Using Proteomic Approaches to Unravel the Response of Ctenocephalides felis felis to Blood Feeding and Infection With Bartonella henselae
Main Author: | |
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Publication Date: | 2022 |
Other Authors: | , , , , , , , , , , , |
Format: | Article |
Language: | eng |
Source: | Repositório Institucional da UNESP |
Download full: | http://dx.doi.org/10.3389/fcimb.2022.828082 http://hdl.handle.net/11449/230383 |
Summary: | Among the Ctenocephalides felis felis-borne pathogens, Bartonella henselae, the main aetiological agent of cat scratch disease (CSD), is of increasing comparative biomedical importance. Despite the importance of B. henselae as an emergent pathogen, prevention of the diseases caused by this agent in cats, dogs and humans mostly relies on the use of ectoparasiticides. A vaccine targeting both flea fitness and pathogen competence is an attractive choice requiring the identification of flea proteins/metabolites with a dual effect. Even though recent developments in vector and pathogen -omics have advanced the understanding of the genetic factors and molecular pathways involved at the tick-pathogen interface, leading to discovery of candidate protective antigens, only a few studies have focused on the interaction between fleas and flea-borne pathogens. Taking into account the period of time needed for B. henselae replication in flea digestive tract, the present study investigated flea-differentially abundant proteins (FDAP) in unfed fleas, fleas fed on uninfected cats, and fleas fed on B. henselae-infected cats at 24 hours and 9 days after the beginning of blood feeding. Proteomics approaches were designed and implemented to interrogate differentially expressed proteins, so as to gain a better understanding of proteomic changes associated with the initial B. henselae transmission period (24 hour timepoint) and a subsequent time point 9 days after blood ingestion and flea infection. As a result, serine proteases, ribosomal proteins, proteasome subunit α-type, juvenile hormone epoxide hydrolase 1, vitellogenin C, allantoinase, phosphoenolpyruvate carboxykinase, succinic semialdehyde dehydrogenase, glycinamide ribotide transformylase, secreted salivary acid phosphatase had high abundance in response of C. felis blood feeding and/or infection by B. henselae. In contrast, high abundance of serpin-1, arginine kinase, ribosomal proteins, peritrophin-like protein, and FS-H/FSI antigen family member 3 was strongly associated with unfed cat fleas. Findings from this study provide insights into proteomic response of cat fleas to B. henselae infected and uninfected blood meal, as well as C. felis response to invading B. henselae over an infection time course, thus helping understand the complex interactions between cat fleas and B. henselae at protein levels. |
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Using Proteomic Approaches to Unravel the Response of Ctenocephalides felis felis to Blood Feeding and Infection With Bartonella henselaebartonellosiscat fleacat scratch diseaseflea-pathogen interfaceproteomeAmong the Ctenocephalides felis felis-borne pathogens, Bartonella henselae, the main aetiological agent of cat scratch disease (CSD), is of increasing comparative biomedical importance. Despite the importance of B. henselae as an emergent pathogen, prevention of the diseases caused by this agent in cats, dogs and humans mostly relies on the use of ectoparasiticides. A vaccine targeting both flea fitness and pathogen competence is an attractive choice requiring the identification of flea proteins/metabolites with a dual effect. Even though recent developments in vector and pathogen -omics have advanced the understanding of the genetic factors and molecular pathways involved at the tick-pathogen interface, leading to discovery of candidate protective antigens, only a few studies have focused on the interaction between fleas and flea-borne pathogens. Taking into account the period of time needed for B. henselae replication in flea digestive tract, the present study investigated flea-differentially abundant proteins (FDAP) in unfed fleas, fleas fed on uninfected cats, and fleas fed on B. henselae-infected cats at 24 hours and 9 days after the beginning of blood feeding. Proteomics approaches were designed and implemented to interrogate differentially expressed proteins, so as to gain a better understanding of proteomic changes associated with the initial B. henselae transmission period (24 hour timepoint) and a subsequent time point 9 days after blood ingestion and flea infection. As a result, serine proteases, ribosomal proteins, proteasome subunit α-type, juvenile hormone epoxide hydrolase 1, vitellogenin C, allantoinase, phosphoenolpyruvate carboxykinase, succinic semialdehyde dehydrogenase, glycinamide ribotide transformylase, secreted salivary acid phosphatase had high abundance in response of C. felis blood feeding and/or infection by B. henselae. In contrast, high abundance of serpin-1, arginine kinase, ribosomal proteins, peritrophin-like protein, and FS-H/FSI antigen family member 3 was strongly associated with unfed cat fleas. Findings from this study provide insights into proteomic response of cat fleas to B. henselae infected and uninfected blood meal, as well as C. felis response to invading B. henselae over an infection time course, thus helping understand the complex interactions between cat fleas and B. henselae at protein levels.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Laboratory of Immunoparasitology Department of Pathology Reproduction and One Health Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista FCAV/UNESPIntracellular Pathogens Research Laboratory Department of Clinical Sciences The Comparative Medicine Institute College of Veterinary Medicine North Carolina State UniversityDepartment of Clinical Sciences Center for Companion Animal Studies Colorado State UniversityDepartment of Diagnostic Medicine/Pathobiology College of Veterinary Medicine Kansas State UniversityDepartment of Chemistry North Carolina State UniversityMolecular Education Technology and Research Innovation Center (METRIC) North Carolina State UniversityDepartmento de Biotecnologia Agropecuária e Ambiental Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista FCAV/UNESPLaboratory of Immunoparasitology Department of Pathology Reproduction and One Health Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista FCAV/UNESPDepartmento de Biotecnologia Agropecuária e Ambiental Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista FCAV/UNESPUniversidade Estadual Paulista (UNESP)North Carolina State UniversityColorado State UniversityKansas State UniversityAndré, Marcos Rogério [UNESP]Neupane, PradeepLappin, MichaelHerrin, BrianSmith, VickiWilliams, Taufika IslamCollins, LeonardBai, HongxiaJorge, Gabriel Lemes [UNESP]Balbuena, Tiago Santana [UNESP]Bradley, JulieMaggi, Ricardo G.Breitschwerdt, Edward B.2022-04-29T08:39:33Z2022-04-29T08:39:33Z2022-01-28info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3389/fcimb.2022.828082Frontiers in Cellular and Infection Microbiology, v. 12.2235-2988http://hdl.handle.net/11449/23038310.3389/fcimb.2022.8280822-s2.0-85124573002Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengFrontiers in Cellular and Infection Microbiologyinfo:eu-repo/semantics/openAccess2022-04-29T08:39:33Zoai:repositorio.unesp.br:11449/230383Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462022-04-29T08:39:33Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Using Proteomic Approaches to Unravel the Response of Ctenocephalides felis felis to Blood Feeding and Infection With Bartonella henselae |
title |
Using Proteomic Approaches to Unravel the Response of Ctenocephalides felis felis to Blood Feeding and Infection With Bartonella henselae |
spellingShingle |
Using Proteomic Approaches to Unravel the Response of Ctenocephalides felis felis to Blood Feeding and Infection With Bartonella henselae André, Marcos Rogério [UNESP] bartonellosis cat flea cat scratch disease flea-pathogen interface proteome |
title_short |
Using Proteomic Approaches to Unravel the Response of Ctenocephalides felis felis to Blood Feeding and Infection With Bartonella henselae |
title_full |
Using Proteomic Approaches to Unravel the Response of Ctenocephalides felis felis to Blood Feeding and Infection With Bartonella henselae |
title_fullStr |
Using Proteomic Approaches to Unravel the Response of Ctenocephalides felis felis to Blood Feeding and Infection With Bartonella henselae |
title_full_unstemmed |
Using Proteomic Approaches to Unravel the Response of Ctenocephalides felis felis to Blood Feeding and Infection With Bartonella henselae |
title_sort |
Using Proteomic Approaches to Unravel the Response of Ctenocephalides felis felis to Blood Feeding and Infection With Bartonella henselae |
author |
André, Marcos Rogério [UNESP] |
author_facet |
André, Marcos Rogério [UNESP] Neupane, Pradeep Lappin, Michael Herrin, Brian Smith, Vicki Williams, Taufika Islam Collins, Leonard Bai, Hongxia Jorge, Gabriel Lemes [UNESP] Balbuena, Tiago Santana [UNESP] Bradley, Julie Maggi, Ricardo G. Breitschwerdt, Edward B. |
author_role |
author |
author2 |
Neupane, Pradeep Lappin, Michael Herrin, Brian Smith, Vicki Williams, Taufika Islam Collins, Leonard Bai, Hongxia Jorge, Gabriel Lemes [UNESP] Balbuena, Tiago Santana [UNESP] Bradley, Julie Maggi, Ricardo G. Breitschwerdt, Edward B. |
author2_role |
author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) North Carolina State University Colorado State University Kansas State University |
dc.contributor.author.fl_str_mv |
André, Marcos Rogério [UNESP] Neupane, Pradeep Lappin, Michael Herrin, Brian Smith, Vicki Williams, Taufika Islam Collins, Leonard Bai, Hongxia Jorge, Gabriel Lemes [UNESP] Balbuena, Tiago Santana [UNESP] Bradley, Julie Maggi, Ricardo G. Breitschwerdt, Edward B. |
dc.subject.por.fl_str_mv |
bartonellosis cat flea cat scratch disease flea-pathogen interface proteome |
topic |
bartonellosis cat flea cat scratch disease flea-pathogen interface proteome |
description |
Among the Ctenocephalides felis felis-borne pathogens, Bartonella henselae, the main aetiological agent of cat scratch disease (CSD), is of increasing comparative biomedical importance. Despite the importance of B. henselae as an emergent pathogen, prevention of the diseases caused by this agent in cats, dogs and humans mostly relies on the use of ectoparasiticides. A vaccine targeting both flea fitness and pathogen competence is an attractive choice requiring the identification of flea proteins/metabolites with a dual effect. Even though recent developments in vector and pathogen -omics have advanced the understanding of the genetic factors and molecular pathways involved at the tick-pathogen interface, leading to discovery of candidate protective antigens, only a few studies have focused on the interaction between fleas and flea-borne pathogens. Taking into account the period of time needed for B. henselae replication in flea digestive tract, the present study investigated flea-differentially abundant proteins (FDAP) in unfed fleas, fleas fed on uninfected cats, and fleas fed on B. henselae-infected cats at 24 hours and 9 days after the beginning of blood feeding. Proteomics approaches were designed and implemented to interrogate differentially expressed proteins, so as to gain a better understanding of proteomic changes associated with the initial B. henselae transmission period (24 hour timepoint) and a subsequent time point 9 days after blood ingestion and flea infection. As a result, serine proteases, ribosomal proteins, proteasome subunit α-type, juvenile hormone epoxide hydrolase 1, vitellogenin C, allantoinase, phosphoenolpyruvate carboxykinase, succinic semialdehyde dehydrogenase, glycinamide ribotide transformylase, secreted salivary acid phosphatase had high abundance in response of C. felis blood feeding and/or infection by B. henselae. In contrast, high abundance of serpin-1, arginine kinase, ribosomal proteins, peritrophin-like protein, and FS-H/FSI antigen family member 3 was strongly associated with unfed cat fleas. Findings from this study provide insights into proteomic response of cat fleas to B. henselae infected and uninfected blood meal, as well as C. felis response to invading B. henselae over an infection time course, thus helping understand the complex interactions between cat fleas and B. henselae at protein levels. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-04-29T08:39:33Z 2022-04-29T08:39:33Z 2022-01-28 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3389/fcimb.2022.828082 Frontiers in Cellular and Infection Microbiology, v. 12. 2235-2988 http://hdl.handle.net/11449/230383 10.3389/fcimb.2022.828082 2-s2.0-85124573002 |
url |
http://dx.doi.org/10.3389/fcimb.2022.828082 http://hdl.handle.net/11449/230383 |
identifier_str_mv |
Frontiers in Cellular and Infection Microbiology, v. 12. 2235-2988 10.3389/fcimb.2022.828082 2-s2.0-85124573002 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Frontiers in Cellular and Infection Microbiology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1799964925719740416 |