Evaluation of the Cytotoxicity and Biocompatibility of New Resin Epoxy–based Endodontic Sealer Containing Calcium Hydroxide
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.joen.2017.07.016 http://hdl.handle.net/11449/179257 |
Resumo: | Introduction Many endodontic sealers are available, but the search for the ideal sealer continues. This study evaluated the cytotoxicity and biocompatibility of Sealer Plus, a new resin epoxy–based endodontic sealer containing calcium hydroxide. AH Plus, Endofill, and SimpliSeal endodontics sealers were used for comparison. Methods L929 fibroblasts were cultured, and an MTT assay was used to determine the cytotoxicity of the sealer extracts at 6, 24, 48, and 72 hours. Tubes containing materials or empty tubes for control were inserted into the subcutaneous tissues of 20 rats. After 7 and 30 days, the rats were killed, and the tubes were removed with the surrounding tissues for histologic analysis. The data were submitted to statistical tests (P <.05). Results Undiluted Sealer Plus exhibited less cytotoxicity compared with other undiluted extracts at 6 hours (P <.05), and cell viability was higher for all Sealer Plus extracts after 24 hours (P <.05). At 48 hours, the undiluted and ½ Sealer Plus dilution were the extracts with less cytotoxicity (P <.05). At 72 hours, cell viability was higher for the undiluted and ½ Sealer Plus dilution compared with the other sealers (P <.05). At 7 days, Endofill and SimpliSeal had higher inflammation compared with the control and Sealer Plus (P <.05); AH Plus had moderate inflammation (P >.05). At 30 days, control, Sealer Plus, and AH Plus had less inflammation (P <.05). The fibrous capsule was thick at 7 days and thin at 30 days, except for SimpliSeal. Conclusions In general, Sealer Plus promoted greater cell viability and was more biocompatible compared with the other sealers. |
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Repositório Institucional da UNESP |
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Evaluation of the Cytotoxicity and Biocompatibility of New Resin Epoxy–based Endodontic Sealer Containing Calcium HydroxideBiocompatibilitycalcium hydroxidecytotoxicityendodontic sealersresin epoxyIntroduction Many endodontic sealers are available, but the search for the ideal sealer continues. This study evaluated the cytotoxicity and biocompatibility of Sealer Plus, a new resin epoxy–based endodontic sealer containing calcium hydroxide. AH Plus, Endofill, and SimpliSeal endodontics sealers were used for comparison. Methods L929 fibroblasts were cultured, and an MTT assay was used to determine the cytotoxicity of the sealer extracts at 6, 24, 48, and 72 hours. Tubes containing materials or empty tubes for control were inserted into the subcutaneous tissues of 20 rats. After 7 and 30 days, the rats were killed, and the tubes were removed with the surrounding tissues for histologic analysis. The data were submitted to statistical tests (P <.05). Results Undiluted Sealer Plus exhibited less cytotoxicity compared with other undiluted extracts at 6 hours (P <.05), and cell viability was higher for all Sealer Plus extracts after 24 hours (P <.05). At 48 hours, the undiluted and ½ Sealer Plus dilution were the extracts with less cytotoxicity (P <.05). At 72 hours, cell viability was higher for the undiluted and ½ Sealer Plus dilution compared with the other sealers (P <.05). At 7 days, Endofill and SimpliSeal had higher inflammation compared with the control and Sealer Plus (P <.05); AH Plus had moderate inflammation (P >.05). At 30 days, control, Sealer Plus, and AH Plus had less inflammation (P <.05). The fibrous capsule was thick at 7 days and thin at 30 days, except for SimpliSeal. Conclusions In general, Sealer Plus promoted greater cell viability and was more biocompatible compared with the other sealers.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Department of Endodontics São Paulo State University (Unesp) School of DentistryDepartment of Basic Science São Paulo State University (Unesp) School of DentistryDepartment of Endodontics São Paulo State University (Unesp) School of DentistryDepartment of Basic Science São Paulo State University (Unesp) School of DentistryCNPq: 305969/2015-3Universidade Estadual Paulista (Unesp)Cintra, Luciano Tavares Angelo [UNESP]Benetti, Francine [UNESP]de Azevedo Queiroz, Índia Olinta [UNESP]Ferreira, Luciana Louzada [UNESP]Massunari, Loiane [UNESP]Bueno, Carlos Roberto Emerenciano [UNESP]de Oliveira, Sandra Helena Penha [UNESP]Gomes-Filho, João Eduardo [UNESP]2018-12-11T17:34:24Z2018-12-11T17:34:24Z2017-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2088-2092application/pdfhttp://dx.doi.org/10.1016/j.joen.2017.07.016Journal of Endodontics, v. 43, n. 12, p. 2088-2092, 2017.0099-2399http://hdl.handle.net/11449/17925710.1016/j.joen.2017.07.0162-s2.0-850306970102-s2.0-85030697010.pdf9235743081667362Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Endodontics1,585info:eu-repo/semantics/openAccess2023-10-13T06:07:24Zoai:repositorio.unesp.br:11449/179257Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-10-13T06:07:24Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Evaluation of the Cytotoxicity and Biocompatibility of New Resin Epoxy–based Endodontic Sealer Containing Calcium Hydroxide |
title |
Evaluation of the Cytotoxicity and Biocompatibility of New Resin Epoxy–based Endodontic Sealer Containing Calcium Hydroxide |
spellingShingle |
Evaluation of the Cytotoxicity and Biocompatibility of New Resin Epoxy–based Endodontic Sealer Containing Calcium Hydroxide Cintra, Luciano Tavares Angelo [UNESP] Biocompatibility calcium hydroxide cytotoxicity endodontic sealers resin epoxy |
title_short |
Evaluation of the Cytotoxicity and Biocompatibility of New Resin Epoxy–based Endodontic Sealer Containing Calcium Hydroxide |
title_full |
Evaluation of the Cytotoxicity and Biocompatibility of New Resin Epoxy–based Endodontic Sealer Containing Calcium Hydroxide |
title_fullStr |
Evaluation of the Cytotoxicity and Biocompatibility of New Resin Epoxy–based Endodontic Sealer Containing Calcium Hydroxide |
title_full_unstemmed |
Evaluation of the Cytotoxicity and Biocompatibility of New Resin Epoxy–based Endodontic Sealer Containing Calcium Hydroxide |
title_sort |
Evaluation of the Cytotoxicity and Biocompatibility of New Resin Epoxy–based Endodontic Sealer Containing Calcium Hydroxide |
author |
Cintra, Luciano Tavares Angelo [UNESP] |
author_facet |
Cintra, Luciano Tavares Angelo [UNESP] Benetti, Francine [UNESP] de Azevedo Queiroz, Índia Olinta [UNESP] Ferreira, Luciana Louzada [UNESP] Massunari, Loiane [UNESP] Bueno, Carlos Roberto Emerenciano [UNESP] de Oliveira, Sandra Helena Penha [UNESP] Gomes-Filho, João Eduardo [UNESP] |
author_role |
author |
author2 |
Benetti, Francine [UNESP] de Azevedo Queiroz, Índia Olinta [UNESP] Ferreira, Luciana Louzada [UNESP] Massunari, Loiane [UNESP] Bueno, Carlos Roberto Emerenciano [UNESP] de Oliveira, Sandra Helena Penha [UNESP] Gomes-Filho, João Eduardo [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Cintra, Luciano Tavares Angelo [UNESP] Benetti, Francine [UNESP] de Azevedo Queiroz, Índia Olinta [UNESP] Ferreira, Luciana Louzada [UNESP] Massunari, Loiane [UNESP] Bueno, Carlos Roberto Emerenciano [UNESP] de Oliveira, Sandra Helena Penha [UNESP] Gomes-Filho, João Eduardo [UNESP] |
dc.subject.por.fl_str_mv |
Biocompatibility calcium hydroxide cytotoxicity endodontic sealers resin epoxy |
topic |
Biocompatibility calcium hydroxide cytotoxicity endodontic sealers resin epoxy |
description |
Introduction Many endodontic sealers are available, but the search for the ideal sealer continues. This study evaluated the cytotoxicity and biocompatibility of Sealer Plus, a new resin epoxy–based endodontic sealer containing calcium hydroxide. AH Plus, Endofill, and SimpliSeal endodontics sealers were used for comparison. Methods L929 fibroblasts were cultured, and an MTT assay was used to determine the cytotoxicity of the sealer extracts at 6, 24, 48, and 72 hours. Tubes containing materials or empty tubes for control were inserted into the subcutaneous tissues of 20 rats. After 7 and 30 days, the rats were killed, and the tubes were removed with the surrounding tissues for histologic analysis. The data were submitted to statistical tests (P <.05). Results Undiluted Sealer Plus exhibited less cytotoxicity compared with other undiluted extracts at 6 hours (P <.05), and cell viability was higher for all Sealer Plus extracts after 24 hours (P <.05). At 48 hours, the undiluted and ½ Sealer Plus dilution were the extracts with less cytotoxicity (P <.05). At 72 hours, cell viability was higher for the undiluted and ½ Sealer Plus dilution compared with the other sealers (P <.05). At 7 days, Endofill and SimpliSeal had higher inflammation compared with the control and Sealer Plus (P <.05); AH Plus had moderate inflammation (P >.05). At 30 days, control, Sealer Plus, and AH Plus had less inflammation (P <.05). The fibrous capsule was thick at 7 days and thin at 30 days, except for SimpliSeal. Conclusions In general, Sealer Plus promoted greater cell viability and was more biocompatible compared with the other sealers. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-12-01 2018-12-11T17:34:24Z 2018-12-11T17:34:24Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.joen.2017.07.016 Journal of Endodontics, v. 43, n. 12, p. 2088-2092, 2017. 0099-2399 http://hdl.handle.net/11449/179257 10.1016/j.joen.2017.07.016 2-s2.0-85030697010 2-s2.0-85030697010.pdf 9235743081667362 |
url |
http://dx.doi.org/10.1016/j.joen.2017.07.016 http://hdl.handle.net/11449/179257 |
identifier_str_mv |
Journal of Endodontics, v. 43, n. 12, p. 2088-2092, 2017. 0099-2399 10.1016/j.joen.2017.07.016 2-s2.0-85030697010 2-s2.0-85030697010.pdf 9235743081667362 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Endodontics 1,585 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
2088-2092 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1797789382486261760 |