Proteomic analysis of liver from finishing beef cattle supplemented with a rumen-protected b-vitamin blend and hydroxy trace minerals
Main Author: | |
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Publication Date: | 2021 |
Other Authors: | , , , , , , , , , |
Format: | Article |
Language: | eng |
Source: | Repositório Institucional da UNESP |
Download full: | http://dx.doi.org/10.3390/ani11071934 http://hdl.handle.net/11449/233197 |
Summary: | Vitamin B and trace minerals are crucial molecular signals involved in many biological pathways; however, their bioavailability is compromised in high-producing ruminant animals. So far, studies have mainly focused on the effects of these micronutrients on animal performance, but their use in a rumen-protected form and their impact on liver metabolism in finishing beef cattle is poorly known. We used a shotgun proteomic approach combined with biological network analyses to assess the effects of a rumen-protected B-vitamin blend, as well as those of hydroxy trace minerals, on the hepatic proteome. A total of 20 non-castrated Nellore males with 353 ± 43 kg of initial body weight were randomly assigned to one of the following treatments: CTRL—inorganic trace minerals without supplementation of a protected vitamin B blend, or SUP—supplementation of hydroxy trace minerals and a protected vitamin B blend. All animals were fed the same amount of the experimental diet for 106 days, and liver biopsies were performed at the end of the experimental period. Supplemented animals showed 37 up-regulated proteins (p < 0.10), and the enrichment analysis revealed that these proteins were involved in protein folding (p = 0.04), mitochondrial respiratory chain complex I (p = 0.01) and IV (p = 0.01), chaperonin-containing T-complex 2 (p = 0.01), glutathione metabolism (p < 0.01), and other aspects linked to oxidative-stress responses. These results indicate that rumen-protected vitamin B and hydroxy trace mineral supplementation during the finishing phase alters the abundance of proteins associated with the electron transport chain and other oxidation–reduction pathways, boosting the production of reactive oxygen species, which appear to modulate proteins linked to oxidative-damage responses to maintain cellular homeostasis. |
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Proteomic analysis of liver from finishing beef cattle supplemented with a rumen-protected b-vitamin blend and hydroxy trace mineralsBeef cattleHydroxyl trace mineralsLiver metabolismProteomicsVitamin BVitamin B and trace minerals are crucial molecular signals involved in many biological pathways; however, their bioavailability is compromised in high-producing ruminant animals. So far, studies have mainly focused on the effects of these micronutrients on animal performance, but their use in a rumen-protected form and their impact on liver metabolism in finishing beef cattle is poorly known. We used a shotgun proteomic approach combined with biological network analyses to assess the effects of a rumen-protected B-vitamin blend, as well as those of hydroxy trace minerals, on the hepatic proteome. A total of 20 non-castrated Nellore males with 353 ± 43 kg of initial body weight were randomly assigned to one of the following treatments: CTRL—inorganic trace minerals without supplementation of a protected vitamin B blend, or SUP—supplementation of hydroxy trace minerals and a protected vitamin B blend. All animals were fed the same amount of the experimental diet for 106 days, and liver biopsies were performed at the end of the experimental period. Supplemented animals showed 37 up-regulated proteins (p < 0.10), and the enrichment analysis revealed that these proteins were involved in protein folding (p = 0.04), mitochondrial respiratory chain complex I (p = 0.01) and IV (p = 0.01), chaperonin-containing T-complex 2 (p = 0.01), glutathione metabolism (p < 0.01), and other aspects linked to oxidative-stress responses. These results indicate that rumen-protected vitamin B and hydroxy trace mineral supplementation during the finishing phase alters the abundance of proteins associated with the electron transport chain and other oxidation–reduction pathways, boosting the production of reactive oxygen species, which appear to modulate proteins linked to oxidative-damage responses to maintain cellular homeostasis.Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Department of Animal Science Universidade Federal de ViçosaMuscle Biology and Nutrigenomics Laboratory Universidade Federal de ViçosaDepartment of Animal Science São Paulo State UniversityTrouw Nutrition Research and DevelopmentDepartment of Animal Science Iowa State UniversityDepartment of Biochemistry and Molecular Biology Universidade Federal de ViçosaDepartment of Animal Science São Paulo State UniversityCAPES: 001CNPq: 31883/2018-4Universidade Federal de Viçosa (UFV)Universidade Estadual Paulista (UNESP)Research and DevelopmentIowa State UniversityLopes, Mariana MescoutoBrito, Thaís Ribeiro [UNESP]Lage, Josiane FonsecaCosta, Thaís CorreiaFontes, Marta Maria Dos SantosSerão, Nick Vergara LopesMendes, Tiago Antônio de OliveiraReis, Ricardo Andrade [UNESP]Veroneze, RenataE Silva, Fabyano FonsecaDuarte, Marcio de Souza2022-05-01T05:29:35Z2022-05-01T05:29:35Z2021-07-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3390/ani11071934Animals, v. 11, n. 7, 2021.2076-2615http://hdl.handle.net/11449/23319710.3390/ani110719342-s2.0-85108706060Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengAnimalsinfo:eu-repo/semantics/openAccess2024-06-07T18:40:39Zoai:repositorio.unesp.br:11449/233197Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:52:59.429770Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Proteomic analysis of liver from finishing beef cattle supplemented with a rumen-protected b-vitamin blend and hydroxy trace minerals |
title |
Proteomic analysis of liver from finishing beef cattle supplemented with a rumen-protected b-vitamin blend and hydroxy trace minerals |
spellingShingle |
Proteomic analysis of liver from finishing beef cattle supplemented with a rumen-protected b-vitamin blend and hydroxy trace minerals Lopes, Mariana Mescouto Beef cattle Hydroxyl trace minerals Liver metabolism Proteomics Vitamin B |
title_short |
Proteomic analysis of liver from finishing beef cattle supplemented with a rumen-protected b-vitamin blend and hydroxy trace minerals |
title_full |
Proteomic analysis of liver from finishing beef cattle supplemented with a rumen-protected b-vitamin blend and hydroxy trace minerals |
title_fullStr |
Proteomic analysis of liver from finishing beef cattle supplemented with a rumen-protected b-vitamin blend and hydroxy trace minerals |
title_full_unstemmed |
Proteomic analysis of liver from finishing beef cattle supplemented with a rumen-protected b-vitamin blend and hydroxy trace minerals |
title_sort |
Proteomic analysis of liver from finishing beef cattle supplemented with a rumen-protected b-vitamin blend and hydroxy trace minerals |
author |
Lopes, Mariana Mescouto |
author_facet |
Lopes, Mariana Mescouto Brito, Thaís Ribeiro [UNESP] Lage, Josiane Fonseca Costa, Thaís Correia Fontes, Marta Maria Dos Santos Serão, Nick Vergara Lopes Mendes, Tiago Antônio de Oliveira Reis, Ricardo Andrade [UNESP] Veroneze, Renata E Silva, Fabyano Fonseca Duarte, Marcio de Souza |
author_role |
author |
author2 |
Brito, Thaís Ribeiro [UNESP] Lage, Josiane Fonseca Costa, Thaís Correia Fontes, Marta Maria Dos Santos Serão, Nick Vergara Lopes Mendes, Tiago Antônio de Oliveira Reis, Ricardo Andrade [UNESP] Veroneze, Renata E Silva, Fabyano Fonseca Duarte, Marcio de Souza |
author2_role |
author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de Viçosa (UFV) Universidade Estadual Paulista (UNESP) Research and Development Iowa State University |
dc.contributor.author.fl_str_mv |
Lopes, Mariana Mescouto Brito, Thaís Ribeiro [UNESP] Lage, Josiane Fonseca Costa, Thaís Correia Fontes, Marta Maria Dos Santos Serão, Nick Vergara Lopes Mendes, Tiago Antônio de Oliveira Reis, Ricardo Andrade [UNESP] Veroneze, Renata E Silva, Fabyano Fonseca Duarte, Marcio de Souza |
dc.subject.por.fl_str_mv |
Beef cattle Hydroxyl trace minerals Liver metabolism Proteomics Vitamin B |
topic |
Beef cattle Hydroxyl trace minerals Liver metabolism Proteomics Vitamin B |
description |
Vitamin B and trace minerals are crucial molecular signals involved in many biological pathways; however, their bioavailability is compromised in high-producing ruminant animals. So far, studies have mainly focused on the effects of these micronutrients on animal performance, but their use in a rumen-protected form and their impact on liver metabolism in finishing beef cattle is poorly known. We used a shotgun proteomic approach combined with biological network analyses to assess the effects of a rumen-protected B-vitamin blend, as well as those of hydroxy trace minerals, on the hepatic proteome. A total of 20 non-castrated Nellore males with 353 ± 43 kg of initial body weight were randomly assigned to one of the following treatments: CTRL—inorganic trace minerals without supplementation of a protected vitamin B blend, or SUP—supplementation of hydroxy trace minerals and a protected vitamin B blend. All animals were fed the same amount of the experimental diet for 106 days, and liver biopsies were performed at the end of the experimental period. Supplemented animals showed 37 up-regulated proteins (p < 0.10), and the enrichment analysis revealed that these proteins were involved in protein folding (p = 0.04), mitochondrial respiratory chain complex I (p = 0.01) and IV (p = 0.01), chaperonin-containing T-complex 2 (p = 0.01), glutathione metabolism (p < 0.01), and other aspects linked to oxidative-stress responses. These results indicate that rumen-protected vitamin B and hydroxy trace mineral supplementation during the finishing phase alters the abundance of proteins associated with the electron transport chain and other oxidation–reduction pathways, boosting the production of reactive oxygen species, which appear to modulate proteins linked to oxidative-damage responses to maintain cellular homeostasis. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-07-01 2022-05-01T05:29:35Z 2022-05-01T05:29:35Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3390/ani11071934 Animals, v. 11, n. 7, 2021. 2076-2615 http://hdl.handle.net/11449/233197 10.3390/ani11071934 2-s2.0-85108706060 |
url |
http://dx.doi.org/10.3390/ani11071934 http://hdl.handle.net/11449/233197 |
identifier_str_mv |
Animals, v. 11, n. 7, 2021. 2076-2615 10.3390/ani11071934 2-s2.0-85108706060 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Animals |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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_version_ |
1808128578223603712 |