DNA damage in children and adolescents with cardiovascular disease risk factors

Detalhes bibliográficos
Autor(a) principal: Kliemann,Mariele
Data de Publicação: 2012
Outros Autores: Prá,Daniel, Müller,Luiza L., Hermes,Liziane, Horta,Jorge A., Reckziegel,Miriam B., Burgos,Miria S., Maluf,Sharbel W., Franke,Silvia I.R., Silva,Juliana da
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Anais da Academia Brasileira de Ciências (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652012000300025
Resumo: The risk of developing cardiovascular disease (CVD) is related to lifestyle (e.g. diet, physical activity and smoking) as well as to genetic factors. This study aimed at evaluating the association between CVD risk factors and DNA damage levels in children and adolescents. Anthropometry, diet and serum CVD risk factors were evaluated by standard procedures. DNA damage levels were accessed by the comet assay (Single cell gel electrophoresis; SCGE) and cytokinesis-blocked micronucleus (CBMN) assays in leukocytes. A total of 34 children and adolescents selected from a population sample were divided into three groups according to their level of CVD risk. Moderate and high CVD risk subjects showed significantly higher body fat and serum CVD risk markers than low risk subjects (P<0.05). High risk subjects also showed a significant increase in DNA damage, which was higher than that provided by low and moderate risk subjects according to SCGE, but not according to the CBMN assay. Vitamin C intake was inversely correlated with DNA damage by SCGE, and micronucleus (MN) was inversely correlated with folate intake. The present results indicate an increase in DNA damage that may be a consequence of oxidative stress in young individuals with risk factors for CVD, indicating that the DNA damage level can aid in evaluating the risk of CVD.
id ABC-1_d87bfc9a2017b685046d12f2f679fc69
oai_identifier_str oai:scielo:S0001-37652012000300025
network_acronym_str ABC-1
network_name_str Anais da Academia Brasileira de Ciências (Online)
repository_id_str
spelling DNA damage in children and adolescents with cardiovascular disease risk factorscardiovascular diseasesMicronucleus testComet assaydietanthropometrychildadolescentThe risk of developing cardiovascular disease (CVD) is related to lifestyle (e.g. diet, physical activity and smoking) as well as to genetic factors. This study aimed at evaluating the association between CVD risk factors and DNA damage levels in children and adolescents. Anthropometry, diet and serum CVD risk factors were evaluated by standard procedures. DNA damage levels were accessed by the comet assay (Single cell gel electrophoresis; SCGE) and cytokinesis-blocked micronucleus (CBMN) assays in leukocytes. A total of 34 children and adolescents selected from a population sample were divided into three groups according to their level of CVD risk. Moderate and high CVD risk subjects showed significantly higher body fat and serum CVD risk markers than low risk subjects (P<0.05). High risk subjects also showed a significant increase in DNA damage, which was higher than that provided by low and moderate risk subjects according to SCGE, but not according to the CBMN assay. Vitamin C intake was inversely correlated with DNA damage by SCGE, and micronucleus (MN) was inversely correlated with folate intake. The present results indicate an increase in DNA damage that may be a consequence of oxidative stress in young individuals with risk factors for CVD, indicating that the DNA damage level can aid in evaluating the risk of CVD.Academia Brasileira de Ciências2012-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652012000300025Anais da Academia Brasileira de Ciências v.84 n.3 2012reponame:Anais da Academia Brasileira de Ciências (Online)instname:Academia Brasileira de Ciências (ABC)instacron:ABC10.1590/S0001-37652012005000039info:eu-repo/semantics/openAccessKliemann,MarielePrá,DanielMüller,Luiza L.Hermes,LizianeHorta,Jorge A.Reckziegel,Miriam B.Burgos,Miria S.Maluf,Sharbel W.Franke,Silvia I.R.Silva,Juliana daeng2012-08-06T00:00:00Zoai:scielo:S0001-37652012000300025Revistahttp://www.scielo.br/aabchttps://old.scielo.br/oai/scielo-oai.php||aabc@abc.org.br1678-26900001-3765opendoar:2012-08-06T00:00Anais da Academia Brasileira de Ciências (Online) - Academia Brasileira de Ciências (ABC)false
dc.title.none.fl_str_mv DNA damage in children and adolescents with cardiovascular disease risk factors
title DNA damage in children and adolescents with cardiovascular disease risk factors
spellingShingle DNA damage in children and adolescents with cardiovascular disease risk factors
Kliemann,Mariele
cardiovascular diseases
Micronucleus test
Comet assay
diet
anthropometry
child
adolescent
title_short DNA damage in children and adolescents with cardiovascular disease risk factors
title_full DNA damage in children and adolescents with cardiovascular disease risk factors
title_fullStr DNA damage in children and adolescents with cardiovascular disease risk factors
title_full_unstemmed DNA damage in children and adolescents with cardiovascular disease risk factors
title_sort DNA damage in children and adolescents with cardiovascular disease risk factors
author Kliemann,Mariele
author_facet Kliemann,Mariele
Prá,Daniel
Müller,Luiza L.
Hermes,Liziane
Horta,Jorge A.
Reckziegel,Miriam B.
Burgos,Miria S.
Maluf,Sharbel W.
Franke,Silvia I.R.
Silva,Juliana da
author_role author
author2 Prá,Daniel
Müller,Luiza L.
Hermes,Liziane
Horta,Jorge A.
Reckziegel,Miriam B.
Burgos,Miria S.
Maluf,Sharbel W.
Franke,Silvia I.R.
Silva,Juliana da
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Kliemann,Mariele
Prá,Daniel
Müller,Luiza L.
Hermes,Liziane
Horta,Jorge A.
Reckziegel,Miriam B.
Burgos,Miria S.
Maluf,Sharbel W.
Franke,Silvia I.R.
Silva,Juliana da
dc.subject.por.fl_str_mv cardiovascular diseases
Micronucleus test
Comet assay
diet
anthropometry
child
adolescent
topic cardiovascular diseases
Micronucleus test
Comet assay
diet
anthropometry
child
adolescent
description The risk of developing cardiovascular disease (CVD) is related to lifestyle (e.g. diet, physical activity and smoking) as well as to genetic factors. This study aimed at evaluating the association between CVD risk factors and DNA damage levels in children and adolescents. Anthropometry, diet and serum CVD risk factors were evaluated by standard procedures. DNA damage levels were accessed by the comet assay (Single cell gel electrophoresis; SCGE) and cytokinesis-blocked micronucleus (CBMN) assays in leukocytes. A total of 34 children and adolescents selected from a population sample were divided into three groups according to their level of CVD risk. Moderate and high CVD risk subjects showed significantly higher body fat and serum CVD risk markers than low risk subjects (P<0.05). High risk subjects also showed a significant increase in DNA damage, which was higher than that provided by low and moderate risk subjects according to SCGE, but not according to the CBMN assay. Vitamin C intake was inversely correlated with DNA damage by SCGE, and micronucleus (MN) was inversely correlated with folate intake. The present results indicate an increase in DNA damage that may be a consequence of oxidative stress in young individuals with risk factors for CVD, indicating that the DNA damage level can aid in evaluating the risk of CVD.
publishDate 2012
dc.date.none.fl_str_mv 2012-09-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652012000300025
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652012000300025
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0001-37652012005000039
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Academia Brasileira de Ciências
publisher.none.fl_str_mv Academia Brasileira de Ciências
dc.source.none.fl_str_mv Anais da Academia Brasileira de Ciências v.84 n.3 2012
reponame:Anais da Academia Brasileira de Ciências (Online)
instname:Academia Brasileira de Ciências (ABC)
instacron:ABC
instname_str Academia Brasileira de Ciências (ABC)
instacron_str ABC
institution ABC
reponame_str Anais da Academia Brasileira de Ciências (Online)
collection Anais da Academia Brasileira de Ciências (Online)
repository.name.fl_str_mv Anais da Academia Brasileira de Ciências (Online) - Academia Brasileira de Ciências (ABC)
repository.mail.fl_str_mv ||aabc@abc.org.br
_version_ 1754302858853875712