Proteomic analysis of liver from finishing beef cattle supplemented with a rumen-protected b-vitamin blend and hydroxy trace minerals

Bibliographic Details
Main Author: Lopes, Mariana Mescouto
Publication Date: 2021
Other Authors: Brito, Thaís Ribeiro [UNESP], Lage, Josiane Fonseca, Costa, Thaís Correia, Fontes, Marta Maria Dos Santos, Serão, Nick Vergara Lopes, Mendes, Tiago Antônio de Oliveira, Reis, Ricardo Andrade [UNESP], Veroneze, Renata, E Silva, Fabyano Fonseca, Duarte, Marcio de Souza
Format: Article
Language: eng
Source: Repositório Institucional da UNESP
Download full: http://dx.doi.org/10.3390/ani11071934
http://hdl.handle.net/11449/233197
Summary: Vitamin B and trace minerals are crucial molecular signals involved in many biological pathways; however, their bioavailability is compromised in high-producing ruminant animals. So far, studies have mainly focused on the effects of these micronutrients on animal performance, but their use in a rumen-protected form and their impact on liver metabolism in finishing beef cattle is poorly known. We used a shotgun proteomic approach combined with biological network analyses to assess the effects of a rumen-protected B-vitamin blend, as well as those of hydroxy trace minerals, on the hepatic proteome. A total of 20 non-castrated Nellore males with 353 ± 43 kg of initial body weight were randomly assigned to one of the following treatments: CTRL—inorganic trace minerals without supplementation of a protected vitamin B blend, or SUP—supplementation of hydroxy trace minerals and a protected vitamin B blend. All animals were fed the same amount of the experimental diet for 106 days, and liver biopsies were performed at the end of the experimental period. Supplemented animals showed 37 up-regulated proteins (p < 0.10), and the enrichment analysis revealed that these proteins were involved in protein folding (p = 0.04), mitochondrial respiratory chain complex I (p = 0.01) and IV (p = 0.01), chaperonin-containing T-complex 2 (p = 0.01), glutathione metabolism (p < 0.01), and other aspects linked to oxidative-stress responses. These results indicate that rumen-protected vitamin B and hydroxy trace mineral supplementation during the finishing phase alters the abundance of proteins associated with the electron transport chain and other oxidation–reduction pathways, boosting the production of reactive oxygen species, which appear to modulate proteins linked to oxidative-damage responses to maintain cellular homeostasis.
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spelling Proteomic analysis of liver from finishing beef cattle supplemented with a rumen-protected b-vitamin blend and hydroxy trace mineralsBeef cattleHydroxyl trace mineralsLiver metabolismProteomicsVitamin BVitamin B and trace minerals are crucial molecular signals involved in many biological pathways; however, their bioavailability is compromised in high-producing ruminant animals. So far, studies have mainly focused on the effects of these micronutrients on animal performance, but their use in a rumen-protected form and their impact on liver metabolism in finishing beef cattle is poorly known. We used a shotgun proteomic approach combined with biological network analyses to assess the effects of a rumen-protected B-vitamin blend, as well as those of hydroxy trace minerals, on the hepatic proteome. A total of 20 non-castrated Nellore males with 353 ± 43 kg of initial body weight were randomly assigned to one of the following treatments: CTRL—inorganic trace minerals without supplementation of a protected vitamin B blend, or SUP—supplementation of hydroxy trace minerals and a protected vitamin B blend. All animals were fed the same amount of the experimental diet for 106 days, and liver biopsies were performed at the end of the experimental period. Supplemented animals showed 37 up-regulated proteins (p < 0.10), and the enrichment analysis revealed that these proteins were involved in protein folding (p = 0.04), mitochondrial respiratory chain complex I (p = 0.01) and IV (p = 0.01), chaperonin-containing T-complex 2 (p = 0.01), glutathione metabolism (p < 0.01), and other aspects linked to oxidative-stress responses. These results indicate that rumen-protected vitamin B and hydroxy trace mineral supplementation during the finishing phase alters the abundance of proteins associated with the electron transport chain and other oxidation–reduction pathways, boosting the production of reactive oxygen species, which appear to modulate proteins linked to oxidative-damage responses to maintain cellular homeostasis.Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Department of Animal Science Universidade Federal de ViçosaMuscle Biology and Nutrigenomics Laboratory Universidade Federal de ViçosaDepartment of Animal Science São Paulo State UniversityTrouw Nutrition Research and DevelopmentDepartment of Animal Science Iowa State UniversityDepartment of Biochemistry and Molecular Biology Universidade Federal de ViçosaDepartment of Animal Science São Paulo State UniversityCAPES: 001CNPq: 31883/2018-4Universidade Federal de Viçosa (UFV)Universidade Estadual Paulista (UNESP)Research and DevelopmentIowa State UniversityLopes, Mariana MescoutoBrito, Thaís Ribeiro [UNESP]Lage, Josiane FonsecaCosta, Thaís CorreiaFontes, Marta Maria Dos SantosSerão, Nick Vergara LopesMendes, Tiago Antônio de OliveiraReis, Ricardo Andrade [UNESP]Veroneze, RenataE Silva, Fabyano FonsecaDuarte, Marcio de Souza2022-05-01T05:29:35Z2022-05-01T05:29:35Z2021-07-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3390/ani11071934Animals, v. 11, n. 7, 2021.2076-2615http://hdl.handle.net/11449/23319710.3390/ani110719342-s2.0-85108706060Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengAnimalsinfo:eu-repo/semantics/openAccess2024-06-07T18:40:39Zoai:repositorio.unesp.br:11449/233197Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:52:59.429770Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Proteomic analysis of liver from finishing beef cattle supplemented with a rumen-protected b-vitamin blend and hydroxy trace minerals
title Proteomic analysis of liver from finishing beef cattle supplemented with a rumen-protected b-vitamin blend and hydroxy trace minerals
spellingShingle Proteomic analysis of liver from finishing beef cattle supplemented with a rumen-protected b-vitamin blend and hydroxy trace minerals
Lopes, Mariana Mescouto
Beef cattle
Hydroxyl trace minerals
Liver metabolism
Proteomics
Vitamin B
title_short Proteomic analysis of liver from finishing beef cattle supplemented with a rumen-protected b-vitamin blend and hydroxy trace minerals
title_full Proteomic analysis of liver from finishing beef cattle supplemented with a rumen-protected b-vitamin blend and hydroxy trace minerals
title_fullStr Proteomic analysis of liver from finishing beef cattle supplemented with a rumen-protected b-vitamin blend and hydroxy trace minerals
title_full_unstemmed Proteomic analysis of liver from finishing beef cattle supplemented with a rumen-protected b-vitamin blend and hydroxy trace minerals
title_sort Proteomic analysis of liver from finishing beef cattle supplemented with a rumen-protected b-vitamin blend and hydroxy trace minerals
author Lopes, Mariana Mescouto
author_facet Lopes, Mariana Mescouto
Brito, Thaís Ribeiro [UNESP]
Lage, Josiane Fonseca
Costa, Thaís Correia
Fontes, Marta Maria Dos Santos
Serão, Nick Vergara Lopes
Mendes, Tiago Antônio de Oliveira
Reis, Ricardo Andrade [UNESP]
Veroneze, Renata
E Silva, Fabyano Fonseca
Duarte, Marcio de Souza
author_role author
author2 Brito, Thaís Ribeiro [UNESP]
Lage, Josiane Fonseca
Costa, Thaís Correia
Fontes, Marta Maria Dos Santos
Serão, Nick Vergara Lopes
Mendes, Tiago Antônio de Oliveira
Reis, Ricardo Andrade [UNESP]
Veroneze, Renata
E Silva, Fabyano Fonseca
Duarte, Marcio de Souza
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Federal de Viçosa (UFV)
Universidade Estadual Paulista (UNESP)
Research and Development
Iowa State University
dc.contributor.author.fl_str_mv Lopes, Mariana Mescouto
Brito, Thaís Ribeiro [UNESP]
Lage, Josiane Fonseca
Costa, Thaís Correia
Fontes, Marta Maria Dos Santos
Serão, Nick Vergara Lopes
Mendes, Tiago Antônio de Oliveira
Reis, Ricardo Andrade [UNESP]
Veroneze, Renata
E Silva, Fabyano Fonseca
Duarte, Marcio de Souza
dc.subject.por.fl_str_mv Beef cattle
Hydroxyl trace minerals
Liver metabolism
Proteomics
Vitamin B
topic Beef cattle
Hydroxyl trace minerals
Liver metabolism
Proteomics
Vitamin B
description Vitamin B and trace minerals are crucial molecular signals involved in many biological pathways; however, their bioavailability is compromised in high-producing ruminant animals. So far, studies have mainly focused on the effects of these micronutrients on animal performance, but their use in a rumen-protected form and their impact on liver metabolism in finishing beef cattle is poorly known. We used a shotgun proteomic approach combined with biological network analyses to assess the effects of a rumen-protected B-vitamin blend, as well as those of hydroxy trace minerals, on the hepatic proteome. A total of 20 non-castrated Nellore males with 353 ± 43 kg of initial body weight were randomly assigned to one of the following treatments: CTRL—inorganic trace minerals without supplementation of a protected vitamin B blend, or SUP—supplementation of hydroxy trace minerals and a protected vitamin B blend. All animals were fed the same amount of the experimental diet for 106 days, and liver biopsies were performed at the end of the experimental period. Supplemented animals showed 37 up-regulated proteins (p < 0.10), and the enrichment analysis revealed that these proteins were involved in protein folding (p = 0.04), mitochondrial respiratory chain complex I (p = 0.01) and IV (p = 0.01), chaperonin-containing T-complex 2 (p = 0.01), glutathione metabolism (p < 0.01), and other aspects linked to oxidative-stress responses. These results indicate that rumen-protected vitamin B and hydroxy trace mineral supplementation during the finishing phase alters the abundance of proteins associated with the electron transport chain and other oxidation–reduction pathways, boosting the production of reactive oxygen species, which appear to modulate proteins linked to oxidative-damage responses to maintain cellular homeostasis.
publishDate 2021
dc.date.none.fl_str_mv 2021-07-01
2022-05-01T05:29:35Z
2022-05-01T05:29:35Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.3390/ani11071934
Animals, v. 11, n. 7, 2021.
2076-2615
http://hdl.handle.net/11449/233197
10.3390/ani11071934
2-s2.0-85108706060
url http://dx.doi.org/10.3390/ani11071934
http://hdl.handle.net/11449/233197
identifier_str_mv Animals, v. 11, n. 7, 2021.
2076-2615
10.3390/ani11071934
2-s2.0-85108706060
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Animals
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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