Renoprotective effect of N-acetylcysteine depends upon the severity of the ischemia reperfusion injury
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Medical and Biological Research |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2021001100608 |
Resumo: | Acute kidney injury (AKI) is a common complication in seriously ill patients, while renal ischemia-reperfusion (I/R) injury is the most frequent event in this oxidative renal injury. N-acetylcysteine (NAC) is a small molecule containing a thiol group that has antioxidant properties, promoting detoxification and acting directly as a free radical scavenger. In this study, the protective effect of NAC was investigated in short-term (30 min) and long-term (45 min) ischemic AKI. This was achieved via clamping of the renal artery for 30 or 45 min in Wistar rats to induce I/R injury. AKI worsened with a longer period of ischemia (45 compared to 30 min) due to probable irreversible damage. Preconditioning with NAC in short-term ischemia improved renal blood flow and increased creatinine clearance by reducing oxidative metabolites and increasing antioxidant capacity. Otherwise, NAC did not change these parameters in the long-term ischemia. Therefore, this study demonstrated that the period of ischemia determines the severity of the AKI, and NAC presented antioxidant effects in short-term ischemia but not in long-term ischemia, confirming that there is a possible therapeutic window for its renoprotective effect. |
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Renoprotective effect of N-acetylcysteine depends upon the severity of the ischemia reperfusion injuryAcute kidney injuryOxidative stressIschemia-reperfusionAcute kidney injury (AKI) is a common complication in seriously ill patients, while renal ischemia-reperfusion (I/R) injury is the most frequent event in this oxidative renal injury. N-acetylcysteine (NAC) is a small molecule containing a thiol group that has antioxidant properties, promoting detoxification and acting directly as a free radical scavenger. In this study, the protective effect of NAC was investigated in short-term (30 min) and long-term (45 min) ischemic AKI. This was achieved via clamping of the renal artery for 30 or 45 min in Wistar rats to induce I/R injury. AKI worsened with a longer period of ischemia (45 compared to 30 min) due to probable irreversible damage. Preconditioning with NAC in short-term ischemia improved renal blood flow and increased creatinine clearance by reducing oxidative metabolites and increasing antioxidant capacity. Otherwise, NAC did not change these parameters in the long-term ischemia. Therefore, this study demonstrated that the period of ischemia determines the severity of the AKI, and NAC presented antioxidant effects in short-term ischemia but not in long-term ischemia, confirming that there is a possible therapeutic window for its renoprotective effect.Associação Brasileira de Divulgação Científica2021-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2021001100608Brazilian Journal of Medical and Biological Research v.54 n.11 2021reponame:Brazilian Journal of Medical and Biological Researchinstname:Associação Brasileira de Divulgação Científica (ABDC)instacron:ABDC10.1590/1414-431x2021e9941info:eu-repo/semantics/openAccessWatanabe,M.Borges,F.T.Pessoa,E.A.Fonseca,C.D.Fernandes,S.M.Drew,R.C.Volpini,R.A.Vattimo,M.F.F.eng2021-09-01T00:00:00Zoai:scielo:S0100-879X2021001100608Revistahttps://www.bjournal.org/https://old.scielo.br/oai/scielo-oai.phpbjournal@terra.com.br||bjournal@terra.com.br1414-431X0100-879Xopendoar:2021-09-01T00:00Brazilian Journal of Medical and Biological Research - Associação Brasileira de Divulgação Científica (ABDC)false |
dc.title.none.fl_str_mv |
Renoprotective effect of N-acetylcysteine depends upon the severity of the ischemia reperfusion injury |
title |
Renoprotective effect of N-acetylcysteine depends upon the severity of the ischemia reperfusion injury |
spellingShingle |
Renoprotective effect of N-acetylcysteine depends upon the severity of the ischemia reperfusion injury Watanabe,M. Acute kidney injury Oxidative stress Ischemia-reperfusion |
title_short |
Renoprotective effect of N-acetylcysteine depends upon the severity of the ischemia reperfusion injury |
title_full |
Renoprotective effect of N-acetylcysteine depends upon the severity of the ischemia reperfusion injury |
title_fullStr |
Renoprotective effect of N-acetylcysteine depends upon the severity of the ischemia reperfusion injury |
title_full_unstemmed |
Renoprotective effect of N-acetylcysteine depends upon the severity of the ischemia reperfusion injury |
title_sort |
Renoprotective effect of N-acetylcysteine depends upon the severity of the ischemia reperfusion injury |
author |
Watanabe,M. |
author_facet |
Watanabe,M. Borges,F.T. Pessoa,E.A. Fonseca,C.D. Fernandes,S.M. Drew,R.C. Volpini,R.A. Vattimo,M.F.F. |
author_role |
author |
author2 |
Borges,F.T. Pessoa,E.A. Fonseca,C.D. Fernandes,S.M. Drew,R.C. Volpini,R.A. Vattimo,M.F.F. |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Watanabe,M. Borges,F.T. Pessoa,E.A. Fonseca,C.D. Fernandes,S.M. Drew,R.C. Volpini,R.A. Vattimo,M.F.F. |
dc.subject.por.fl_str_mv |
Acute kidney injury Oxidative stress Ischemia-reperfusion |
topic |
Acute kidney injury Oxidative stress Ischemia-reperfusion |
description |
Acute kidney injury (AKI) is a common complication in seriously ill patients, while renal ischemia-reperfusion (I/R) injury is the most frequent event in this oxidative renal injury. N-acetylcysteine (NAC) is a small molecule containing a thiol group that has antioxidant properties, promoting detoxification and acting directly as a free radical scavenger. In this study, the protective effect of NAC was investigated in short-term (30 min) and long-term (45 min) ischemic AKI. This was achieved via clamping of the renal artery for 30 or 45 min in Wistar rats to induce I/R injury. AKI worsened with a longer period of ischemia (45 compared to 30 min) due to probable irreversible damage. Preconditioning with NAC in short-term ischemia improved renal blood flow and increased creatinine clearance by reducing oxidative metabolites and increasing antioxidant capacity. Otherwise, NAC did not change these parameters in the long-term ischemia. Therefore, this study demonstrated that the period of ischemia determines the severity of the AKI, and NAC presented antioxidant effects in short-term ischemia but not in long-term ischemia, confirming that there is a possible therapeutic window for its renoprotective effect. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2021001100608 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2021001100608 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1414-431x2021e9941 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Associação Brasileira de Divulgação Científica |
publisher.none.fl_str_mv |
Associação Brasileira de Divulgação Científica |
dc.source.none.fl_str_mv |
Brazilian Journal of Medical and Biological Research v.54 n.11 2021 reponame:Brazilian Journal of Medical and Biological Research instname:Associação Brasileira de Divulgação Científica (ABDC) instacron:ABDC |
instname_str |
Associação Brasileira de Divulgação Científica (ABDC) |
instacron_str |
ABDC |
institution |
ABDC |
reponame_str |
Brazilian Journal of Medical and Biological Research |
collection |
Brazilian Journal of Medical and Biological Research |
repository.name.fl_str_mv |
Brazilian Journal of Medical and Biological Research - Associação Brasileira de Divulgação Científica (ABDC) |
repository.mail.fl_str_mv |
bjournal@terra.com.br||bjournal@terra.com.br |
_version_ |
1754302948575281152 |