Estudo das vias de descarboxilação da fotossíntese C4 em cana-de-açúcar submetida ao déficit hídrico
Autor(a) principal: | |
---|---|
Data de Publicação: | 2017 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da UNOESTE |
Texto Completo: | http://bdtd.unoeste.br:8080/jspui/handle/jspui/1014 |
Resumo: | C4 plants have been classified into three groups based on the enzymes used to decarboxylate C4 acids in the bundle sheath: NADP-ME, NAD-ME and PEPCK subtypes. Numerous molecular, biochemical and physiological evidences indicate that C4 plants could exhibit a certain degree of flexibility in the use of the three established decarboxylation mechanisms, depending on environmental factors. In this context, the objective of this work was to evaluate the modulation of the pathways of decarboxylation of the C4 photosynthesis in sugarcane (NADP-ME specie) under water deficit. The experiment was realized with two genotypes - RB92579 (tolerant to water deficit) and SP80-3280 (susceptible to water deficit) submitted to four treatments: control (normal conditions of water supply), moderate stress (-1,5 to -1,8 MPa), severe stress (below -2,0 MPa) and recovery (48 hours after rehydration). Leaf water potential, leaf gas exchange and biomass production were measured for the physiological characterization of the plants. Changes in the transcriptional responses of genes encoding C4-cycle enzymes (NADP-ME, NAD-ME, PEPCK, AspAT, AlaAT, PEPC, NADP-MDH and PPDK) and the activities of the enzymes NADP-ME, NAD-ME, PEPCK, AspAT and AlaAT were analyzed by RT-qPCR and spectrophotometry, respectively. Under water deficit conditions the genotypes SP80-3280 was more sensitive to drought stress in terms of increased loss of above ground biomass and lower leaf water potential. It also showed less capacity of photosynthetic recovery following rehydration compared to the tolerant genotypes RB92579. The analysis of transcriptionals and of the activities of enzymes involved in the C4 photosynthetic pathway showed that sugarcane uses the PEPCK pathway as a decarboxylation mechanism in addition to the NADP-ME, which was more evident under water deficit conditions for both the drought tolerant and susceptible genotypes. Finally, the results here obtained, together with the existing information, do not support the established classification of sugarcane (Saccharum spp.) as a classical NADP-ME C4 subtype, but instead should be considered as a NADP-ME + PEPCK species. |
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Vieira, Luiz Gonzaga Esteves232.163.959-87http://lattes.cnpq.br/2988164955239991Ribas, Alessandra Ferreira021.521.619-98http://lattes.cnpq.br/9273720281917978Domingues, Douglas Silva297.659.208-06http://lattes.cnpq.br/7905667701769534Santos, Tiago Benedito dos296.718.608-26http://lattes.cnpq.br/526088707521922413.906.788-81http://lattes.cnpq.br/8585120655579750Cacefo, Viviane2017-06-13T13:30:56Z2017-02-17CACEFO, Viviane. Estudo das vias de descarboxilação da fotossíntese C4 em cana-de-açúcar submetida ao déficit hídrico. 2017. 83f. Dissertação (Mestrado em Agronomia) - Universidade do Oeste Paulista, Presidente Prudente, 2017.http://bdtd.unoeste.br:8080/jspui/handle/jspui/1014C4 plants have been classified into three groups based on the enzymes used to decarboxylate C4 acids in the bundle sheath: NADP-ME, NAD-ME and PEPCK subtypes. Numerous molecular, biochemical and physiological evidences indicate that C4 plants could exhibit a certain degree of flexibility in the use of the three established decarboxylation mechanisms, depending on environmental factors. In this context, the objective of this work was to evaluate the modulation of the pathways of decarboxylation of the C4 photosynthesis in sugarcane (NADP-ME specie) under water deficit. The experiment was realized with two genotypes - RB92579 (tolerant to water deficit) and SP80-3280 (susceptible to water deficit) submitted to four treatments: control (normal conditions of water supply), moderate stress (-1,5 to -1,8 MPa), severe stress (below -2,0 MPa) and recovery (48 hours after rehydration). Leaf water potential, leaf gas exchange and biomass production were measured for the physiological characterization of the plants. Changes in the transcriptional responses of genes encoding C4-cycle enzymes (NADP-ME, NAD-ME, PEPCK, AspAT, AlaAT, PEPC, NADP-MDH and PPDK) and the activities of the enzymes NADP-ME, NAD-ME, PEPCK, AspAT and AlaAT were analyzed by RT-qPCR and spectrophotometry, respectively. Under water deficit conditions the genotypes SP80-3280 was more sensitive to drought stress in terms of increased loss of above ground biomass and lower leaf water potential. It also showed less capacity of photosynthetic recovery following rehydration compared to the tolerant genotypes RB92579. The analysis of transcriptionals and of the activities of enzymes involved in the C4 photosynthetic pathway showed that sugarcane uses the PEPCK pathway as a decarboxylation mechanism in addition to the NADP-ME, which was more evident under water deficit conditions for both the drought tolerant and susceptible genotypes. Finally, the results here obtained, together with the existing information, do not support the established classification of sugarcane (Saccharum spp.) as a classical NADP-ME C4 subtype, but instead should be considered as a NADP-ME + PEPCK species.As plantas C4 são classificadas em três grupos de acordo com as enzimas utilizadas para descarboxilação de ácidos C4 na bainha dos feixes vasculares: subtipos NADP-ME, NAD-ME e PEPCK. Inúmeras evidências moleculares, bioquímicas e fisiológicas indicam que plantas C4 podem exibir certo nível de flexibilidade na utilização dos três mecanismos de descarboxilação, dependendo de fatores ambientais. Neste contexto, o objetivo deste trabalho foi avaliar a modulação das vias de descarboxilação da fotossíntese C4 em cana-de-açúcar (espécie NADP-ME) sob déficit hídrico. O experimento foi realizado com dois genótipos - RB92579 (tolerante ao déficit hídrico) e SP80-3280 (suscetível ao déficit hídrico) submetidos a quatro tratamentos: controle (condições normais de suprimento de água), estresse moderado (-1,5 a -1,8 MPa), estresse severo (abaixo de -2,0 MPa) e recuperação (48 hrs após a reidratação). Foram realizadas análises de potencial de água foliar, trocas gasosas foliares e biomassa para caracterização fisiológica das plantas. As alterações nas respostas transcricionais dos genes codificadores de enzimas do ciclo C4 (NADP-ME, NAD-ME, PEPCK, AspAT, AlaAT, PEPC, NADP-MDH e PPDK) e atividades das enzimas NADP-ME, NAD-ME, PEPCK, AspAT e AlaAT, foram analisadas por RT-qPCR e espectrofotometria, respectivamente. Em condições de déficit hídrico, o genótipo SP80-3280 foi mais sensível ao estresse por seca em termos de aumento da perda de biomassa e menor potencial de água foliar. Também mostrou menor capacidade de recuperação fotossintética após reidratação do que o genótipo tolerante RB92579. As análises transcricionais e das atividades das enzimas envolvidas na via fotossintética C4 mostraram que a cana-de-açúcar utiliza a via PEPCK como mecanismo de descarboxilação, além da NADP-ME, mais evidente em condições de déficit hídrico tanto para genótipos tolerantes à seca como para suscetíveis. Finalmente, os resultados aqui obtidos, juntamente com a informação existente, não suportam a classificação estabelecida da cana-de-açúcar (Saccharum spp.) como um subtipo C4 NADP-ME clássico, mas considerá-la como uma espécie NADP-ME + PEPCK.Submitted by Michele Mologni (mologni@unoeste.br) on 2017-06-13T13:30:56Z No. of bitstreams: 1 Viviane Cacefo.pdf: 1556310 bytes, checksum: 3f9d8dc2ff4a08b8acc2ec7e1a731117 (MD5)Made available in DSpace on 2017-06-13T13:30:56Z (GMT). No. of bitstreams: 1 Viviane Cacefo.pdf: 1556310 bytes, checksum: 3f9d8dc2ff4a08b8acc2ec7e1a731117 (MD5) Previous issue date: 2017-02-17application/pdfhttp://bdtd.unoeste.br:8080/jspui/retrieve/2253/Viviane%20Cacefo.pdf.jpgporUniversidade do Oeste PaulistaMestrado em AgronomiaUNOESTEBrasilMestrado em AgronomiaSaccharum spp. Estresse Abiótico. Regulação Gênica. Atividade Enzimática.Saccharum spp. Abiotic Stress. Gene Regulation. Enzymatic Activity.CIENCIAS AGRARIAS::AGRONOMIAEstudo das vias de descarboxilação da fotossíntese C4 em cana-de-açúcar submetida ao déficit hídricoStudy of the decarboxylation pathways of C4 photosynthesis in sugarcane submitted to water deficitinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis308868666724573970500500600308868666724573970-3091138714907603907info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UNOESTEinstname:Universidade do Oeste Paulista (UNOESTE)instacron:UNOESTELICENSElicense.txtlicense.txttext/plain; charset=utf-82067http://bdtd.unoeste.br:8080/tede/bitstream/jspui/1014/1/license.txt47745281809acb27fb322a97f2d9cb88MD51ORIGINALViviane Cacefo.pdfViviane Cacefo.pdfapplication/pdf1556310http://bdtd.unoeste.br:8080/tede/bitstream/jspui/1014/2/Viviane+Cacefo.pdf3f9d8dc2ff4a08b8acc2ec7e1a731117MD52THUMBNAILViviane Cacefo.pdf.jpgViviane Cacefo.pdf.jpgimage/jpeg2244http://bdtd.unoeste.br:8080/tede/bitstream/jspui/1014/3/Viviane+Cacefo.pdf.jpgbef6683c6aa60916f87893431e161177MD53jspui/10142017-06-14 01:00:08.074oai:bdtd.unoeste.br:jspui/1014TElDRU7Dh0EgREUgRElTVFJJQlVJw4fDg08gTsODTyBFWENMVVNJVkEKCkNvbSBhIGFwcmVzZW50YcOnw6NvIGRlc3RhIGxpY2Vuw6dhLCB2b2PDqiAobm9tZSBkbyBhdXRvcikgY29uY2VkZSDDoCBVbml2ZXJzaWRhZGUgZG8gT2VzdGUgUGF1bGlzdGEg4oCTIFVOT0VTVEUsIG8gZGlyZWl0byBuw6NvLWV4Y2x1c2l2byBkZSByZXByb2R1emlyLCAgdHJhZHV6aXIgKGNvbmZvcm1lIGRlZmluaWRvIGFiYWl4byksIGUvb3UgZGlzdHJpYnVpciBhIHN1YSB0ZXNlIG91IGRpc3NlcnRhw6fDo28gKGluY2x1aW5kbyBvIHJlc3VtbykgcG9yIHRvZG8gbyBtdW5kbyBubyBmb3JtYXRvIGltcHJlc3NvIGUgZWxldHLDtG5pY28gZSBlbSBxdWFscXVlciBtZWlvLCBpbmNsdWluZG8gb3MgZm9ybWF0b3Mgw6F1ZGlvIG91IHbDrWRlby4KClZvY8OqIGNvbmNvcmRhIHF1ZSBhIFVOT0VTVEUgcG9kZSwgc2VtIGFsdGVyYXIgbyBjb250ZcO6ZG8sIHRyYW5zcG9yIGEgc3VhIHRlc2Ugb3UgZGlzc2VydGHDp8OjbyBwYXJhIHF1YWxxdWVyIG1laW8gb3UgZm9ybWF0byBwYXJhIGZpbnMgZGUgcHJlc2VydmHDp8Ojby4KClZvY8OqIHRhbWLDqW0gY29uY29yZGEgcXVlIGEgVU5PRVNURSBwb2RlIG1hbnRlciBtYWlzIGRlIHVtYSBjw7NwaWEgZGUgc3VhIHRlc2Ugb3UgZGlzc2VydGHDp8OjbyBwYXJhIGZpbnMgZGUgc2VndXJhbsOnYSwgYmFjay11cCBlIHByZXNlcnZhw6fDo28uCgpWb2PDqiBkZWNsYXJhIHF1ZSBhIHN1YSB0ZXNlIG91IGRpc3NlcnRhw6fDo28gw6kgb3JpZ2luYWwsIGUgcXVlIHZvY8OqIHRlbSBvIHBvZGVyIGRlIGNvbmNlZGVyIG9zIGRpcmVpdG9zIGNvbnRpZG9zIG5lc3RhIGxpY2Vuw6dhLiBWb2PDqiB0YW1iw6ltIGRlY2xhcmEgcXVlIG8gZGVww7NzaXRvIGRhIHN1YSB0ZXNlIG91IGRpc3NlcnRhw6fDo28gbsOjbyBpbmZyaW5nZSwgcXVlIHNlamEgZGUgc2V1IGNvbmhlY2ltZW50bywgZGlyZWl0b3MgYXV0b3JhaXMgZGUgbmluZ3XDqW0uCgpDYXNvIGEgc3VhIHRlc2Ugb3UgZGlzc2VydGHDp8OjbyBjb250ZW5oYSBtYXRlcmlhbCBxdWUgdm9jw6ogbsOjbyBwb3NzdWkgYSB0aXR1bGFyaWRhZGUgZG9zIGRpcmVpdG9zIGF1dG9yYWlzLCB2b2PDqiBkZWNsYXJhIHF1ZSBvYnRldmUgYSBwZXJtaXNzw6NvIGlycmVzdHJpdGEgZG8gZGV0ZW50b3IgZG9zIGRpcmVpdG9zIGF1dG9yYWlzIHBhcmEgY29uY2VkZXIgw6AgVU5PRVNURSBvcyBkaXJlaXRvcyBhcHJlc2VudGFkb3MgbmVzdGEgbGljZW7Dp2EsIGUgcXVlIGVzc2UgbWF0ZXJpYWwgZGUgcHJvcHJpZWRhZGUgZGUgdGVyY2Vpcm9zIGVzdMOhIGNsYXJhbWVudGUgaWRlbnRpZmljYWRvIGUgcmVjb25oZWNpZG8gbm8gdGV4dG8gb3Ugbm8gY29udGXDumRvIGRhIHRlc2Ugb3UgZGlzc2VydGHDp8OjbyBvcmEgZGVwb3NpdGFkYS4KCkNhc28gYSB0ZXNlIG91IGRpc3NlcnRhw6fDo28gb3JhIGRlcG9zaXRhZGEgdGVuaGEgc2lkbyByZXN1bHRhZG8gZGUgdW0gcGF0cm9jw61uaW8gb3UgYXBvaW8gZGUgdW1hIGFnw6puY2lhIGRlIGZvbWVudG8gb3Ugb3V0cm8gb3JnYW5pc21vLCBxdWUgbsOjbyBzZWphIGEgVU5PRVNURSwgdm9jw6ogZGVjbGFyYSBxdWUgcmVzcGVpdG91IHRvZG9zIGUgcXVhaXNxdWVyIGRpcmVpdG9zIGRlIHJldmlzw6NvLCBjb21vIHRhbWLDqW0sIGFzIGRlbWFpcyBvYnJpZ2HDp8O1ZXMgZXhpZ2lkYXMgcG9yIGNvbnRyYXRvIG91IGFjb3Jkby4KCkEgVU5PRVNURSBzZSBjb21wcm9tZXRlIGEgaWRlbnRpZmljYXIgY2xhcmFtZW50ZSBvIHNldSBub21lIChzKSBvdSBvKHMpIG5vbWUocykgZG8ocykgZGV0ZW50b3IgKGVzKSBkb3MgZGlyZWl0b3MgYXV0b3JhaXMgZGEgdGVzZSBvdSBkaXNzZXJ0YcOnw6NvLCBlIG7Do28gZmFyw6EgcXVhbHF1ZXIgYWx0ZXJhw6fDo28sIGFsw6ltIGRhcXVlbGFzIGNvbmNlZGlkYXMgcG9yIGVzdGEgbGljZW7Dp2EuCgpFIHBvciBzZXJlbSB2ZXJkYWRlaXJhcyBhcyBpbmZvcm1hw6fDtWVzIGFjaW1hIGNpdGFkYXMsIGNvbmNlZG8gYSBsaWNlbsOnYSBkZSBkaXN0cmlidWnDp8OjbyBuw6NvIGV4Y2x1c2l2YSBwYXJhIGEgVU5PRVNURS4KBiblioteca Digital de Teses e Dissertaçõeshttp://bdtd.unoeste.br:8080/jspui/PUBhttp://bdtd.unoeste.br:8080/oai/requestbdtd@unoeste.bropendoar:2017-06-14T04:00:08Biblioteca Digital de Teses e Dissertações da UNOESTE - Universidade do Oeste Paulista (UNOESTE)false |
dc.title.por.fl_str_mv |
Estudo das vias de descarboxilação da fotossíntese C4 em cana-de-açúcar submetida ao déficit hídrico |
dc.title.alternative.eng.fl_str_mv |
Study of the decarboxylation pathways of C4 photosynthesis in sugarcane submitted to water deficit |
title |
Estudo das vias de descarboxilação da fotossíntese C4 em cana-de-açúcar submetida ao déficit hídrico |
spellingShingle |
Estudo das vias de descarboxilação da fotossíntese C4 em cana-de-açúcar submetida ao déficit hídrico Cacefo, Viviane Saccharum spp. Estresse Abiótico. Regulação Gênica. Atividade Enzimática. Saccharum spp. Abiotic Stress. Gene Regulation. Enzymatic Activity. CIENCIAS AGRARIAS::AGRONOMIA |
title_short |
Estudo das vias de descarboxilação da fotossíntese C4 em cana-de-açúcar submetida ao déficit hídrico |
title_full |
Estudo das vias de descarboxilação da fotossíntese C4 em cana-de-açúcar submetida ao déficit hídrico |
title_fullStr |
Estudo das vias de descarboxilação da fotossíntese C4 em cana-de-açúcar submetida ao déficit hídrico |
title_full_unstemmed |
Estudo das vias de descarboxilação da fotossíntese C4 em cana-de-açúcar submetida ao déficit hídrico |
title_sort |
Estudo das vias de descarboxilação da fotossíntese C4 em cana-de-açúcar submetida ao déficit hídrico |
author |
Cacefo, Viviane |
author_facet |
Cacefo, Viviane |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Vieira, Luiz Gonzaga Esteves |
dc.contributor.advisor1ID.fl_str_mv |
232.163.959-87 |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/2988164955239991 |
dc.contributor.advisor-co1.fl_str_mv |
Ribas, Alessandra Ferreira |
dc.contributor.advisor-co1ID.fl_str_mv |
021.521.619-98 |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://lattes.cnpq.br/9273720281917978 |
dc.contributor.referee1.fl_str_mv |
Domingues, Douglas Silva |
dc.contributor.referee1ID.fl_str_mv |
297.659.208-06 |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/7905667701769534 |
dc.contributor.referee2.fl_str_mv |
Santos, Tiago Benedito dos |
dc.contributor.referee2ID.fl_str_mv |
296.718.608-26 |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/526088707521922 |
dc.contributor.authorID.fl_str_mv |
413.906.788-81 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/8585120655579750 |
dc.contributor.author.fl_str_mv |
Cacefo, Viviane |
contributor_str_mv |
Vieira, Luiz Gonzaga Esteves Ribas, Alessandra Ferreira Domingues, Douglas Silva Santos, Tiago Benedito dos |
dc.subject.por.fl_str_mv |
Saccharum spp. Estresse Abiótico. Regulação Gênica. Atividade Enzimática. |
topic |
Saccharum spp. Estresse Abiótico. Regulação Gênica. Atividade Enzimática. Saccharum spp. Abiotic Stress. Gene Regulation. Enzymatic Activity. CIENCIAS AGRARIAS::AGRONOMIA |
dc.subject.eng.fl_str_mv |
Saccharum spp. Abiotic Stress. Gene Regulation. Enzymatic Activity. |
dc.subject.cnpq.fl_str_mv |
CIENCIAS AGRARIAS::AGRONOMIA |
description |
C4 plants have been classified into three groups based on the enzymes used to decarboxylate C4 acids in the bundle sheath: NADP-ME, NAD-ME and PEPCK subtypes. Numerous molecular, biochemical and physiological evidences indicate that C4 plants could exhibit a certain degree of flexibility in the use of the three established decarboxylation mechanisms, depending on environmental factors. In this context, the objective of this work was to evaluate the modulation of the pathways of decarboxylation of the C4 photosynthesis in sugarcane (NADP-ME specie) under water deficit. The experiment was realized with two genotypes - RB92579 (tolerant to water deficit) and SP80-3280 (susceptible to water deficit) submitted to four treatments: control (normal conditions of water supply), moderate stress (-1,5 to -1,8 MPa), severe stress (below -2,0 MPa) and recovery (48 hours after rehydration). Leaf water potential, leaf gas exchange and biomass production were measured for the physiological characterization of the plants. Changes in the transcriptional responses of genes encoding C4-cycle enzymes (NADP-ME, NAD-ME, PEPCK, AspAT, AlaAT, PEPC, NADP-MDH and PPDK) and the activities of the enzymes NADP-ME, NAD-ME, PEPCK, AspAT and AlaAT were analyzed by RT-qPCR and spectrophotometry, respectively. Under water deficit conditions the genotypes SP80-3280 was more sensitive to drought stress in terms of increased loss of above ground biomass and lower leaf water potential. It also showed less capacity of photosynthetic recovery following rehydration compared to the tolerant genotypes RB92579. The analysis of transcriptionals and of the activities of enzymes involved in the C4 photosynthetic pathway showed that sugarcane uses the PEPCK pathway as a decarboxylation mechanism in addition to the NADP-ME, which was more evident under water deficit conditions for both the drought tolerant and susceptible genotypes. Finally, the results here obtained, together with the existing information, do not support the established classification of sugarcane (Saccharum spp.) as a classical NADP-ME C4 subtype, but instead should be considered as a NADP-ME + PEPCK species. |
publishDate |
2017 |
dc.date.accessioned.fl_str_mv |
2017-06-13T13:30:56Z |
dc.date.issued.fl_str_mv |
2017-02-17 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
CACEFO, Viviane. Estudo das vias de descarboxilação da fotossíntese C4 em cana-de-açúcar submetida ao déficit hídrico. 2017. 83f. Dissertação (Mestrado em Agronomia) - Universidade do Oeste Paulista, Presidente Prudente, 2017. |
dc.identifier.uri.fl_str_mv |
http://bdtd.unoeste.br:8080/jspui/handle/jspui/1014 |
identifier_str_mv |
CACEFO, Viviane. Estudo das vias de descarboxilação da fotossíntese C4 em cana-de-açúcar submetida ao déficit hídrico. 2017. 83f. Dissertação (Mestrado em Agronomia) - Universidade do Oeste Paulista, Presidente Prudente, 2017. |
url |
http://bdtd.unoeste.br:8080/jspui/handle/jspui/1014 |
dc.language.iso.fl_str_mv |
por |
language |
por |
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308868666724573970 |
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500 500 600 |
dc.relation.department.fl_str_mv |
308868666724573970 |
dc.relation.cnpq.fl_str_mv |
-3091138714907603907 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
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application/pdf |
dc.publisher.none.fl_str_mv |
Universidade do Oeste Paulista |
dc.publisher.program.fl_str_mv |
Mestrado em Agronomia |
dc.publisher.initials.fl_str_mv |
UNOESTE |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Mestrado em Agronomia |
publisher.none.fl_str_mv |
Universidade do Oeste Paulista |
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