Validation of a liquid chromatography ultraviolet method for determination of herbicide diuron and its metabolites in soil samples
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Anais da Academia Brasileira de Ciências (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000401235 |
Resumo: | ABSTRACT Diuron is one of the most widely herbicide used worldwide, which can undergo degradation producing three primary metabolites: 3,4-dichlorophenylurea, 3-(3,4-dichlorophenyl)-1-methylurea, and 3,4-dichloroaniline. Since the persistence of diuron and its by-products in ecosystems involves risk of toxicity to environment and human health, a reliable quantitative method for simultaneous monitoring of these compounds is required. Hence, a simple method without preconcentration step was validated for quantitation of diuron and its main metabolites by high performance liquid chromatography with ultraviolet detection. Separation was achieved in less than 11 minutes using a C18 column, mobile phase composed of acetonitrile and water (45:55 v/v) at 0.86 mL min-1 and detection at 254 nm. The validated method using solid-liquid extraction followed by an isocratic chromatographic elution proved to be specific, precise and linear (R2 ˃ 0.99), presenting more than 90% of recovery. The method was successfully applied to quantify diuron and their by-products in soil samples collected in a sugarcane cultivation area, focusing on the environmental control. |
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Anais da Academia Brasileira de Ciências (Online) |
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Validation of a liquid chromatography ultraviolet method for determination of herbicide diuron and its metabolites in soil samplespesticidesdegradation productsHPLC-UVsoilenvironmental controlABSTRACT Diuron is one of the most widely herbicide used worldwide, which can undergo degradation producing three primary metabolites: 3,4-dichlorophenylurea, 3-(3,4-dichlorophenyl)-1-methylurea, and 3,4-dichloroaniline. Since the persistence of diuron and its by-products in ecosystems involves risk of toxicity to environment and human health, a reliable quantitative method for simultaneous monitoring of these compounds is required. Hence, a simple method without preconcentration step was validated for quantitation of diuron and its main metabolites by high performance liquid chromatography with ultraviolet detection. Separation was achieved in less than 11 minutes using a C18 column, mobile phase composed of acetonitrile and water (45:55 v/v) at 0.86 mL min-1 and detection at 254 nm. The validated method using solid-liquid extraction followed by an isocratic chromatographic elution proved to be specific, precise and linear (R2 ˃ 0.99), presenting more than 90% of recovery. The method was successfully applied to quantify diuron and their by-products in soil samples collected in a sugarcane cultivation area, focusing on the environmental control.Academia Brasileira de Ciências2016-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000401235Anais da Academia Brasileira de Ciências v.88 n.3 2016reponame:Anais da Academia Brasileira de Ciências (Online)instname:Academia Brasileira de Ciências (ABC)instacron:ABC10.1590/0001-3765201620150234info:eu-repo/semantics/openAccessFELICIO,ANA LUCIA S.M.MONTEIRO,ALESSANDRA M.ALMEIDA,MARIANA B.MADEIRA,TIAGO B.NIXDORF,SUZANA L.YABE,MARIA JOSEFA S.eng2016-09-12T00:00:00Zoai:scielo:S0001-37652016000401235Revistahttp://www.scielo.br/aabchttps://old.scielo.br/oai/scielo-oai.php||aabc@abc.org.br1678-26900001-3765opendoar:2016-09-12T00:00Anais da Academia Brasileira de Ciências (Online) - Academia Brasileira de Ciências (ABC)false |
dc.title.none.fl_str_mv |
Validation of a liquid chromatography ultraviolet method for determination of herbicide diuron and its metabolites in soil samples |
title |
Validation of a liquid chromatography ultraviolet method for determination of herbicide diuron and its metabolites in soil samples |
spellingShingle |
Validation of a liquid chromatography ultraviolet method for determination of herbicide diuron and its metabolites in soil samples FELICIO,ANA LUCIA S.M. pesticides degradation products HPLC-UV soil environmental control |
title_short |
Validation of a liquid chromatography ultraviolet method for determination of herbicide diuron and its metabolites in soil samples |
title_full |
Validation of a liquid chromatography ultraviolet method for determination of herbicide diuron and its metabolites in soil samples |
title_fullStr |
Validation of a liquid chromatography ultraviolet method for determination of herbicide diuron and its metabolites in soil samples |
title_full_unstemmed |
Validation of a liquid chromatography ultraviolet method for determination of herbicide diuron and its metabolites in soil samples |
title_sort |
Validation of a liquid chromatography ultraviolet method for determination of herbicide diuron and its metabolites in soil samples |
author |
FELICIO,ANA LUCIA S.M. |
author_facet |
FELICIO,ANA LUCIA S.M. MONTEIRO,ALESSANDRA M. ALMEIDA,MARIANA B. MADEIRA,TIAGO B. NIXDORF,SUZANA L. YABE,MARIA JOSEFA S. |
author_role |
author |
author2 |
MONTEIRO,ALESSANDRA M. ALMEIDA,MARIANA B. MADEIRA,TIAGO B. NIXDORF,SUZANA L. YABE,MARIA JOSEFA S. |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
FELICIO,ANA LUCIA S.M. MONTEIRO,ALESSANDRA M. ALMEIDA,MARIANA B. MADEIRA,TIAGO B. NIXDORF,SUZANA L. YABE,MARIA JOSEFA S. |
dc.subject.por.fl_str_mv |
pesticides degradation products HPLC-UV soil environmental control |
topic |
pesticides degradation products HPLC-UV soil environmental control |
description |
ABSTRACT Diuron is one of the most widely herbicide used worldwide, which can undergo degradation producing three primary metabolites: 3,4-dichlorophenylurea, 3-(3,4-dichlorophenyl)-1-methylurea, and 3,4-dichloroaniline. Since the persistence of diuron and its by-products in ecosystems involves risk of toxicity to environment and human health, a reliable quantitative method for simultaneous monitoring of these compounds is required. Hence, a simple method without preconcentration step was validated for quantitation of diuron and its main metabolites by high performance liquid chromatography with ultraviolet detection. Separation was achieved in less than 11 minutes using a C18 column, mobile phase composed of acetonitrile and water (45:55 v/v) at 0.86 mL min-1 and detection at 254 nm. The validated method using solid-liquid extraction followed by an isocratic chromatographic elution proved to be specific, precise and linear (R2 ˃ 0.99), presenting more than 90% of recovery. The method was successfully applied to quantify diuron and their by-products in soil samples collected in a sugarcane cultivation area, focusing on the environmental control. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-09-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000401235 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000401235 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/0001-3765201620150234 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Academia Brasileira de Ciências |
publisher.none.fl_str_mv |
Academia Brasileira de Ciências |
dc.source.none.fl_str_mv |
Anais da Academia Brasileira de Ciências v.88 n.3 2016 reponame:Anais da Academia Brasileira de Ciências (Online) instname:Academia Brasileira de Ciências (ABC) instacron:ABC |
instname_str |
Academia Brasileira de Ciências (ABC) |
instacron_str |
ABC |
institution |
ABC |
reponame_str |
Anais da Academia Brasileira de Ciências (Online) |
collection |
Anais da Academia Brasileira de Ciências (Online) |
repository.name.fl_str_mv |
Anais da Academia Brasileira de Ciências (Online) - Academia Brasileira de Ciências (ABC) |
repository.mail.fl_str_mv |
||aabc@abc.org.br |
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1754302863150940160 |