The sealing ability of MTA apical plugs exposed to a phosphate-buffered saline
Main Author: | |
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Publication Date: | 2013 |
Other Authors: | , , , , , |
Format: | Article |
Language: | eng |
Source: | Journal of applied oral science (Online) |
Download full: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572013000400341 |
Summary: | OBJECTIVE: The aim of this study was to evaluate the influence of exposure of the mineral trioxide aggregate (MTA) - with and without calcium chloride (CaCl2) - to phosphate-buffered saline (PBS) on the apical microleakage using a glucose leakage system. MATERIAL AND METHODS: Sixty root segments were randomly divided into 4 experimental groups (n=15). After resecting the apical segments and enlarging the canals with Gates-Glidden drills, the apical cavities were filled with MTA with or without CaCl2 and the root canals were dressed with a moistened cotton pellet or PBS, as follows: 1) MTA/cotton pellet; 2) MTA/PBS; 3) MTA+10%CaCl2/cotton pellet; 4) MTA+10%CaCl2/PBS. All root segments were introduced in floral foams moistened with PBS. After 2 months, all root segments were prepared to evaluate the glucose leakage along the apical plugs. The amount of glucose leakage was measured following an enzymatic reaction and quantified by a spectrophotometer. Four roots were used as controls. The data were analyzed using Kruskal-Wallis and Mann-Whitney tests (p<0.05). RESULTS: There were no differences between groups 1 and 2 (p>0.05), and 3 and 4 (p>0.05). The addition of CaCl2 to the MTA significantly decreased its sealing ability (p<0.05). CONCLUSION: The interaction with PBS did not improve the MTA sealing ability. The addition of CaCl2 to the MTA negatively influenced the apical seal. |
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The sealing ability of MTA apical plugs exposed to a phosphate-buffered salineSealing abilityApexificationGlucose OBJECTIVE: The aim of this study was to evaluate the influence of exposure of the mineral trioxide aggregate (MTA) - with and without calcium chloride (CaCl2) - to phosphate-buffered saline (PBS) on the apical microleakage using a glucose leakage system. MATERIAL AND METHODS: Sixty root segments were randomly divided into 4 experimental groups (n=15). After resecting the apical segments and enlarging the canals with Gates-Glidden drills, the apical cavities were filled with MTA with or without CaCl2 and the root canals were dressed with a moistened cotton pellet or PBS, as follows: 1) MTA/cotton pellet; 2) MTA/PBS; 3) MTA+10%CaCl2/cotton pellet; 4) MTA+10%CaCl2/PBS. All root segments were introduced in floral foams moistened with PBS. After 2 months, all root segments were prepared to evaluate the glucose leakage along the apical plugs. The amount of glucose leakage was measured following an enzymatic reaction and quantified by a spectrophotometer. Four roots were used as controls. The data were analyzed using Kruskal-Wallis and Mann-Whitney tests (p<0.05). RESULTS: There were no differences between groups 1 and 2 (p>0.05), and 3 and 4 (p>0.05). The addition of CaCl2 to the MTA significantly decreased its sealing ability (p<0.05). CONCLUSION: The interaction with PBS did not improve the MTA sealing ability. The addition of CaCl2 to the MTA negatively influenced the apical seal. Faculdade De Odontologia De Bauru - USP2013-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572013000400341Journal of Applied Oral Science v.21 n.4 2013reponame:Journal of applied oral science (Online)instname:Universidade de São Paulo (USP)instacron:USP10.1590/1678-775720130023info:eu-repo/semantics/openAccessAlmeida,Josiane deAlves,Ana Maria HeckeMelo,Roberto Ferreira deFelippe,Mara Cristina SantosBortoluzzi,Eduardo AntunesTeixeira,Cleonice da SilveiraFelippe,Wilson Tadeueng2013-10-10T00:00:00Zoai:scielo:S1678-77572013000400341Revistahttp://www.scielo.br/jaosPUBhttps://old.scielo.br/oai/scielo-oai.php||jaos@usp.br1678-77651678-7757opendoar:2013-10-10T00:00Journal of applied oral science (Online) - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
The sealing ability of MTA apical plugs exposed to a phosphate-buffered saline |
title |
The sealing ability of MTA apical plugs exposed to a phosphate-buffered saline |
spellingShingle |
The sealing ability of MTA apical plugs exposed to a phosphate-buffered saline Almeida,Josiane de Sealing ability Apexification Glucose |
title_short |
The sealing ability of MTA apical plugs exposed to a phosphate-buffered saline |
title_full |
The sealing ability of MTA apical plugs exposed to a phosphate-buffered saline |
title_fullStr |
The sealing ability of MTA apical plugs exposed to a phosphate-buffered saline |
title_full_unstemmed |
The sealing ability of MTA apical plugs exposed to a phosphate-buffered saline |
title_sort |
The sealing ability of MTA apical plugs exposed to a phosphate-buffered saline |
author |
Almeida,Josiane de |
author_facet |
Almeida,Josiane de Alves,Ana Maria Hecke Melo,Roberto Ferreira de Felippe,Mara Cristina Santos Bortoluzzi,Eduardo Antunes Teixeira,Cleonice da Silveira Felippe,Wilson Tadeu |
author_role |
author |
author2 |
Alves,Ana Maria Hecke Melo,Roberto Ferreira de Felippe,Mara Cristina Santos Bortoluzzi,Eduardo Antunes Teixeira,Cleonice da Silveira Felippe,Wilson Tadeu |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Almeida,Josiane de Alves,Ana Maria Hecke Melo,Roberto Ferreira de Felippe,Mara Cristina Santos Bortoluzzi,Eduardo Antunes Teixeira,Cleonice da Silveira Felippe,Wilson Tadeu |
dc.subject.por.fl_str_mv |
Sealing ability Apexification Glucose |
topic |
Sealing ability Apexification Glucose |
description |
OBJECTIVE: The aim of this study was to evaluate the influence of exposure of the mineral trioxide aggregate (MTA) - with and without calcium chloride (CaCl2) - to phosphate-buffered saline (PBS) on the apical microleakage using a glucose leakage system. MATERIAL AND METHODS: Sixty root segments were randomly divided into 4 experimental groups (n=15). After resecting the apical segments and enlarging the canals with Gates-Glidden drills, the apical cavities were filled with MTA with or without CaCl2 and the root canals were dressed with a moistened cotton pellet or PBS, as follows: 1) MTA/cotton pellet; 2) MTA/PBS; 3) MTA+10%CaCl2/cotton pellet; 4) MTA+10%CaCl2/PBS. All root segments were introduced in floral foams moistened with PBS. After 2 months, all root segments were prepared to evaluate the glucose leakage along the apical plugs. The amount of glucose leakage was measured following an enzymatic reaction and quantified by a spectrophotometer. Four roots were used as controls. The data were analyzed using Kruskal-Wallis and Mann-Whitney tests (p<0.05). RESULTS: There were no differences between groups 1 and 2 (p>0.05), and 3 and 4 (p>0.05). The addition of CaCl2 to the MTA significantly decreased its sealing ability (p<0.05). CONCLUSION: The interaction with PBS did not improve the MTA sealing ability. The addition of CaCl2 to the MTA negatively influenced the apical seal. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-08-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572013000400341 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572013000400341 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1678-775720130023 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Faculdade De Odontologia De Bauru - USP |
publisher.none.fl_str_mv |
Faculdade De Odontologia De Bauru - USP |
dc.source.none.fl_str_mv |
Journal of Applied Oral Science v.21 n.4 2013 reponame:Journal of applied oral science (Online) instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Journal of applied oral science (Online) |
collection |
Journal of applied oral science (Online) |
repository.name.fl_str_mv |
Journal of applied oral science (Online) - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
||jaos@usp.br |
_version_ |
1748936437640724480 |