EXPRESSÃO DIFERENCIAL DO RECEPTOR DE LH, DA PROTEÍNA DE LIGAÇÃO DE MRNA DO LHR, BTA-MIR-222 E ENZIMAS ESTEROIDOGÊNICAS NO OVÁRIO BOVINO EM DESENVOLVIMENTO
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da UNOESTE |
Texto Completo: | http://bdtd.unoeste.br:8080/jspui/handle/jspui/1117 |
Resumo: | Steroids and gonadotrophins are essential for the regulation of antral follicular development and the late stages of preantral development. Although the luteinizing hormone receptor (LHR) has been detected in the preantral follicles of rats, rabbits, and pigs, the expression of this receptor in bovine fetal ovary has not been demonstrated. The present study aimed to quantify the expression of the LHR and the mRNA abundance of the genes LHR binding protein (LRBP), STAR, HSD3B1, CYP17A1, and CYP19A1 during the development of bovine fetal ovary. In addition, we aimed to identify and quantify the expression of bta-miR-222 (a regulatory microRNA of the LHCGR gene). In summary, LHR expression was observed in the preantral follicle in bovine fetal ovary, from oogonias to primordial, primary and secondary stages, and the mRNA abundance was lower on day 150 than day 60. However, the mRNA abundance of LRBP followed the opposite pattern. The LHR protein was detected in oogonia, primordial, primary, and secondary follicles. Moreover, both oocytes and granulosa cells showed positive immunostaining for LHR. Similar to LRBP, the abundance of bta-miR-222 was higher on day 150 than day 60 or 90 of gestation. With regard to the gene expression of steroidogenic enzymes; only the mRNA abundance of STAR was higher on day 150 than on day 60. In conclusion, these results suggested the involvement of LHCGR/LRBP regulation with mechanisms related to the development of preantral follicles, especially during the establishment of secondary follicles. Furthermore, the present data reinforced that the reduced expression of LHR mRNA in bovine fetal ovaries on day 150 was related to the higher expression of LRBP and bta-miR-222. |
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Castilho, Anthony César de Souzahttp://lattes.cnpq.br/0480944334194184Chuffa, Luiz Gustavo de Almeidahttp://lattes.cnpq.br/5121319676503034Giometti, Inês Cristinahttp://lattes.cnpq.br/546121613709436835334036881http://lattes.cnpq.br/580245674597346Chaves, Marina Platzeck2018-11-30T12:45:47Z2018-05-30Chaves, Marina Platzeck. EXPRESSÃO DIFERENCIAL DO RECEPTOR DE LH, DA PROTEÍNA DE LIGAÇÃO DE MRNA DO LHR, BTA-MIR-222 E ENZIMAS ESTEROIDOGÊNICAS NO OVÁRIO BOVINO EM DESENVOLVIMENTO. 2018. 40f. Dissertação (Mestrado em Ciência Animal) - Universidade do Oeste Paulista, Presidente Prudente, 2018.http://bdtd.unoeste.br:8080/jspui/handle/jspui/1117Steroids and gonadotrophins are essential for the regulation of antral follicular development and the late stages of preantral development. Although the luteinizing hormone receptor (LHR) has been detected in the preantral follicles of rats, rabbits, and pigs, the expression of this receptor in bovine fetal ovary has not been demonstrated. The present study aimed to quantify the expression of the LHR and the mRNA abundance of the genes LHR binding protein (LRBP), STAR, HSD3B1, CYP17A1, and CYP19A1 during the development of bovine fetal ovary. In addition, we aimed to identify and quantify the expression of bta-miR-222 (a regulatory microRNA of the LHCGR gene). In summary, LHR expression was observed in the preantral follicle in bovine fetal ovary, from oogonias to primordial, primary and secondary stages, and the mRNA abundance was lower on day 150 than day 60. However, the mRNA abundance of LRBP followed the opposite pattern. The LHR protein was detected in oogonia, primordial, primary, and secondary follicles. Moreover, both oocytes and granulosa cells showed positive immunostaining for LHR. Similar to LRBP, the abundance of bta-miR-222 was higher on day 150 than day 60 or 90 of gestation. With regard to the gene expression of steroidogenic enzymes; only the mRNA abundance of STAR was higher on day 150 than on day 60. In conclusion, these results suggested the involvement of LHCGR/LRBP regulation with mechanisms related to the development of preantral follicles, especially during the establishment of secondary follicles. Furthermore, the present data reinforced that the reduced expression of LHR mRNA in bovine fetal ovaries on day 150 was related to the higher expression of LRBP and bta-miR-222.Esteroides e gonadotrofinas são essenciais para a regulação do desenvolvimento folicular antral e os estágios finais do desenvolvimento pré-antral. Embora o receptor do hormônio luteinizante (LHR) tenha sido detectado nos folículos pré-antrais de ratos, coelhos e porcos, a expressão deste receptor no ovário fetal bovino não foi demonstrada. O presente estudo teve como objetivo quantificar a expressão do LHR e a abundância de mRNA da proteína de ligação LHR (LRBP), STAR, HSD3B1, CYP17A1 e CYP19A1 durante o desenvolvimento do ovário fetal bovino. Além disso, objetivamos identificar e quantificar a expressão de bta-miR-222 (microRNA regulador do gene LHCGR). Em resumo, a expressão de LHR foi observada no folículo pré-antral no ovário fetal de bovino e a abundância de mRNA foi menor no dia 150 do que no dia 60. No entanto, a abundância de mRNA da LRBP seguiu o padrão oposto. Semelhante a LRBP, a abundância de bta-miR-222 foi maior no dia 150 do que no dia 60 ou 90. Com relação à expressão gênica de enzimas esteroidogênicas; apenas a abundância de mRNA de STAR foi maior no dia 150 do que no dia 60. A proteína LHR foi detectada em oogônia, folículos primordiais, primários e secundários. Além disso, ambos os oócitos e células da granulosa apresentaram imunolocalização positiva para LHR. Em conclusão, estes resultados sugeriram o envolvimento da regulação do LHCGR / LBPB com mecanismos relacionados ao desenvolvimento de folículos pré-antrais, especialmente durante o estabelecimento de folículos secundários. Além disso, os presentes dados reforçaram que a expressão reduzida de mRNA de LHR em ovários fetais bovinos no dia 150 estava relacionada à maior expressão de LRBP e bta-miR-222.Submitted by Michele Mologni (mologni@unoeste.br) on 2018-11-30T12:45:47Z No. of bitstreams: 1 Marina Platzeck Chaves.pdf: 891914 bytes, checksum: 43d527b64ad46e92f25496042406c5e9 (MD5)Made available in DSpace on 2018-11-30T12:45:47Z (GMT). No. of bitstreams: 1 Marina Platzeck Chaves.pdf: 891914 bytes, checksum: 43d527b64ad46e92f25496042406c5e9 (MD5) Previous issue date: 2018-05-30application/pdfhttp://bdtd.unoeste.br:8080/jspui/retrieve/2879/Marina%20Platzeck%20Chaves.pdf.jpgporUniversidade do Oeste PaulistaMestrado em Ciência AnimalUNOESTEBrasilMestrado em Ciência AnimalLHCGR, mevalonato quinase, folículo pré-antral, esteroidogênese, microRNAsLHCGR, mevalonate kinase, preantral follicle, steroidogenesis, microRNAsCIENCIAS AGRARIAS::MEDICINA VETERINARIAEXPRESSÃO DIFERENCIAL DO RECEPTOR DE LH, DA PROTEÍNA DE LIGAÇÃO DE MRNA DO LHR, BTA-MIR-222 E ENZIMAS ESTEROIDOGÊNICAS NO OVÁRIO BOVINO EM DESENVOLVIMENTODIFFERENTIAL EXPRESSION OF LH RECEPTOR, LHR MRNA BINDING PROTEIN, BTA-MIR-222 AND STEROIDOGENIC ENZYMES IN THE DEVELOPING BOVINE OVARYinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis18867256397436141355005006001886725639743614135453670264235017319info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UNOESTEinstname:Universidade do Oeste Paulista (UNOESTE)instacron:UNOESTELICENSElicense.txtlicense.txttext/plain; charset=utf-82067http://bdtd.unoeste.br:8080/tede/bitstream/jspui/1117/1/license.txt47745281809acb27fb322a97f2d9cb88MD51ORIGINALMarina Platzeck Chaves.pdfMarina Platzeck Chaves.pdfapplication/pdf891914http://bdtd.unoeste.br:8080/tede/bitstream/jspui/1117/2/Marina+Platzeck+Chaves.pdf43d527b64ad46e92f25496042406c5e9MD52THUMBNAILMarina Platzeck Chaves.pdf.jpgMarina Platzeck Chaves.pdf.jpgimage/jpeg2156http://bdtd.unoeste.br:8080/tede/bitstream/jspui/1117/3/Marina+Platzeck+Chaves.pdf.jpg658644fa4315f92afb433b0f56234055MD53jspui/11172018-12-01 01:00:11.54oai:bdtd.unoeste.br: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 Digital de Teses e Dissertaçõeshttp://bdtd.unoeste.br:8080/jspui/PUBhttp://bdtd.unoeste.br:8080/oai/requestbdtd@unoeste.bropendoar:2018-12-01T03:00:11Biblioteca Digital de Teses e Dissertações da UNOESTE - Universidade do Oeste Paulista (UNOESTE)false |
dc.title.por.fl_str_mv |
EXPRESSÃO DIFERENCIAL DO RECEPTOR DE LH, DA PROTEÍNA DE LIGAÇÃO DE MRNA DO LHR, BTA-MIR-222 E ENZIMAS ESTEROIDOGÊNICAS NO OVÁRIO BOVINO EM DESENVOLVIMENTO |
dc.title.alternative.eng.fl_str_mv |
DIFFERENTIAL EXPRESSION OF LH RECEPTOR, LHR MRNA BINDING PROTEIN, BTA-MIR-222 AND STEROIDOGENIC ENZYMES IN THE DEVELOPING BOVINE OVARY |
title |
EXPRESSÃO DIFERENCIAL DO RECEPTOR DE LH, DA PROTEÍNA DE LIGAÇÃO DE MRNA DO LHR, BTA-MIR-222 E ENZIMAS ESTEROIDOGÊNICAS NO OVÁRIO BOVINO EM DESENVOLVIMENTO |
spellingShingle |
EXPRESSÃO DIFERENCIAL DO RECEPTOR DE LH, DA PROTEÍNA DE LIGAÇÃO DE MRNA DO LHR, BTA-MIR-222 E ENZIMAS ESTEROIDOGÊNICAS NO OVÁRIO BOVINO EM DESENVOLVIMENTO Chaves, Marina Platzeck LHCGR, mevalonato quinase, folículo pré-antral, esteroidogênese, microRNAs LHCGR, mevalonate kinase, preantral follicle, steroidogenesis, microRNAs CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
title_short |
EXPRESSÃO DIFERENCIAL DO RECEPTOR DE LH, DA PROTEÍNA DE LIGAÇÃO DE MRNA DO LHR, BTA-MIR-222 E ENZIMAS ESTEROIDOGÊNICAS NO OVÁRIO BOVINO EM DESENVOLVIMENTO |
title_full |
EXPRESSÃO DIFERENCIAL DO RECEPTOR DE LH, DA PROTEÍNA DE LIGAÇÃO DE MRNA DO LHR, BTA-MIR-222 E ENZIMAS ESTEROIDOGÊNICAS NO OVÁRIO BOVINO EM DESENVOLVIMENTO |
title_fullStr |
EXPRESSÃO DIFERENCIAL DO RECEPTOR DE LH, DA PROTEÍNA DE LIGAÇÃO DE MRNA DO LHR, BTA-MIR-222 E ENZIMAS ESTEROIDOGÊNICAS NO OVÁRIO BOVINO EM DESENVOLVIMENTO |
title_full_unstemmed |
EXPRESSÃO DIFERENCIAL DO RECEPTOR DE LH, DA PROTEÍNA DE LIGAÇÃO DE MRNA DO LHR, BTA-MIR-222 E ENZIMAS ESTEROIDOGÊNICAS NO OVÁRIO BOVINO EM DESENVOLVIMENTO |
title_sort |
EXPRESSÃO DIFERENCIAL DO RECEPTOR DE LH, DA PROTEÍNA DE LIGAÇÃO DE MRNA DO LHR, BTA-MIR-222 E ENZIMAS ESTEROIDOGÊNICAS NO OVÁRIO BOVINO EM DESENVOLVIMENTO |
author |
Chaves, Marina Platzeck |
author_facet |
Chaves, Marina Platzeck |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Castilho, Anthony César de Souza |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/0480944334194184 |
dc.contributor.referee1.fl_str_mv |
Chuffa, Luiz Gustavo de Almeida |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/5121319676503034 |
dc.contributor.referee2.fl_str_mv |
Giometti, Inês Cristina |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/5461216137094368 |
dc.contributor.authorID.fl_str_mv |
35334036881 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/580245674597346 |
dc.contributor.author.fl_str_mv |
Chaves, Marina Platzeck |
contributor_str_mv |
Castilho, Anthony César de Souza Chuffa, Luiz Gustavo de Almeida Giometti, Inês Cristina |
dc.subject.por.fl_str_mv |
LHCGR, mevalonato quinase, folículo pré-antral, esteroidogênese, microRNAs |
topic |
LHCGR, mevalonato quinase, folículo pré-antral, esteroidogênese, microRNAs LHCGR, mevalonate kinase, preantral follicle, steroidogenesis, microRNAs CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
dc.subject.eng.fl_str_mv |
LHCGR, mevalonate kinase, preantral follicle, steroidogenesis, microRNAs |
dc.subject.cnpq.fl_str_mv |
CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
description |
Steroids and gonadotrophins are essential for the regulation of antral follicular development and the late stages of preantral development. Although the luteinizing hormone receptor (LHR) has been detected in the preantral follicles of rats, rabbits, and pigs, the expression of this receptor in bovine fetal ovary has not been demonstrated. The present study aimed to quantify the expression of the LHR and the mRNA abundance of the genes LHR binding protein (LRBP), STAR, HSD3B1, CYP17A1, and CYP19A1 during the development of bovine fetal ovary. In addition, we aimed to identify and quantify the expression of bta-miR-222 (a regulatory microRNA of the LHCGR gene). In summary, LHR expression was observed in the preantral follicle in bovine fetal ovary, from oogonias to primordial, primary and secondary stages, and the mRNA abundance was lower on day 150 than day 60. However, the mRNA abundance of LRBP followed the opposite pattern. The LHR protein was detected in oogonia, primordial, primary, and secondary follicles. Moreover, both oocytes and granulosa cells showed positive immunostaining for LHR. Similar to LRBP, the abundance of bta-miR-222 was higher on day 150 than day 60 or 90 of gestation. With regard to the gene expression of steroidogenic enzymes; only the mRNA abundance of STAR was higher on day 150 than on day 60. In conclusion, these results suggested the involvement of LHCGR/LRBP regulation with mechanisms related to the development of preantral follicles, especially during the establishment of secondary follicles. Furthermore, the present data reinforced that the reduced expression of LHR mRNA in bovine fetal ovaries on day 150 was related to the higher expression of LRBP and bta-miR-222. |
publishDate |
2018 |
dc.date.accessioned.fl_str_mv |
2018-11-30T12:45:47Z |
dc.date.issued.fl_str_mv |
2018-05-30 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Chaves, Marina Platzeck. EXPRESSÃO DIFERENCIAL DO RECEPTOR DE LH, DA PROTEÍNA DE LIGAÇÃO DE MRNA DO LHR, BTA-MIR-222 E ENZIMAS ESTEROIDOGÊNICAS NO OVÁRIO BOVINO EM DESENVOLVIMENTO. 2018. 40f. Dissertação (Mestrado em Ciência Animal) - Universidade do Oeste Paulista, Presidente Prudente, 2018. |
dc.identifier.uri.fl_str_mv |
http://bdtd.unoeste.br:8080/jspui/handle/jspui/1117 |
identifier_str_mv |
Chaves, Marina Platzeck. EXPRESSÃO DIFERENCIAL DO RECEPTOR DE LH, DA PROTEÍNA DE LIGAÇÃO DE MRNA DO LHR, BTA-MIR-222 E ENZIMAS ESTEROIDOGÊNICAS NO OVÁRIO BOVINO EM DESENVOLVIMENTO. 2018. 40f. Dissertação (Mestrado em Ciência Animal) - Universidade do Oeste Paulista, Presidente Prudente, 2018. |
url |
http://bdtd.unoeste.br:8080/jspui/handle/jspui/1117 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.program.fl_str_mv |
1886725639743614135 |
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500 500 600 |
dc.relation.department.fl_str_mv |
1886725639743614135 |
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453670264235017319 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
dc.publisher.none.fl_str_mv |
Universidade do Oeste Paulista |
dc.publisher.program.fl_str_mv |
Mestrado em Ciência Animal |
dc.publisher.initials.fl_str_mv |
UNOESTE |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Mestrado em Ciência Animal |
publisher.none.fl_str_mv |
Universidade do Oeste Paulista |
dc.source.none.fl_str_mv |
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