Silver nanoparticles associated with a polyphosphate and fluoride enhance the prevention of enamel demineralization and impact on dual-biofilm adhesion
Main Author: | |
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Publication Date: | 2022 |
Other Authors: | , , , , , , , |
Format: | Article |
Language: | eng |
Source: | Repositório Institucional da UNESP |
Download full: | http://dx.doi.org/10.1016/j.jdent.2022.104245 http://hdl.handle.net/11449/242203 |
Summary: | Objectives: The aim of this study were to produce a multifunctional nanocomposite combining silver nanoaparticles (Ag), sodium trimetaphosphate (TMP) and fluoride (F), to investigate its effect on dental enamel demineralization and on biofilms of Streptococcus mutans and Candida albicans. Methods: Bovine enamel blocks were submitted to five pH cycles and treated 2x/day with 100 ppm F, 225 ppm F, 100 ppm F + 0.2%TMP or 100 ppm F + 0.2%TMP+10% Ag (100F/TMP/Ag). Next, surface hardness loss (%SH), integrated loss of subsurface hardness (ΔKHN), enamel fluoride (F) and calcium (Ca) concentration were determined. Biofilms from single and dual species of S. mutans and C. albicans were treated with 100F/TMP/Ag, Ag or chlorhexidine gluconate for 24 h. The antibiofilm effect was evaluated by colony-forming unit counting and Scanning Electron Microscopy. Results: The nanocomposite reduced 43.0% of %SH and was similar with samples treated with 225F, 100F/TMP and 100/TMP/Ag. The attribute of F and/or TMP in reducing ΔKHN in 5–20 μm was not affected by the addiction of Ag (110F = 225F = 100F/TMP = 100F/TMP/Ag > Negative Control). Further, 100F/TMP/Ag strongly reduced viable cells of S. mutans in dual biofilms (∼5 log10cm2) and structurally affected the biofilms. Conclusion: The 100F/TMP/F promoted a protective effect against enamel demineralization and was able to significantly inhibit the growth of biofilms of S. mutans and C. albicans. Clinical significance: The focus on prevention and non-invasive dental treatment is the most effective and least costly way to improve the population's oral health conditions. We present a nanocomposite for a multiple approach in prevention of caries. |
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Silver nanoparticles associated with a polyphosphate and fluoride enhance the prevention of enamel demineralization and impact on dual-biofilm adhesionDental cariesFluorideNanoparticlesPolyphosphatesSilver CompoundsObjectives: The aim of this study were to produce a multifunctional nanocomposite combining silver nanoaparticles (Ag), sodium trimetaphosphate (TMP) and fluoride (F), to investigate its effect on dental enamel demineralization and on biofilms of Streptococcus mutans and Candida albicans. Methods: Bovine enamel blocks were submitted to five pH cycles and treated 2x/day with 100 ppm F, 225 ppm F, 100 ppm F + 0.2%TMP or 100 ppm F + 0.2%TMP+10% Ag (100F/TMP/Ag). Next, surface hardness loss (%SH), integrated loss of subsurface hardness (ΔKHN), enamel fluoride (F) and calcium (Ca) concentration were determined. Biofilms from single and dual species of S. mutans and C. albicans were treated with 100F/TMP/Ag, Ag or chlorhexidine gluconate for 24 h. The antibiofilm effect was evaluated by colony-forming unit counting and Scanning Electron Microscopy. Results: The nanocomposite reduced 43.0% of %SH and was similar with samples treated with 225F, 100F/TMP and 100/TMP/Ag. The attribute of F and/or TMP in reducing ΔKHN in 5–20 μm was not affected by the addiction of Ag (110F = 225F = 100F/TMP = 100F/TMP/Ag > Negative Control). Further, 100F/TMP/Ag strongly reduced viable cells of S. mutans in dual biofilms (∼5 log10cm2) and structurally affected the biofilms. Conclusion: The 100F/TMP/F promoted a protective effect against enamel demineralization and was able to significantly inhibit the growth of biofilms of S. mutans and C. albicans. Clinical significance: The focus on prevention and non-invasive dental treatment is the most effective and least costly way to improve the population's oral health conditions. We present a nanocomposite for a multiple approach in prevention of caries.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Graduate Program of Dental Science School of Dentistry São Paulo State University (UNESP), São PauloSchool of Dentistry University of Ribeirão Preto - UNAERP, São PauloDepartment of Restorative Dentistry School of Dentistry São Paulo State University (UNESP), São PauloInstitute of Chemistry Federal University of Alfenas, Minas GeraisDepartment of Chemistry Federal University of São Carlos, São PauloDepartment of Dental Materials and Prosthodontics School of Dentistry São Paulo State University (UNESP), São PauloGraduate Program of Dental Science School of Dentistry São Paulo State University (UNESP), São PauloDepartment of Restorative Dentistry School of Dentistry São Paulo State University (UNESP), São PauloDepartment of Dental Materials and Prosthodontics School of Dentistry São Paulo State University (UNESP), São PauloCAPES: 001Universidade Estadual Paulista (UNESP)University of Ribeirão Preto - UNAERPFederal University of AlfenasUniversidade Federal de São Carlos (UFSCar)Mendes-Gouvêa, Carla Corrêa [UNESP]Danelon, Marcelle [UNESP]Vieira, Ana Paula Miranda [UNESP]do Amaral, Jackeline Gallo [UNESP]de Souza Neto, Francisco Nunes [UNESP]Gorup, Luiz FernandoCamargo, Emerson RodriguesDelbem, Alberto Carlos Botazzo [UNESP]Barbosa, Debora Barros [UNESP]2023-03-02T11:50:58Z2023-03-02T11:50:58Z2022-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.jdent.2022.104245Journal of Dentistry, v. 125.0300-5712http://hdl.handle.net/11449/24220310.1016/j.jdent.2022.1042452-s2.0-85136712235Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Dentistryinfo:eu-repo/semantics/openAccess2023-03-02T11:50:58Zoai:repositorio.unesp.br:11449/242203Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-03-02T11:50:58Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Silver nanoparticles associated with a polyphosphate and fluoride enhance the prevention of enamel demineralization and impact on dual-biofilm adhesion |
title |
Silver nanoparticles associated with a polyphosphate and fluoride enhance the prevention of enamel demineralization and impact on dual-biofilm adhesion |
spellingShingle |
Silver nanoparticles associated with a polyphosphate and fluoride enhance the prevention of enamel demineralization and impact on dual-biofilm adhesion Mendes-Gouvêa, Carla Corrêa [UNESP] Dental caries Fluoride Nanoparticles Polyphosphates Silver Compounds |
title_short |
Silver nanoparticles associated with a polyphosphate and fluoride enhance the prevention of enamel demineralization and impact on dual-biofilm adhesion |
title_full |
Silver nanoparticles associated with a polyphosphate and fluoride enhance the prevention of enamel demineralization and impact on dual-biofilm adhesion |
title_fullStr |
Silver nanoparticles associated with a polyphosphate and fluoride enhance the prevention of enamel demineralization and impact on dual-biofilm adhesion |
title_full_unstemmed |
Silver nanoparticles associated with a polyphosphate and fluoride enhance the prevention of enamel demineralization and impact on dual-biofilm adhesion |
title_sort |
Silver nanoparticles associated with a polyphosphate and fluoride enhance the prevention of enamel demineralization and impact on dual-biofilm adhesion |
author |
Mendes-Gouvêa, Carla Corrêa [UNESP] |
author_facet |
Mendes-Gouvêa, Carla Corrêa [UNESP] Danelon, Marcelle [UNESP] Vieira, Ana Paula Miranda [UNESP] do Amaral, Jackeline Gallo [UNESP] de Souza Neto, Francisco Nunes [UNESP] Gorup, Luiz Fernando Camargo, Emerson Rodrigues Delbem, Alberto Carlos Botazzo [UNESP] Barbosa, Debora Barros [UNESP] |
author_role |
author |
author2 |
Danelon, Marcelle [UNESP] Vieira, Ana Paula Miranda [UNESP] do Amaral, Jackeline Gallo [UNESP] de Souza Neto, Francisco Nunes [UNESP] Gorup, Luiz Fernando Camargo, Emerson Rodrigues Delbem, Alberto Carlos Botazzo [UNESP] Barbosa, Debora Barros [UNESP] |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) University of Ribeirão Preto - UNAERP Federal University of Alfenas Universidade Federal de São Carlos (UFSCar) |
dc.contributor.author.fl_str_mv |
Mendes-Gouvêa, Carla Corrêa [UNESP] Danelon, Marcelle [UNESP] Vieira, Ana Paula Miranda [UNESP] do Amaral, Jackeline Gallo [UNESP] de Souza Neto, Francisco Nunes [UNESP] Gorup, Luiz Fernando Camargo, Emerson Rodrigues Delbem, Alberto Carlos Botazzo [UNESP] Barbosa, Debora Barros [UNESP] |
dc.subject.por.fl_str_mv |
Dental caries Fluoride Nanoparticles Polyphosphates Silver Compounds |
topic |
Dental caries Fluoride Nanoparticles Polyphosphates Silver Compounds |
description |
Objectives: The aim of this study were to produce a multifunctional nanocomposite combining silver nanoaparticles (Ag), sodium trimetaphosphate (TMP) and fluoride (F), to investigate its effect on dental enamel demineralization and on biofilms of Streptococcus mutans and Candida albicans. Methods: Bovine enamel blocks were submitted to five pH cycles and treated 2x/day with 100 ppm F, 225 ppm F, 100 ppm F + 0.2%TMP or 100 ppm F + 0.2%TMP+10% Ag (100F/TMP/Ag). Next, surface hardness loss (%SH), integrated loss of subsurface hardness (ΔKHN), enamel fluoride (F) and calcium (Ca) concentration were determined. Biofilms from single and dual species of S. mutans and C. albicans were treated with 100F/TMP/Ag, Ag or chlorhexidine gluconate for 24 h. The antibiofilm effect was evaluated by colony-forming unit counting and Scanning Electron Microscopy. Results: The nanocomposite reduced 43.0% of %SH and was similar with samples treated with 225F, 100F/TMP and 100/TMP/Ag. The attribute of F and/or TMP in reducing ΔKHN in 5–20 μm was not affected by the addiction of Ag (110F = 225F = 100F/TMP = 100F/TMP/Ag > Negative Control). Further, 100F/TMP/Ag strongly reduced viable cells of S. mutans in dual biofilms (∼5 log10cm2) and structurally affected the biofilms. Conclusion: The 100F/TMP/F promoted a protective effect against enamel demineralization and was able to significantly inhibit the growth of biofilms of S. mutans and C. albicans. Clinical significance: The focus on prevention and non-invasive dental treatment is the most effective and least costly way to improve the population's oral health conditions. We present a nanocomposite for a multiple approach in prevention of caries. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-10-01 2023-03-02T11:50:58Z 2023-03-02T11:50:58Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.jdent.2022.104245 Journal of Dentistry, v. 125. 0300-5712 http://hdl.handle.net/11449/242203 10.1016/j.jdent.2022.104245 2-s2.0-85136712235 |
url |
http://dx.doi.org/10.1016/j.jdent.2022.104245 http://hdl.handle.net/11449/242203 |
identifier_str_mv |
Journal of Dentistry, v. 125. 0300-5712 10.1016/j.jdent.2022.104245 2-s2.0-85136712235 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Dentistry |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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