AVALIAÇÃO DA VIABILIDADE DE AMOSTRAS BACTERIANAS EM SUPERFÍCIES ABIÓTICAS COM A INFLUÊNCIA DE FLUÍDOS BIOLÓGICOS
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da UNOESTE |
Texto Completo: | http://bdtd.unoeste.br:8080/tede/handle/tede/490 |
Resumo: | The environmental and hospital infections, caused by bacteria which are resistant to a wide variety of antibiotics, have shown increasing records in the last years, manifesting themselves in high mortality and lethality. Recent researches report that the bacteria presents a survival profile on dry surfaces in order to maintain their virulence when exposed to biological fluids such as urine, saliva and blood. The objective of this study was to document - through laboratorial analysis the survival capacity of the main bacteria of medical interest on abiotic surfaces. The adopted procedures were completely conducted in the microbiology laboratory at Unoeste, in Presidente Prudente SP. Standard ATCC strains of Staphylococcus aureus (ATCC25923), Enterococcus faecalis (ATCC29212), Escherichia coli (ATCC35218), Klebsiella pneumoniae (ATCC700603) and Pseudomonas aeruginosa (ATCC27583) were used, to which biological fluids and water were added. The surfaces were tile, synthetic fabric, mattress and cotton fabric. The surfaces were contaminated with suspension composed by the ATCC strains, biological fluids and water in addition to the control containing only the ATCC strains, which were stored in petri dishes, kept in room temperature. Every seven days the surfaces were dipped in trypticase soy broth and kept in the oven for 24h at 37ºC. They were spread from the contaminated brothin Mueller Hinton agar and kept for 24h in the oven at 37ºC. The viability analysis was done through the colony-forming unity (CFU) counting. For the statistical analysis, the Software R was used, through the Friedman and Kruskal Wallis tests for multiple comparisons, analyzing within each factor (solution or surface) which differ between one another (Friedman s test) and also which treatments interactions are different (Kruskal-Wallis). Through this analysis it was possible to observe that in the current work that Staphylococcus aureus kept its viability for a longer period than other microorganisms under all the tested conditions, presenting significant differences between the fluids and surfaces, with a particular colony growth in blood and cotton. The Klebsiella pneumoniae showed differences between fluids, with saliva containing the highest colony number. With regards to the other tested bacteria, there was no isolated significance. For the multiple comparisons analysis, only the Pseudomonas aeruginosa presented no significant difference between any pair of treatments, whereas the other bacteria presented significant differences between the correlations. The need to analyze the environmental impact of these microorganisms persistence in environments, which are vulnerable to human beings, guides the creation of measures in order to control the spread of pathogenic microorganisms. |
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Rodrigues, Marcus Vinícius PimentaCPF:27602785819http://lattes.cnpq.br/0133739307461180Favareto, Ana Paula AlvesCPF:31544492804http://lattes.cnpq.br/2816211314670217Martins, AndréCPF:29647935811http://lattes.cnpq.br/9735485654252852CPF:29039615810http://lattes.cnpq.br/8624795479325388Esteves, Deigilam Cestari2016-07-18T17:46:20Z2015-06-102014-12-18ESTEVES, Deigilam Cestari. BACTERIAL SAMPLES OF FEASIBILITY ASSESSMENT SURFACE ABIOTIC WITH INFLUENCE BIOLOGICAL FLUIDS. 2014. 82 f. Dissertação (Mestrado em Ciências Ambientais) - Universidade do Oeste Paulista, Presidente Prudente, 2014.http://bdtd.unoeste.br:8080/tede/handle/tede/490The environmental and hospital infections, caused by bacteria which are resistant to a wide variety of antibiotics, have shown increasing records in the last years, manifesting themselves in high mortality and lethality. Recent researches report that the bacteria presents a survival profile on dry surfaces in order to maintain their virulence when exposed to biological fluids such as urine, saliva and blood. The objective of this study was to document - through laboratorial analysis the survival capacity of the main bacteria of medical interest on abiotic surfaces. The adopted procedures were completely conducted in the microbiology laboratory at Unoeste, in Presidente Prudente SP. Standard ATCC strains of Staphylococcus aureus (ATCC25923), Enterococcus faecalis (ATCC29212), Escherichia coli (ATCC35218), Klebsiella pneumoniae (ATCC700603) and Pseudomonas aeruginosa (ATCC27583) were used, to which biological fluids and water were added. The surfaces were tile, synthetic fabric, mattress and cotton fabric. The surfaces were contaminated with suspension composed by the ATCC strains, biological fluids and water in addition to the control containing only the ATCC strains, which were stored in petri dishes, kept in room temperature. Every seven days the surfaces were dipped in trypticase soy broth and kept in the oven for 24h at 37ºC. They were spread from the contaminated brothin Mueller Hinton agar and kept for 24h in the oven at 37ºC. The viability analysis was done through the colony-forming unity (CFU) counting. For the statistical analysis, the Software R was used, through the Friedman and Kruskal Wallis tests for multiple comparisons, analyzing within each factor (solution or surface) which differ between one another (Friedman s test) and also which treatments interactions are different (Kruskal-Wallis). Through this analysis it was possible to observe that in the current work that Staphylococcus aureus kept its viability for a longer period than other microorganisms under all the tested conditions, presenting significant differences between the fluids and surfaces, with a particular colony growth in blood and cotton. The Klebsiella pneumoniae showed differences between fluids, with saliva containing the highest colony number. With regards to the other tested bacteria, there was no isolated significance. For the multiple comparisons analysis, only the Pseudomonas aeruginosa presented no significant difference between any pair of treatments, whereas the other bacteria presented significant differences between the correlations. The need to analyze the environmental impact of these microorganisms persistence in environments, which are vulnerable to human beings, guides the creation of measures in order to control the spread of pathogenic microorganisms.As infecções ambientais e hospitalares causadas por bactérias resistentes a um amplo espectro de antibióticos têm índices crescentes nos últimos anos, manifestando-se com alta morbidade e letalidade. Pesquisas recentes evidenciam que as bactérias demonstram um perfil de sobrevivência, em superfícies secas de modo a manter sua virulência quando expostas a fluidos biológicos como urina, saliva e sangue. O objetivo desse estudo foi documentar através de análises laboratoriais a capacidade de sobrevivência das principais bactérias de interesse médico em superfícies abióticas. Os procedimentos foram totalmente realizados no laboratório de microbiologia da Unoeste em Presidente Prudente - SP., utilizando cepas padrão ATCC de Staphylococcus aureus(ATCC25923), Enterococcus faecalis (ATCC29212), Escherichia coli(ATCC35218), Klebsiella pneumoniae(ATCC700603) e Pseudomonas aeruginosa(ATCC27583), ao qual foram adicionados fluídos biológicos e água. As superfícies utilizadas foram piso, tecido sintético, colchão e tecido algodão. As superfícies foram contaminadas com suspensão composta pelas cepas ATCC, fluídos biológicos e água, além do controle contendo somente as cepas ATCC, armazenadas em placas de petri e mantidas em temperatura ambiente. A cada sete dias as superfíciesarmazenadas foram mergulhadas em caldo Tripscaseína de Soja (TSB), colocadas na estufa por 24h a 37ºC. Foram semeados do caldo contaminado em ágar Mueller Hinton e mantidos por 24h em aquecimento a 37º em Estufa.A análise da viabilidadefoi realizada através da contagem de unidade formadora de colônias (U.F.C.). Para a análise estatística utilizou o software R, realizando os testes de Friedman e de Kruskal Wallis para comparações multiplas, analisando dentro de cada fator (solução ou superfície) quais diferem entre si (Teste de Friedman) e, também, quais interações de tratamentos são diferentes (Kruskal-Wallis). Através dessas análises foi possível observar no presente trabalho que Staphylococcus aureus manteve a viabilidade por tempo maior que os outros microrganismos em todas as condições testadas, apresentando diferença significativa entre os fluídos e as superfícies, tendo sangue e tecido algodão crescimento de maior número de colônias. A Klebsiella pneumoniae apresentou diferença entre os fluídos, sendo asaliva com maior número de colônias. Para as outras bactérias testadas não houve significância isoladamente. Para as análises de comparações múltiplas somente para a Pseudomonas aeruginosanão houve diferença significativa entre algum par de tratamentos, as outras bactérias apresentaram diferenças significativas entre as correlações. A necessidade de analisar o impacto ambiental da persistência desses microrganismos em ambientes vulneráveis ao ser humano, norteia o delineamento de medidas para o controle na disseminação de microrganismos patogênicos.Made available in DSpace on 2016-07-18T17:46:20Z (GMT). No. of bitstreams: 1 Deigilam Cestari Esteves.pdf: 2776358 bytes, checksum: 7e1a46b5b264cbf8e0c0d96e68b501e2 (MD5) Previous issue date: 2014-12-18application/pdfhttp://bdtd.unoeste.br:8080/jspui/retrieve/1677/Deigilam%20Cestari%20Esteves.pdf.jpgporUniversidade do Oeste PaulistaMestrado em Meio Ambiente e Desenvolvimento RegionalUNOESTEBRCiências Ambientaisviabilidade bacterianasuperfícies abióticasfluídos biológicoscontaminação ambientalBacterial viabilityabiotic surfacesbiological fluidsenvironmental contaminationCNPQ::AVALIAÇÃO DA VIABILIDADE DE AMOSTRAS BACTERIANAS EM SUPERFÍCIES ABIÓTICAS COM A INFLUÊNCIA DE FLUÍDOS BIOLÓGICOSAVALIAÇÃO DA VIABILIDADE DE AMOSTRAS BACTERIANAS EM SUPERFÍCIES ABIÓTICAS COM A INFLUÊNCIA DE FLUÍDOS BIOLÓGICOSBACTERIAL SAMPLES OF FEASIBILITY ASSESSMENT SURFACE ABIOTIC WITH INFLUENCE BIOLOGICAL FLUIDSBACTERIAL SAMPLES OF FEASIBILITY ASSESSMENT SURFACE ABIOTIC WITH INFLUENCE BIOLOGICAL FLUIDSinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UNOESTEinstname:Universidade do Oeste Paulista (UNOESTE)instacron:UNOESTETHUMBNAILDeigilam Cestari Esteves.pdf.jpgDeigilam Cestari Esteves.pdf.jpgimage/jpeg2155http://bdtd.unoeste.br:8080/tede/bitstream/tede/490/2/Deigilam+Cestari+Esteves.pdf.jpgd664def7ea01a565bb13ff9a26ca8fb6MD52ORIGINALDeigilam Cestari Esteves.pdfapplication/pdf2776358http://bdtd.unoeste.br:8080/tede/bitstream/tede/490/1/Deigilam+Cestari+Esteves.pdf7e1a46b5b264cbf8e0c0d96e68b501e2MD51tede/4902016-07-19 01:01:40.799oai:bdtd.unoeste.br:tede/490Biblioteca Digital de Teses e Dissertaçõeshttp://bdtd.unoeste.br:8080/jspui/PUBhttp://bdtd.unoeste.br:8080/oai/requestbdtd@unoeste.bropendoar:2016-07-19T04:01:40Biblioteca Digital de Teses e Dissertações da UNOESTE - Universidade do Oeste Paulista (UNOESTE)false |
dc.title.por.fl_str_mv |
AVALIAÇÃO DA VIABILIDADE DE AMOSTRAS BACTERIANAS EM SUPERFÍCIES ABIÓTICAS COM A INFLUÊNCIA DE FLUÍDOS BIOLÓGICOS AVALIAÇÃO DA VIABILIDADE DE AMOSTRAS BACTERIANAS EM SUPERFÍCIES ABIÓTICAS COM A INFLUÊNCIA DE FLUÍDOS BIOLÓGICOS |
dc.title.alternative.eng.fl_str_mv |
BACTERIAL SAMPLES OF FEASIBILITY ASSESSMENT SURFACE ABIOTIC WITH INFLUENCE BIOLOGICAL FLUIDS BACTERIAL SAMPLES OF FEASIBILITY ASSESSMENT SURFACE ABIOTIC WITH INFLUENCE BIOLOGICAL FLUIDS |
title |
AVALIAÇÃO DA VIABILIDADE DE AMOSTRAS BACTERIANAS EM SUPERFÍCIES ABIÓTICAS COM A INFLUÊNCIA DE FLUÍDOS BIOLÓGICOS |
spellingShingle |
AVALIAÇÃO DA VIABILIDADE DE AMOSTRAS BACTERIANAS EM SUPERFÍCIES ABIÓTICAS COM A INFLUÊNCIA DE FLUÍDOS BIOLÓGICOS Esteves, Deigilam Cestari viabilidade bacteriana superfícies abióticas fluídos biológicos contaminação ambiental Bacterial viability abiotic surfaces biological fluids environmental contamination CNPQ:: |
title_short |
AVALIAÇÃO DA VIABILIDADE DE AMOSTRAS BACTERIANAS EM SUPERFÍCIES ABIÓTICAS COM A INFLUÊNCIA DE FLUÍDOS BIOLÓGICOS |
title_full |
AVALIAÇÃO DA VIABILIDADE DE AMOSTRAS BACTERIANAS EM SUPERFÍCIES ABIÓTICAS COM A INFLUÊNCIA DE FLUÍDOS BIOLÓGICOS |
title_fullStr |
AVALIAÇÃO DA VIABILIDADE DE AMOSTRAS BACTERIANAS EM SUPERFÍCIES ABIÓTICAS COM A INFLUÊNCIA DE FLUÍDOS BIOLÓGICOS |
title_full_unstemmed |
AVALIAÇÃO DA VIABILIDADE DE AMOSTRAS BACTERIANAS EM SUPERFÍCIES ABIÓTICAS COM A INFLUÊNCIA DE FLUÍDOS BIOLÓGICOS |
title_sort |
AVALIAÇÃO DA VIABILIDADE DE AMOSTRAS BACTERIANAS EM SUPERFÍCIES ABIÓTICAS COM A INFLUÊNCIA DE FLUÍDOS BIOLÓGICOS |
author |
Esteves, Deigilam Cestari |
author_facet |
Esteves, Deigilam Cestari |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Rodrigues, Marcus Vinícius Pimenta |
dc.contributor.advisor1ID.fl_str_mv |
CPF:27602785819 |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/0133739307461180 |
dc.contributor.referee1.fl_str_mv |
Favareto, Ana Paula Alves |
dc.contributor.referee1ID.fl_str_mv |
CPF:31544492804 |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/2816211314670217 |
dc.contributor.referee2.fl_str_mv |
Martins, André |
dc.contributor.referee2ID.fl_str_mv |
CPF:29647935811 |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/9735485654252852 |
dc.contributor.authorID.fl_str_mv |
CPF:29039615810 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/8624795479325388 |
dc.contributor.author.fl_str_mv |
Esteves, Deigilam Cestari |
contributor_str_mv |
Rodrigues, Marcus Vinícius Pimenta Favareto, Ana Paula Alves Martins, André |
dc.subject.por.fl_str_mv |
viabilidade bacteriana superfícies abióticas fluídos biológicos contaminação ambiental |
topic |
viabilidade bacteriana superfícies abióticas fluídos biológicos contaminação ambiental Bacterial viability abiotic surfaces biological fluids environmental contamination CNPQ:: |
dc.subject.eng.fl_str_mv |
Bacterial viability abiotic surfaces biological fluids environmental contamination |
dc.subject.cnpq.fl_str_mv |
CNPQ:: |
description |
The environmental and hospital infections, caused by bacteria which are resistant to a wide variety of antibiotics, have shown increasing records in the last years, manifesting themselves in high mortality and lethality. Recent researches report that the bacteria presents a survival profile on dry surfaces in order to maintain their virulence when exposed to biological fluids such as urine, saliva and blood. The objective of this study was to document - through laboratorial analysis the survival capacity of the main bacteria of medical interest on abiotic surfaces. The adopted procedures were completely conducted in the microbiology laboratory at Unoeste, in Presidente Prudente SP. Standard ATCC strains of Staphylococcus aureus (ATCC25923), Enterococcus faecalis (ATCC29212), Escherichia coli (ATCC35218), Klebsiella pneumoniae (ATCC700603) and Pseudomonas aeruginosa (ATCC27583) were used, to which biological fluids and water were added. The surfaces were tile, synthetic fabric, mattress and cotton fabric. The surfaces were contaminated with suspension composed by the ATCC strains, biological fluids and water in addition to the control containing only the ATCC strains, which were stored in petri dishes, kept in room temperature. Every seven days the surfaces were dipped in trypticase soy broth and kept in the oven for 24h at 37ºC. They were spread from the contaminated brothin Mueller Hinton agar and kept for 24h in the oven at 37ºC. The viability analysis was done through the colony-forming unity (CFU) counting. For the statistical analysis, the Software R was used, through the Friedman and Kruskal Wallis tests for multiple comparisons, analyzing within each factor (solution or surface) which differ between one another (Friedman s test) and also which treatments interactions are different (Kruskal-Wallis). Through this analysis it was possible to observe that in the current work that Staphylococcus aureus kept its viability for a longer period than other microorganisms under all the tested conditions, presenting significant differences between the fluids and surfaces, with a particular colony growth in blood and cotton. The Klebsiella pneumoniae showed differences between fluids, with saliva containing the highest colony number. With regards to the other tested bacteria, there was no isolated significance. For the multiple comparisons analysis, only the Pseudomonas aeruginosa presented no significant difference between any pair of treatments, whereas the other bacteria presented significant differences between the correlations. The need to analyze the environmental impact of these microorganisms persistence in environments, which are vulnerable to human beings, guides the creation of measures in order to control the spread of pathogenic microorganisms. |
publishDate |
2014 |
dc.date.issued.fl_str_mv |
2014-12-18 |
dc.date.available.fl_str_mv |
2015-06-10 |
dc.date.accessioned.fl_str_mv |
2016-07-18T17:46:20Z |
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info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
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masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
ESTEVES, Deigilam Cestari. BACTERIAL SAMPLES OF FEASIBILITY ASSESSMENT SURFACE ABIOTIC WITH INFLUENCE BIOLOGICAL FLUIDS. 2014. 82 f. Dissertação (Mestrado em Ciências Ambientais) - Universidade do Oeste Paulista, Presidente Prudente, 2014. |
dc.identifier.uri.fl_str_mv |
http://bdtd.unoeste.br:8080/tede/handle/tede/490 |
identifier_str_mv |
ESTEVES, Deigilam Cestari. BACTERIAL SAMPLES OF FEASIBILITY ASSESSMENT SURFACE ABIOTIC WITH INFLUENCE BIOLOGICAL FLUIDS. 2014. 82 f. Dissertação (Mestrado em Ciências Ambientais) - Universidade do Oeste Paulista, Presidente Prudente, 2014. |
url |
http://bdtd.unoeste.br:8080/tede/handle/tede/490 |
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por |
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por |
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Universidade do Oeste Paulista |
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Mestrado em Meio Ambiente e Desenvolvimento Regional |
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UNOESTE |
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Ciências Ambientais |
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Universidade do Oeste Paulista |
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