Cytotoxicity and bacterial membrane destabilization induced by Annona squamosa L. extracts
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Anais da Academia Brasileira de Ciências (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000502053 |
Resumo: | ABSTRACT This study aimed to further investigate the cytotoxicity against tumor cell lines and several bacterial strains of Annona squamosa and its mode of action. Methanol extracts of A. squamosa leaves (ASL) and seeds (ASS) were used. ASL showed significant antibacterial activity against S. aureus, K. pneumoniae and E. faecalis with MIC values of 78, 78 and 39 µg/mL respectively. Moreover, ASL exhibited significant biofilm disruption, rapid time dependent kinetics of bacterial killing, increased membrane permeability and significantly reduced the cell numbers and viability. Regarding the cytotoxicity against tumor cell lines, ASS was more active against Jurkat and MCF-7 cells, with CI50 1.1 and 2.1 µg/mL, respectively. ASL showed promising activity against Jurkat and HL60, with CI50 4.2 and 6.4 µg/mL, respectively. Both extracts showed lower activity against VERO cells and reduced the clonogenic survival at higher concentrations (IC90) to MCF-7 and HCT-116 lineages. The alkaloids anonaine, asimilobine, corypalmine, liriodenine nornuciferine and reticuline were identified in extracts by UPLC-ESI-MS/MS analysis. This study reinforced that A. squamosa presents a remarkable phytomedicinal potential and revealed that its antimicrobial mechanism of action is related to bacterial membrane destabilization. |
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Cytotoxicity and bacterial membrane destabilization induced by Annona squamosa L. extractsalkaloidsAnnona squamosaantibacterialcitotoxicityherbal medicineABSTRACT This study aimed to further investigate the cytotoxicity against tumor cell lines and several bacterial strains of Annona squamosa and its mode of action. Methanol extracts of A. squamosa leaves (ASL) and seeds (ASS) were used. ASL showed significant antibacterial activity against S. aureus, K. pneumoniae and E. faecalis with MIC values of 78, 78 and 39 µg/mL respectively. Moreover, ASL exhibited significant biofilm disruption, rapid time dependent kinetics of bacterial killing, increased membrane permeability and significantly reduced the cell numbers and viability. Regarding the cytotoxicity against tumor cell lines, ASS was more active against Jurkat and MCF-7 cells, with CI50 1.1 and 2.1 µg/mL, respectively. ASL showed promising activity against Jurkat and HL60, with CI50 4.2 and 6.4 µg/mL, respectively. Both extracts showed lower activity against VERO cells and reduced the clonogenic survival at higher concentrations (IC90) to MCF-7 and HCT-116 lineages. The alkaloids anonaine, asimilobine, corypalmine, liriodenine nornuciferine and reticuline were identified in extracts by UPLC-ESI-MS/MS analysis. This study reinforced that A. squamosa presents a remarkable phytomedicinal potential and revealed that its antimicrobial mechanism of action is related to bacterial membrane destabilization.Academia Brasileira de Ciências2017-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000502053Anais da Academia Brasileira de Ciências v.89 n.3 suppl.0 2017reponame:Anais da Academia Brasileira de Ciências (Online)instname:Academia Brasileira de Ciências (ABC)instacron:ABC10.1590/0001-3765201720150702info:eu-repo/semantics/openAccessPINTO,NÍCOLAS C.C.SILVA,JUCÉLIA B.MENEGATI,LAURA M.GUEDES,MARIA CLARA M.R.MARQUES,LUCAS B.SILVA,THIAGO P. DAMELO,ROSSANA C.N. DESOUZA-FAGUNDES,ELAINE M. DESALVADOR,MARCOS J.SCIO,ELITAFABRI,RODRIGO L.eng2017-11-13T00:00:00Zoai:scielo:S0001-37652017000502053Revistahttp://www.scielo.br/aabchttps://old.scielo.br/oai/scielo-oai.php||aabc@abc.org.br1678-26900001-3765opendoar:2017-11-13T00:00Anais da Academia Brasileira de Ciências (Online) - Academia Brasileira de Ciências (ABC)false |
dc.title.none.fl_str_mv |
Cytotoxicity and bacterial membrane destabilization induced by Annona squamosa L. extracts |
title |
Cytotoxicity and bacterial membrane destabilization induced by Annona squamosa L. extracts |
spellingShingle |
Cytotoxicity and bacterial membrane destabilization induced by Annona squamosa L. extracts PINTO,NÍCOLAS C.C. alkaloids Annona squamosa antibacterial citotoxicity herbal medicine |
title_short |
Cytotoxicity and bacterial membrane destabilization induced by Annona squamosa L. extracts |
title_full |
Cytotoxicity and bacterial membrane destabilization induced by Annona squamosa L. extracts |
title_fullStr |
Cytotoxicity and bacterial membrane destabilization induced by Annona squamosa L. extracts |
title_full_unstemmed |
Cytotoxicity and bacterial membrane destabilization induced by Annona squamosa L. extracts |
title_sort |
Cytotoxicity and bacterial membrane destabilization induced by Annona squamosa L. extracts |
author |
PINTO,NÍCOLAS C.C. |
author_facet |
PINTO,NÍCOLAS C.C. SILVA,JUCÉLIA B. MENEGATI,LAURA M. GUEDES,MARIA CLARA M.R. MARQUES,LUCAS B. SILVA,THIAGO P. DA MELO,ROSSANA C.N. DE SOUZA-FAGUNDES,ELAINE M. DE SALVADOR,MARCOS J. SCIO,ELITA FABRI,RODRIGO L. |
author_role |
author |
author2 |
SILVA,JUCÉLIA B. MENEGATI,LAURA M. GUEDES,MARIA CLARA M.R. MARQUES,LUCAS B. SILVA,THIAGO P. DA MELO,ROSSANA C.N. DE SOUZA-FAGUNDES,ELAINE M. DE SALVADOR,MARCOS J. SCIO,ELITA FABRI,RODRIGO L. |
author2_role |
author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
PINTO,NÍCOLAS C.C. SILVA,JUCÉLIA B. MENEGATI,LAURA M. GUEDES,MARIA CLARA M.R. MARQUES,LUCAS B. SILVA,THIAGO P. DA MELO,ROSSANA C.N. DE SOUZA-FAGUNDES,ELAINE M. DE SALVADOR,MARCOS J. SCIO,ELITA FABRI,RODRIGO L. |
dc.subject.por.fl_str_mv |
alkaloids Annona squamosa antibacterial citotoxicity herbal medicine |
topic |
alkaloids Annona squamosa antibacterial citotoxicity herbal medicine |
description |
ABSTRACT This study aimed to further investigate the cytotoxicity against tumor cell lines and several bacterial strains of Annona squamosa and its mode of action. Methanol extracts of A. squamosa leaves (ASL) and seeds (ASS) were used. ASL showed significant antibacterial activity against S. aureus, K. pneumoniae and E. faecalis with MIC values of 78, 78 and 39 µg/mL respectively. Moreover, ASL exhibited significant biofilm disruption, rapid time dependent kinetics of bacterial killing, increased membrane permeability and significantly reduced the cell numbers and viability. Regarding the cytotoxicity against tumor cell lines, ASS was more active against Jurkat and MCF-7 cells, with CI50 1.1 and 2.1 µg/mL, respectively. ASL showed promising activity against Jurkat and HL60, with CI50 4.2 and 6.4 µg/mL, respectively. Both extracts showed lower activity against VERO cells and reduced the clonogenic survival at higher concentrations (IC90) to MCF-7 and HCT-116 lineages. The alkaloids anonaine, asimilobine, corypalmine, liriodenine nornuciferine and reticuline were identified in extracts by UPLC-ESI-MS/MS analysis. This study reinforced that A. squamosa presents a remarkable phytomedicinal potential and revealed that its antimicrobial mechanism of action is related to bacterial membrane destabilization. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000502053 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000502053 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/0001-3765201720150702 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Academia Brasileira de Ciências |
publisher.none.fl_str_mv |
Academia Brasileira de Ciências |
dc.source.none.fl_str_mv |
Anais da Academia Brasileira de Ciências v.89 n.3 suppl.0 2017 reponame:Anais da Academia Brasileira de Ciências (Online) instname:Academia Brasileira de Ciências (ABC) instacron:ABC |
instname_str |
Academia Brasileira de Ciências (ABC) |
instacron_str |
ABC |
institution |
ABC |
reponame_str |
Anais da Academia Brasileira de Ciências (Online) |
collection |
Anais da Academia Brasileira de Ciências (Online) |
repository.name.fl_str_mv |
Anais da Academia Brasileira de Ciências (Online) - Academia Brasileira de Ciências (ABC) |
repository.mail.fl_str_mv |
||aabc@abc.org.br |
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1754302864807690240 |