Quality of cellular attachment to various root-end filling materials
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Journal of applied oral science (Online) |
Texto Completo: | https://www.revistas.usp.br/jaos/article/view/3990 |
Resumo: | OBJECTIVES: This study investigated cellular attachment to 6 root-end filling materials as a measure of the biocompatibility of the materials. MATERIAL AND METHODS: Class I retrograde cavities were prepared in root slices and these cavities were filled with the test materials, and incubated with Balb/C 3T3 fibroblasts for 24 h. Root slices with the cavities left empty served as the controls. The root slices were then processed for scanning electron microscopy, and were viewed to assess the quality of cellular attachment by observing the shape of cells, spread, and membrane outline. RESULTS: The best cellular attachment was observed at MTA and Geristore surfaces: cells exhibited characteristic elongated fibroblastic morphology, with projections of lamellipodia, filopodia, blebs, and microvilli from their surfaces, reflecting good attachment to the material. Fibroblasts attached poorly to the surfaces of IRM, Super EBA, KetacFil and Retroplast. Furthermore, the cells did not attach well to the tooth structure next to IRM and Super EBA. CONCLUSIONS: The present study demonstrated a variation in cellular attachment to different root-end filling materials with the best cellular attachment to the surfaces of MTA and Geristore. IRM and Super EBA, Ketac Fil and Retroplast rendered poor attachment. |
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Journal of applied oral science (Online) |
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Quality of cellular attachment to various root-end filling materials Biocompatibility testingCell adhesionElectron microscopyEndodonticsDental materialsFibroblasts OBJECTIVES: This study investigated cellular attachment to 6 root-end filling materials as a measure of the biocompatibility of the materials. MATERIAL AND METHODS: Class I retrograde cavities were prepared in root slices and these cavities were filled with the test materials, and incubated with Balb/C 3T3 fibroblasts for 24 h. Root slices with the cavities left empty served as the controls. The root slices were then processed for scanning electron microscopy, and were viewed to assess the quality of cellular attachment by observing the shape of cells, spread, and membrane outline. RESULTS: The best cellular attachment was observed at MTA and Geristore surfaces: cells exhibited characteristic elongated fibroblastic morphology, with projections of lamellipodia, filopodia, blebs, and microvilli from their surfaces, reflecting good attachment to the material. Fibroblasts attached poorly to the surfaces of IRM, Super EBA, KetacFil and Retroplast. Furthermore, the cells did not attach well to the tooth structure next to IRM and Super EBA. CONCLUSIONS: The present study demonstrated a variation in cellular attachment to different root-end filling materials with the best cellular attachment to the surfaces of MTA and Geristore. IRM and Super EBA, Ketac Fil and Retroplast rendered poor attachment. Universidade de São Paulo. Faculdade de Odontologia de Bauru2012-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/jaos/article/view/399010.1590/S1678-77572012000100015Journal of Applied Oral Science; Vol. 20 No. 1 (2012); 82-88 Journal of Applied Oral Science; Vol. 20 Núm. 1 (2012); 82-88 Journal of Applied Oral Science; v. 20 n. 1 (2012); 82-88 1678-77651678-7757reponame:Journal of applied oral science (Online)instname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/jaos/article/view/3990/4680Copyright (c) 2012 Journal of Applied Oral Scienceinfo:eu-repo/semantics/openAccessAL-Hiyasat, Ahmad S.Al-Sa'Eed, Oula R.Darmani, Homa2012-04-27T12:17:47Zoai:revistas.usp.br:article/3990Revistahttp://www.scielo.br/jaosPUBhttps://www.revistas.usp.br/jaos/oai||jaos@usp.br1678-77651678-7757opendoar:2012-04-27T12:17:47Journal of applied oral science (Online) - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Quality of cellular attachment to various root-end filling materials |
title |
Quality of cellular attachment to various root-end filling materials |
spellingShingle |
Quality of cellular attachment to various root-end filling materials AL-Hiyasat, Ahmad S. Biocompatibility testing Cell adhesion Electron microscopy Endodontics Dental materials Fibroblasts |
title_short |
Quality of cellular attachment to various root-end filling materials |
title_full |
Quality of cellular attachment to various root-end filling materials |
title_fullStr |
Quality of cellular attachment to various root-end filling materials |
title_full_unstemmed |
Quality of cellular attachment to various root-end filling materials |
title_sort |
Quality of cellular attachment to various root-end filling materials |
author |
AL-Hiyasat, Ahmad S. |
author_facet |
AL-Hiyasat, Ahmad S. Al-Sa'Eed, Oula R. Darmani, Homa |
author_role |
author |
author2 |
Al-Sa'Eed, Oula R. Darmani, Homa |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
AL-Hiyasat, Ahmad S. Al-Sa'Eed, Oula R. Darmani, Homa |
dc.subject.por.fl_str_mv |
Biocompatibility testing Cell adhesion Electron microscopy Endodontics Dental materials Fibroblasts |
topic |
Biocompatibility testing Cell adhesion Electron microscopy Endodontics Dental materials Fibroblasts |
description |
OBJECTIVES: This study investigated cellular attachment to 6 root-end filling materials as a measure of the biocompatibility of the materials. MATERIAL AND METHODS: Class I retrograde cavities were prepared in root slices and these cavities were filled with the test materials, and incubated with Balb/C 3T3 fibroblasts for 24 h. Root slices with the cavities left empty served as the controls. The root slices were then processed for scanning electron microscopy, and were viewed to assess the quality of cellular attachment by observing the shape of cells, spread, and membrane outline. RESULTS: The best cellular attachment was observed at MTA and Geristore surfaces: cells exhibited characteristic elongated fibroblastic morphology, with projections of lamellipodia, filopodia, blebs, and microvilli from their surfaces, reflecting good attachment to the material. Fibroblasts attached poorly to the surfaces of IRM, Super EBA, KetacFil and Retroplast. Furthermore, the cells did not attach well to the tooth structure next to IRM and Super EBA. CONCLUSIONS: The present study demonstrated a variation in cellular attachment to different root-end filling materials with the best cellular attachment to the surfaces of MTA and Geristore. IRM and Super EBA, Ketac Fil and Retroplast rendered poor attachment. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-02-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/jaos/article/view/3990 10.1590/S1678-77572012000100015 |
url |
https://www.revistas.usp.br/jaos/article/view/3990 |
identifier_str_mv |
10.1590/S1678-77572012000100015 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/jaos/article/view/3990/4680 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2012 Journal of Applied Oral Science info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2012 Journal of Applied Oral Science |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Odontologia de Bauru |
publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Odontologia de Bauru |
dc.source.none.fl_str_mv |
Journal of Applied Oral Science; Vol. 20 No. 1 (2012); 82-88 Journal of Applied Oral Science; Vol. 20 Núm. 1 (2012); 82-88 Journal of Applied Oral Science; v. 20 n. 1 (2012); 82-88 1678-7765 1678-7757 reponame:Journal of applied oral science (Online) instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Journal of applied oral science (Online) |
collection |
Journal of applied oral science (Online) |
repository.name.fl_str_mv |
Journal of applied oral science (Online) - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
||jaos@usp.br |
_version_ |
1787713192848261120 |