Morphoanatomical aspects of the starting material for the improvement of pineapple cryopreservation by the droplet-vitrification technique
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Anais da Academia Brasileira de Ciências (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652021000101501 |
Resumo: | Abstract Cryopreservation of pineapple shoot tips has been established from various protocols, including droplet vitrification. Thus, this work aimed to evaluate the morphoanatomical conditions of the starting material over different times (30, 45 and 60 days) of culture before freezing and its correlation with the survival percentage of the cryopreserved shoot tips. Four accessions, Ananas comosus var. comosus (BGA-009); var. bracteatus (BGA-119); var. parguazensis (BGA-376), var. erectifolius (BGA-750) from the Pineapple Active Germplasm Bank (BGA Pineapple) and two hybrids from the Embrapa Genetic Breeding Program, FIB-ROX1 (var. bracteatus X var. erectifolius) and FIB-ROX2 ( var. erectifolius X var. bracteatus), recently introduced in the field from in vitro storage, were used. Histological sections before freezing and the percentages of survival after freezing were obtained taking into account the different times of cultivation of the donor plants. The results showed a significative interaction between genotypes (accessions and hybrids) and the culture period. The accessions BGA-009 and BGA-119 showed the highest survival rates, with 95% and 90% respectively for the 30-day culture time. Different results were obtained for each genotype, showing the need for improvements in the standardization of starting material, which would allow better repeatability of the protocol. |
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Morphoanatomical aspects of the starting material for the improvement of pineapple cryopreservation by the droplet-vitrification techniqueAnanas comosus (L.) MerrilEx situ conservationGrowing timeStarting materialRegenerationAbstract Cryopreservation of pineapple shoot tips has been established from various protocols, including droplet vitrification. Thus, this work aimed to evaluate the morphoanatomical conditions of the starting material over different times (30, 45 and 60 days) of culture before freezing and its correlation with the survival percentage of the cryopreserved shoot tips. Four accessions, Ananas comosus var. comosus (BGA-009); var. bracteatus (BGA-119); var. parguazensis (BGA-376), var. erectifolius (BGA-750) from the Pineapple Active Germplasm Bank (BGA Pineapple) and two hybrids from the Embrapa Genetic Breeding Program, FIB-ROX1 (var. bracteatus X var. erectifolius) and FIB-ROX2 ( var. erectifolius X var. bracteatus), recently introduced in the field from in vitro storage, were used. Histological sections before freezing and the percentages of survival after freezing were obtained taking into account the different times of cultivation of the donor plants. The results showed a significative interaction between genotypes (accessions and hybrids) and the culture period. The accessions BGA-009 and BGA-119 showed the highest survival rates, with 95% and 90% respectively for the 30-day culture time. Different results were obtained for each genotype, showing the need for improvements in the standardization of starting material, which would allow better repeatability of the protocol.Academia Brasileira de Ciências2021-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652021000101501Anais da Academia Brasileira de Ciências v.93 n.1 2021reponame:Anais da Academia Brasileira de Ciências (Online)instname:Academia Brasileira de Ciências (ABC)instacron:ABC10.1590/0001-3765202120190555info:eu-repo/semantics/openAccessGUERRA,PATRÍCIA A.SOUZA,EVERTON H.MAX,DANIELA A. S.ROSSI,MÔNICA L.VILLALOBOS-OLIVERA,ARIELLEDO,CARLOS A. S.MARTINEZ-MONTERO,MARCOS E.SOUZA,FERNANDA V. D.eng2021-03-03T00:00:00Zoai:scielo:S0001-37652021000101501Revistahttp://www.scielo.br/aabchttps://old.scielo.br/oai/scielo-oai.php||aabc@abc.org.br1678-26900001-3765opendoar:2021-03-03T00:00Anais da Academia Brasileira de Ciências (Online) - Academia Brasileira de Ciências (ABC)false |
dc.title.none.fl_str_mv |
Morphoanatomical aspects of the starting material for the improvement of pineapple cryopreservation by the droplet-vitrification technique |
title |
Morphoanatomical aspects of the starting material for the improvement of pineapple cryopreservation by the droplet-vitrification technique |
spellingShingle |
Morphoanatomical aspects of the starting material for the improvement of pineapple cryopreservation by the droplet-vitrification technique GUERRA,PATRÍCIA A. Ananas comosus (L.) Merril Ex situ conservation Growing time Starting material Regeneration |
title_short |
Morphoanatomical aspects of the starting material for the improvement of pineapple cryopreservation by the droplet-vitrification technique |
title_full |
Morphoanatomical aspects of the starting material for the improvement of pineapple cryopreservation by the droplet-vitrification technique |
title_fullStr |
Morphoanatomical aspects of the starting material for the improvement of pineapple cryopreservation by the droplet-vitrification technique |
title_full_unstemmed |
Morphoanatomical aspects of the starting material for the improvement of pineapple cryopreservation by the droplet-vitrification technique |
title_sort |
Morphoanatomical aspects of the starting material for the improvement of pineapple cryopreservation by the droplet-vitrification technique |
author |
GUERRA,PATRÍCIA A. |
author_facet |
GUERRA,PATRÍCIA A. SOUZA,EVERTON H. MAX,DANIELA A. S. ROSSI,MÔNICA L. VILLALOBOS-OLIVERA,ARIEL LEDO,CARLOS A. S. MARTINEZ-MONTERO,MARCOS E. SOUZA,FERNANDA V. D. |
author_role |
author |
author2 |
SOUZA,EVERTON H. MAX,DANIELA A. S. ROSSI,MÔNICA L. VILLALOBOS-OLIVERA,ARIEL LEDO,CARLOS A. S. MARTINEZ-MONTERO,MARCOS E. SOUZA,FERNANDA V. D. |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
GUERRA,PATRÍCIA A. SOUZA,EVERTON H. MAX,DANIELA A. S. ROSSI,MÔNICA L. VILLALOBOS-OLIVERA,ARIEL LEDO,CARLOS A. S. MARTINEZ-MONTERO,MARCOS E. SOUZA,FERNANDA V. D. |
dc.subject.por.fl_str_mv |
Ananas comosus (L.) Merril Ex situ conservation Growing time Starting material Regeneration |
topic |
Ananas comosus (L.) Merril Ex situ conservation Growing time Starting material Regeneration |
description |
Abstract Cryopreservation of pineapple shoot tips has been established from various protocols, including droplet vitrification. Thus, this work aimed to evaluate the morphoanatomical conditions of the starting material over different times (30, 45 and 60 days) of culture before freezing and its correlation with the survival percentage of the cryopreserved shoot tips. Four accessions, Ananas comosus var. comosus (BGA-009); var. bracteatus (BGA-119); var. parguazensis (BGA-376), var. erectifolius (BGA-750) from the Pineapple Active Germplasm Bank (BGA Pineapple) and two hybrids from the Embrapa Genetic Breeding Program, FIB-ROX1 (var. bracteatus X var. erectifolius) and FIB-ROX2 ( var. erectifolius X var. bracteatus), recently introduced in the field from in vitro storage, were used. Histological sections before freezing and the percentages of survival after freezing were obtained taking into account the different times of cultivation of the donor plants. The results showed a significative interaction between genotypes (accessions and hybrids) and the culture period. The accessions BGA-009 and BGA-119 showed the highest survival rates, with 95% and 90% respectively for the 30-day culture time. Different results were obtained for each genotype, showing the need for improvements in the standardization of starting material, which would allow better repeatability of the protocol. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652021000101501 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652021000101501 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/0001-3765202120190555 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Academia Brasileira de Ciências |
publisher.none.fl_str_mv |
Academia Brasileira de Ciências |
dc.source.none.fl_str_mv |
Anais da Academia Brasileira de Ciências v.93 n.1 2021 reponame:Anais da Academia Brasileira de Ciências (Online) instname:Academia Brasileira de Ciências (ABC) instacron:ABC |
instname_str |
Academia Brasileira de Ciências (ABC) |
instacron_str |
ABC |
institution |
ABC |
reponame_str |
Anais da Academia Brasileira de Ciências (Online) |
collection |
Anais da Academia Brasileira de Ciências (Online) |
repository.name.fl_str_mv |
Anais da Academia Brasileira de Ciências (Online) - Academia Brasileira de Ciências (ABC) |
repository.mail.fl_str_mv |
||aabc@abc.org.br |
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1754302869740191744 |