Estudo in vitro da interação entre herbicidas e espermatozoide humano utilizando a Espectroscopia de Espalhamento Raman
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da UNOESTE |
Texto Completo: | http://bdtd.unoeste.br:8080/tede/handle/tede/493 |
Resumo: | The unlimited use of pesticides, besides contribute with to increase environmental pollution, is also influencing negatively the health of the living beings, including the people health. Many studies show the result these chemicals in the reproductive system of animals and even same at man, can cause infertility problem, which can originate, among other factors, the low sperm quality and quantity. This is study aimed at evaluating human sperm quality parameters and the chemical evaluation of the core of the sperm trough Raman Scaterring Spectroscopy, after in vitro exposure to different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) and atrazine. The choice of the use of the these herbicides was because they are widely use throughout the world, especially in Brazil, highlighted the west Paulista region, used at cane sugar plantation, significant culture of the region. In this context, sperm samples were selected according to the normal testing parameters that evaluate sperm quality, such as liquefaction, viscosity, appearance, volume, pH, sperm, vitality, morphology and motility. Semen samples of 10 healthy donor were exposed to study herbicides varying the concentration and time. After the different exposures (0.05, 0.5, 1.0, 5.0 and 125 μg/mL, 30 and 60 min), the analysis of motility and vitality were again evaluated. The Raman Scaterring spectra were obtained from samples control sperm and after exposure to the herbicide 2,4-D e atrazine at concentration of 125 μg/mL and 60 min incubation. The progressive motility and total motility decreased from the concentration 0,5 μg/mL at times 30 and 60 minutes while the percentage of sperm properties increased. The progressive motility and total motility decreased from the concentration 0.5 mg / mL at times 30 and 60 minutes while the percentage of sperm immotile increased. The vitality (viable sperm) decreased significantly (p <0.05) from the exposure concentration 0.5 μg/mL to 2,4-D and from 1.0 μg/mL atrazine, at times 30 and 60 minutes, showing that there is a functional change of trend of human sperm after exposure to these herbicides, and this change is intensified with the exposure time factor and concentration of herbicides. Through the Raman Scaterring technique allowed us to characterize normal human sperm and exposed to herbicides and verify the change in the molecular structure in the exposed sperm, evidenced by changes in relative intensities of the bands 785, 1095, 1255, 1336, 1374, 1486 and 1575 cm-1 treated with 2,4-D and a decrease at 1095 after exposure to atrazine. These results indicate the modification of nitrogenous bases and the phosphate group that make up DNA present in the nucleus of the sperm. |
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Antunes, Patrícia AlexandraCPF:17871635808http://lattes.cnpq.br/6801270542810402Favareto, Ana Paula AlvesCPF:31544492804http://lattes.cnpq.br/2816211314670217Constantino, Carlos José LeopoldoCPF:0674147383http://lattes.cnpq.br/6118325967319836Simões, Rebeca DelatoreCPF:21618359800http://lattes.cnpq.br/1540538724619296CPF:38435401871http://lattes.cnpq.br/7294444614415700Oliveira, Tamiris Garbiatti de2016-07-18T17:46:21Z2015-06-122015-03-23OLIVEIRA, Tamiris Garbiatti de. In vitro Interaction study between herbicides and human sperm using Raman Scaterring Spectroscopy. 2015. 99 f. Dissertação (Mestrado em Ciências Ambientais) - Universidade do Oeste Paulista, Presidente Prudente, 2015.http://bdtd.unoeste.br:8080/tede/handle/tede/493The unlimited use of pesticides, besides contribute with to increase environmental pollution, is also influencing negatively the health of the living beings, including the people health. Many studies show the result these chemicals in the reproductive system of animals and even same at man, can cause infertility problem, which can originate, among other factors, the low sperm quality and quantity. This is study aimed at evaluating human sperm quality parameters and the chemical evaluation of the core of the sperm trough Raman Scaterring Spectroscopy, after in vitro exposure to different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) and atrazine. The choice of the use of the these herbicides was because they are widely use throughout the world, especially in Brazil, highlighted the west Paulista region, used at cane sugar plantation, significant culture of the region. In this context, sperm samples were selected according to the normal testing parameters that evaluate sperm quality, such as liquefaction, viscosity, appearance, volume, pH, sperm, vitality, morphology and motility. Semen samples of 10 healthy donor were exposed to study herbicides varying the concentration and time. After the different exposures (0.05, 0.5, 1.0, 5.0 and 125 μg/mL, 30 and 60 min), the analysis of motility and vitality were again evaluated. The Raman Scaterring spectra were obtained from samples control sperm and after exposure to the herbicide 2,4-D e atrazine at concentration of 125 μg/mL and 60 min incubation. The progressive motility and total motility decreased from the concentration 0,5 μg/mL at times 30 and 60 minutes while the percentage of sperm properties increased. The progressive motility and total motility decreased from the concentration 0.5 mg / mL at times 30 and 60 minutes while the percentage of sperm immotile increased. The vitality (viable sperm) decreased significantly (p <0.05) from the exposure concentration 0.5 μg/mL to 2,4-D and from 1.0 μg/mL atrazine, at times 30 and 60 minutes, showing that there is a functional change of trend of human sperm after exposure to these herbicides, and this change is intensified with the exposure time factor and concentration of herbicides. Through the Raman Scaterring technique allowed us to characterize normal human sperm and exposed to herbicides and verify the change in the molecular structure in the exposed sperm, evidenced by changes in relative intensities of the bands 785, 1095, 1255, 1336, 1374, 1486 and 1575 cm-1 treated with 2,4-D and a decrease at 1095 after exposure to atrazine. These results indicate the modification of nitrogenous bases and the phosphate group that make up DNA present in the nucleus of the sperm.A utilização desenfreada de praguicidas, além de contribuir com o agravamento da poluição ambiental, também está influenciando de forma negativa a saúde dos seres vivos, inclusive a saúde das pessoas. Muitos estudos mostram a interferência desses produtos químicos no sistema reprodutor de animais e até mesmo do homem, podendo acarretar problemas de infertilidade, que podem ser originados, dentre vários fatores, pela baixa quantidade e qualidade espermática. Este estudo visa á avaliação de parâmetros de qualidade espermática humana e a avaliação química do núcleo do espermatozoide, por meio de Espectroscopia de Espalhamento Raman, após a exposição in vitro a diferentes concentrações de ácido 2,4-diclorofenoxiacético (2,4-D) e atrazina. Estes herbicidas são amplamente utilizados em todo o mundo, principalmente no Brasil, em destaque a região do Oeste Paulista, por serem empregados na cana-de-açúcar, cultura significativa da região. Neste contexto, foram selecionadas amostras de espermatozoides de acordo com os parâmetros normais de testes que avaliam a qualidade espermática, como: liquefação, viscosidade, aparência, volume, pH, concentração de espermatozoides, vitalidade, morfologia e motilidade. Amostras de sêmen de 10 doadores saudáveis foram expostas aos herbicidas de estudo, variando o tempo e a concentração. Após as diferentes exposições (0,05; 0,5; 1,0; 5,0 e 125 μg/mL, 30 e 60 min), as análises de motilidade e vitalidade foram novamente avaliadas. Os espectros de Espalhamento Raman foram obtidos das amostras de espermatozoide controle e após a exposição aos herbicidas 2,4-D e atrazina na concentração de 125 µg/mL em 60 min de incubação. A motilidade progressiva e motilidade total diminuíram a partir da concentração 0,5 μg/mL nos tempos 30 e 60 min enquanto que a porcentagem de espermatozoides imóveis aumentou. A vitalidade (espermatozoides viáveis) diminuiu significativamente (p<0,05) a partir da exposição da concentração 0,5 μg/mL para o 2,4-D e a partir de 1,0 μg/mL para a atrazina, nos tempos 30 e 60min, mostrando-se que há uma tendência de alteração funcional do espermatozoide humano após a exposição a estes herbicidas, e esta alteração é intensificada com o fator de tempo de exposição e concentração dos herbicidas testados. Por meio da técnica de Espalhamento Raman foi possível caracterizar o espermatozoide humano normal e os expostos aos herbicidas e verificar a alteração na estrutura molecular nos espermatozoides expostos, evidenciadas pelas alterações nas intensidades relativas das bandas 785, 1095, 1255, 1336, 1374, 1486 e 1575 cm-1 do tratado com 2,4-D, e uma diminuição na banda de 1095 após a exposição à atrazina. Estes resultados indicam a alteração das bases nitrogenadas e do grupo fosfato que compõem o DNA presente no núcleo do espermatozoide.Made available in DSpace on 2016-07-18T17:46:21Z (GMT). No. of bitstreams: 1 Tamiris Garbiatti de Oliveira.pdf: 1606086 bytes, checksum: cf9e26811174e34243865dc9dfffec23 (MD5) Previous issue date: 2015-03-23application/pdfhttp://bdtd.unoeste.br:8080/jspui/retrieve/1666/Tamiris%20Garbiatti%20de%20Oliveira.pdf.jpgporUniversidade do Oeste PaulistaMestrado em Meio Ambiente e Desenvolvimento RegionalUNOESTEBRCiências Ambientais2,4-DAtrazinaEspermatozoide HumanoEspectroscopia de Espalhamento Raman2,4-DAtrazineHuman SpermRaman Scattering spectroscopyCNPQ::Estudo in vitro da interação entre herbicidas e espermatozoide humano utilizando a Espectroscopia de Espalhamento RamanIn vitro Interaction study between herbicides and human sperm using Raman Scaterring Spectroscopyinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UNOESTEinstname:Universidade do Oeste Paulista (UNOESTE)instacron:UNOESTETHUMBNAILTamiris Garbiatti de Oliveira.pdf.jpgTamiris Garbiatti de Oliveira.pdf.jpgimage/jpeg2132http://bdtd.unoeste.br:8080/tede/bitstream/tede/493/2/Tamiris+Garbiatti+de+Oliveira.pdf.jpg92817dabe311ce823163fdb448c534b6MD52ORIGINALTamiris Garbiatti de Oliveira.pdfapplication/pdf1606086http://bdtd.unoeste.br:8080/tede/bitstream/tede/493/1/Tamiris+Garbiatti+de+Oliveira.pdfcf9e26811174e34243865dc9dfffec23MD51tede/4932016-07-19 01:01:38.465oai:bdtd.unoeste.br:tede/493Biblioteca Digital de Teses e Dissertaçõeshttp://bdtd.unoeste.br:8080/jspui/PUBhttp://bdtd.unoeste.br:8080/oai/requestbdtd@unoeste.bropendoar:2016-07-19T04:01:38Biblioteca Digital de Teses e Dissertações da UNOESTE - Universidade do Oeste Paulista (UNOESTE)false |
dc.title.por.fl_str_mv |
Estudo in vitro da interação entre herbicidas e espermatozoide humano utilizando a Espectroscopia de Espalhamento Raman |
dc.title.alternative.eng.fl_str_mv |
In vitro Interaction study between herbicides and human sperm using Raman Scaterring Spectroscopy |
title |
Estudo in vitro da interação entre herbicidas e espermatozoide humano utilizando a Espectroscopia de Espalhamento Raman |
spellingShingle |
Estudo in vitro da interação entre herbicidas e espermatozoide humano utilizando a Espectroscopia de Espalhamento Raman Oliveira, Tamiris Garbiatti de 2,4-D Atrazina Espermatozoide Humano Espectroscopia de Espalhamento Raman 2,4-D Atrazine Human Sperm Raman Scattering spectroscopy CNPQ:: |
title_short |
Estudo in vitro da interação entre herbicidas e espermatozoide humano utilizando a Espectroscopia de Espalhamento Raman |
title_full |
Estudo in vitro da interação entre herbicidas e espermatozoide humano utilizando a Espectroscopia de Espalhamento Raman |
title_fullStr |
Estudo in vitro da interação entre herbicidas e espermatozoide humano utilizando a Espectroscopia de Espalhamento Raman |
title_full_unstemmed |
Estudo in vitro da interação entre herbicidas e espermatozoide humano utilizando a Espectroscopia de Espalhamento Raman |
title_sort |
Estudo in vitro da interação entre herbicidas e espermatozoide humano utilizando a Espectroscopia de Espalhamento Raman |
author |
Oliveira, Tamiris Garbiatti de |
author_facet |
Oliveira, Tamiris Garbiatti de |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Antunes, Patrícia Alexandra |
dc.contributor.advisor1ID.fl_str_mv |
CPF:17871635808 |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/6801270542810402 |
dc.contributor.advisor-co1.fl_str_mv |
Favareto, Ana Paula Alves |
dc.contributor.advisor-co1ID.fl_str_mv |
CPF:31544492804 |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://lattes.cnpq.br/2816211314670217 |
dc.contributor.referee1.fl_str_mv |
Constantino, Carlos José Leopoldo |
dc.contributor.referee1ID.fl_str_mv |
CPF:0674147383 |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/6118325967319836 |
dc.contributor.referee2.fl_str_mv |
Simões, Rebeca Delatore |
dc.contributor.referee2ID.fl_str_mv |
CPF:21618359800 |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/1540538724619296 |
dc.contributor.authorID.fl_str_mv |
CPF:38435401871 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/7294444614415700 |
dc.contributor.author.fl_str_mv |
Oliveira, Tamiris Garbiatti de |
contributor_str_mv |
Antunes, Patrícia Alexandra Favareto, Ana Paula Alves Constantino, Carlos José Leopoldo Simões, Rebeca Delatore |
dc.subject.por.fl_str_mv |
2,4-D Atrazina Espermatozoide Humano Espectroscopia de Espalhamento Raman |
topic |
2,4-D Atrazina Espermatozoide Humano Espectroscopia de Espalhamento Raman 2,4-D Atrazine Human Sperm Raman Scattering spectroscopy CNPQ:: |
dc.subject.eng.fl_str_mv |
2,4-D Atrazine Human Sperm Raman Scattering spectroscopy |
dc.subject.cnpq.fl_str_mv |
CNPQ:: |
description |
The unlimited use of pesticides, besides contribute with to increase environmental pollution, is also influencing negatively the health of the living beings, including the people health. Many studies show the result these chemicals in the reproductive system of animals and even same at man, can cause infertility problem, which can originate, among other factors, the low sperm quality and quantity. This is study aimed at evaluating human sperm quality parameters and the chemical evaluation of the core of the sperm trough Raman Scaterring Spectroscopy, after in vitro exposure to different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) and atrazine. The choice of the use of the these herbicides was because they are widely use throughout the world, especially in Brazil, highlighted the west Paulista region, used at cane sugar plantation, significant culture of the region. In this context, sperm samples were selected according to the normal testing parameters that evaluate sperm quality, such as liquefaction, viscosity, appearance, volume, pH, sperm, vitality, morphology and motility. Semen samples of 10 healthy donor were exposed to study herbicides varying the concentration and time. After the different exposures (0.05, 0.5, 1.0, 5.0 and 125 μg/mL, 30 and 60 min), the analysis of motility and vitality were again evaluated. The Raman Scaterring spectra were obtained from samples control sperm and after exposure to the herbicide 2,4-D e atrazine at concentration of 125 μg/mL and 60 min incubation. The progressive motility and total motility decreased from the concentration 0,5 μg/mL at times 30 and 60 minutes while the percentage of sperm properties increased. The progressive motility and total motility decreased from the concentration 0.5 mg / mL at times 30 and 60 minutes while the percentage of sperm immotile increased. The vitality (viable sperm) decreased significantly (p <0.05) from the exposure concentration 0.5 μg/mL to 2,4-D and from 1.0 μg/mL atrazine, at times 30 and 60 minutes, showing that there is a functional change of trend of human sperm after exposure to these herbicides, and this change is intensified with the exposure time factor and concentration of herbicides. Through the Raman Scaterring technique allowed us to characterize normal human sperm and exposed to herbicides and verify the change in the molecular structure in the exposed sperm, evidenced by changes in relative intensities of the bands 785, 1095, 1255, 1336, 1374, 1486 and 1575 cm-1 treated with 2,4-D and a decrease at 1095 after exposure to atrazine. These results indicate the modification of nitrogenous bases and the phosphate group that make up DNA present in the nucleus of the sperm. |
publishDate |
2015 |
dc.date.available.fl_str_mv |
2015-06-12 |
dc.date.issued.fl_str_mv |
2015-03-23 |
dc.date.accessioned.fl_str_mv |
2016-07-18T17:46:21Z |
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info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
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publishedVersion |
dc.identifier.citation.fl_str_mv |
OLIVEIRA, Tamiris Garbiatti de. In vitro Interaction study between herbicides and human sperm using Raman Scaterring Spectroscopy. 2015. 99 f. Dissertação (Mestrado em Ciências Ambientais) - Universidade do Oeste Paulista, Presidente Prudente, 2015. |
dc.identifier.uri.fl_str_mv |
http://bdtd.unoeste.br:8080/tede/handle/tede/493 |
identifier_str_mv |
OLIVEIRA, Tamiris Garbiatti de. In vitro Interaction study between herbicides and human sperm using Raman Scaterring Spectroscopy. 2015. 99 f. Dissertação (Mestrado em Ciências Ambientais) - Universidade do Oeste Paulista, Presidente Prudente, 2015. |
url |
http://bdtd.unoeste.br:8080/tede/handle/tede/493 |
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por |
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por |
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info:eu-repo/semantics/openAccess |
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Universidade do Oeste Paulista |
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Mestrado em Meio Ambiente e Desenvolvimento Regional |
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UNOESTE |
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BR |
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Ciências Ambientais |
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Universidade do Oeste Paulista |
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