Maternal Protein Restriction Modulates Angiogenesis and AQP9 Expression Leading to a Delay in Postnatal Epididymal Development in Rat
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3390/cells8091094 http://hdl.handle.net/11449/201288 |
Resumo: | The maternal nutritional status is essential to the health and well-being of the fetus. Maternal protein restriction during the perinatal stage causes sperm alterations in the offspring that are associated with epididymal dysfunctions. Vascular endothelial growth factor (VEGF) and its receptor, VEGFr-2, as well as aquaporins (AQPs) are important regulators of angiogenesis and the epididymal microenvironment and are associated with male fertility. We investigated the effects of maternal protein restriction on epididymal angiogenesis and AQP expression in the early stages of postnatal epididymal development. Pregnant rats were divided into two experimental groups that received either a normoprotein (17% protein) or low-protein diet (6% protein) during gestation and lactation. At postnatal day (PND)7 and PND14, male offspring were euthanized, the epididymides were subjected to morphometric and microvascular density analyses and to VEGF-A, VEGF-r2, AQP1 and AQP9 expression analyses. The maternal low-protein diet decreased AQP9 and VEGFr-2 expression, decreased epididymal microvascularity and altered the morphometric features of the epididymal epithelium; no changes in AQP1 expression were observed at the beginning of postnatal epididymal development. Maternal protein restriction alters microvascularization and affects molecules involved in the epidydimal microenvironment, resulting in morphometric alterations related to a delay in the beginning of epididymis postnatal development. |
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Repositório Institucional da UNESP |
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Maternal Protein Restriction Modulates Angiogenesis and AQP9 Expression Leading to a Delay in Postnatal Epididymal Development in Rataqp1aqp9epididymal developmentfetal programingprotein restrictionvegfThe maternal nutritional status is essential to the health and well-being of the fetus. Maternal protein restriction during the perinatal stage causes sperm alterations in the offspring that are associated with epididymal dysfunctions. Vascular endothelial growth factor (VEGF) and its receptor, VEGFr-2, as well as aquaporins (AQPs) are important regulators of angiogenesis and the epididymal microenvironment and are associated with male fertility. We investigated the effects of maternal protein restriction on epididymal angiogenesis and AQP expression in the early stages of postnatal epididymal development. Pregnant rats were divided into two experimental groups that received either a normoprotein (17% protein) or low-protein diet (6% protein) during gestation and lactation. At postnatal day (PND)7 and PND14, male offspring were euthanized, the epididymides were subjected to morphometric and microvascular density analyses and to VEGF-A, VEGF-r2, AQP1 and AQP9 expression analyses. The maternal low-protein diet decreased AQP9 and VEGFr-2 expression, decreased epididymal microvascularity and altered the morphometric features of the epididymal epithelium; no changes in AQP1 expression were observed at the beginning of postnatal epididymal development. Maternal protein restriction alters microvascularization and affects molecules involved in the epidydimal microenvironment, resulting in morphometric alterations related to a delay in the beginning of epididymis postnatal development.Department of Anatomy Institute of Biosciences São Paulo State University-UNESPDepartment of Anatomy Institute of Biosciences São Paulo State University-UNESPUniversidade Estadual Paulista (Unesp)de Mello Santos, Talita [UNESP]Cavariani, Marilia Martins [UNESP]Pereira, Dhrielly Natália [UNESP]Schimming, Bruno César [UNESP]Chuffa, Luiz Gustavo de Almeida [UNESP]Domeniconi, Raquel Fantin [UNESP]2020-12-12T02:28:46Z2020-12-12T02:28:46Z2019-09-17info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3390/cells8091094Cells, v. 8, n. 9, 2019.2073-4409http://hdl.handle.net/11449/20128810.3390/cells80910942-s2.0-8507432968154817565282994690000-0003-2938-010XScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengCellsinfo:eu-repo/semantics/openAccess2021-10-22T16:05:39Zoai:repositorio.unesp.br:11449/201288Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-22T16:05:39Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Maternal Protein Restriction Modulates Angiogenesis and AQP9 Expression Leading to a Delay in Postnatal Epididymal Development in Rat |
title |
Maternal Protein Restriction Modulates Angiogenesis and AQP9 Expression Leading to a Delay in Postnatal Epididymal Development in Rat |
spellingShingle |
Maternal Protein Restriction Modulates Angiogenesis and AQP9 Expression Leading to a Delay in Postnatal Epididymal Development in Rat de Mello Santos, Talita [UNESP] aqp1 aqp9 epididymal development fetal programing protein restriction vegf |
title_short |
Maternal Protein Restriction Modulates Angiogenesis and AQP9 Expression Leading to a Delay in Postnatal Epididymal Development in Rat |
title_full |
Maternal Protein Restriction Modulates Angiogenesis and AQP9 Expression Leading to a Delay in Postnatal Epididymal Development in Rat |
title_fullStr |
Maternal Protein Restriction Modulates Angiogenesis and AQP9 Expression Leading to a Delay in Postnatal Epididymal Development in Rat |
title_full_unstemmed |
Maternal Protein Restriction Modulates Angiogenesis and AQP9 Expression Leading to a Delay in Postnatal Epididymal Development in Rat |
title_sort |
Maternal Protein Restriction Modulates Angiogenesis and AQP9 Expression Leading to a Delay in Postnatal Epididymal Development in Rat |
author |
de Mello Santos, Talita [UNESP] |
author_facet |
de Mello Santos, Talita [UNESP] Cavariani, Marilia Martins [UNESP] Pereira, Dhrielly Natália [UNESP] Schimming, Bruno César [UNESP] Chuffa, Luiz Gustavo de Almeida [UNESP] Domeniconi, Raquel Fantin [UNESP] |
author_role |
author |
author2 |
Cavariani, Marilia Martins [UNESP] Pereira, Dhrielly Natália [UNESP] Schimming, Bruno César [UNESP] Chuffa, Luiz Gustavo de Almeida [UNESP] Domeniconi, Raquel Fantin [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
de Mello Santos, Talita [UNESP] Cavariani, Marilia Martins [UNESP] Pereira, Dhrielly Natália [UNESP] Schimming, Bruno César [UNESP] Chuffa, Luiz Gustavo de Almeida [UNESP] Domeniconi, Raquel Fantin [UNESP] |
dc.subject.por.fl_str_mv |
aqp1 aqp9 epididymal development fetal programing protein restriction vegf |
topic |
aqp1 aqp9 epididymal development fetal programing protein restriction vegf |
description |
The maternal nutritional status is essential to the health and well-being of the fetus. Maternal protein restriction during the perinatal stage causes sperm alterations in the offspring that are associated with epididymal dysfunctions. Vascular endothelial growth factor (VEGF) and its receptor, VEGFr-2, as well as aquaporins (AQPs) are important regulators of angiogenesis and the epididymal microenvironment and are associated with male fertility. We investigated the effects of maternal protein restriction on epididymal angiogenesis and AQP expression in the early stages of postnatal epididymal development. Pregnant rats were divided into two experimental groups that received either a normoprotein (17% protein) or low-protein diet (6% protein) during gestation and lactation. At postnatal day (PND)7 and PND14, male offspring were euthanized, the epididymides were subjected to morphometric and microvascular density analyses and to VEGF-A, VEGF-r2, AQP1 and AQP9 expression analyses. The maternal low-protein diet decreased AQP9 and VEGFr-2 expression, decreased epididymal microvascularity and altered the morphometric features of the epididymal epithelium; no changes in AQP1 expression were observed at the beginning of postnatal epididymal development. Maternal protein restriction alters microvascularization and affects molecules involved in the epidydimal microenvironment, resulting in morphometric alterations related to a delay in the beginning of epididymis postnatal development. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-09-17 2020-12-12T02:28:46Z 2020-12-12T02:28:46Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3390/cells8091094 Cells, v. 8, n. 9, 2019. 2073-4409 http://hdl.handle.net/11449/201288 10.3390/cells8091094 2-s2.0-85074329681 5481756528299469 0000-0003-2938-010X |
url |
http://dx.doi.org/10.3390/cells8091094 http://hdl.handle.net/11449/201288 |
identifier_str_mv |
Cells, v. 8, n. 9, 2019. 2073-4409 10.3390/cells8091094 2-s2.0-85074329681 5481756528299469 0000-0003-2938-010X |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Cells |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1797789716875051008 |