Bioactivity and action mechanism of green propolis against Pythium aphanidermatum

Detalhes bibliográficos
Autor(a) principal: PAZIN,WALLANCE M.
Data de Publicação: 2019
Outros Autores: SANTOS,SUIKINAI N. DOS, QUEIROZ,SONIA C.N., BAGATOLLI,LUIS A., SOARES,ADEMILSON E.E., MELO,ITAMAR S. DE, ITO,AMANDO S.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Anais da Academia Brasileira de Ciências (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000300617
Resumo: Abstract: We have established how natural compounds from green propolis collected by the species Apis mellifera act against the growth of Pythium aphanidermatum. On the basis of mass spectrometry (Q-ToF MS), we determined that Artepillin C, the major constituent of green propolis, underlies the effect and displays activity against P. aphanidermatum at a minimal inhibitory concentration of 750 µg.mL-1. Biophysical studies based on model membranes showed that this inhibitory effect may be linked with a membrane-related phenomenon: Artepillin C increases the permeability of membranes with relatively high fluidity in their lateral structure, a feature that is in line with the lipid composition reported for the cytoplasmic membrane of P. aphanidermatum. Therefore, the present study supports the use of the effective and inexpensive green propolis to control the impact of the dangerous phytopathogen P. aphanidermatum on agriculture.
id ABC-1_62c79676625e3d5df9befda75bc44359
oai_identifier_str oai:scielo:S0001-37652019000300617
network_acronym_str ABC-1
network_name_str Anais da Academia Brasileira de Ciências (Online)
repository_id_str
spelling Bioactivity and action mechanism of green propolis against Pythium aphanidermatumgreen propolisArtepillin CfungicidePythium aphanidermatummodel membranesAbstract: We have established how natural compounds from green propolis collected by the species Apis mellifera act against the growth of Pythium aphanidermatum. On the basis of mass spectrometry (Q-ToF MS), we determined that Artepillin C, the major constituent of green propolis, underlies the effect and displays activity against P. aphanidermatum at a minimal inhibitory concentration of 750 µg.mL-1. Biophysical studies based on model membranes showed that this inhibitory effect may be linked with a membrane-related phenomenon: Artepillin C increases the permeability of membranes with relatively high fluidity in their lateral structure, a feature that is in line with the lipid composition reported for the cytoplasmic membrane of P. aphanidermatum. Therefore, the present study supports the use of the effective and inexpensive green propolis to control the impact of the dangerous phytopathogen P. aphanidermatum on agriculture.Academia Brasileira de Ciências2019-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000300617Anais da Academia Brasileira de Ciências v.91 n.2 2019reponame:Anais da Academia Brasileira de Ciências (Online)instname:Academia Brasileira de Ciências (ABC)instacron:ABC10.1590/0001-3765201920180598info:eu-repo/semantics/openAccessPAZIN,WALLANCE M.SANTOS,SUIKINAI N. DOSQUEIROZ,SONIA C.N.BAGATOLLI,LUIS A.SOARES,ADEMILSON E.E.MELO,ITAMAR S. DEITO,AMANDO S.eng2019-06-27T00:00:00Zoai:scielo:S0001-37652019000300617Revistahttp://www.scielo.br/aabchttps://old.scielo.br/oai/scielo-oai.php||aabc@abc.org.br1678-26900001-3765opendoar:2019-06-27T00:00Anais da Academia Brasileira de Ciências (Online) - Academia Brasileira de Ciências (ABC)false
dc.title.none.fl_str_mv Bioactivity and action mechanism of green propolis against Pythium aphanidermatum
title Bioactivity and action mechanism of green propolis against Pythium aphanidermatum
spellingShingle Bioactivity and action mechanism of green propolis against Pythium aphanidermatum
PAZIN,WALLANCE M.
green propolis
Artepillin C
fungicide
Pythium aphanidermatum
model membranes
title_short Bioactivity and action mechanism of green propolis against Pythium aphanidermatum
title_full Bioactivity and action mechanism of green propolis against Pythium aphanidermatum
title_fullStr Bioactivity and action mechanism of green propolis against Pythium aphanidermatum
title_full_unstemmed Bioactivity and action mechanism of green propolis against Pythium aphanidermatum
title_sort Bioactivity and action mechanism of green propolis against Pythium aphanidermatum
author PAZIN,WALLANCE M.
author_facet PAZIN,WALLANCE M.
SANTOS,SUIKINAI N. DOS
QUEIROZ,SONIA C.N.
BAGATOLLI,LUIS A.
SOARES,ADEMILSON E.E.
MELO,ITAMAR S. DE
ITO,AMANDO S.
author_role author
author2 SANTOS,SUIKINAI N. DOS
QUEIROZ,SONIA C.N.
BAGATOLLI,LUIS A.
SOARES,ADEMILSON E.E.
MELO,ITAMAR S. DE
ITO,AMANDO S.
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv PAZIN,WALLANCE M.
SANTOS,SUIKINAI N. DOS
QUEIROZ,SONIA C.N.
BAGATOLLI,LUIS A.
SOARES,ADEMILSON E.E.
MELO,ITAMAR S. DE
ITO,AMANDO S.
dc.subject.por.fl_str_mv green propolis
Artepillin C
fungicide
Pythium aphanidermatum
model membranes
topic green propolis
Artepillin C
fungicide
Pythium aphanidermatum
model membranes
description Abstract: We have established how natural compounds from green propolis collected by the species Apis mellifera act against the growth of Pythium aphanidermatum. On the basis of mass spectrometry (Q-ToF MS), we determined that Artepillin C, the major constituent of green propolis, underlies the effect and displays activity against P. aphanidermatum at a minimal inhibitory concentration of 750 µg.mL-1. Biophysical studies based on model membranes showed that this inhibitory effect may be linked with a membrane-related phenomenon: Artepillin C increases the permeability of membranes with relatively high fluidity in their lateral structure, a feature that is in line with the lipid composition reported for the cytoplasmic membrane of P. aphanidermatum. Therefore, the present study supports the use of the effective and inexpensive green propolis to control the impact of the dangerous phytopathogen P. aphanidermatum on agriculture.
publishDate 2019
dc.date.none.fl_str_mv 2019-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000300617
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000300617
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/0001-3765201920180598
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Academia Brasileira de Ciências
publisher.none.fl_str_mv Academia Brasileira de Ciências
dc.source.none.fl_str_mv Anais da Academia Brasileira de Ciências v.91 n.2 2019
reponame:Anais da Academia Brasileira de Ciências (Online)
instname:Academia Brasileira de Ciências (ABC)
instacron:ABC
instname_str Academia Brasileira de Ciências (ABC)
instacron_str ABC
institution ABC
reponame_str Anais da Academia Brasileira de Ciências (Online)
collection Anais da Academia Brasileira de Ciências (Online)
repository.name.fl_str_mv Anais da Academia Brasileira de Ciências (Online) - Academia Brasileira de Ciências (ABC)
repository.mail.fl_str_mv ||aabc@abc.org.br
_version_ 1754302867340001280

Registros relacionados