Avaliação da carga parasitária em cães com infecção natural Leishmania (Leishmania) infantum chagasi submetidos a tratamento experimental
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da UFRPE |
Texto Completo: | http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/4688 |
Resumo: | The Canine Visceral Leishmaniasis is a disease whose etiological agent in Brazil is the species of Leishmania (Leishmania) infantum chagasi, endemic in several regions, transmitted by insect-vector Lutzomyia longipalpis. The domestic dog is considered the most important parasite reservoir. This study aimed to evaluate the parasite load in dogs naturally infected with Leishmania (L.) infantum chagasi submitted to experimental treatment. For this purpose we used 18 asymptomatic dogs naturally infected with Leishmania (L.) infantum chagasi of both sexes, various ages submitted to treatment protocol with allopurinol 10 mg / kg 12h hours, domperidone 1 mg / kg every 24 hours during twelve months, associated with inactivated vaccine, three doses at 21 days interval by the subcutaneous route at a dilution of lyophilized to two parts of a diluent. The animals were submitted to clinical evaluation, blood tests, biochemical, immunohistochemistry of skin and monitoring through the Polymerase Chain Reaction in moments zero (M0), six months (M6) and twelve months (12M). Were observed significant remission of clinical signs in 100% (18/18) of the dogs in the post-treatment times, as well as standardization and improvement of haematological and biochemical parameters. In immunohistochemistry the skin, there was a significant reduction and elimination of parasites in the skin between times. PCR detected DNA of the parasite in skin samples and bone marrow after twelve months of treatment, with significant reduction between the pre- and post-treatment. The results of this study conclude that the treatment with allopurinol and domperidone associated with inactivated vaccine, to promote remission of clinical signs and reduction of parasite burden in animals with visceral leishmaniasis. |
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ALVES, Leucio CâmaraPEREIRA JÚNIOR, Júlio RodriguesFARIAS, Márcia Paula OliveiraPORTO, Wagnner José Nascimentohttp://lattes.cnpq.br/5553824684124218NASCIMENTO, Glaucia Grazielle2016-06-13T11:58:05Z2015-06-15NASCIMENTO, Glaucia Grazielle. Avaliação da carga parasitária em cães com infecção natural Leishmania (Leishmania) infantum chagasi submetidos a tratamento experimental. 2015. 88 f. Dissertação (Programa de Pós-Graduação em Ciência Animal Tropical) - Universidade Federal Rural de Pernambuco, Recife.http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/4688The Canine Visceral Leishmaniasis is a disease whose etiological agent in Brazil is the species of Leishmania (Leishmania) infantum chagasi, endemic in several regions, transmitted by insect-vector Lutzomyia longipalpis. The domestic dog is considered the most important parasite reservoir. This study aimed to evaluate the parasite load in dogs naturally infected with Leishmania (L.) infantum chagasi submitted to experimental treatment. For this purpose we used 18 asymptomatic dogs naturally infected with Leishmania (L.) infantum chagasi of both sexes, various ages submitted to treatment protocol with allopurinol 10 mg / kg 12h hours, domperidone 1 mg / kg every 24 hours during twelve months, associated with inactivated vaccine, three doses at 21 days interval by the subcutaneous route at a dilution of lyophilized to two parts of a diluent. The animals were submitted to clinical evaluation, blood tests, biochemical, immunohistochemistry of skin and monitoring through the Polymerase Chain Reaction in moments zero (M0), six months (M6) and twelve months (12M). Were observed significant remission of clinical signs in 100% (18/18) of the dogs in the post-treatment times, as well as standardization and improvement of haematological and biochemical parameters. In immunohistochemistry the skin, there was a significant reduction and elimination of parasites in the skin between times. PCR detected DNA of the parasite in skin samples and bone marrow after twelve months of treatment, with significant reduction between the pre- and post-treatment. The results of this study conclude that the treatment with allopurinol and domperidone associated with inactivated vaccine, to promote remission of clinical signs and reduction of parasite burden in animals with visceral leishmaniasis.A Leishmaniose Visceral Canina é uma doença que tem como agente etiológico no Brasil a espécie do protozoário Leishmania (Leishmania) infantum chagasi, endêmica em várias regiões, transmitida pelo inseto-vetor Lutzomyia longipalpis. O cão doméstico é considerado o mais importante reservatório do parasita. O objetivo deste trabalho foi avaliar a carga parasitária em cães naturalmente infectados com Leishmania (L.) infantum chagasi submetidos a tratamento experimental. Para tanto utilizou-se 18 cães sintomáticos, naturalmente infectados por Leishmania (L.) infantum chagasi de ambos os sexos, idades variadas submetidos a protocolo de tratamento com alopurinol 10 mg/kg a cada 12h horas, domperidona 1 mg/kg a cada 24 horas, durante doze meses, associado com a vacina inativada, três doses com intervalo de 21 dias, por via subcutânea, na diluição de duas partes do liofilizado para uma do diluente. Os animais foram submetidos à avaliação clínica, testes hematológicos, bioquímicos, imuno-histoquímica de pele e monitoramento através da Reação em Cadeia da Polimerase, nos momentos zero (M0), seis meses (M6) e doze meses (12M). Houve remissão significativa dos sinais clínicos em 100% (18/18) dos cães nos momentos pós-tratamento, bem como normalização e melhora dos parâmetros hematológicos e bioquímicos. Na imuno-histoquímica da pele, houve redução significativa e eliminação dos parasitos na pele entre os momentos. Na PCR foi detectado DNA do parasita nas amostras de pele e medula óssea após doze meses de tratamento, embora com redução significativas entre os momentos pré e pós-tratamento. Os resultados deste trabalho concluem que o tratamento com alopurinol e domperidona associado com a vacina inativada, promove a remissão dos sinais clinicos e redução da carga parasitária em animais com leishmaniose visceral.Submitted by Mario BC (mario@bc.ufrpe.br) on 2016-06-13T11:58:04Z No. of bitstreams: 1 Glaucia Grazielle Nascimento.pdf: 1563273 bytes, checksum: 8be5176981c6636746920cd3e2419ba1 (MD5)Made available in DSpace on 2016-06-13T11:58:05Z (GMT). No. of bitstreams: 1 Glaucia Grazielle Nascimento.pdf: 1563273 bytes, checksum: 8be5176981c6636746920cd3e2419ba1 (MD5) Previous issue date: 2015-06-15application/pdfporUniversidade Federal Rural de PernambucoPrograma de Pós-Graduação em Ciência Animal TropicalUFRPEBrasilDepartamento de Morfologia e Fisiologia AnimalLeishmaniaCãoCarga parasitáriaCIENCIAS AGRARIAS::MEDICINA VETERINARIAAvaliação da carga parasitária em cães com infecção natural Leishmania (Leishmania) infantum chagasi submetidos a tratamento experimentalParasite load evaluation in gogs naturally infected with Leishmania (Leishmania) infantum chagasi submitted to experimental treatmentinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis-6143187600765506511600600600-8922364187987396204453670264235017319info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UFRPEinstname:Universidade Federal Rural de Pernambuco (UFRPE)instacron:UFRPELICENSElicense.txtlicense.txttext/plain; charset=utf-82165http://www.tede2.ufrpe.br:8080/tede2/bitstream/tede2/4688/1/license.txtbd3efa91386c1718a7f26a329fdcb468MD51ORIGINALGlaucia Grazielle Nascimento.pdfGlaucia Grazielle Nascimento.pdfapplication/pdf1563273http://www.tede2.ufrpe.br:8080/tede2/bitstream/tede2/4688/2/Glaucia+Grazielle+Nascimento.pdf8be5176981c6636746920cd3e2419ba1MD52tede2/46882018-06-14 10:46:29.489oai:tede2: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Biblioteca Digital de Teses e Dissertaçõeshttp://www.tede2.ufrpe.br:8080/tede/PUBhttp://www.tede2.ufrpe.br:8080/oai/requestbdtd@ufrpe.br ||bdtd@ufrpe.bropendoar:2018-06-14T13:46:29Biblioteca Digital de Teses e Dissertações da UFRPE - Universidade Federal Rural de Pernambuco (UFRPE)false |
dc.title.por.fl_str_mv |
Avaliação da carga parasitária em cães com infecção natural Leishmania (Leishmania) infantum chagasi submetidos a tratamento experimental |
dc.title.alternative.eng.fl_str_mv |
Parasite load evaluation in gogs naturally infected with Leishmania (Leishmania) infantum chagasi submitted to experimental treatment |
title |
Avaliação da carga parasitária em cães com infecção natural Leishmania (Leishmania) infantum chagasi submetidos a tratamento experimental |
spellingShingle |
Avaliação da carga parasitária em cães com infecção natural Leishmania (Leishmania) infantum chagasi submetidos a tratamento experimental NASCIMENTO, Glaucia Grazielle Leishmania Cão Carga parasitária CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
title_short |
Avaliação da carga parasitária em cães com infecção natural Leishmania (Leishmania) infantum chagasi submetidos a tratamento experimental |
title_full |
Avaliação da carga parasitária em cães com infecção natural Leishmania (Leishmania) infantum chagasi submetidos a tratamento experimental |
title_fullStr |
Avaliação da carga parasitária em cães com infecção natural Leishmania (Leishmania) infantum chagasi submetidos a tratamento experimental |
title_full_unstemmed |
Avaliação da carga parasitária em cães com infecção natural Leishmania (Leishmania) infantum chagasi submetidos a tratamento experimental |
title_sort |
Avaliação da carga parasitária em cães com infecção natural Leishmania (Leishmania) infantum chagasi submetidos a tratamento experimental |
author |
NASCIMENTO, Glaucia Grazielle |
author_facet |
NASCIMENTO, Glaucia Grazielle |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
ALVES, Leucio Câmara |
dc.contributor.referee1.fl_str_mv |
PEREIRA JÚNIOR, Júlio Rodrigues |
dc.contributor.referee2.fl_str_mv |
FARIAS, Márcia Paula Oliveira |
dc.contributor.referee3.fl_str_mv |
PORTO, Wagnner José Nascimento |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/5553824684124218 |
dc.contributor.author.fl_str_mv |
NASCIMENTO, Glaucia Grazielle |
contributor_str_mv |
ALVES, Leucio Câmara PEREIRA JÚNIOR, Júlio Rodrigues FARIAS, Márcia Paula Oliveira PORTO, Wagnner José Nascimento |
dc.subject.por.fl_str_mv |
Leishmania Cão Carga parasitária |
topic |
Leishmania Cão Carga parasitária CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
dc.subject.cnpq.fl_str_mv |
CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
description |
The Canine Visceral Leishmaniasis is a disease whose etiological agent in Brazil is the species of Leishmania (Leishmania) infantum chagasi, endemic in several regions, transmitted by insect-vector Lutzomyia longipalpis. The domestic dog is considered the most important parasite reservoir. This study aimed to evaluate the parasite load in dogs naturally infected with Leishmania (L.) infantum chagasi submitted to experimental treatment. For this purpose we used 18 asymptomatic dogs naturally infected with Leishmania (L.) infantum chagasi of both sexes, various ages submitted to treatment protocol with allopurinol 10 mg / kg 12h hours, domperidone 1 mg / kg every 24 hours during twelve months, associated with inactivated vaccine, three doses at 21 days interval by the subcutaneous route at a dilution of lyophilized to two parts of a diluent. The animals were submitted to clinical evaluation, blood tests, biochemical, immunohistochemistry of skin and monitoring through the Polymerase Chain Reaction in moments zero (M0), six months (M6) and twelve months (12M). Were observed significant remission of clinical signs in 100% (18/18) of the dogs in the post-treatment times, as well as standardization and improvement of haematological and biochemical parameters. In immunohistochemistry the skin, there was a significant reduction and elimination of parasites in the skin between times. PCR detected DNA of the parasite in skin samples and bone marrow after twelve months of treatment, with significant reduction between the pre- and post-treatment. The results of this study conclude that the treatment with allopurinol and domperidone associated with inactivated vaccine, to promote remission of clinical signs and reduction of parasite burden in animals with visceral leishmaniasis. |
publishDate |
2015 |
dc.date.issued.fl_str_mv |
2015-06-15 |
dc.date.accessioned.fl_str_mv |
2016-06-13T11:58:05Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
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publishedVersion |
dc.identifier.citation.fl_str_mv |
NASCIMENTO, Glaucia Grazielle. Avaliação da carga parasitária em cães com infecção natural Leishmania (Leishmania) infantum chagasi submetidos a tratamento experimental. 2015. 88 f. Dissertação (Programa de Pós-Graduação em Ciência Animal Tropical) - Universidade Federal Rural de Pernambuco, Recife. |
dc.identifier.uri.fl_str_mv |
http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/4688 |
identifier_str_mv |
NASCIMENTO, Glaucia Grazielle. Avaliação da carga parasitária em cães com infecção natural Leishmania (Leishmania) infantum chagasi submetidos a tratamento experimental. 2015. 88 f. Dissertação (Programa de Pós-Graduação em Ciência Animal Tropical) - Universidade Federal Rural de Pernambuco, Recife. |
url |
http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/4688 |
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por |
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por |
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600 600 600 |
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openAccess |
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Universidade Federal Rural de Pernambuco |
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Programa de Pós-Graduação em Ciência Animal Tropical |
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UFRPE |
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Universidade Federal Rural de Pernambuco |
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