Effect of candesartan on the expression of sclera-choroidal intercellular adhesion molecule-1 in hypercholesterolemic models

Detalhes bibliográficos
Autor(a) principal: Torres, Rogil Jose de Almeida
Data de Publicação: 2014
Outros Autores: Luchini, Andrea, Torres, Regiane do Rocio de Almeida, Oliveira, Luciano Rodrigo Silva de, Torres, Caroline Luzia de Almeida, Torres, Robson Antonio de Almeida, Olandoski, Marcia, Nagashima, Seigo, Noronha, Lucia de, Precoma, Dalton Bertolim
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Clinics
Texto Completo: https://www.revistas.usp.br/clinics/article/view/77098
Resumo: OBJECTIVE: To evaluate the effect of blocking the angiotensin II AT-1 receptor by the systemic administration of candesartan on the expression of intercellular adhesion molecule-1 in the sclera and choroid of hypercholesterolemic rabbits. METHODS: New Zealand rabbits were divided into 3 groups, as follows: GI, which was fed a rabbit standard diet; GII, which was fed a hypercholesterolemic diet; and GIII, which received hypercholesterolemic diet plus candesartan. Samples of the rabbits' sclera and choroid were then studied by hematoxylin-eosin staining and histomorphometric and immunohistochemical analyses for intercellular adhesion molecule-1 expression. RESULTS: Histological analysis of hematoxylin- and eosin-stained sclera and choroid revealed that macrophages were rarely present in GI, and GII had significantly increased macrophage numbers compared to GIII. Moreover, in GII, the sclera and choroid morphometry showed a significant increase in thickness in comparison to GI and GIII. GIII presented a significant increase in thickness in relation to GI. Sclera and choroid immunohistochemical analysis for intercellular adhesion molecule-1 expression revealed a significant increase in immunoreactivity in GII in relation to GI and GIII. GIII showed a significant increase in immunoreactivity in relation to GI. CONCLUSION: Candesartan reduced the expression of intercellular adhesion molecule-1 and consequently macrophage accumulation in the sclera and choroid of hypercholesterolemic rabbits.
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spelling Effect of candesartan on the expression of sclera-choroidal intercellular adhesion molecule-1 in hypercholesterolemic modelsOBJECTIVE: To evaluate the effect of blocking the angiotensin II AT-1 receptor by the systemic administration of candesartan on the expression of intercellular adhesion molecule-1 in the sclera and choroid of hypercholesterolemic rabbits. METHODS: New Zealand rabbits were divided into 3 groups, as follows: GI, which was fed a rabbit standard diet; GII, which was fed a hypercholesterolemic diet; and GIII, which received hypercholesterolemic diet plus candesartan. Samples of the rabbits' sclera and choroid were then studied by hematoxylin-eosin staining and histomorphometric and immunohistochemical analyses for intercellular adhesion molecule-1 expression. RESULTS: Histological analysis of hematoxylin- and eosin-stained sclera and choroid revealed that macrophages were rarely present in GI, and GII had significantly increased macrophage numbers compared to GIII. Moreover, in GII, the sclera and choroid morphometry showed a significant increase in thickness in comparison to GI and GIII. GIII presented a significant increase in thickness in relation to GI. Sclera and choroid immunohistochemical analysis for intercellular adhesion molecule-1 expression revealed a significant increase in immunoreactivity in GII in relation to GI and GIII. GIII showed a significant increase in immunoreactivity in relation to GI. CONCLUSION: Candesartan reduced the expression of intercellular adhesion molecule-1 and consequently macrophage accumulation in the sclera and choroid of hypercholesterolemic rabbits.Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo2014-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/clinics/article/view/7709810.1590/clin.v69i2.77098Clinics; Vol. 69 No. 2 (2014); 145-149Clinics; v. 69 n. 2 (2014); 145-149Clinics; Vol. 69 Núm. 2 (2014); 145-1491980-53221807-5932reponame:Clinicsinstname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/clinics/article/view/77098/80965Torres, Rogil Jose de AlmeidaLuchini, AndreaTorres, Regiane do Rocio de AlmeidaOliveira, Luciano Rodrigo Silva deTorres, Caroline Luzia de AlmeidaTorres, Robson Antonio de AlmeidaOlandoski, MarciaNagashima, SeigoNoronha, Lucia dePrecoma, Dalton Bertoliminfo:eu-repo/semantics/openAccess2014-03-24T12:27:22Zoai:revistas.usp.br:article/77098Revistahttps://www.revistas.usp.br/clinicsPUBhttps://www.revistas.usp.br/clinics/oai||clinics@hc.fm.usp.br1980-53221807-5932opendoar:2014-03-24T12:27:22Clinics - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv Effect of candesartan on the expression of sclera-choroidal intercellular adhesion molecule-1 in hypercholesterolemic models
title Effect of candesartan on the expression of sclera-choroidal intercellular adhesion molecule-1 in hypercholesterolemic models
spellingShingle Effect of candesartan on the expression of sclera-choroidal intercellular adhesion molecule-1 in hypercholesterolemic models
Torres, Rogil Jose de Almeida
title_short Effect of candesartan on the expression of sclera-choroidal intercellular adhesion molecule-1 in hypercholesterolemic models
title_full Effect of candesartan on the expression of sclera-choroidal intercellular adhesion molecule-1 in hypercholesterolemic models
title_fullStr Effect of candesartan on the expression of sclera-choroidal intercellular adhesion molecule-1 in hypercholesterolemic models
title_full_unstemmed Effect of candesartan on the expression of sclera-choroidal intercellular adhesion molecule-1 in hypercholesterolemic models
title_sort Effect of candesartan on the expression of sclera-choroidal intercellular adhesion molecule-1 in hypercholesterolemic models
author Torres, Rogil Jose de Almeida
author_facet Torres, Rogil Jose de Almeida
Luchini, Andrea
Torres, Regiane do Rocio de Almeida
Oliveira, Luciano Rodrigo Silva de
Torres, Caroline Luzia de Almeida
Torres, Robson Antonio de Almeida
Olandoski, Marcia
Nagashima, Seigo
Noronha, Lucia de
Precoma, Dalton Bertolim
author_role author
author2 Luchini, Andrea
Torres, Regiane do Rocio de Almeida
Oliveira, Luciano Rodrigo Silva de
Torres, Caroline Luzia de Almeida
Torres, Robson Antonio de Almeida
Olandoski, Marcia
Nagashima, Seigo
Noronha, Lucia de
Precoma, Dalton Bertolim
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Torres, Rogil Jose de Almeida
Luchini, Andrea
Torres, Regiane do Rocio de Almeida
Oliveira, Luciano Rodrigo Silva de
Torres, Caroline Luzia de Almeida
Torres, Robson Antonio de Almeida
Olandoski, Marcia
Nagashima, Seigo
Noronha, Lucia de
Precoma, Dalton Bertolim
description OBJECTIVE: To evaluate the effect of blocking the angiotensin II AT-1 receptor by the systemic administration of candesartan on the expression of intercellular adhesion molecule-1 in the sclera and choroid of hypercholesterolemic rabbits. METHODS: New Zealand rabbits were divided into 3 groups, as follows: GI, which was fed a rabbit standard diet; GII, which was fed a hypercholesterolemic diet; and GIII, which received hypercholesterolemic diet plus candesartan. Samples of the rabbits' sclera and choroid were then studied by hematoxylin-eosin staining and histomorphometric and immunohistochemical analyses for intercellular adhesion molecule-1 expression. RESULTS: Histological analysis of hematoxylin- and eosin-stained sclera and choroid revealed that macrophages were rarely present in GI, and GII had significantly increased macrophage numbers compared to GIII. Moreover, in GII, the sclera and choroid morphometry showed a significant increase in thickness in comparison to GI and GIII. GIII presented a significant increase in thickness in relation to GI. Sclera and choroid immunohistochemical analysis for intercellular adhesion molecule-1 expression revealed a significant increase in immunoreactivity in GII in relation to GI and GIII. GIII showed a significant increase in immunoreactivity in relation to GI. CONCLUSION: Candesartan reduced the expression of intercellular adhesion molecule-1 and consequently macrophage accumulation in the sclera and choroid of hypercholesterolemic rabbits.
publishDate 2014
dc.date.none.fl_str_mv 2014-02-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/clinics/article/view/77098
10.1590/clin.v69i2.77098
url https://www.revistas.usp.br/clinics/article/view/77098
identifier_str_mv 10.1590/clin.v69i2.77098
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/clinics/article/view/77098/80965
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo
publisher.none.fl_str_mv Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo
dc.source.none.fl_str_mv Clinics; Vol. 69 No. 2 (2014); 145-149
Clinics; v. 69 n. 2 (2014); 145-149
Clinics; Vol. 69 Núm. 2 (2014); 145-149
1980-5322
1807-5932
reponame:Clinics
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
instacron_str USP
institution USP
reponame_str Clinics
collection Clinics
repository.name.fl_str_mv Clinics - Universidade de São Paulo (USP)
repository.mail.fl_str_mv ||clinics@hc.fm.usp.br
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