Ellagic Acid-Cyclodextrin Complexes for the Treatment of Oral Candidiasis
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Publication Date: | 2021 |
Other Authors: | , , , , |
Format: | Article |
Language: | eng |
Source: | Repositório Institucional da UNESP |
Download full: | http://dx.doi.org/10.3390/molecules26020505 http://hdl.handle.net/11449/207222 |
Summary: | The increase in the prevalence of fungal infections worldwide and the rise in the occurrence of antifungal resistance suggest that new research to discover antifungal molecules is needed. The aim of this study was to evaluate the potential use of ellagic acid-cyclodextrin complexes (EA/HP-β-CD) for the treatment of oral candidiasis. First, the effect of EA/HP-β-CD on C. albicans planktonic cells and biofilms was evaluated. Then, the cytotoxicity of the effective concentration was studied to ensure safety of in vivo testing. Finally, the in vivo effectiveness was determined by using a murine model of induced oral candidiasis. Data was statistically analyzed. The minimal inhibitory concentration of EA/HP-β-CD was 25 µg/mL and a concentration of 10 times MIC (250 µg/mL) showed an inhibitory effect on C. albicans 48 h-biofilms. The complex at concentration 250 µg/mL was classified as slightly cytotoxic. In vivo experiments showed a reduction in fungal epithelial invasion after treatment with EA/HP-β-CD for 24 h and 96 h when compared to the negative control. In conclusion, the results demonstrated that EA/HP-β-CD has antifungal and anti-inflammatory effects, reducing the invasive capacity of C. albicans, which suggests that EA/HP-β-CD may be a promising alternative for the treatment of oral candidiasis. |
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Ellagic Acid-Cyclodextrin Complexes for the Treatment of Oral CandidiasisCandida albicanscyclodextrinellagic acidoral candidiasisThe increase in the prevalence of fungal infections worldwide and the rise in the occurrence of antifungal resistance suggest that new research to discover antifungal molecules is needed. The aim of this study was to evaluate the potential use of ellagic acid-cyclodextrin complexes (EA/HP-β-CD) for the treatment of oral candidiasis. First, the effect of EA/HP-β-CD on C. albicans planktonic cells and biofilms was evaluated. Then, the cytotoxicity of the effective concentration was studied to ensure safety of in vivo testing. Finally, the in vivo effectiveness was determined by using a murine model of induced oral candidiasis. Data was statistically analyzed. The minimal inhibitory concentration of EA/HP-β-CD was 25 µg/mL and a concentration of 10 times MIC (250 µg/mL) showed an inhibitory effect on C. albicans 48 h-biofilms. The complex at concentration 250 µg/mL was classified as slightly cytotoxic. In vivo experiments showed a reduction in fungal epithelial invasion after treatment with EA/HP-β-CD for 24 h and 96 h when compared to the negative control. In conclusion, the results demonstrated that EA/HP-β-CD has antifungal and anti-inflammatory effects, reducing the invasive capacity of C. albicans, which suggests that EA/HP-β-CD may be a promising alternative for the treatment of oral candidiasis.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Oral Biopathology Graduate Program São José dos Campos Institute of Science & Technology São Paulo State University UNESPSchool of Dentistry Santo Amaro UniversityDepartment of Environment Engineering São José dos Campos Institute of Science & Technology São Paulo State University UNESPOral Biopathology Graduate Program São José dos Campos Institute of Science & Technology São Paulo State University UNESPDepartment of Environment Engineering São José dos Campos Institute of Science & Technology São Paulo State University UNESPCNPq: 308127/2018-8CAPES: Finance Code 001FAPESP: Grants 16/212017-0 and 2016/07196-6Universidade Estadual Paulista (Unesp)Santo Amaro UniversitySampaio, Aline da Graça [UNESP]Gontijo, Aline Vidal Lacerda [UNESP]Lima, Gabriela de Morais Gouvêa [UNESP]de Oliveira, Maria Alcionéia Carvalho [UNESP]Lepesqueur, Laura Soares Souto [UNESP]Koga-Ito, Cristiane Yumi [UNESP]2021-06-25T10:50:57Z2021-06-25T10:50:57Z2021-01-19info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3390/molecules26020505Molecules (Basel, Switzerland), v. 26, n. 2, 2021.1420-3049http://hdl.handle.net/11449/20722210.3390/molecules260205052-s2.0-8510032779565435631614034210000-0002-2416-2173Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMolecules (Basel, Switzerland)info:eu-repo/semantics/openAccess2022-03-16T01:17:24Zoai:repositorio.unesp.br:11449/207222Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462022-03-16T01:17:24Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Ellagic Acid-Cyclodextrin Complexes for the Treatment of Oral Candidiasis |
title |
Ellagic Acid-Cyclodextrin Complexes for the Treatment of Oral Candidiasis |
spellingShingle |
Ellagic Acid-Cyclodextrin Complexes for the Treatment of Oral Candidiasis Sampaio, Aline da Graça [UNESP] Candida albicans cyclodextrin ellagic acid oral candidiasis |
title_short |
Ellagic Acid-Cyclodextrin Complexes for the Treatment of Oral Candidiasis |
title_full |
Ellagic Acid-Cyclodextrin Complexes for the Treatment of Oral Candidiasis |
title_fullStr |
Ellagic Acid-Cyclodextrin Complexes for the Treatment of Oral Candidiasis |
title_full_unstemmed |
Ellagic Acid-Cyclodextrin Complexes for the Treatment of Oral Candidiasis |
title_sort |
Ellagic Acid-Cyclodextrin Complexes for the Treatment of Oral Candidiasis |
author |
Sampaio, Aline da Graça [UNESP] |
author_facet |
Sampaio, Aline da Graça [UNESP] Gontijo, Aline Vidal Lacerda [UNESP] Lima, Gabriela de Morais Gouvêa [UNESP] de Oliveira, Maria Alcionéia Carvalho [UNESP] Lepesqueur, Laura Soares Souto [UNESP] Koga-Ito, Cristiane Yumi [UNESP] |
author_role |
author |
author2 |
Gontijo, Aline Vidal Lacerda [UNESP] Lima, Gabriela de Morais Gouvêa [UNESP] de Oliveira, Maria Alcionéia Carvalho [UNESP] Lepesqueur, Laura Soares Souto [UNESP] Koga-Ito, Cristiane Yumi [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Santo Amaro University |
dc.contributor.author.fl_str_mv |
Sampaio, Aline da Graça [UNESP] Gontijo, Aline Vidal Lacerda [UNESP] Lima, Gabriela de Morais Gouvêa [UNESP] de Oliveira, Maria Alcionéia Carvalho [UNESP] Lepesqueur, Laura Soares Souto [UNESP] Koga-Ito, Cristiane Yumi [UNESP] |
dc.subject.por.fl_str_mv |
Candida albicans cyclodextrin ellagic acid oral candidiasis |
topic |
Candida albicans cyclodextrin ellagic acid oral candidiasis |
description |
The increase in the prevalence of fungal infections worldwide and the rise in the occurrence of antifungal resistance suggest that new research to discover antifungal molecules is needed. The aim of this study was to evaluate the potential use of ellagic acid-cyclodextrin complexes (EA/HP-β-CD) for the treatment of oral candidiasis. First, the effect of EA/HP-β-CD on C. albicans planktonic cells and biofilms was evaluated. Then, the cytotoxicity of the effective concentration was studied to ensure safety of in vivo testing. Finally, the in vivo effectiveness was determined by using a murine model of induced oral candidiasis. Data was statistically analyzed. The minimal inhibitory concentration of EA/HP-β-CD was 25 µg/mL and a concentration of 10 times MIC (250 µg/mL) showed an inhibitory effect on C. albicans 48 h-biofilms. The complex at concentration 250 µg/mL was classified as slightly cytotoxic. In vivo experiments showed a reduction in fungal epithelial invasion after treatment with EA/HP-β-CD for 24 h and 96 h when compared to the negative control. In conclusion, the results demonstrated that EA/HP-β-CD has antifungal and anti-inflammatory effects, reducing the invasive capacity of C. albicans, which suggests that EA/HP-β-CD may be a promising alternative for the treatment of oral candidiasis. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-25T10:50:57Z 2021-06-25T10:50:57Z 2021-01-19 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3390/molecules26020505 Molecules (Basel, Switzerland), v. 26, n. 2, 2021. 1420-3049 http://hdl.handle.net/11449/207222 10.3390/molecules26020505 2-s2.0-85100327795 6543563161403421 0000-0002-2416-2173 |
url |
http://dx.doi.org/10.3390/molecules26020505 http://hdl.handle.net/11449/207222 |
identifier_str_mv |
Molecules (Basel, Switzerland), v. 26, n. 2, 2021. 1420-3049 10.3390/molecules26020505 2-s2.0-85100327795 6543563161403421 0000-0002-2416-2173 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Molecules (Basel, Switzerland) |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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