DNA damage in children and adolescents with cardiovascular disease risk factors
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Anais da Academia Brasileira de Ciências (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652012000300025 |
Resumo: | The risk of developing cardiovascular disease (CVD) is related to lifestyle (e.g. diet, physical activity and smoking) as well as to genetic factors. This study aimed at evaluating the association between CVD risk factors and DNA damage levels in children and adolescents. Anthropometry, diet and serum CVD risk factors were evaluated by standard procedures. DNA damage levels were accessed by the comet assay (Single cell gel electrophoresis; SCGE) and cytokinesis-blocked micronucleus (CBMN) assays in leukocytes. A total of 34 children and adolescents selected from a population sample were divided into three groups according to their level of CVD risk. Moderate and high CVD risk subjects showed significantly higher body fat and serum CVD risk markers than low risk subjects (P<0.05). High risk subjects also showed a significant increase in DNA damage, which was higher than that provided by low and moderate risk subjects according to SCGE, but not according to the CBMN assay. Vitamin C intake was inversely correlated with DNA damage by SCGE, and micronucleus (MN) was inversely correlated with folate intake. The present results indicate an increase in DNA damage that may be a consequence of oxidative stress in young individuals with risk factors for CVD, indicating that the DNA damage level can aid in evaluating the risk of CVD. |
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DNA damage in children and adolescents with cardiovascular disease risk factorscardiovascular diseasesMicronucleus testComet assaydietanthropometrychildadolescentThe risk of developing cardiovascular disease (CVD) is related to lifestyle (e.g. diet, physical activity and smoking) as well as to genetic factors. This study aimed at evaluating the association between CVD risk factors and DNA damage levels in children and adolescents. Anthropometry, diet and serum CVD risk factors were evaluated by standard procedures. DNA damage levels were accessed by the comet assay (Single cell gel electrophoresis; SCGE) and cytokinesis-blocked micronucleus (CBMN) assays in leukocytes. A total of 34 children and adolescents selected from a population sample were divided into three groups according to their level of CVD risk. Moderate and high CVD risk subjects showed significantly higher body fat and serum CVD risk markers than low risk subjects (P<0.05). High risk subjects also showed a significant increase in DNA damage, which was higher than that provided by low and moderate risk subjects according to SCGE, but not according to the CBMN assay. Vitamin C intake was inversely correlated with DNA damage by SCGE, and micronucleus (MN) was inversely correlated with folate intake. The present results indicate an increase in DNA damage that may be a consequence of oxidative stress in young individuals with risk factors for CVD, indicating that the DNA damage level can aid in evaluating the risk of CVD.Academia Brasileira de Ciências2012-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652012000300025Anais da Academia Brasileira de Ciências v.84 n.3 2012reponame:Anais da Academia Brasileira de Ciências (Online)instname:Academia Brasileira de Ciências (ABC)instacron:ABC10.1590/S0001-37652012005000039info:eu-repo/semantics/openAccessKliemann,MarielePrá,DanielMüller,Luiza L.Hermes,LizianeHorta,Jorge A.Reckziegel,Miriam B.Burgos,Miria S.Maluf,Sharbel W.Franke,Silvia I.R.Silva,Juliana daeng2012-08-06T00:00:00Zoai:scielo:S0001-37652012000300025Revistahttp://www.scielo.br/aabchttps://old.scielo.br/oai/scielo-oai.php||aabc@abc.org.br1678-26900001-3765opendoar:2012-08-06T00:00Anais da Academia Brasileira de Ciências (Online) - Academia Brasileira de Ciências (ABC)false |
dc.title.none.fl_str_mv |
DNA damage in children and adolescents with cardiovascular disease risk factors |
title |
DNA damage in children and adolescents with cardiovascular disease risk factors |
spellingShingle |
DNA damage in children and adolescents with cardiovascular disease risk factors Kliemann,Mariele cardiovascular diseases Micronucleus test Comet assay diet anthropometry child adolescent |
title_short |
DNA damage in children and adolescents with cardiovascular disease risk factors |
title_full |
DNA damage in children and adolescents with cardiovascular disease risk factors |
title_fullStr |
DNA damage in children and adolescents with cardiovascular disease risk factors |
title_full_unstemmed |
DNA damage in children and adolescents with cardiovascular disease risk factors |
title_sort |
DNA damage in children and adolescents with cardiovascular disease risk factors |
author |
Kliemann,Mariele |
author_facet |
Kliemann,Mariele Prá,Daniel Müller,Luiza L. Hermes,Liziane Horta,Jorge A. Reckziegel,Miriam B. Burgos,Miria S. Maluf,Sharbel W. Franke,Silvia I.R. Silva,Juliana da |
author_role |
author |
author2 |
Prá,Daniel Müller,Luiza L. Hermes,Liziane Horta,Jorge A. Reckziegel,Miriam B. Burgos,Miria S. Maluf,Sharbel W. Franke,Silvia I.R. Silva,Juliana da |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Kliemann,Mariele Prá,Daniel Müller,Luiza L. Hermes,Liziane Horta,Jorge A. Reckziegel,Miriam B. Burgos,Miria S. Maluf,Sharbel W. Franke,Silvia I.R. Silva,Juliana da |
dc.subject.por.fl_str_mv |
cardiovascular diseases Micronucleus test Comet assay diet anthropometry child adolescent |
topic |
cardiovascular diseases Micronucleus test Comet assay diet anthropometry child adolescent |
description |
The risk of developing cardiovascular disease (CVD) is related to lifestyle (e.g. diet, physical activity and smoking) as well as to genetic factors. This study aimed at evaluating the association between CVD risk factors and DNA damage levels in children and adolescents. Anthropometry, diet and serum CVD risk factors were evaluated by standard procedures. DNA damage levels were accessed by the comet assay (Single cell gel electrophoresis; SCGE) and cytokinesis-blocked micronucleus (CBMN) assays in leukocytes. A total of 34 children and adolescents selected from a population sample were divided into three groups according to their level of CVD risk. Moderate and high CVD risk subjects showed significantly higher body fat and serum CVD risk markers than low risk subjects (P<0.05). High risk subjects also showed a significant increase in DNA damage, which was higher than that provided by low and moderate risk subjects according to SCGE, but not according to the CBMN assay. Vitamin C intake was inversely correlated with DNA damage by SCGE, and micronucleus (MN) was inversely correlated with folate intake. The present results indicate an increase in DNA damage that may be a consequence of oxidative stress in young individuals with risk factors for CVD, indicating that the DNA damage level can aid in evaluating the risk of CVD. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-09-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652012000300025 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652012000300025 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0001-37652012005000039 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Academia Brasileira de Ciências |
publisher.none.fl_str_mv |
Academia Brasileira de Ciências |
dc.source.none.fl_str_mv |
Anais da Academia Brasileira de Ciências v.84 n.3 2012 reponame:Anais da Academia Brasileira de Ciências (Online) instname:Academia Brasileira de Ciências (ABC) instacron:ABC |
instname_str |
Academia Brasileira de Ciências (ABC) |
instacron_str |
ABC |
institution |
ABC |
reponame_str |
Anais da Academia Brasileira de Ciências (Online) |
collection |
Anais da Academia Brasileira de Ciências (Online) |
repository.name.fl_str_mv |
Anais da Academia Brasileira de Ciências (Online) - Academia Brasileira de Ciências (ABC) |
repository.mail.fl_str_mv |
||aabc@abc.org.br |
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1754302858853875712 |