Effects of resistance exercise training on bone physical and mineral properties in rats submitted to growth hormone intervention
Autor(a) principal: | |
---|---|
Data de Publicação: | 2023 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.vibspec.2022.103489 http://hdl.handle.net/11449/249507 |
Resumo: | Purpose: To investigate the effects of applying growth hormone (GH) and performing a strength training protocol on mineralization, rigidity, and crystallinity on the bone tissue of Wistar rats. Method: In total, 40 male animals, 60 days old, of the Wistar lineage were used, distributed into four groups: control (C) [n = 10], control and application of GH (GHC) [n = 10], resistance training (T) [n = 10], and resistance training and application of GH (GHT) [n = 10]. The training period lasted four weeks and consisted of four sets of 10 jumps in a liquid medium. The amount of 0.2 IU per kilogram of body mass (0.2 IU/Kg) of GH was administered in the GHC and GHT groups. After the experimental period, samples of the tibia were collected and analyzed using Raman Spectroscopy (633 nm), Bone Densitometry - dual-energy x-ray absorptiometry (DXA), and Mechanical Assay (F-max). Results: The GHT group presented an increase for the variable Amide II (1544 cm-1) and decrease for the variable Proline (727 cm-1) from the Raman Spectroscopy analysis (p < 0.05). In addition, an increase was observed for the variables bone mineral density (BMD) and bone mineral content (BMC) (p < 0.05). Finally, no statistically significant differences were observed for the variables mineralization, crystallinity, phosphate carbonate types A and B, and bone rigidity (p > 0.05). Conclusion: It is concluded that GH added to RT resulted in an increase in the mineral Amide II (1544 cm-1) and a decrease in Proline (727 cm-1). Furthermore, an increase in BMD and BMC was observed only in the GHT group. Finally, no alterations were observed in crystallinity and rigidity in the different groups of animals. |
id |
UNSP_e4fcfbc39e5a831c993309daf8a1607a |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/249507 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Effects of resistance exercise training on bone physical and mineral properties in rats submitted to growth hormone interventionBone tissueMineralsPhysical trainingRaman SpectroscopySomatropinPurpose: To investigate the effects of applying growth hormone (GH) and performing a strength training protocol on mineralization, rigidity, and crystallinity on the bone tissue of Wistar rats. Method: In total, 40 male animals, 60 days old, of the Wistar lineage were used, distributed into four groups: control (C) [n = 10], control and application of GH (GHC) [n = 10], resistance training (T) [n = 10], and resistance training and application of GH (GHT) [n = 10]. The training period lasted four weeks and consisted of four sets of 10 jumps in a liquid medium. The amount of 0.2 IU per kilogram of body mass (0.2 IU/Kg) of GH was administered in the GHC and GHT groups. After the experimental period, samples of the tibia were collected and analyzed using Raman Spectroscopy (633 nm), Bone Densitometry - dual-energy x-ray absorptiometry (DXA), and Mechanical Assay (F-max). Results: The GHT group presented an increase for the variable Amide II (1544 cm-1) and decrease for the variable Proline (727 cm-1) from the Raman Spectroscopy analysis (p < 0.05). In addition, an increase was observed for the variables bone mineral density (BMD) and bone mineral content (BMC) (p < 0.05). Finally, no statistically significant differences were observed for the variables mineralization, crystallinity, phosphate carbonate types A and B, and bone rigidity (p > 0.05). Conclusion: It is concluded that GH added to RT resulted in an increase in the mineral Amide II (1544 cm-1) and a decrease in Proline (727 cm-1). Furthermore, an increase in BMD and BMC was observed only in the GHT group. Finally, no alterations were observed in crystallinity and rigidity in the different groups of animals.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Department of Physical Therapy Universidade Estadual Paulista “Júlio de Mesquita Filho” – UNESP, State of São PauloFaculty of Veterinary Medicine Universidade do Oeste Paulista – UNOESTE, State of São PauloDepartment of Physics Chemistry and Biology Universidade Estadual Paulista “Júlio de Mesquita Filho” – UNESP, State of São PauloPostgraduate Program in Movement Sciences Federal University of Mato Grosso do Sul – UFMSCatholic Salesian Auxilium University Centre, São PauloFaculty of Medical Sciences State University of Campinas – UNICAMP, São Paulo StateDepartment of Physical Therapy Universidade Estadual Paulista “Júlio de Mesquita Filho” – UNESP, State of São PauloDepartment of Physics Chemistry and Biology Universidade Estadual Paulista “Júlio de Mesquita Filho” – UNESP, State of São PauloFAPESP: 2018/22214-6CNPq: 2020/05423-0Universidade Estadual Paulista (UNESP)Universidade do Oeste Paulista – UNOESTEUniversidade Federal de Mato Grosso do Sul (UFMS)Catholic Salesian Auxilium University CentreUniversidade Estadual de Campinas (UNICAMP)Castoldi, Robson Chacon [UNESP]Giometti, Ines CristinaOzaki, Guilherme Akio Tamura [UNESP]Garcia, Thiago Alves [UNESP]dos Santos Pereira, João Domingos Augusto [UNESP]Rubira, Rafael Jesus Gonçalves [UNESP]Constantino, Carlos José Leopoldo [UNESP]de Oliveira Júnior, Silvio AssisLouzada, Mario Jefferson QuirinoBelangero, William DiasSilva Camargo Filho, José Carlos [UNESP]2023-07-29T16:01:31Z2023-07-29T16:01:31Z2023-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.vibspec.2022.103489Vibrational Spectroscopy, v. 124.0924-2031http://hdl.handle.net/11449/24950710.1016/j.vibspec.2022.1034892-s2.0-85144824666Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengVibrational Spectroscopyinfo:eu-repo/semantics/openAccess2023-07-29T16:01:31Zoai:repositorio.unesp.br:11449/249507Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-07-29T16:01:31Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Effects of resistance exercise training on bone physical and mineral properties in rats submitted to growth hormone intervention |
title |
Effects of resistance exercise training on bone physical and mineral properties in rats submitted to growth hormone intervention |
spellingShingle |
Effects of resistance exercise training on bone physical and mineral properties in rats submitted to growth hormone intervention Castoldi, Robson Chacon [UNESP] Bone tissue Minerals Physical training Raman Spectroscopy Somatropin |
title_short |
Effects of resistance exercise training on bone physical and mineral properties in rats submitted to growth hormone intervention |
title_full |
Effects of resistance exercise training on bone physical and mineral properties in rats submitted to growth hormone intervention |
title_fullStr |
Effects of resistance exercise training on bone physical and mineral properties in rats submitted to growth hormone intervention |
title_full_unstemmed |
Effects of resistance exercise training on bone physical and mineral properties in rats submitted to growth hormone intervention |
title_sort |
Effects of resistance exercise training on bone physical and mineral properties in rats submitted to growth hormone intervention |
author |
Castoldi, Robson Chacon [UNESP] |
author_facet |
Castoldi, Robson Chacon [UNESP] Giometti, Ines Cristina Ozaki, Guilherme Akio Tamura [UNESP] Garcia, Thiago Alves [UNESP] dos Santos Pereira, João Domingos Augusto [UNESP] Rubira, Rafael Jesus Gonçalves [UNESP] Constantino, Carlos José Leopoldo [UNESP] de Oliveira Júnior, Silvio Assis Louzada, Mario Jefferson Quirino Belangero, William Dias Silva Camargo Filho, José Carlos [UNESP] |
author_role |
author |
author2 |
Giometti, Ines Cristina Ozaki, Guilherme Akio Tamura [UNESP] Garcia, Thiago Alves [UNESP] dos Santos Pereira, João Domingos Augusto [UNESP] Rubira, Rafael Jesus Gonçalves [UNESP] Constantino, Carlos José Leopoldo [UNESP] de Oliveira Júnior, Silvio Assis Louzada, Mario Jefferson Quirino Belangero, William Dias Silva Camargo Filho, José Carlos [UNESP] |
author2_role |
author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Universidade do Oeste Paulista – UNOESTE Universidade Federal de Mato Grosso do Sul (UFMS) Catholic Salesian Auxilium University Centre Universidade Estadual de Campinas (UNICAMP) |
dc.contributor.author.fl_str_mv |
Castoldi, Robson Chacon [UNESP] Giometti, Ines Cristina Ozaki, Guilherme Akio Tamura [UNESP] Garcia, Thiago Alves [UNESP] dos Santos Pereira, João Domingos Augusto [UNESP] Rubira, Rafael Jesus Gonçalves [UNESP] Constantino, Carlos José Leopoldo [UNESP] de Oliveira Júnior, Silvio Assis Louzada, Mario Jefferson Quirino Belangero, William Dias Silva Camargo Filho, José Carlos [UNESP] |
dc.subject.por.fl_str_mv |
Bone tissue Minerals Physical training Raman Spectroscopy Somatropin |
topic |
Bone tissue Minerals Physical training Raman Spectroscopy Somatropin |
description |
Purpose: To investigate the effects of applying growth hormone (GH) and performing a strength training protocol on mineralization, rigidity, and crystallinity on the bone tissue of Wistar rats. Method: In total, 40 male animals, 60 days old, of the Wistar lineage were used, distributed into four groups: control (C) [n = 10], control and application of GH (GHC) [n = 10], resistance training (T) [n = 10], and resistance training and application of GH (GHT) [n = 10]. The training period lasted four weeks and consisted of four sets of 10 jumps in a liquid medium. The amount of 0.2 IU per kilogram of body mass (0.2 IU/Kg) of GH was administered in the GHC and GHT groups. After the experimental period, samples of the tibia were collected and analyzed using Raman Spectroscopy (633 nm), Bone Densitometry - dual-energy x-ray absorptiometry (DXA), and Mechanical Assay (F-max). Results: The GHT group presented an increase for the variable Amide II (1544 cm-1) and decrease for the variable Proline (727 cm-1) from the Raman Spectroscopy analysis (p < 0.05). In addition, an increase was observed for the variables bone mineral density (BMD) and bone mineral content (BMC) (p < 0.05). Finally, no statistically significant differences were observed for the variables mineralization, crystallinity, phosphate carbonate types A and B, and bone rigidity (p > 0.05). Conclusion: It is concluded that GH added to RT resulted in an increase in the mineral Amide II (1544 cm-1) and a decrease in Proline (727 cm-1). Furthermore, an increase in BMD and BMC was observed only in the GHT group. Finally, no alterations were observed in crystallinity and rigidity in the different groups of animals. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-07-29T16:01:31Z 2023-07-29T16:01:31Z 2023-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.vibspec.2022.103489 Vibrational Spectroscopy, v. 124. 0924-2031 http://hdl.handle.net/11449/249507 10.1016/j.vibspec.2022.103489 2-s2.0-85144824666 |
url |
http://dx.doi.org/10.1016/j.vibspec.2022.103489 http://hdl.handle.net/11449/249507 |
identifier_str_mv |
Vibrational Spectroscopy, v. 124. 0924-2031 10.1016/j.vibspec.2022.103489 2-s2.0-85144824666 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Vibrational Spectroscopy |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965063139819520 |