Toxoplasma gondii em galinhas caipiras comercializadas em feiras-livres da região metropolitana de Recife, Pernambuco, Brasil
Autor(a) principal: | |
---|---|
Data de Publicação: | 2016 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da UFRPE |
Texto Completo: | http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/4477 |
Resumo: | Toxoplasmosis is a zoonosis, caused by the protozoan parasite Toxoplasma gondii, which represents an important public health burden worldwide. It has been estimated that 30% of the human population is seropositive for T. gondii. Severe complications may occur in immunocompromised patients or after congenital infection. Free-range chickens are considered an important host in the epidemiology of Toxoplasmosis, since they are a source of infection for cats and humans. The purpose of this study was to investigate the frequence and viability of T. gondii in free-range chickens destined for human consumption in Recife, Pernambuco state, Brazil. Serum samples from 56 free-range chickens were submitted to Immunofluorescence Antibody Test (IFAT) for detection of anti-T. gondii IgG antibodies. Tissue samples from seropositive chickens were submitted to PCR and mouse bioassay. From the tissue and serum of the mice submitted were performed PCR and IFAT, respectively. Anti-T. gondii IgG antibodies were found in 46.43% (26/56) of chickens and in 25% (13/52) of inoculated mice submitted to bioassay. Suggestive clinical manifestation of toxoplasmosis was observed in five inoculated mice, with one death. The data obtained in the present study confirm that free-range chicken meat is a potential source of T. gondii infection for humans and it has an important role in the toxoplasmosis chain transmission, revealing the need for implementation of prevention and control of toxoplasmosis. |
id |
URPE_7e1cddff89d6005c836eef3b531ce6b3 |
---|---|
oai_identifier_str |
oai:tede2:tede2/4477 |
network_acronym_str |
URPE |
network_name_str |
Biblioteca Digital de Teses e Dissertações da UFRPE |
repository_id_str |
|
spelling |
MOTA, Rinaldo AparecidoWANDERLEY, Flaviana SantosPORTO, Wagnner José NascimentoSÁ, Silvio Gomes dehttp://lattes.cnpq.br/2000352355407923ALCÂNTARA, Adrianne Mota de2016-05-20T13:52:26Z2016-02-19ALCÂNTARA, Adrianne Mota de. Toxoplasma gondii em galinhas caipiras comercializadas em feiras-livres da região metropolitana de Recife, Pernambuco, Brasil. 2016. 47 f. Dissertação (Programa de Pós-Graduação em Biociência Animal) - Universidade Federal Rural de Pernambuco, Recife.http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/4477Toxoplasmosis is a zoonosis, caused by the protozoan parasite Toxoplasma gondii, which represents an important public health burden worldwide. It has been estimated that 30% of the human population is seropositive for T. gondii. Severe complications may occur in immunocompromised patients or after congenital infection. Free-range chickens are considered an important host in the epidemiology of Toxoplasmosis, since they are a source of infection for cats and humans. The purpose of this study was to investigate the frequence and viability of T. gondii in free-range chickens destined for human consumption in Recife, Pernambuco state, Brazil. Serum samples from 56 free-range chickens were submitted to Immunofluorescence Antibody Test (IFAT) for detection of anti-T. gondii IgG antibodies. Tissue samples from seropositive chickens were submitted to PCR and mouse bioassay. From the tissue and serum of the mice submitted were performed PCR and IFAT, respectively. Anti-T. gondii IgG antibodies were found in 46.43% (26/56) of chickens and in 25% (13/52) of inoculated mice submitted to bioassay. Suggestive clinical manifestation of toxoplasmosis was observed in five inoculated mice, with one death. The data obtained in the present study confirm that free-range chicken meat is a potential source of T. gondii infection for humans and it has an important role in the toxoplasmosis chain transmission, revealing the need for implementation of prevention and control of toxoplasmosis.A toxoplasmose é uma importante zoonose que ocorre em todo o mundo, com uma estimativa de infecção de 30% da população humana, podendo ocorrer complicações em indivíduos imunocomprometidos e alterações neonatais em humanos. As aves domésticas são importantes hospedeiros intermediários de T. gondii por servirem de fonte de infecção do parasita para os felinos e seres humanos. O presente estudo teve como objetivo investigar a frequência e viabilidade de T. gondii em galinhas caipiras vendidas em feiras-livre de Recife – Pernambuco. Foram analisadas 56 amostras de soro de galinhas para a pesquisa de anticorpos IgG anti-T. gondii por meio da Reação de Imunofluorescência Indireta (RIFI). Amostras de tecidos das galinhas soropositivas foram submetidas à Reação em Cadeia da Polimerase (PCR) e ao bioensaio em camundongos. Foi realizada PCR e RIFI de tecido e soro dos camundongos submetidos ao bioensaio, respectivamente. Anticorpos IgG anti-T. gondii foram encontrados em 46,43% (26/56) das galinhas e em 25% (13/52) dos camundongos submetidos ao bioensaio. Sinais clínicos sugestivos de toxoplasmose foram observados em cinco dos camundongos inoculados e um óbito. Os resultados obtidos neste estudo demonstram que a carne e vísceras de galinhas caipiras podem atuar como uma fonte de infecção por T. gondii para humanos nesta região, reforçando a importância dessas aves na cadeia epidemiológica da toxoplasmose e a necessidade de implementação de medidas de prevenção e controle da enfermidade.Submitted by Mario BC (mario@bc.ufrpe.br) on 2016-05-20T13:52:26Z No. of bitstreams: 1 Adrianne Mota de Alcantara.pdf: 880772 bytes, checksum: d42422bf43cfe9ec3a3e1d6be2030d4e (MD5)Made available in DSpace on 2016-05-20T13:52:26Z (GMT). No. of bitstreams: 1 Adrianne Mota de Alcantara.pdf: 880772 bytes, checksum: d42422bf43cfe9ec3a3e1d6be2030d4e (MD5) Previous issue date: 2016-02-19Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESapplication/pdfporUniversidade Federal Rural de PernambucoPrograma de Pós-Graduação em Biociência AnimalUFRPEBrasilDepartamento de Morfologia e Fisiologia AnimalToxoplasmoseAveSaúde públicaGalinha caipiraBirdPublic healthCIENCIAS AGRARIAS::MEDICINA VETERINARIAToxoplasma gondii em galinhas caipiras comercializadas em feiras-livres da região metropolitana de Recife, Pernambuco, Brasilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis-1510757014399315592600600600600-89223641879873962044536702642350173193590462550136975366info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UFRPEinstname:Universidade Federal Rural de Pernambuco (UFRPE)instacron:UFRPELICENSElicense.txtlicense.txttext/plain; charset=utf-82089http://www.tede2.ufrpe.br:8080/tede2/bitstream/tede2/4477/1/license.txt7b5ba3d2445355f386edab96125d42b7MD51ORIGINALAdrianne Mota de Alcantara.pdfAdrianne Mota de Alcantara.pdfapplication/pdf880772http://www.tede2.ufrpe.br:8080/tede2/bitstream/tede2/4477/2/Adrianne+Mota+de+Alcantara.pdfd42422bf43cfe9ec3a3e1d6be2030d4eMD52tede2/44772023-06-12 15:23:50.367oai:tede2: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Biblioteca Digital de Teses e Dissertaçõeshttp://www.tede2.ufrpe.br:8080/tede/PUBhttp://www.tede2.ufrpe.br:8080/oai/requestbdtd@ufrpe.br ||bdtd@ufrpe.bropendoar:2023-06-12T18:23:50Biblioteca Digital de Teses e Dissertações da UFRPE - Universidade Federal Rural de Pernambuco (UFRPE)false |
dc.title.por.fl_str_mv |
Toxoplasma gondii em galinhas caipiras comercializadas em feiras-livres da região metropolitana de Recife, Pernambuco, Brasil |
title |
Toxoplasma gondii em galinhas caipiras comercializadas em feiras-livres da região metropolitana de Recife, Pernambuco, Brasil |
spellingShingle |
Toxoplasma gondii em galinhas caipiras comercializadas em feiras-livres da região metropolitana de Recife, Pernambuco, Brasil ALCÂNTARA, Adrianne Mota de Toxoplasmose Ave Saúde pública Galinha caipira Bird Public health CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
title_short |
Toxoplasma gondii em galinhas caipiras comercializadas em feiras-livres da região metropolitana de Recife, Pernambuco, Brasil |
title_full |
Toxoplasma gondii em galinhas caipiras comercializadas em feiras-livres da região metropolitana de Recife, Pernambuco, Brasil |
title_fullStr |
Toxoplasma gondii em galinhas caipiras comercializadas em feiras-livres da região metropolitana de Recife, Pernambuco, Brasil |
title_full_unstemmed |
Toxoplasma gondii em galinhas caipiras comercializadas em feiras-livres da região metropolitana de Recife, Pernambuco, Brasil |
title_sort |
Toxoplasma gondii em galinhas caipiras comercializadas em feiras-livres da região metropolitana de Recife, Pernambuco, Brasil |
author |
ALCÂNTARA, Adrianne Mota de |
author_facet |
ALCÂNTARA, Adrianne Mota de |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
MOTA, Rinaldo Aparecido |
dc.contributor.referee1.fl_str_mv |
WANDERLEY, Flaviana Santos |
dc.contributor.referee2.fl_str_mv |
PORTO, Wagnner José Nascimento |
dc.contributor.referee3.fl_str_mv |
SÁ, Silvio Gomes de |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/2000352355407923 |
dc.contributor.author.fl_str_mv |
ALCÂNTARA, Adrianne Mota de |
contributor_str_mv |
MOTA, Rinaldo Aparecido WANDERLEY, Flaviana Santos PORTO, Wagnner José Nascimento SÁ, Silvio Gomes de |
dc.subject.por.fl_str_mv |
Toxoplasmose Ave Saúde pública Galinha caipira |
topic |
Toxoplasmose Ave Saúde pública Galinha caipira Bird Public health CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
dc.subject.eng.fl_str_mv |
Bird Public health |
dc.subject.cnpq.fl_str_mv |
CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
description |
Toxoplasmosis is a zoonosis, caused by the protozoan parasite Toxoplasma gondii, which represents an important public health burden worldwide. It has been estimated that 30% of the human population is seropositive for T. gondii. Severe complications may occur in immunocompromised patients or after congenital infection. Free-range chickens are considered an important host in the epidemiology of Toxoplasmosis, since they are a source of infection for cats and humans. The purpose of this study was to investigate the frequence and viability of T. gondii in free-range chickens destined for human consumption in Recife, Pernambuco state, Brazil. Serum samples from 56 free-range chickens were submitted to Immunofluorescence Antibody Test (IFAT) for detection of anti-T. gondii IgG antibodies. Tissue samples from seropositive chickens were submitted to PCR and mouse bioassay. From the tissue and serum of the mice submitted were performed PCR and IFAT, respectively. Anti-T. gondii IgG antibodies were found in 46.43% (26/56) of chickens and in 25% (13/52) of inoculated mice submitted to bioassay. Suggestive clinical manifestation of toxoplasmosis was observed in five inoculated mice, with one death. The data obtained in the present study confirm that free-range chicken meat is a potential source of T. gondii infection for humans and it has an important role in the toxoplasmosis chain transmission, revealing the need for implementation of prevention and control of toxoplasmosis. |
publishDate |
2016 |
dc.date.accessioned.fl_str_mv |
2016-05-20T13:52:26Z |
dc.date.issued.fl_str_mv |
2016-02-19 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
ALCÂNTARA, Adrianne Mota de. Toxoplasma gondii em galinhas caipiras comercializadas em feiras-livres da região metropolitana de Recife, Pernambuco, Brasil. 2016. 47 f. Dissertação (Programa de Pós-Graduação em Biociência Animal) - Universidade Federal Rural de Pernambuco, Recife. |
dc.identifier.uri.fl_str_mv |
http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/4477 |
identifier_str_mv |
ALCÂNTARA, Adrianne Mota de. Toxoplasma gondii em galinhas caipiras comercializadas em feiras-livres da região metropolitana de Recife, Pernambuco, Brasil. 2016. 47 f. Dissertação (Programa de Pós-Graduação em Biociência Animal) - Universidade Federal Rural de Pernambuco, Recife. |
url |
http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/4477 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.program.fl_str_mv |
-1510757014399315592 |
dc.relation.confidence.fl_str_mv |
600 600 600 600 |
dc.relation.department.fl_str_mv |
-8922364187987396204 |
dc.relation.cnpq.fl_str_mv |
453670264235017319 |
dc.relation.sponsorship.fl_str_mv |
3590462550136975366 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal Rural de Pernambuco |
dc.publisher.program.fl_str_mv |
Programa de Pós-Graduação em Biociência Animal |
dc.publisher.initials.fl_str_mv |
UFRPE |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Departamento de Morfologia e Fisiologia Animal |
publisher.none.fl_str_mv |
Universidade Federal Rural de Pernambuco |
dc.source.none.fl_str_mv |
reponame:Biblioteca Digital de Teses e Dissertações da UFRPE instname:Universidade Federal Rural de Pernambuco (UFRPE) instacron:UFRPE |
instname_str |
Universidade Federal Rural de Pernambuco (UFRPE) |
instacron_str |
UFRPE |
institution |
UFRPE |
reponame_str |
Biblioteca Digital de Teses e Dissertações da UFRPE |
collection |
Biblioteca Digital de Teses e Dissertações da UFRPE |
bitstream.url.fl_str_mv |
http://www.tede2.ufrpe.br:8080/tede2/bitstream/tede2/4477/1/license.txt http://www.tede2.ufrpe.br:8080/tede2/bitstream/tede2/4477/2/Adrianne+Mota+de+Alcantara.pdf |
bitstream.checksum.fl_str_mv |
7b5ba3d2445355f386edab96125d42b7 d42422bf43cfe9ec3a3e1d6be2030d4e |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 |
repository.name.fl_str_mv |
Biblioteca Digital de Teses e Dissertações da UFRPE - Universidade Federal Rural de Pernambuco (UFRPE) |
repository.mail.fl_str_mv |
bdtd@ufrpe.br ||bdtd@ufrpe.br |
_version_ |
1791083562386063360 |