completely understood. Objectives: This study evaluated histologically the effect of laser phototherapy (LPT) λ808 and λ660 nm on angiogenesis in the periodontal tissue of replanted teeth in rats. Material and Methods: Twenty maxillary right incisors were extracted from twenty Wistar rats and randomly assigned to four groups (n = 5): PN - teeth were stored in paper napkin for 45 min; WM - teeth were immersed in 20 ml of UHT whole cow milk for 45 min; PNL and WML - teeth received the same treatment described for PN and WM, respectively, plus LPT at λ808 and λ660 nm. All root canals were prepared and filled with calcium hydroxide paste. The animals were euthanized 15 days after tooth replantation and angiogenesis was scored by blood vessel counting in the area of periodontal ligament and alveolar bone, using the ImageJ software. Data were analyzed statistically by ANOVA and Tukey's test (α = 5%). Results: LPT at λ808 and λ660 nm caused significant increased angiogenesis on irradiated groups (PNL and WML) when compared to the non-irradiated groups (PN and WM) (p < 0.05). There was no statistically significant difference between PN and WM as well as between PNL and WML (p > 0.05). Conclusions: LPT is capable of stimulating angiogenesis in vivo in the periodontal tissue of replanted teeth.Department of Restorative Dentistry Endodontic Division Institute of Science and Technology São Paulo State UniversityDepartment of Dentistry Federal University of SergipeDepartment of Dentistry Tiradentes UniversityDepartment of Oral Pathology Institute of Science and Technology São Paulo State UniversityDepartment of Restorative Dentistry Endodontic Division Institute of Science and Technology São Paulo State UniversityDepartment of Oral Pathology Institute of Science and Technology São Paulo State UniversityUniversidade Estadual Paulista (UNESP)Universidade Federal de Sergipe (UFS)Tiradentes UniversityMatos, Felipe-de Souza [UNESP]Godolphim, Fernanda-de JesusAlbuquerque-Júnior, Ricardo-Luiz-CavalcantiParanhos, Luiz-RenatoRode, Sigmar-de Mello [UNESP]Carvalho, Cláudio-Antonio-Talge [UNESP]Ribeiro, Maria-Amália-Gonzaga2022-04-28T19:08:50Z2022-04-28T19:08:50Z2018-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlee335-e340http://dx.doi.org/10.4317/jced.54499Journal of Clinical and Experimental Dentistry, v. 10, n. 4, p. e335-e340, 2018.1989-5488http://hdl.handle.net/11449/22105110.4317/jced.544992-s2.0-85044729828Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Clinical and Experimental Dentistryinfo:eu-repo/semantics/openAccess2022-04-28T19:08:50Zoai:repositorio.unesp.br:11449/221051Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T18:31:46.742022Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Laser phototherapy induces angiogenesis in the periodontal tissue after delayed tooth replantation in rats
title Laser phototherapy induces angiogenesis in the periodontal tissue after delayed tooth replantation in rats
spellingShingle Laser phototherapy induces angiogenesis in the periodontal tissue after delayed tooth replantation in rats
Matos, Felipe-de Souza [UNESP]
Angiogenesis
Lasers
Tooth avulsion
Tooth replantation
Wound healing
title_short Laser phototherapy induces angiogenesis in the periodontal tissue after delayed tooth replantation in rats
title_full Laser phototherapy induces angiogenesis in the periodontal tissue after delayed tooth replantation in rats
title_fullStr Laser phototherapy induces angiogenesis in the periodontal tissue after delayed tooth replantation in rats
title_full_unstemmed Laser phototherapy induces angiogenesis in the periodontal tissue after delayed tooth replantation in rats
title_sort Laser phototherapy induces angiogenesis in the periodontal tissue after delayed tooth replantation in rats
author Matos, Felipe-de Souza [UNESP]
author_facet Matos, Felipe-de Souza [UNESP]
Godolphim, Fernanda-de Jesus
Albuquerque-Júnior, Ricardo-Luiz-Cavalcanti
Paranhos, Luiz-Renato
Rode, Sigmar-de Mello [UNESP]
Carvalho, Cláudio-Antonio-Talge [UNESP]
Ribeiro, Maria-Amália-Gonzaga
author_role author
author2 Godolphim, Fernanda-de Jesus
Albuquerque-Júnior, Ricardo-Luiz-Cavalcanti
Paranhos, Luiz-Renato
Rode, Sigmar-de Mello [UNESP]
Carvalho, Cláudio-Antonio-Talge [UNESP]
Ribeiro, Maria-Amália-Gonzaga
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
Universidade Federal de Sergipe (UFS)
Tiradentes University
dc.contributor.author.fl_str_mv Matos, Felipe-de Souza [UNESP]
Godolphim, Fernanda-de Jesus
Albuquerque-Júnior, Ricardo-Luiz-Cavalcanti
Paranhos, Luiz-Renato
Rode, Sigmar-de Mello [UNESP]
Carvalho, Cláudio-Antonio-Talge [UNESP]
Ribeiro, Maria-Amália-Gonzaga
dc.subject.por.fl_str_mv Angiogenesis
Lasers
Tooth avulsion
Tooth replantation
Wound healing
topic Angiogenesis
Lasers
Tooth avulsion
Tooth replantation
Wound healing
description Background: Laser phototherapy (LPT) has been suggested as a new therapeutic tool to improve the repair of replanted teeth. However, its effects and mechanism of action are not yet completely understood. Objectives: This study evaluated histologically the effect of laser phototherapy (LPT) λ808 and λ660 nm on angiogenesis in the periodontal tissue of replanted teeth in rats. Material and Methods: Twenty maxillary right incisors were extracted from twenty Wistar rats and randomly assigned to four groups (n = 5): PN - teeth were stored in paper napkin for 45 min; WM - teeth were immersed in 20 ml of UHT whole cow milk for 45 min; PNL and WML - teeth received the same treatment described for PN and WM, respectively, plus LPT at λ808 and λ660 nm. All root canals were prepared and filled with calcium hydroxide paste. The animals were euthanized 15 days after tooth replantation and angiogenesis was scored by blood vessel counting in the area of periodontal ligament and alveolar bone, using the ImageJ software. Data were analyzed statistically by ANOVA and Tukey's test (α = 5%). Results: LPT at λ808 and λ660 nm caused significant increased angiogenesis on irradiated groups (PNL and WML) when compared to the non-irradiated groups (PN and WM) (p < 0.05). There was no statistically significant difference between PN and WM as well as between PNL and WML (p > 0.05). Conclusions: LPT is capable of stimulating angiogenesis in vivo in the periodontal tissue of replanted teeth.
publishDate 2018
dc.date.none.fl_str_mv 2018-04-01
2022-04-28T19:08:50Z
2022-04-28T19:08:50Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.4317/jced.54499
Journal of Clinical and Experimental Dentistry, v. 10, n. 4, p. e335-e340, 2018.
1989-5488
http://hdl.handle.net/11449/221051
10.4317/jced.54499
2-s2.0-85044729828
url http://dx.doi.org/10.4317/jced.54499
http://hdl.handle.net/11449/221051
identifier_str_mv Journal of Clinical and Experimental Dentistry, v. 10, n. 4, p. e335-e340, 2018.
1989-5488
10.4317/jced.54499
2-s2.0-85044729828
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Journal of Clinical and Experimental Dentistry
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv e335-e340
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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