Photo-responsive polymeric micelles for the light-triggered release of curcumin targeting antimicrobial activity
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3389/fmicb.2023.1132781 http://hdl.handle.net/11449/249931 |
Resumo: | Nanocarriers have been successfully used to solubilize, deliver, and increase the bioavailability of curcumin (CUR), but slow CUR release rates hinder its use as a topical photosensitizer in antimicrobial photodynamic therapy. A photo-responsive polymer (PRP) was designed for the light-triggered release of CUR with an effective light activation-dependent antimicrobial response. The characterization of the PRP was compared with non-responsive micelles comprising Pluronics™ P123 and F127. According to the findings, the PRP formed photo-responsive micelles in the nanometric scale (< 100 nm) with a lower critical micelle concentration (3.74 × 10−4 M−1, 5.8 × 10−4 M−1, and 7.2 × 10−6 M−1 for PRP, F127, P123, respectively, at 25°C) and higher entrapment efficiency of CUR (88.7, 77.2, and 72.3% for PRP, F127, and P123 micelles, respectively) than the pluronics evaluated. The PRP provided enhanced protection of CUR compared to P123 micelles, as demonstrated in fluorescence quenching studies. The light-triggered release of CUR from PRP occurred with UV light irradiation (at 355 nm and 25 mW cm−2) and a cumulative release of 88.34% of CUR within 1 h compared to 80% from pluronics after 36 h. In vitro studies showed that CUR-loaded PRP was non-toxic to mammal cell, showed inactivation of the pathogenic microorganisms Candida albicans, Pseudomonas aeruginosa, and methicillin-resistant Staphylococcus aureus, and decreased biofilm biomass when associated with blue light (455 nm, 33.84 J/cm2). The findings show that the CUR-loaded PRP micelle is a viable option for antimicrobial activity. |
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Photo-responsive polymeric micelles for the light-triggered release of curcumin targeting antimicrobial activitybacteriafungilightmicellesPhotochemotherapyNanocarriers have been successfully used to solubilize, deliver, and increase the bioavailability of curcumin (CUR), but slow CUR release rates hinder its use as a topical photosensitizer in antimicrobial photodynamic therapy. A photo-responsive polymer (PRP) was designed for the light-triggered release of CUR with an effective light activation-dependent antimicrobial response. The characterization of the PRP was compared with non-responsive micelles comprising Pluronics™ P123 and F127. According to the findings, the PRP formed photo-responsive micelles in the nanometric scale (< 100 nm) with a lower critical micelle concentration (3.74 × 10−4 M−1, 5.8 × 10−4 M−1, and 7.2 × 10−6 M−1 for PRP, F127, P123, respectively, at 25°C) and higher entrapment efficiency of CUR (88.7, 77.2, and 72.3% for PRP, F127, and P123 micelles, respectively) than the pluronics evaluated. The PRP provided enhanced protection of CUR compared to P123 micelles, as demonstrated in fluorescence quenching studies. The light-triggered release of CUR from PRP occurred with UV light irradiation (at 355 nm and 25 mW cm−2) and a cumulative release of 88.34% of CUR within 1 h compared to 80% from pluronics after 36 h. In vitro studies showed that CUR-loaded PRP was non-toxic to mammal cell, showed inactivation of the pathogenic microorganisms Candida albicans, Pseudomonas aeruginosa, and methicillin-resistant Staphylococcus aureus, and decreased biofilm biomass when associated with blue light (455 nm, 33.84 J/cm2). The findings show that the CUR-loaded PRP micelle is a viable option for antimicrobial activity.Laboratory of Applied Microbiology Department of Dental Materials and Prosthodontics School of Dentistry São Paulo State University (UNESP)Department of Chemistry Center of Nanotechnology and Tissue Engineering Photobiology and Photomedicine Research Group Faculty of Philosophy Sciences and Letters of Ribeirão Preto University of São Paulo, São PauloDepartment of Chemistry Research Nucleus of Photodynamic Therapy State University of Maringá, ParanáLaboratory of Applied Microbiology Department of Dental Materials and Prosthodontics School of Dentistry São Paulo State University (UNESP)Universidade Estadual Paulista (UNESP)Universidade de São Paulo (USP)State University of MaringáTrigo-Gutierrez, Jeffersson Krishan [UNESP]Calori, Italo Rodrigode Oliveira Bárbara, Geovana [UNESP]Pavarina, Ana Claudia [UNESP]Gonçalves, Renato SonchiniCaetano, WilkerTedesco, Antonio ClaudioMima, Ewerton Garcia de Oliveira [UNESP]2023-07-29T16:13:08Z2023-07-29T16:13:08Z2023-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3389/fmicb.2023.1132781Frontiers in Microbiology, v. 14.1664-302Xhttp://hdl.handle.net/11449/24993110.3389/fmicb.2023.11327812-s2.0-85158009632Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengFrontiers in Microbiologyinfo:eu-repo/semantics/openAccess2023-07-29T16:13:08Zoai:repositorio.unesp.br:11449/249931Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-07-29T16:13:08Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Photo-responsive polymeric micelles for the light-triggered release of curcumin targeting antimicrobial activity |
title |
Photo-responsive polymeric micelles for the light-triggered release of curcumin targeting antimicrobial activity |
spellingShingle |
Photo-responsive polymeric micelles for the light-triggered release of curcumin targeting antimicrobial activity Trigo-Gutierrez, Jeffersson Krishan [UNESP] bacteria fungi light micelles Photochemotherapy |
title_short |
Photo-responsive polymeric micelles for the light-triggered release of curcumin targeting antimicrobial activity |
title_full |
Photo-responsive polymeric micelles for the light-triggered release of curcumin targeting antimicrobial activity |
title_fullStr |
Photo-responsive polymeric micelles for the light-triggered release of curcumin targeting antimicrobial activity |
title_full_unstemmed |
Photo-responsive polymeric micelles for the light-triggered release of curcumin targeting antimicrobial activity |
title_sort |
Photo-responsive polymeric micelles for the light-triggered release of curcumin targeting antimicrobial activity |
author |
Trigo-Gutierrez, Jeffersson Krishan [UNESP] |
author_facet |
Trigo-Gutierrez, Jeffersson Krishan [UNESP] Calori, Italo Rodrigo de Oliveira Bárbara, Geovana [UNESP] Pavarina, Ana Claudia [UNESP] Gonçalves, Renato Sonchini Caetano, Wilker Tedesco, Antonio Claudio Mima, Ewerton Garcia de Oliveira [UNESP] |
author_role |
author |
author2 |
Calori, Italo Rodrigo de Oliveira Bárbara, Geovana [UNESP] Pavarina, Ana Claudia [UNESP] Gonçalves, Renato Sonchini Caetano, Wilker Tedesco, Antonio Claudio Mima, Ewerton Garcia de Oliveira [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Universidade de São Paulo (USP) State University of Maringá |
dc.contributor.author.fl_str_mv |
Trigo-Gutierrez, Jeffersson Krishan [UNESP] Calori, Italo Rodrigo de Oliveira Bárbara, Geovana [UNESP] Pavarina, Ana Claudia [UNESP] Gonçalves, Renato Sonchini Caetano, Wilker Tedesco, Antonio Claudio Mima, Ewerton Garcia de Oliveira [UNESP] |
dc.subject.por.fl_str_mv |
bacteria fungi light micelles Photochemotherapy |
topic |
bacteria fungi light micelles Photochemotherapy |
description |
Nanocarriers have been successfully used to solubilize, deliver, and increase the bioavailability of curcumin (CUR), but slow CUR release rates hinder its use as a topical photosensitizer in antimicrobial photodynamic therapy. A photo-responsive polymer (PRP) was designed for the light-triggered release of CUR with an effective light activation-dependent antimicrobial response. The characterization of the PRP was compared with non-responsive micelles comprising Pluronics™ P123 and F127. According to the findings, the PRP formed photo-responsive micelles in the nanometric scale (< 100 nm) with a lower critical micelle concentration (3.74 × 10−4 M−1, 5.8 × 10−4 M−1, and 7.2 × 10−6 M−1 for PRP, F127, P123, respectively, at 25°C) and higher entrapment efficiency of CUR (88.7, 77.2, and 72.3% for PRP, F127, and P123 micelles, respectively) than the pluronics evaluated. The PRP provided enhanced protection of CUR compared to P123 micelles, as demonstrated in fluorescence quenching studies. The light-triggered release of CUR from PRP occurred with UV light irradiation (at 355 nm and 25 mW cm−2) and a cumulative release of 88.34% of CUR within 1 h compared to 80% from pluronics after 36 h. In vitro studies showed that CUR-loaded PRP was non-toxic to mammal cell, showed inactivation of the pathogenic microorganisms Candida albicans, Pseudomonas aeruginosa, and methicillin-resistant Staphylococcus aureus, and decreased biofilm biomass when associated with blue light (455 nm, 33.84 J/cm2). The findings show that the CUR-loaded PRP micelle is a viable option for antimicrobial activity. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-07-29T16:13:08Z 2023-07-29T16:13:08Z 2023-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3389/fmicb.2023.1132781 Frontiers in Microbiology, v. 14. 1664-302X http://hdl.handle.net/11449/249931 10.3389/fmicb.2023.1132781 2-s2.0-85158009632 |
url |
http://dx.doi.org/10.3389/fmicb.2023.1132781 http://hdl.handle.net/11449/249931 |
identifier_str_mv |
Frontiers in Microbiology, v. 14. 1664-302X 10.3389/fmicb.2023.1132781 2-s2.0-85158009632 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Frontiers in Microbiology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1797790128168501248 |